Organs-on-chips: into the next decade

Recent advances in genome editing of stem cells for drug discovery and therapeutic application

(2020) Jaesuk Lee et al.   PHARMACOLOGY & THERAPEUTICS

Robotic fluidic coupling and interrogation of multiple vascularized organ chips

(2020) Richard Novak et al.   Nature Biomedical Engineering

Human-iPSC-Derived Cardiac Stromal Cells Enhance Maturation in 3D Cardiac Microtissues and Reveal Non-cardiomyocyte Contributions to Heart Disease

(2020) Elisa Giacomelli et al.   Cell Stem Cell

Species-specific enhancement of enterohemorrhagic E. coli pathogenesis mediated by microbiome metabolites

(2019) Alessio Tovaglieri et al.   Microbiome

Induction of Mesoderm and Neural Crest-Derived Pericytes from Human Pluripotent Stem Cells to Study Blood-Brain Barrier Interactions

(2019) Tannaz Faal et al.   Stem Cell Reports

Tubuloids derived from human adult kidney and urine for personalized disease modeling

(2019) Frans Schutgens et al.   NATURE BIOTECHNOLOGY

A Platform for Generation of Chamber-Specific Cardiac Tissues and Disease Modeling

(2019) Yimu Zhao et al.   CELL

Human-on-a-Chip Systems: Long-Term Electrical and Mechanical Function Monitoring of a Human-on-a-Chip System (Adv. Funct. Mater. 8/2019)

(2019) Carlota Oleaga et al.   ADVANCED FUNCTIONAL MATERIALS

Microfluidic Multitissue Platform for Advanced Embryotoxicity Testing In Vitro

(2019) Julia Alicia Boos et al.   Advanced Science

A complex human gut microbiome cultured in an anaerobic intestine-on-a-chip

(2019) Sasan Jalili-Firoozinezhad et al.   Nature Biomedical Engineering

Human iPSC-Derived Blood-Brain Barrier Chips Enable Disease Modeling and Personalized Medicine Applications

(2019) Gad D. Vatine et al.   Cell Stem Cell

Impact of organ-on-a-chip technology on pharmaceutical R&D costs

(2019) Nora Franzen et al.   DRUG DISCOVERY TODAY

Organoids-on-a-chip

(2019) Sunghee Estelle Park et al.   SCIENCE

Multi-organ system for the evaluation of efficacy and off-target toxicity of anticancer therapeutics

(2019) Christopher W. McAleer et al.   Science Translational Medicine

Integrating Organs-on-Chips: Multiplexing, Scaling, Vascularization, and Innervation

(2019) DoYeun Park et al.   TRENDS IN BIOTECHNOLOGY

Patient-derived pancreas-on-a-chip to model cystic fibrosis-related disorders

(2019) Kyu Shik Mun et al.   Nature Communications

Tissue Chips in Space—Challenges and Opportunities

(2019) Catherine K. Yeung et al.   CTS-Clinical and Translational Science

Methods of Delivering Mechanical Stimuli to Organ-on-a-Chip

(2019) Kaarj et al.   Micromachines

Mussel-inspired 3D fiber scaffolds for heart-on-a-chip toxicity studies of engineered nanomaterials

(2018) Seungkuk Ahn et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Organs-on-a-Chip: A Fast Track for Engineered Human Tissues in Drug Development

(2018) Kacey Ronaldson-Bouchard et al.   Cell Stem Cell

Organ-on-Chip Recapitulates Thrombosis Induced by an anti-CD154 Monoclonal Antibody: Translational Potential of Advanced Microengineered Systems

(2018) Riccardo Barrile et al.   CLINICAL PHARMACOLOGY & THERAPEUTICS

Microfluidic co-culture of pancreatic tumor spheroids with stellate cells as a novel 3D model for investigation of stroma-mediated cell motility and drug resistance

(2018) Ji-Hyun Lee et al.   JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH

A multi-throughput multi-organ-on-a-chip system on a plate formatted pneumatic pressure-driven medium circulation platform

(2018) T. Satoh et al.   LAB ON A CHIP

A Model of Dormant-Emergent Metastatic Breast Cancer Progression Enabling Exploration of Biomarker Signatures

(2018) Amanda M. Clark et al.   MOLECULAR & CELLULAR PROTEOMICS

Advanced maturation of human cardiac tissue grown from pluripotent stem cells

(2018) Kacey Ronaldson-Bouchard et al.   NATURE

The immature electrophysiological phenotype of iPSC-CMs still hampers in vitro drug screening: Special focus on I K1

(2018) Birgit Goversen et al.   PHARMACOLOGY & THERAPEUTICS

Human bone perivascular niche-on-a-chip for studying metastatic colonization

(2018) Alessandro Marturano-Kruik et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Genome Editing in Induced Pluripotent Stem Cells using CRISPR/Cas9

(2018) Ronen Ben Jehuda et al.   Stem Cell Reviews and Reports

Genome Editing in Induced Pluripotent Stem Cells using CRISPR/Cas9

(2018) Ronen Ben Jehuda et al.   Stem Cell Reviews and Reports

Human iPSC-Derived Endothelial Cells and Microengineered Organ-Chip Enhance Neuronal Development

(2018) Samuel Sances et al.   Stem Cell Reports

A 3D Human Renal Cell Carcinoma-on-a-Chip for the Study of Tumor Angiogenesis

(2018) Chris P. Miller et al.   NEOPLASIA

Investigation of the effect of hepatic metabolism on off-target cardiotoxicity in a multi-organ human-on-a-chip system

(2018) Carlota Oleaga et al.   BIOMATERIALS

A glass-based, continuously zonated and vascularized human liver acinus microphysiological system (vLAMPS) designed for experimental modeling of diseases and ADME/TOX

(2018) Xiang Li et al.   LAB ON A CHIP

A linked organ-on-chip model of the human neurovascular unit reveals the metabolic coupling of endothelial and neuronal cells

(2018) Ben M Maoz et al.   NATURE BIOTECHNOLOGY

Progress and potential in organoid research

(2018) Giuliana Rossi et al.   NATURE REVIEWS GENETICS

Interconnected Microphysiological Systems for Quantitative Biology and Pharmacology Studies

(2018) Collin D. Edington et al.   Scientific Reports

Development of a primary human Small Intestine-on-a-Chip using biopsy-derived organoids

(2018) Magdalena Kasendra et al.   Scientific Reports

Drug screening of biopsy-derived spheroids using a self-generated microfluidic concentration gradient

(2018) Theresa Mulholland et al.   Scientific Reports

Technology Transfer of the Microphysiological Systems: A Case Study of the Human Proximal Tubule Tissue Chip

(2018) Courtney Sakolish et al.   Scientific Reports

Carrier-free nanoparticles of cathepsin B-cleavable peptide-conjugated doxorubicin prodrug for cancer targeting therapy

(2018) Man Kyu Shim et al.   JOURNAL OF CONTROLLED RELEASE

The Emergence of Microphysiological Systems (Organs-on-chips) as Paradigm-changing Tools for Toxicologic Pathology

(2018) Daniel G. Rudmann   TOXICOLOGIC PATHOLOGY

Generation of pancreatic β cells for treatment of diabetes: advances and challenges

(2018) Hussain Md. Shahjalal et al.   Stem Cell Research & Therapy

A 3D human placenta-on-a-chip model to probe nanoparticle exposure at the placental barrier

(2018) Fangchao Yin et al.   TOXICOLOGY IN VITRO

Tomorrow today: organ-on-a-chip advances towards clinically relevant pharmaceutical and medical in vitro models

(2018) Mario Rothbauer et al.   CURRENT OPINION IN BIOTECHNOLOGY

Circadian hormone control in a human-on-a-chip: In vitro biology’s ignored component?

(2017) Kevin J. Cyr et al.   EXPERIMENTAL BIOLOGY AND MEDICINE

Toward improved myocardial maturity in an organ-on-chip platform with immature cardiac myocytes

(2017) Sean P Sheehy et al.   EXPERIMENTAL BIOLOGY AND MEDICINE

Control of oxygen tension recapitulates zone-specific functions in human liver microphysiology systems

(2017) Felipe T Lee-Montiel et al.   EXPERIMENTAL BIOLOGY AND MEDICINE

A vascularized and perfused organ-on-a-chip platform for large-scale drug screening applications

(2017) Duc T. T. Phan et al.   LAB ON A CHIP

Organs-on-Chips with combined multi-electrode array and transepithelial electrical resistance measurement capabilities

(2017) Ben M. Maoz et al.   LAB ON A CHIP

Organoid cystogenesis reveals a critical role of microenvironment in human polycystic kidney disease

(2017) Nelly M. Cruz et al.   NATURE MATERIALS

Functional screening in human cardiac organoids reveals a metabolic mechanism for cardiomyocyte cell cycle arrest

(2017) Richard J. Mills et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Multisensor-integrated organs-on-chips platform for automated and continual in situ monitoring of organoid behaviors

(2017) Yu Shrike Zhang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A human brain microphysiological system derived from induced pluripotent stem cells to study neurological diseases and toxicity

(2017) David Pamies   ALTEX-Alternatives to Animal Experimentation

Matrix degradability controls multicellularity of 3D cell migration

(2017) Britta Trappmann et al.   Nature Communications

A microfluidic culture model of the human reproductive tract and 28-day menstrual cycle

(2017) Shuo Xiao et al.   Nature Communications

A Tissue Engineered Blood Vessel Model of Hutchinson-Gilford Progeria Syndrome Using Human iPSC-derived Smooth Muscle Cells

(2017) Leigh Atchison et al.   Scientific Reports

Placental Drug Transport-on-a-Chip: A Microengineered In Vitro Model of Transporter-Mediated Drug Efflux in the Human Placental Barrier

(2017) Cassidy Blundell et al.   Advanced Healthcare Materials

Human Organ Chip Models Recapitulate Orthotopic Lung Cancer Growth, Therapeutic Responses, and Tumor Dormancy In Vitro

(2017) Bryan A. Hassell et al.   Cell Reports

Synthesis of Microgel Sensors for Spatial and Temporal Monitoring of Protease Activity

(2017) Della S. Shin et al.   ACS Biomaterials-Science & Engineering

Synthesis of Microgel Sensors for Spatial and Temporal Monitoring of Protease Activity

(2017) Della S. Shin et al.   ACS Biomaterials Science & Engineering

Mature induced-pluripotent-stem-cell-derived human podocytes reconstitute kidney glomerular-capillary-wall function on a chip

(2017) Samira Musah et al.   Nature Biomedical Engineering

Multi-tissue interactions in an integrated three-tissue organ-on-a-chip platform

(2017) Aleksander Skardal et al.   Scientific Reports

Physiologically based kinetic modeling of the bioactivation of myristicin

(2016) Amer J. Al-Malahmeh et al.   ARCHIVES OF TOXICOLOGY

Mechanistic evaluation of primary human hepatocyte culture using global proteomic analysis reveals a selective dedifferentiation profile

(2016) James A. Heslop et al.   ARCHIVES OF TOXICOLOGY

Bioprinting 3D microfibrous scaffolds for engineering endothelialized myocardium and heart-on-a-chip

(2016) Yu Shrike Zhang et al.   BIOMATERIALS

Transitions from mono- to co- to tri-culture uniquely affect gene expression in breast cancer, stromal, and immune compartments

(2016) Mary C. Regier et al.   BIOMEDICAL MICRODEVICES

Macrophage-Secreted TNFα and TGFβ1 Influence Migration Speed and Persistence of Cancer Cells in 3D Tissue Culture via Independent Pathways

(2016) Ran Li et al.   CANCER RESEARCH

Acute pergolide exposure stiffens engineered valve interstitial cell tissues and reduces contractility in vitro

(2016) Andrew K. Capulli et al.   CARDIOVASCULAR PATHOLOGY

Reverse Engineering Human Pathophysiology with Organs-on-Chips

(2016) Donald E. Ingber   CELL

Organoid-on-a-chip and body-on-a-chip systems for drug screening and disease modeling

(2016) Aleksander Skardal et al.   DRUG DISCOVERY TODAY

Metabolic consequences of inflammatory disruption of the blood-brain barrier in an organ-on-chip model of the human neurovascular unit

(2016) Jacquelyn A. Brown et al.   Journal of Neuroinflammation

Human iPS Cell-Derived Neurons Uncover the Impact of Increased Ras Signaling in Costello Syndrome

(2016) G. E. Rooney et al.   JOURNAL OF NEUROSCIENCE

Development of a microphysiological model of human kidney proximal tubule function

(2016) Elijah J. Weber et al.   KIDNEY INTERNATIONAL

Beating heart on a chip: a novel microfluidic platform to generate functional 3D cardiac microtissues

(2016) Anna Marsano et al.   LAB ON A CHIP

Modular, pumpless body-on-a-chip platform for the co-culture of GI tract epithelium and 3D primary liver tissue

(2016) Mandy B. Esch et al.   LAB ON A CHIP

Instrumented cardiac microphysiological devices via multimaterial three-dimensional printing

(2016) Johan U. Lind et al.   NATURE MATERIALS

Engineered human pluripotent-stem-cell-derived intestinal tissues with a functional enteric nervous system

(2016) Michael J Workman et al.   NATURE MEDICINE

A practical guide to hydrogels for cell culture

(2016) Steven R Caliari et al.   NATURE METHODS

A microfluidics-based in vitro model of the gastrointestinal human–microbe interface

(2016) Pranjul Shah et al.   Nature Communications

Tissue chips to aid drug development and modeling for rare diseases

(2016) Lucie A. Low et al.   Expert Opinion on Orphan Drugs

Matched-Comparative Modeling of Normal and Diseased Human Airway Responses Using a Microengineered Breathing Lung Chip

(2016) Kambez H. Benam et al.   Cell Systems

Facilitating the commercialization and use of organ platforms generated by the microphysiological systems (Tissue Chip) program through public–private partnerships

(2016) Christine A. Livingston et al.   Computational and Structural Biotechnology Journal

Angiotensin II Induced Cardiac Dysfunction on a Chip

(2016) Renita E. Horton et al.   PLoS One

Distinct Contributions of Astrocytes and Pericytes to Neuroinflammation Identified in a 3D Human Blood-Brain Barrier on a Chip

(2016) Anna Herland et al.   PLoS One

Multi-Organ toxicity demonstration in a functional human in vitro system composed of four organs

(2016) Carlota Oleaga et al.   Scientific Reports

3D microtumors in vitro supported by perfused vascular networks

(2016) Agua Sobrino et al.   Scientific Reports

Recreating blood-brain barrier physiology and structure on chip: A novel neurovascular microfluidic bioreactor

(2015) Jacquelyn A. Brown et al.   Biomicrofluidics

Vascularized and Complex Organ Buds from Diverse Tissues via Mesenchymal Cell-Driven Condensation

(2015) Takanori Takebe et al.   Cell Stem Cell

Fluorescent protein biosensors applied to microphysiological systems

(2015) Nina Senutovitch et al.   EXPERIMENTAL BIOLOGY AND MEDICINE

A human liver microphysiology platform for investigating physiology, drug safety, and disease models

(2015) Lawrence A Vernetti et al.   EXPERIMENTAL BIOLOGY AND MEDICINE

A four-organ-chip for interconnected long-term co-culture of human intestine, liver, skin and kidney equivalents

(2015) Ilka Maschmeyer et al.   LAB ON A CHIP

An analysis of the attrition of drug candidates from four major pharmaceutical companies

(2015) Michael J. Waring et al.   NATURE REVIEWS DRUG DISCOVERY

Human pluripotent stem cell-derived neural constructs for predicting neural toxicity

(2015) Michael P. Schwartz et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Titin mutations in iPS cells define sarcomere insufficiency as a cause of dilated cardiomyopathy

(2015) J. T. Hinson et al.   SCIENCE

Gene introduction into the mitochondria of Arabidopsis thaliana via peptide-based carriers

(2015) Jo-Ann Chuah et al.   Scientific Reports

The Multi-organ Chip - A Microfluidic Platform for Long-term Multi-tissue Coculture

(2015) Eva-Maria Materne et al.   Jove-Journal of Visualized Experiments

μOrgano: A Lego®-Like Plug & Play System for Modular Multi-Organ-Chips

(2015) Peter Loskill et al.   PLoS One

Human iPSC-based Cardiac Microphysiological System For Drug Screening Applications

(2015) Anurag Mathur et al.   Scientific Reports

Human airway musculature on a chip: an in vitro model of allergic asthmatic bronchoconstriction and bronchodilation

(2014) Alexander Peyton Nesmith et al.   LAB ON A CHIP

Clinical development success rates for investigational drugs

(2014) Michael Hay et al.   NATURE BIOTECHNOLOGY

Modeling the mitochondrial cardiomyopathy of Barth syndrome with induced pluripotent stem cell and heart-on-chip technologies

(2014) Gang Wang et al.   NATURE MEDICINE

Lessons learned from the fate of AstraZeneca's drug pipeline: a five-dimensional framework

(2014) David Cook et al.   NATURE REVIEWS DRUG DISCOVERY

Hepatocyte circadian clock controls acetaminophen bioactivation through NADPH-cytochrome P450 oxidoreductase

(2014) Brian P. Johnson et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Human 3D vascularized organotypic microfluidic assays to study breast cancer cell extravasation

(2014) Jessie S. Jeon et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Variation in diffusion of gases through PDMS due to plasma surface treatment and storage conditions

(2013) Dmitry A. Markov et al.   BIOMEDICAL MICRODEVICES

Reducing attrition in drug development: smart loading preclinical safety assessment

(2013) Ruth A. Roberts et al.   DRUG DISCOVERY TODAY

Engineering Challenges for Instrumenting and Controlling Integrated Organ-on-Chip Systems

(2013) J. P. Wikswo et al.   IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING

Enabling screening in 3D microenvironments: probing matrix and stromal effects on the morphology and proliferation of T47D breast carcinoma cells

(2013) Sara I. Montanez-Sauri et al.   Integrative Biology

Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment

(2013) Kyung-Jin Jang et al.   Integrative Biology

Gut-on-a-Chip microenvironment induces human intestinal cells to undergo villus differentiation

(2013) Hyun Jung Kim et al.   Integrative Biology

Microfluidic heart on a chip for higher throughput pharmacological studies

(2013) Ashutosh Agarwal et al.   LAB ON A CHIP

Scaling and systems biology for integrating multiple organs-on-a-chip

(2013) John P. Wikswo et al.   LAB ON A CHIP

Three-dimensional co-cultures of human endothelial cells and embryonic stem cell-derived pericytes inside a microfluidic device

(2013) Andries D. van der Meer et al.   LAB ON A CHIP

Engineering of functional, perfusable 3D microvascular networks on a chip

(2013) Sudong Kim et al.   LAB ON A CHIP

Biowire: a platform for maturation of human pluripotent stem cell–derived cardiomyocytes

(2013) Sara S Nunes et al.   NATURE METHODS

Flow induces epithelial-mesenchymal transition, cellular heterogeneity and biomarker modulation in 3D ovarian cancer nodules

(2013) I. Rizvi et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Biomimetic model to reconstitute angiogenic sprouting morphogenesis in vitro

(2013) D.-H. T. Nguyen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Genomic responses in mouse models poorly mimic human inflammatory diseases

(2013) Junhee Seok et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Effects of Biologic Scaffolds on Human Stem Cells and Implications for CNS Tissue Engineering

(2013) Peter M. Crapo et al.   TISSUE ENGINEERING PART A

Physical influences on stem cells

(2013) Gordana Vunjak-Novakovic   Stem Cell Research & Therapy

Characterization of primary human hepatocytes, HepG2 cells, and HepaRG cells at the mRNA level and CYP activity in response to inducers and their predictivity for the detection of human hepatotoxins

(2012) H. H. J. Gerets et al.   CELL BIOLOGY AND TOXICOLOGY

Human gut-on-a-chip inhabited by microbial flora that experiences intestinal peristalsis-like motions and flow

(2012) Hyun Jung Kim et al.   LAB ON A CHIP

Diagnosing the decline in pharmaceutical R&D efficiency

(2012) Jack W. Scannell et al.   NATURE REVIEWS DRUG DISCOVERY

Three-dimensional microfluidic model for tumor cell intravasation and endothelial barrier function

(2012) I. K. Zervantonakis et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Bubbles no more: in-plane trapping and removal of bubbles in microfluidic devices

(2011) Conrad Lochovsky et al.   LAB ON A CHIP

Oxygen plasma treatment for reducing hydrophobicity of a sealed polydimethylsiloxane microchannel

(2010) Say Hwa Tan et al.   Biomicrofluidics

Epigenetic memory in induced pluripotent stem cells

(2010) K. Kim et al.   NATURE

How to improve R&D productivity: the pharmaceutical industry's grand challenge

(2010) Steven M. Paul et al.   NATURE REVIEWS DRUG DISCOVERY

Circadian Integration of Metabolism and Energetics

(2010) J. Bass et al.   SCIENCE

Reconstituting Organ-Level Lung Functions on a Chip

(2010) D. Huh et al.   SCIENCE

Towards a human-on-chip: Culturing multiple cell types on a chip with compartmentalized microenvironments

(2009) Chi Zhang et al.   LAB ON A CHIP

Biological implications of polydimethylsiloxane-based microfluidic cell culture

(2009) Keil J. Regehr et al.   LAB ON A CHIP

The Design and Fabrication of Three-Chamber Microscale Cell Culture Analog Devices with Integrated Dissolved Oxygen Sensors

(2004) Aaron Sin et al.   BIOTECHNOLOGY PROGRESS