Progress and potential in organoid research

A Living Biobank of Breast Cancer Organoids Captures Disease Heterogeneity

(2018) Norman Sachs et al.   CELL

Tumor Evolution and Drug Response in Patient-Derived Organoid Models of Bladder Cancer

(2018) Suk Hyung Lee et al.   CELL

High-Throughput Screening Enhances Kidney Organoid Differentiation from Human Pluripotent Stem Cells and Enables Automated Multidimensional Phenotyping

(2018) Stefan M. Czerniecki et al.   Cell Stem Cell

Engineered Tissue Folding by Mechanical Compaction of the Mesenchyme

(2018) Alex J. Hughes et al.   DEVELOPMENTAL CELL

Blastocyst-like structures generated solely from stem cells

(2018) Nicolas C. Rivron et al.   NATURE

An in vivo model of functional and vascularized human brain organoids

(2018) Abed AlFatah Mansour et al.   NATURE BIOTECHNOLOGY

Generation of human vascularized brain organoids

(2018) Missy T. Pham et al.   NEUROREPORT

Patient-derived organoids model treatment response of metastatic gastrointestinal cancers

(2018) Georgios Vlachogiannis et al.   SCIENCE

Micropattern differentiation of mouse pluripotent stem cells recapitulates embryo regionalized cell fate patterning

(2018) Sophie M Morgani et al.   eLife

Self-organization of a human organizer by combined Wnt and Nodal signalling

(2018) I. Martyn et al.   NATURE

The labs growing human embryos for longer than ever before

(2018) Helen Shen   NATURE

Wnt/β-catenin promotes gastric fundus specification in mice and humans

(2017) Kyle W. McCracken et al.   NATURE

Cell diversity and network dynamics in photosensitive human brain organoids

(2017) Giorgia Quadrato et al.   NATURE

Guided self-organization and cortical plate formation in human brain organoids

(2017) Madeline A Lancaster et al.   NATURE BIOTECHNOLOGY

Synthetic hydrogels for human intestinal organoid generation and colonic wound repair

(2017) Ricardo Cruz-Acuña et al.   NATURE CELL BIOLOGY

Multisensor-integrated organs-on-chips platform for automated and continual in situ monitoring of organoid behaviors

(2017) Yu Shrike Zhang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Assembly of embryonic and extraembryonic stem cells to mimic embryogenesis in vitro

(2017) Sarah Ellys Harrison et al.   SCIENCE

iPSC-Derived Retina Transplants Improve Vision in rd1 End-Stage Retinal-Degeneration Mice

(2017) Michiko Mandai et al.   Stem Cell Reports

Photoreceptor Outer Segment-like Structures in Long-Term 3D Retinas from Human Pluripotent Stem Cells

(2017) Karl J. Wahlin et al.   Scientific Reports

In Situ Patterning of Microfluidic Networks in 3D Cell-Laden Hydrogels

(2016) Nathalie Brandenberg et al.   ADVANCED MATERIALS

Bioreactors for tissue engineering: An update

(2016) Junjie Zhao et al.   BIOCHEMICAL ENGINEERING JOURNAL

Bioprinting 3D microfibrous scaffolds for engineering endothelialized myocardium and heart-on-a-chip

(2016) Yu Shrike Zhang et al.   BIOMATERIALS

A Three-Dimensional Organoid Culture System Derived from Human Glioblastomas Recapitulates the Hypoxic Gradients and Cancer Stem Cell Heterogeneity of Tumors Found In Vivo

(2016) Christopher G. Hubert et al.   CANCER RESEARCH

Brain-Region-Specific Organoids Using Mini-bioreactors for Modeling ZIKV Exposure

(2016) Xuyu Qian et al.   CELL

Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors

(2016) Leonardo Morsut et al.   CELL

Modeling Development and Disease with Organoids

(2016) Hans Clevers   CELL

2D and 3D Stem Cell Models of Primate Cortical Development Identify Species-Specific Differences in Progenitor Behavior Contributing to Brain Size

(2016) Tomoki Otani et al.   Cell Stem Cell

Cystic Fibrosis Patients Benefit from Mini Guts

(2016) Angela Saini   Cell Stem Cell

Induction of Expandable Tissue-Specific Stem/Progenitor Cells through Transient Expression of YAP/TAZ

(2016) Tito Panciera et al.   Cell Stem Cell

Zika Virus Depletes Neural Progenitors in Human Cerebral Organoids through Activation of the Innate Immune Receptor TLR3

(2016) Jason Dang et al.   Cell Stem Cell

Engineering Stem Cell Organoids

(2016) Xiaolei Yin et al.   Cell Stem Cell

A Colorectal Tumor Organoid Library Demonstrates Progressive Loss of Niche Factor Requirements during Tumorigenesis

(2016) Masayuki Fujii et al.   Cell Stem Cell

Development-on-chip:in vitroneural tube patterning with a microfluidic device

(2016) Christopher J. Demers et al.   DEVELOPMENT

Derivation of a robust mouse mammary organoid system for studying tissue dynamics

(2016) Paul R. Jamieson et al.   DEVELOPMENT

Dishing out mini-brains: Current progress and future prospects in brain organoid research

(2016) Iva Kelava et al.   DEVELOPMENTAL BIOLOGY

A strategy for generating kidney organoids: Recapitulating the development in human pluripotent stem cells

(2016) Minoru Takasato et al.   DEVELOPMENTAL BIOLOGY

A Balance between Secreted Inhibitors and Edge Sensing Controls Gastruloid Self-Organization

(2016) Fred Etoc et al.   DEVELOPMENTAL CELL

Organoids: Modeling Development and the Stem Cell Niche in a Dish

(2016) Kai Kretzschmar et al.   DEVELOPMENTAL CELL

Organoids: A historical perspective of thinking in three dimensions

(2016) Marina Simian et al.   JOURNAL OF CELL BIOLOGY

Functional Characterization of Cholera Toxin Inhibitors Using Human Intestinal Organoids

(2016) Domenique D. Zomer-van Ommen et al.   JOURNAL OF MEDICINAL CHEMISTRY

Frizzled proteins are colonic epithelial receptors for C. difficile toxin B

(2016) Liang Tao et al.   NATURE

Visualization of a short-range Wnt gradient in the intestinal stem-cell niche

(2016) Henner F. Farin et al.   NATURE

The Brazilian Zika virus strain causes birth defects in experimental models

(2016) Fernanda R. Cugola et al.   NATURE

Erratum: Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis

(2016) Minoru Takasato et al.   NATURE

Designer matrices for intestinal stem cell and organoid culture

(2016) Nikolce Gjorevski et al.   NATURE

Resolving early mesoderm diversification through single-cell expression profiling

(2016) Antonio Scialdone et al.   NATURE

Self-organized amniogenesis by human pluripotent stem cells in a biomimetic implantation-like niche

(2016) Yue Shao et al.   NATURE MATERIALS

Engineered human pluripotent-stem-cell-derived intestinal tissues with a functional enteric nervous system

(2016) Michael J Workman et al.   NATURE MEDICINE

Culture and establishment of self-renewing human and mouse adult liver and pancreas 3D organoids and their genetic manipulation

(2016) Laura Broutier et al.   Nature Protocols

Organoid culture systems for prostate epithelial and cancer tissue

(2016) Jarno Drost et al.   Nature Protocols

Neural tube morphogenesis in synthetic 3D microenvironments

(2016) Adrian Ranga et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Zika virus impairs growth in human neurospheres and brain organoids

(2016) P. P. Garcez et al.   SCIENCE

Characterizing responses to CFTR-modulating drugs using rectal organoids derived from subjects with cystic fibrosis

(2016) Johanna F. Dekkers et al.   Science Translational Medicine

Wnt and Neuregulin1/ErbB signalling extends 3D culture of hormone responsive mammary organoids

(2016) Thierry Jardé et al.   Nature Communications

Rapid Induction of Cerebral Organoids From Human Induced Pluripotent Stem Cells Using a Chemically Defined Hydrogel and Defined Cell Culture Medium

(2016) Beth A. Lindborg et al.   Stem Cells Translational Medicine

Long-Term In Vitro Expansion of Salivary Gland Stem Cells Driven by Wnt Signals

(2016) Martti Maimets et al.   Stem Cell Reports

Organoids and the genetically encoded self-assembly of embryonic stem cells

(2015) David A. Turner et al.   BIOESSAYS

Versatile fabrication of vascularizable scaffolds for large tissue engineering in bioreactor

(2015) Alessandro Tocchio et al.   BIOMATERIALS

Long-Term Culture of Genome-Stable Bipotent Stem Cells from Adult Human Liver

(2015) Meritxell Huch et al.   CELL

FOXG1-Dependent Dysregulation of GABA/Glutamate Neuron Differentiation in Autism Spectrum Disorders

(2015) Jessica Mariani et al.   CELL

Prospective Derivation of a Living Organoid Biobank of Colorectal Cancer Patients

(2015) Marc van de Wetering et al.   CELL

Organoid Models of Human and Mouse Ductal Pancreatic Cancer

(2015) Sylvia F. Boj et al.   CELL

Regeneration of Thyroid Function by Transplantation of Differentiated Pluripotent Stem Cells

(2015) Anita A. Kurmann et al.   Cell Stem Cell

Positional information and reaction-diffusion: two big ideas in developmental biology combine

(2015) J. B. A. Green et al.   DEVELOPMENT

Quantification of regenerative potential in primary human mammary epithelial cells

(2015) J. R. Linnemann et al.   DEVELOPMENT

In Vitro Expansion of Human Gastric Epithelial Stem Cells and Their Responses to Bacterial Infection

(2015) Sina Bartfeld et al.   GASTROENTEROLOGY

Interaction of Salmonella enterica Serovar Typhimurium with Intestinal Organoids Derived from Human Induced Pluripotent Stem Cells

(2015) Jessica L. Forbester et al.   INFECTION AND IMMUNITY

Nephron organoids derived from human pluripotent stem cells model kidney development and injury

(2015) Ryuji Morizane et al.   NATURE BIOTECHNOLOGY

Generation of stomach tissue from mouse embryonic stem cells

(2015) Taka-aki K. Noguchi et al.   NATURE CELL BIOLOGY

What if stem cells turn into embryos in a dish?

(2015) Martin F Pera et al.   NATURE METHODS

Programmed synthesis of three-dimensional tissues

(2015) Michael E Todhunter et al.   NATURE METHODS

Transplantation of human embryonic stem cell-derived retinal tissue in two primate models of retinal degeneration

(2015) Hiroshi Shirai et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Plasticity of Hopx+ type I alveolar cells to regenerate type II cells in the lung

(2015) Rajan Jain et al.   Nature Communications

Generation of functional hippocampal neurons from self-organizing human embryonic stem cell-derived dorsomedial telencephalic tissue

(2015) Hideya Sakaguchi et al.   Nature Communications

Generation of a ciliary margin-like stem cell niche from self-organizing human retinal tissue

(2015) Atsushi Kuwahara et al.   Nature Communications

Modelling kidney disease with CRISPR-mutant kidney organoids derived from human pluripotent epiblast spheroids

(2015) Benjamin S. Freedman et al.   Nature Communications

Self-Organization of Polarized Cerebellar Tissue in 3D Culture of Human Pluripotent Stem Cells

(2015) Keiko Muguruma et al.   Cell Reports

3D Biofabrication Strategies for Tissue Engineering and Regenerative Medicine

(2014) Piyush Bajaj et al.   Annual Review of Biomedical Engineering

Organoid Cultures Derived from Patients with Advanced Prostate Cancer

(2014) Dong Gao et al.   CELL

YAP/TAZ Incorporation in the β-Catenin Destruction Complex Orchestrates the Wnt Response

(2014) Luca Azzolin et al.   CELL

Genome-wide RNA Tomography in the Zebrafish Embryo

(2014) Jan Philipp Junker et al.   CELL

Identification of Multipotent Luminal Progenitor Cells in Human Prostate Organoid Cultures

(2014) Wouter R. Karthaus et al.   CELL

Symmetry breaking, germ layer specification and axial organisation in aggregates of mouse embryonic stem cells

(2014) S. C. van den Brink et al.   DEVELOPMENT

Small intestinal stem cell identity is maintained with functional Paneth cells in heterotopically grafted epithelium onto the colon

(2014) Masayoshi Fukuda et al.   GENES & DEVELOPMENT

Persistence and Toxin Production by Clostridium difficile within Human Intestinal Organoids Result in Disruption of Epithelial Paracellular Barrier Function

(2014) Jhansi L. Leslie et al.   INFECTION AND IMMUNITY

Paneth cell extrusion and release of antimicrobial products is directly controlled by immune cell–derived IFN-γ

(2014) Henner F. Farin et al.   JOURNAL OF EXPERIMENTAL MEDICINE

Engineering interconnected 3D vascular networks in hydrogels using molded sodium alginate lattice as the sacrificial template

(2014) Xue-Ying Wang et al.   LAB ON A CHIP

A small intestinal organoid model of non-invasive enteric pathogen–epithelial cell interactions

(2014) S S Wilson et al.   Mucosal Immunology

Modelling human development and disease in pluripotent stem-cell-derived gastric organoids

(2014) Kyle W. McCracken et al.   NATURE

Single luminal epithelial progenitors can generate prostate organoids in culture

(2014) Chee Wai Chua et al.   NATURE CELL BIOLOGY

An in vivo model of human small intestine using pluripotent stem cells

(2014) Carey L Watson et al.   NATURE MEDICINE

A method to recapitulate early embryonic spatial patterning in human embryonic stem cells

(2014) Aryeh Warmflash et al.   NATURE METHODS

Generation of cerebral organoids from human pluripotent stem cells

(2014) Madeline A Lancaster et al.   Nature Protocols

IL-6/STAT3 promotes regeneration of airway ciliated cells from basal stem cells

(2014) T. Tadokoro et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Single Lgr5- or Lgr6-expressing taste stem/progenitor cells generate taste bud cells ex vivo

(2014) Wenwen Ren et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Organogenesis in a dish: Modeling development and disease using organoid technologies

(2014) M. A. Lancaster et al.   SCIENCE

Cellular Heterogeneity in the Mouse Esophagus Implicates the Presence of a Nonquiescent Epithelial Stem Cell Population

(2014) Aaron D. DeWard et al.   Cell Reports

Purification and Ex Vivo Expansion of Fully Functional Salivary Gland Stem Cells

(2014) Lalitha S.Y. Nanduri et al.   Stem Cell Reports

3D Reconstitution of the Patterned Neural Tube from Embryonic Stem Cells

(2014) Andrea Meinhardt et al.   Stem Cell Reports

Transplantation of Embryonic and Induced Pluripotent Stem Cell-Derived 3D Retinal Sheets into Retinal Degenerative Mice

(2014) Juthaporn Assawachananont et al.   Stem Cell Reports

Defined Polymer-Peptide Conjugates to Form Cell-Instructive starPEG-Heparin Matrices In Situ

(2013) Mikhail V. Tsurkan et al.   ADVANCED MATERIALS

Differentiated Troy+ Chief Cells Act as Reserve Stem Cells to Generate All Lineages of the Stomach Epithelium

(2013) Daniel E. Stange et al.   CELL

Transplantation of Expanded Fetal Intestinal Progenitors Contributes to Colon Regeneration after Injury

(2013) Robert P. Fordham et al.   Cell Stem Cell

Functional Repair of CFTR by CRISPR/Cas9 in Intestinal Stem Cell Organoids of Cystic Fibrosis Patients

(2013) Gerald Schwank et al.   Cell Stem Cell

Human iPSC-Based Modeling of Late-Onset Disease via Progerin-Induced Aging

(2013) Justine D. Miller et al.   Cell Stem Cell

Redefining the In Vivo Origin of Metanephric Nephron Progenitors Enables Generation of Complex Kidney Structures from Pluripotent Stem Cells

(2013) Atsuhiro Taguchi et al.   Cell Stem Cell

Artificial three-dimensional niches deconstruct pancreas development in vitro

(2013) C. Greggio et al.   DEVELOPMENT

Unlimited in vitro expansion of adult bi-potent pancreas progenitors through the Lgr5/R-spondin axis

(2013) Meritxell Huch et al.   EMBO JOURNAL

Human Primary Intestinal Epithelial Cells as an ImprovedIn VitroModel for Cryptosporidium parvum Infection

(2013) Alejandro Castellanos-Gonzalez et al.   INFECTION AND IMMUNITY

Type 2 alveolar cells are stem cells in adult lung

(2013) Christina E. Barkauskas et al.   JOURNAL OF CLINICAL INVESTIGATION

Cytosystems dynamics in self-organization of tissue architecture

(2013) Yoshiki Sasai   NATURE

In vitro expansion of single Lgr5+ liver stem cells induced by Wnt-driven regeneration

(2013) Meritxell Huch et al.   NATURE

Vascularized and functional human liver from an iPSC-derived organ bud transplant

(2013) Takanori Takebe et al.   NATURE

Cerebral organoids model human brain development and microcephaly

(2013) Madeline A. Lancaster et al.   NATURE

Efficient generation of lung and airway epithelial cells from human pluripotent stem cells

(2013) Sarah X L Huang et al.   NATURE BIOTECHNOLOGY

Directed differentiation of human pluripotent cells to ureteric bud kidney progenitor-like cells

(2013) Yun Xia et al.   NATURE CELL BIOLOGY

Directing human embryonic stem cell differentiation towards a renal lineage generates a self-organizing kidney

(2013) M. Takasato et al.   NATURE CELL BIOLOGY

In situ cell manipulation through enzymatic hydrogel photopatterning

(2013) Katarzyna A. Mosiewicz et al.   NATURE MATERIALS

A functional CFTR assay using primary cystic fibrosis intestinal organoids

(2013) Johanna F Dekkers et al.   NATURE MEDICINE

Growing Self-Organizing Mini-Guts from a Single Intestinal Stem Cell: Mechanism and Applications

(2013) T. Sato et al.   SCIENCE

Generation of Multipotent Foregut Stem Cells from Human Pluripotent Stem Cells

(2013) Nicholas R.F. Hannan et al.   Stem Cell Reports

Self-Formation of Optic Cups and Storable Stratified Neural Retina from Human ESCs

(2012) Tokushige Nakano et al.   Cell Stem Cell

Bistability, Bifurcations, and Waddington's Epigenetic Landscape

(2012) James E. Ferrell   CURRENT BIOLOGY

Directed differentiation of human pluripotent stem cells into mature airway epithelia expressing functional CFTR protein

(2012) Amy P Wong et al.   NATURE BIOTECHNOLOGY

Rapid casting of patterned vascular networks for perfusable engineered three-dimensional tissues

(2012) Jordan S. Miller et al.   NATURE MATERIALS

Functional engraftment of colon epithelium expanded in vitro from a single adult Lgr5+ stem cell

(2012) Shiro Yui et al.   NATURE MEDICINE

Stem Cell-Derived Human Intestinal Organoids as an Infection Model for Rotaviruses

(2012) S. R. Finkbeiner et al.   mBio

Maleimide Cross-Linked Bioactive PEG Hydrogel Exhibits Improved Reaction Kinetics and Cross-Linking for Cell Encapsulation and In Situ Delivery

(2011) Edward A. Phelps et al.   ADVANCED MATERIALS

Long-term Expansion of Epithelial Organoids From Human Colon, Adenoma, Adenocarcinoma, and Barrett's Epithelium

(2011) Toshiro Sato et al.   GASTROENTEROLOGY

Self-formation of functional adenohypophysis in three-dimensional culture

(2011) Hidetaka Suga et al.   NATURE

Self-organizing optic-cup morphogenesis in three-dimensional culture

(2011) Mototsugu Eiraku et al.   NATURE

Spatially controlled simultaneous patterning of multiple growth factors in three-dimensional hydrogels

(2011) Ryan G. Wylie et al.   NATURE MATERIALS

Isolation and in vitro expansion of human colonic stem cells

(2011) Peter Jung et al.   NATURE MEDICINE

Sox9 expression marks a subset of CD24-expressing small intestine epithelial stem cells that form organoids in vitro

(2010) Adam D. Gracz et al.   AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY

Sorting mouse jejunal epithelial cells with CD24 yields a population with characteristics of intestinal stem cells

(2010) Richard J. von Furstenberg et al.   AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY

Intestinal Crypt Homeostasis Results from Neutral Competition between Symmetrically Dividing Lgr5 Stem Cells

(2010) Hugo J. Snippert et al.   CELL

Lgr5+ve Stem Cells Drive Self-Renewal in the Stomach and Build Long-Lived Gastric Units In Vitro

(2010) Nick Barker et al.   Cell Stem Cell

Paneth cells constitute the niche for Lgr5 stem cells in intestinal crypts

(2010) Toshiro Sato et al.   NATURE

Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro

(2010) Jason R. Spence et al.   NATURE

Cancer stem cells from colorectal cancer-derived cell lines

(2010) Trevor M. Yeung et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Homogeneous and organized differentiation within embryoid bodies induced by microsphere-mediated delivery of small molecules

(2009) Richard L. Carpenedo et al.   BIOMATERIALS

Towards a human-on-chip: Culturing multiple cell types on a chip with compartmentalized microenvironments

(2009) Chi Zhang et al.   LAB ON A CHIP

Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche

(2009) Toshiro Sato et al.   NATURE

Sequential click reactions for synthesizing and patterning three-dimensional cell microenvironments

(2009) Cole A. DeForest et al.   NATURE MATERIALS

Sustained in vitro intestinal epithelial culture within a Wnt-dependent stem cell niche

(2009) Akifumi Ootani et al.   NATURE MEDICINE

Self-organization of engineered epithelial tubules by differential cellular motility

(2009) H. Mori et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Basal cells as stem cells of the mouse trachea and human airway epithelium

(2009) J. R. Rock et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Isolation and characterization of human salivary gland cells for stem cell transplantation to reduce radiation-induced hyposalivation

(2009) Jielin Feng et al.   RADIOTHERAPY AND ONCOLOGY

Photodegradable Hydrogels for Dynamic Tuning of Physical and Chemical Properties

(2009) April M. Kloxin et al.   SCIENCE

Differentiation of Embryonic Stem Cells to Clinically Relevant Populations: Lessons from Embryonic Development

(2008) Charles E. Murry et al.   CELL

Self-Organized Formation of Polarized Cortical Tissues from ESCs and Its Active Manipulation by Extrinsic Signals

(2008) Mototsugu Eiraku et al.   Cell Stem Cell

Biomolecular gradients in cell culture systems

(2007) Thomas M. Keenan et al.   LAB ON A CHIP