Fluorescent protein biosensors applied to microphysiological systems

From fluorescence polarization to Quenchbody: Recent progress in fluorescent reagentless biosensors based on antibody and other binding proteins

(2014) Hiroshi Ueda et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Development and Applications of CRISPR-Cas9 for Genome Engineering

(2014) Patrick D. Hsu et al.   CELL

Live-cell reporters for fluorescence imaging

(2014) Ivan R Corrêa   CURRENT OPINION IN CHEMICAL BIOLOGY

The relevance and potential roles of microphysiological systems in biology and medicine

(2014) John P Wikswo   EXPERIMENTAL BIOLOGY AND MEDICINE

Tissue-engineered microenvironment systems for modeling human vasculature

(2014) Anna Tourovskaia et al.   EXPERIMENTAL BIOLOGY AND MEDICINE

In vitro platforms for evaluating liver toxicity

(2014) Shyam Sundhar Bale et al.   EXPERIMENTAL BIOLOGY AND MEDICINE

CRISPR-Cas systems for editing, regulating and targeting genomes

(2014) Jeffry D Sander et al.   NATURE BIOTECHNOLOGY

Microfluidic organs-on-chips

(2014) Sangeeta N Bhatia et al.   NATURE BIOTECHNOLOGY

Tracking single molecules at work in living cells

(2014) Akihiro Kusumi et al.   Nature Chemical Biology

Development of a doxycycline-inducible lentiviral plasmid with an instant regulatory feature

(2014) Tian Yang et al.   PLASMID

Identifying and Quantifying Heterogeneity in High Content Analysis: Application of Heterogeneity Indices to Drug Discovery

(2014) Albert H. Gough et al.   PLoS One

Quantitative measurement of intracellular protein dynamics using photobleaching or photoactivation of fluorescent proteins

(2014) Tomoki Matsuda et al.   Microscopy

Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity

(2013) F. Ann Ran et al.   CELL

Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases

(2013) S. W. Cho et al.   GENOME RESEARCH

Respective Contribution of Mitochondrial Superoxide and pH to Mitochondria-targeted Circularly Permuted Yellow Fluorescent Protein (mt-cpYFP) Flash Activity

(2013) Lan Wei-LaPierre et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Scaling and systems biology for integrating multiple organs-on-a-chip

(2013) John P. Wikswo et al.   LAB ON A CHIP

Advances in multiphoton microscopy technology

(2013) Erich E. Hoover et al.   Nature Photonics

Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis

(2013) Z. Hou et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells

(2013) Ophir Shalem et al.   SCIENCE

A Versatile Toolkit to Produce Sensitive FRET Biosensors to Visualize Signaling in Time and Space

(2013) R. D. Fritz et al.   Science Signaling

ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering

(2013) Thomas Gaj et al.   TRENDS IN BIOTECHNOLOGY

Towards a three-dimensional microfluidic liver platform for predicting drug efficacy and toxicity in humans

(2013) Abhinav Bhushan et al.   Stem Cell Research & Therapy

Accurate Prediction of Drug-Induced Liver Injury Using Stem Cell-Derived Populations

(2013) Dagmara Szkolnicka et al.   Stem Cells Translational Medicine

Genetically encoded calcium indicators for multi-color neural activity imaging and combination with optogenetics

(2013) Jasper Akerboom et al.   Frontiers in Molecular Neuroscience

In Vivo Application of Optogenetics for Neural Circuit Analysis

(2012) Xue Han   ACS Chemical Neuroscience

Single-molecule monitoring in living cells by use of fluorescence microscopy

(2012) Wangxi Luo et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Genetically Encoded pH Sensor for Tracking Surface Proteins through Endocytosis

(2012) Anmol Grover et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Analysis of Kinetics of Dihydroethidium Fluorescence with Superoxide Using Xanthine Oxidase and Hypoxanthine Assay

(2012) Juan Chen et al.   ANNALS OF BIOMEDICAL ENGINEERING

Lentiviral vectors: basic to translational

(2012) Toshie Sakuma et al.   BIOCHEMICAL JOURNAL

FRET microscopy in the living cell: Different approaches, strengths and weaknesses

(2012) Sergi Padilla-Parra et al.   BIOESSAYS

Role of immune reactions in drug-induced liver injury (DILI)

(2012) Cynthia Ju et al.   DRUG METABOLISM REVIEWS

Mitochondria-targeted cpYFP: pH or superoxide sensor?

(2012) Emilie Quatresous et al.   JOURNAL OF GENERAL PHYSIOLOGY

Optimization of a GCaMP Calcium Indicator for Neural Activity Imaging

(2012) J. Akerboom et al.   JOURNAL OF NEUROSCIENCE

Chemical biology-based approaches on fluorescent labeling of proteins in live cells

(2012) Deokho Jung et al.   Molecular BioSystems

Advanced Fluorescence Microscopy Techniques—FRAP, FLIP, FLAP, FRET and FLIM

(2012) Hellen C. Ishikawa-Ankerhold et al.   MOLECULES

A Sensitive Membrane-Targeted Biosensor for Monitoring Changes in Intracellular Chloride in Neuronal Processes

(2012) Spencer D. Watts et al.   PLoS One

Modeling hepatitis C virus infection using human induced pluripotent stem cells

(2012) R. E. Schwartz et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Real-time concurrent monitoring of apoptosis, cytosolic calcium, and mitochondria permeability transition for hypermulticolor high-content screening of drug-induced mitochondrial dysfunction-mediated hepatotoxicity

(2012) Jae-Ah Kim et al.   TOXICOLOGY LETTERS

Development of Probes for Cellular Functions Using Fluorescent Proteins and Fluorescence Resonance Energy Transfer

(2011) Atsushi Miyawaki   Annual Review of Biochemistry

Changes in mitochondrial redox state, membrane potential and calcium precede mitochondrial dysfunction in doxorubicin-induced cell death

(2011) Andrey V. Kuznetsov et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH

Mitochondrial membrane potential probes and the proton gradient: a practical usage guide

(2011) Seth W. Perry et al.   BIOTECHNIQUES

Generation of Isogenic Pluripotent Stem Cells Differing Exclusively at Two Early Onset Parkinson Point Mutations

(2011) Frank Soldner et al.   CELL

Recent advances in dynamic intravital multi-photon microscopy

(2011) Raluca A. Niesner et al.   CYTOMETRY PART A

Genetic engineering of human pluripotent cells using TALE nucleases

(2011) Dirk Hockemeyer et al.   NATURE BIOTECHNOLOGY

Fluorescence anisotropy: from single molecules to live cells

(2010) Claudiu C. Gradinaru et al.   ANALYST

Mammalian cell transfection: the present and the future

(2010) Tae Kyung Kim et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Cyto-Tracers™: Novel lentiviral-based molecular imaging tools

(2010) Maggie Fong et al.   BIOTECHNIQUES

Cell-Cycle Markers and Biosensors

(2010) Laetitia Kurzawa et al.   CHEMBIOCHEM

Fluorescence anisotropy and resonance energy transfer: powerful tools for measuring real time protein dynamics in a physiological environment

(2010) Christopher M Yengo et al.   CURRENT OPINION IN PHARMACOLOGY

Optogenetic monitoring of membrane potentials

(2010) Hiroki Mutoh et al.   EXPERIMENTAL PHYSIOLOGY

Fluorescent proteins at a glance

(2010) G.-J. Kremers et al.   JOURNAL OF CELL SCIENCE

Bioenergetic Metabolites Regulate Base Excision Repair-Dependent Cell Death in Response to DNA Damage

(2010) J.-b. Tang et al.   MOLECULAR CANCER RESEARCH

High-performance genetically targetable optical neural silencing by light-driven proton pumps

(2010) Brian Y. Chow et al.   NATURE

Ultrafast optogenetic control

(2010) Lisa A Gunaydin et al.   NATURE NEUROSCIENCE

The Structure of Ca2+ Sensor Case16 Reveals the Mechanism of Reaction to Low Ca2+ Concentrations

(2010) Lukas Leder et al.   SENSORS

Practical and reliable FRET/FLIM pair of fluorescent proteins

(2009) Dmitry Shcherbo et al.   BMC BIOTECHNOLOGY

Fluorescent Biosensors of Intracellular Targets from Genetically Encoded Reporters to Modular Polypeptide Probes

(2009) May C. Morris   CELL BIOCHEMISTRY AND BIOPHYSICS

The photochemistry of fluorescent proteins: implications for their biological applications

(2009) Harriet E. Seward et al.   CHEMICAL SOCIETY REVIEWS

The structure and function of fluorescent proteins

(2009) Vedangi Sample et al.   CHEMICAL SOCIETY REVIEWS

Genetically encoded biosensors based on engineered fluorescent proteins

(2009) Wolf B. Frommer et al.   CHEMICAL SOCIETY REVIEWS

Photoactivation mechanism of PAmCherry based on crystal structures of the protein in the dark and fluorescent states

(2009) Fedor V. Subach et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

HaloTag: A Novel Protein Labeling Technology for Cell Imaging and Protein Analysis

(2008) Georgyi V. Los et al.   ACS Chemical Biology

Visualizing Spatiotemporal Dynamics of Multicellular Cell-Cycle Progression

(2008) Asako Sakaue-Sawano et al.   CELL

Conversion of Red Fluorescent Protein into a Bright Blue Probe

(2008) Oksana M. Subach et al.   CHEMISTRY & BIOLOGY

Multiphoton microscopy and fluorescence lifetime imaging microscopy (FLIM) to monitor metastasis and the tumor microenvironment

(2008) Paolo P. Provenzano et al.   CLINICAL & EXPERIMENTAL METASTASIS

Cell-surface protein-protein interaction analysis with time-resolved FRET and snap-tag technologies: application to GPCR oligomerization

(2008) Damien Maurel et al.   NATURE METHODS

Genetic modification of stem cells for transplantation

(2007) M. Ian Phillips et al.   ADVANCED DRUG DELIVERY REVIEWS