Advancements in stem cell-derived hepatocyte-like cell models for hepatotoxicity testing

Cellulose Nanofibril Hydrogel Promotes Hepatic Differentiation of Human Liver Organoids

(2020) Melanie Krüger et al.   Advanced Healthcare Materials

Advanced Biomaterials and Processing Methods for Liver Regeneration: State‐of‐the‐Art and Future Trends

(2020) Alain da Silva Morais et al.   Advanced Healthcare Materials

Human‐on‐Leaf‐Chip: A Biomimetic Vascular System Integrated with Chamber‐Specific Organs

(2020) Mao Mao et al.   Small

Oxygen plasma substrate and specific nanopattern promote early differentiation of HepaRG progenitors

(2020) Katie Morgan et al.   TISSUE ENGINEERING PART A

Amino acid levels determine metabolism and CYP450 function of hepatocytes and hepatoma cell lines

(2020) Ruben Boon et al.   Nature Communications

Repeated dose multi-drug testing using a microfluidic chip-based coculture of human liver and kidney proximal tubules equivalents

(2020) Ni Lin et al.   Scientific Reports

Human multi-organ chip co-culture of bronchial lung culture and liver spheroids for substance exposure studies

(2020) Katharina Schimek et al.   Scientific Reports

Synthetic alternatives to Matrigel

(2020) Elizabeth A. Aisenbrey et al.   Nature Reviews Materials

Microfluidic organ-on-a-chip models of human liver tissue

(2020) Ehsanollah Moradi et al.   Acta Biomaterialia

Generation of hepato-biliary organoids from human induced pluripotent stem cells

(2019) Fenfang Wu et al.   JOURNAL OF HEPATOLOGY

EASL Clinical Practice Guidelines: Drug-induced liver injury

(2019) Raúl Andrade et al.   JOURNAL OF HEPATOLOGY

Incidence and Etiology of Drug-Induced Liver Injury in Mainland China

(2019) Tao Shen et al.   GASTROENTEROLOGY

Cleavable cellulosic sponge for functional hepatic cell culture and retrieval

(2019) Min Sun et al.   BIOMATERIALS

Endoplasmic Reticulum Stress-induced Upregulation of STARD1 Promotes Acetaminophen-induced Acute Liver Failure

(2019) Sandra Torres et al.   GASTROENTEROLOGY

Cell-Based Assays on Microfluidics for Drug Screening

(2019) Xiaoyan Liu et al.   ACS Sensors

3-D Culture and Endothelial Cells Improve Maturity of Human Pluripotent Stem Cell-Derived Hepatocytes

(2019) Hamisha Ardalani et al.   Acta Biomaterialia

Advances in Hydrogels in Organoids and Organs‐on‐a‐Chip

(2019) Haitao Liu et al.   ADVANCED MATERIALS

Modeling Steatohepatitis in Humans with Pluripotent Stem Cell-Derived Organoids

(2019) Rie Ouchi et al.   Cell Metabolism

Generation of expandable human pluripotent stem cell-derived hepatocyte-like liver organoids

(2019) Seon Ju Mun et al.   JOURNAL OF HEPATOLOGY

Generation of fully functional hepatocyte-like organoids from human induced pluripotent stem cells mixed with Endothelial Cells

(2019) Giuseppe Pettinato et al.   Scientific Reports

A DMSO-free hepatocyte maturation medium accelerates hepatic differentiation of HepaRG cells in vitro

(2019) Zhen-Yu Wang et al.   BIOMEDICINE & PHARMACOTHERAPY

Decellularized amniotic membrane Scaffolds improve differentiation of iPSCs to functional hepatocyte‐like cells

(2019) Mohammad Foad Abazari et al.   JOURNAL OF CELLULAR BIOCHEMISTRY

Adult and iPS-derived non-parenchymal cells regulate liver organoid development through differential modulation of Wnt and TGF-β

(2019) Ernesto Goulart et al.   Stem Cell Research & Therapy

Enhanced hepatic differentiation in the subpopulation of human amniotic stem cells under 3D multicellular microenvironment

(2019) Kinji Furuya et al.   World Journal of Stem Cells

Human ESC-derived expandable hepatic organoids enable therapeutic liver repopulation and pathophysiological modeling of alcoholic liver injury

(2019) Shuyong Wang et al.   CELL RESEARCH

Large‐scale Production of LGR5‐positive Bipotential Human Liver Stem Cells

(2019) Kerstin Schneeberger et al.   HEPATOLOGY

Robust, Long-Term Culture of Endoderm-Derived Hepatic Organoids for Disease Modeling

(2019) Soheil Akbari et al.   Stem Cell Reports

Epidemiology, Predisposing Factors, and Outcomes of Drug-Induced Liver Injury

(2019) Einar S. Björnsson   Clinics in Liver Disease

Metabolomic profiling during the differentiation of human induced pluripotent stem cells into hepatocyte-like cells

(2019) Rachid Jellali et al.   DIFFERENTIATION

A Hepatic Scaffold from Decellularized Liver Tissue: Food for Thought

(2019) Stefania Croce et al.   Biomolecules

Critical differences in drug metabolic properties of human hepatic cellular models, including primary human hepatocytes, stem cell derived hepatocytes, and hepatoma cell lines

(2018) Alexander J. Kvist et al.   BIOCHEMICAL PHARMACOLOGY

Enrichment of high-functioning human iPS cell-derived hepatocyte-like cells for pharmaceutical research

(2018) Kazuo Takayama et al.   BIOMATERIALS

Differentiation of hepatocyte-like cells from human pluripotent stem cells using small molecules

(2018) Faizal Z. Asumda et al.   DIFFERENTIATION

Chimeric mice with humanized liver: Application in drug metabolism and pharmacokinetics studies for drug discovery

(2018) Yoichi Naritomi et al.   Drug Metabolism and Pharmacokinetics

Three-dimensional liver-derived extracellular matrix hydrogel promotes liver organoids function

(2018) Mona Saheli et al.   JOURNAL OF CELLULAR BIOCHEMISTRY

Fabrication of agarose concave petridish for 3D-culture microarray method for spheroids formation of hepatic cells

(2018) Binbin Zhang et al.   JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE

A multi-throughput multi-organ-on-a-chip system on a plate formatted pneumatic pressure-driven medium circulation platform

(2018) T. Satoh et al.   LAB ON A CHIP

Highly efficient and expedited hepatic differentiation from human pluripotent stem cells by pure small-molecule cocktails

(2018) Cong Du et al.   Stem Cell Research & Therapy

A Chimeric Humanized Mouse Model by Engrafting the Human Induced Pluripotent Stem Cell-Derived Hepatocyte-Like Cell for the Chronic Hepatitis B Virus Infection

(2018) Lunzhi Yuan et al.   Frontiers in Microbiology

Genetic and Chemical Screenings Identify HDAC3 as a Key Regulator in Hepatic Differentiation of Human Pluripotent Stem Cells

(2018) Shuang Li et al.   Stem Cell Reports

Characterization of hepatocyte-based in vitro systems for reliable toxicity testing

(2018) Mathieu Vinken et al.   ARCHIVES OF TOXICOLOGY

3D human liver tissue from pluripotent stem cells displays stable phenotype in vitro and supports compromised liver function in vivo

(2018) Hassan Rashidi et al.   ARCHIVES OF TOXICOLOGY

The metabolic fate of fenclozic acid in chimeric mice with a humanized liver

(2018) Anja Ekdahl et al.   ARCHIVES OF TOXICOLOGY

Human iPS derived progenitors bioengineered into liver organoids using an inverted colloidal crystal poly (ethylene glycol) scaffold

(2018) Soon Seng Ng et al.   BIOMATERIALS

Expansion and differentiation of human hepatocyte-derived liver progenitor-like cells and their use for the study of hepatotropic pathogens

(2018) Gong-Bo Fu et al.   CELL RESEARCH

Hybrid poly-l -lactic acid/poly(ε-caprolactone) nanofibrous scaffold can improve biochemical and molecular markers of human induced pluripotent stem cell-derived hepatocyte-like cells

(2018) Naser Mobarra et al.   JOURNAL OF CELLULAR PHYSIOLOGY

Recapitulation of methotrexate hepatotoxicity with induced pluripotent stem cell-derived hepatocytes from patients with rheumatoid arthritis

(2018) Juryun Kim et al.   Stem Cell Research & Therapy

Reconstructing the Human Renal Vascular-Tubular Unit In Vitro

(2018) Samuel G. Rayner et al.   Advanced Healthcare Materials

Endogenous and xenobiotic metabolic stability of primary human hepatocytes in long-term 3D spheroid cultures revealed by a combination of targeted and untargeted metabolomics

(2017) Sabine U. Vorrink et al.   FASEB JOURNAL

CSH guidelines for the diagnosis and treatment of drug-induced liver injury

(2017) Yue-cheng Yu et al.   Hepatology International

A Scalable Approach for the Generation of Human Pluripotent Stem Cell-Derived Hepatic Organoids with Sensitive Hepatotoxicity Features

(2017) Malte Sgodda et al.   STEM CELLS AND DEVELOPMENT

Prediction of drug-induced immune-mediated hepatotoxicity using hepatocyte-like cells derived from human embryonic stem cells

(2017) Dong Eon Kim et al.   TOXICOLOGY

A novel humanized mouse lacking murine P450 oxidoreductase for studying human drug metabolism

(2017) Mercedes Barzi et al.   Nature Communications

Massive and Reproducible Production of Liver Buds Entirely from Human Pluripotent Stem Cells

(2017) Takanori Takebe et al.   Cell Reports

An In Vitro Human Liver Model by iPSC-Derived Parenchymal and Non-parenchymal Cells

(2017) Yuta Koui et al.   Stem Cell Reports

Metabolic zonation of the liver: The oxygen gradient revisited

(2017) Thomas Kietzmann   Redox Biology

Multi-tissue interactions in an integrated three-tissue organ-on-a-chip platform

(2017) Aleksander Skardal et al.   Scientific Reports

Can Humanized Mice Predict Drug “Behavior” in Humans?

(2016) Dan Xu et al.   Annual Review of Pharmacology and Toxicology

Current limitations and future opportunities for prediction of DILI from in vitro

(2016) Christoph Funk et al.   ARCHIVES OF TOXICOLOGY

Self-assembled 3D spheroids and hollow-fibre bioreactors improve MSC-derived hepatocyte-like cell maturation in vitro

(2016) Madalena Cipriano et al.   ARCHIVES OF TOXICOLOGY

Fluid shear stress modulation of hepatocyte-like cell function

(2016) Hassan Rashidi et al.   ARCHIVES OF TOXICOLOGY

Modeling Development and Disease with Organoids

(2016) Hans Clevers   CELL

Chemically induced hepatotoxicity in human stem cell-induced hepatocytes compared with primary hepatocytes and HepG2

(2016) Seok-Jin Kang et al.   CELL BIOLOGY AND TOXICOLOGY

The HepaRG cell line, a superior in vitro model to L-02, HepG2 and hiHeps cell lines for assessing drug-induced liver injury

(2016) Yu Wu et al.   CELL BIOLOGY AND TOXICOLOGY

A comparison between genetically humanized and chimeric liver humanized mouse models for studies in drug metabolism and toxicity

(2016) Nico Scheer et al.   DRUG DISCOVERY TODAY

Organoids: A historical perspective of thinking in three dimensions

(2016) Marina Simian et al.   JOURNAL OF CELL BIOLOGY

The potential of induced pluripotent stem cell derived hepatocytes

(2016) Zara Hannoun et al.   JOURNAL OF HEPATOLOGY

Functionally Enhanced Human Stem Cell Derived Hepatocytes in Galactosylated Cellulosic Sponges for Hepatotoxicity Testing

(2016) Farah Tasnim et al.   MOLECULAR PHARMACEUTICS

Organoids as an in vitro model of human development and disease

(2016) Aliya Fatehullah et al.   NATURE CELL BIOLOGY

High-efficiency cellular reprogramming with microfluidics

(2016) Camilla Luni et al.   NATURE METHODS

Deterministically patterned biomimetic human iPSC-derived hepatic model via rapid 3D bioprinting

(2016) Xuanyi Ma et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

MicroRNA Regulates Hepatocytic Differentiation of Progenitor Cells by Targeting YAP1

(2016) Kwang Hwa Jung et al.   STEM CELLS

Advantageous use of HepaRG cells for the screening and mechanistic study of drug-induced steatosis

(2016) Laia Tolosa et al.   TOXICOLOGY AND APPLIED PHARMACOLOGY

Efficient generation of hepatic cells from mesenchymal stromal cells by an innovative bio-microfluidic cell culture device

(2016) Meng-Hua Yen et al.   Stem Cell Research & Therapy

Concise Review: Organoids Are a Powerful Tool for the Study of Liver Disease and Personalized Treatment Design in Humans and Animals

(2016) Sathidpak Nantasanti et al.   Stem Cells Translational Medicine

Synergistic effects of carboxymethyl-hexanoyl chitosan, cationic polyurethane-short branch PEI in miR122 gene delivery: Accelerated differentiation of iPSCs into mature hepatocyte-like cells and improved stem cell therapy in a hepatic failure model

(2015) Yueh Chien et al.   Acta Biomaterialia

Human mesenchymal stem cells - current trends and future prospective

(2015) Imran Ullah et al.   BIOSCIENCE REPORTS

Long-Term Culture of Genome-Stable Bipotent Stem Cells from Adult Human Liver

(2015) Meritxell Huch et al.   CELL

Signalling pathways involved in the process of mesenchymal stem cells differentiating into hepatocytes

(2015) Jun-Song Ye et al.   CELL PROLIFERATION

Efficient Engraftment of Human Induced Pluripotent Stem Cell-Derived Hepatocyte-Like Cells in uPA/SCID Mice by Overexpression of FNK, a Bcl-xLMutant Gene

(2015) Yasuhito Nagamoto et al.   CELL TRANSPLANTATION

Modeling mouse and human development using organoid cultures

(2015) M. Huch et al.   DEVELOPMENT

FGF2 mediates hepatic progenitor cell formation during human pluripotent stem cell differentiation by inducing the WNT antagonist NKD1

(2015) Kirk Twaroski et al.   GENES & DEVELOPMENT

Microbial-derived lithocholic acid and vitamin K2drive the metabolic maturation of pluripotent stem cells-derived and fetal hepatocytes

(2015) Yishai Avior et al.   HEPATOLOGY

Three-Dimensional Cell Culture: A Breakthrough in Vivo

(2015) Delphine Antoni et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Gene networks and transcription factor motifs defining the differentiation of stem cells into hepatocyte-like cells

(2015) Patricio Godoy et al.   JOURNAL OF HEPATOLOGY

Phenotypic and functional analyses show stem cell-derived hepatocyte-like cells better mimic fetal rather than adult hepatocytes

(2015) Melissa Baxter et al.   JOURNAL OF HEPATOLOGY

A four-organ-chip for interconnected long-term co-culture of human intestine, liver, skin and kidney equivalents

(2015) Ilka Maschmeyer et al.   LAB ON A CHIP

A lung-on-a-chip array with an integrated bio-inspired respiration mechanism

(2015) Andreas O. Stucki et al.   LAB ON A CHIP

Contributions of microbiome and mechanical deformation to intestinal bacterial overgrowth and inflammation in a human gut-on-a-chip

(2015) Hyun Jung Kim et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Morphological and Functional Characterization and Assessment of iPSC-Derived Hepatocytes forIn VitroToxicity Testing

(2015) Jingtao Lu et al.   TOXICOLOGICAL SCIENCES

Prediction of Drug-Induced Liver Injury in Micropatterned Co-cultures Containing iPSC-Derived Human Hepatocytes

(2015) Brenton R. Ware et al.   TOXICOLOGICAL SCIENCES

Rapid and high-efficiency generation of mature functional hepatocyte-like cells from adipose-derived stem cells by a three-step protocol

(2015) Fen Xu et al.   Stem Cell Research & Therapy

Small-Molecule-Driven Hepatocyte Differentiation of Human Pluripotent Stem Cells

(2015) Richard Siller et al.   Stem Cell Reports

ACG Clinical Guideline: The Diagnosis and Management of Idiosyncratic Drug-Induced Liver Injury

(2014) Naga P Chalasani et al.   AMERICAN JOURNAL OF GASTROENTEROLOGY

Direct Reprogramming of Human Fibroblasts to Functional and Expandable Hepatocytes

(2014) Pengyu Huang et al.   Cell Stem Cell

Competency of different cell models to predict human hepatotoxic drugs

(2014) M José Gómez-Lechón et al.   Expert Opinion on Drug Metabolism & Toxicology

Hepatic organoids for microfluidic drug screening

(2014) Sam H. Au et al.   LAB ON A CHIP

Prediction of interindividual differences in hepatic functions and drug sensitivity by using human iPS-derived hepatocytes

(2014) Kazuo Takayama et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Organogenesis in a dish: Modeling development and disease using organoid technologies

(2014) M. A. Lancaster et al.   SCIENCE

Fialuridine Induces Acute Liver Failure in Chimeric TK-NOG Mice: A Model for Detecting Hepatic Drug Toxicity Prior to Human Testing

(2014) Dan Xu et al.   PLOS MEDICINE

A mouse with humanized liver as an animal model for predicting drug effects and for studying hepatic viral infection: where to next?

(2013) Katsutoshi Yoshizato et al.   Expert Opinion on Drug Metabolism & Toxicology

Mice With Human Livers

(2013) Markus Grompe et al.   GASTROENTEROLOGY

Biomaterials for liver tissue engineering

(2013) Era Jain et al.   Hepatology International

Efficient generation of functional hepatocyte-like cells from menstrual blood-derived stem cells

(2013) Sayeh Khanjani et al.   Journal of Tissue Engineering and Regenerative Medicine

Vascularized and functional human liver from an iPSC-derived organ bud transplant

(2013) Takanori Takebe et al.   NATURE

Identification of small molecules for human hepatocyte expansion and iPS differentiation

(2013) Jing Shan et al.   Nature Chemical Biology

Can ‘humanized’ mice improve drug development in the 21st century?

(2013) Gary Peltz   TRENDS IN PHARMACOLOGICAL SCIENCES

Investigation of ifosfamide nephrotoxicity induced in a liver-kidney co-culture biochip

(2012) Leila Choucha-Snouber et al.   BIOTECHNOLOGY AND BIOENGINEERING

Stem cell-derived hepatocytes as a predictive model for drug-induced liver injury: are we there yet?

(2012) Richard Kia et al.   BRITISH JOURNAL OF CLINICAL PHARMACOLOGY

Characterization of primary human hepatocytes, HepG2 cells, and HepaRG cells at the mRNA level and CYP activity in response to inducers and their predictivity for the detection of human hepatotoxins

(2012) H. H. J. Gerets et al.   CELL BIOLOGY AND TOXICOLOGY

Comparison of in vitro hepatogenic differentiation potential between various placenta-derived stem cells and other adult stem cells as an alternative source of functional hepatocytes

(2012) Hyun-Jung Lee et al.   DIFFERENTIATION

Generation of metabolically functioning hepatocytes from human pluripotent stem cells by FOXA2 and HNF1α transduction

(2012) Kazuo Takayama et al.   JOURNAL OF HEPATOLOGY

Assessment of an automated in vitro basal cytotoxicity test system based on metabolically-competent cells

(2012) Robim M. Rodrigues et al.   TOXICOLOGY IN VITRO

Chimeric mice with a humanized liver as an animal model of troglitazone-induced liver injury

(2012) Masakazu Kakuni et al.   TOXICOLOGY LETTERS

Efficient Generation of Functional Hepatocytes From Human Embryonic Stem Cells and Induced Pluripotent Stem Cells by HNF4α Transduction

(2011) Kazuo Takayama et al.   MOLECULAR THERAPY

Induction of functional hepatocyte-like cells from mouse fibroblasts by defined factors

(2011) Pengyu Huang et al.   NATURE

Diagnosis, management and prevention of drug-induced liver injury

(2009) S Verma et al.   GUT

Functional expression, inhibition and induction of CYP enzymes in HepaRG cells

(2009) Miia Turpeinen et al.   TOXICOLOGY IN VITRO

Highly efficient differentiation of hESCs to functional hepatic endoderm requires ActivinA and Wnt3a signaling

(2008) D. C. Hay et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Native Umbilical Cord Matrix Stem Cells Express Hepatic Markers and Differentiate Into Hepatocyte-like Cells

(2007) David Campard et al.   GASTROENTEROLOGY