Review
Chemistry, Physical
Shuyu Zhang, Guoshi Xu, Juan Wu, Xiao Liu, Yong Fan, Jun Chen, Gordon Wallace, Qi Gu
Summary: In recent years, significant advancements have been made in microphysiological constructs and systems (MPCs and MPSs), providing alternatives to animal models for drug discovery and personalized medicine. However, current approaches mainly focus on the in vitro recapitulation of the human anatomical structure and physiological-biochemical indices at a single or a few simple levels.
Review
Biotechnology & Applied Microbiology
Luana A. Osorio, Elisabete Silva, Ruth E. Mackay
Summary: Organ-on-a-Chip (OOAC) systems are engineered microfluidic systems that mimic the physiological environment of specific organs and have the potential to replace animal models. The success of cell cultures within these systems depends on the choice of scaffold, highlighting the critical role of scaffolds in OOAC research.
BIOENGINEERING-BASEL
(2021)
Review
Pharmacology & Pharmacy
Islam M. Adel, Mohamed F. ElMeligy, Nermeen A. Elkasabgy
Summary: Tissue regeneration is an auto-healing mechanism that aims to restore damaged tissue structure and function. However, the process is slow, which led to the development of tissue engineering. Scaffold, a matrix used to support tissue growth, plays a crucial role in tissue engineering. The future prospects of scaffold integration include nanotechnology and 4D printing.
Review
Cell Biology
Leandra S. Baptista, Constance Porrini, Gabriela S. Kronemberger, Daniel J. Kelly, Cecile M. Perrault
Summary: Medicine today faces the challenge of increasing untreatable diseases and a decrease in successful drug development. In vitro preclinical tests, particularly using 3D cell culture and human stem cell biology, can help predict the potential of new drugs and avoid expensive clinical trial phases. Integrating organoid culture with microsystems, such as microphysiological systems or organ-on-a-chip, can improve drug development by emulating physiological conditions and reducing costs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Martin Trapecar
Summary: Metabolic and inflammatory disorders like autoimmune and neurodegenerative diseases are on the rise, with the use of microphysiological systems offering new possibilities to study these complex systemic pathologies. Controlled interactions between multiple MPSs, scalability of biological complexity in MPSs, and continuous multiomic monitoring may unlock novel relationships between interorgan crosstalk, metabolism, and immunity.
Article
Multidisciplinary Sciences
Sadegh Seidi, Aziz Eftekhari, Ameer Khusro, Reza Shiri Heris, Muhammad Umar Khayam Sahibzada, Mario Gajdacs
Summary: Organ-on-a-chip biosystems are microfluidic devices that mimic the functions of different organs and create physiological models. They have diverse applications in the field of biomedicine and are crucial for disease modeling and drug development.
JOURNAL OF KING SAUD UNIVERSITY SCIENCE
(2022)
Review
Chemistry, Multidisciplinary
Hanie Kavand, Rohollah Nasiri, Anna Herland
Summary: Advanced in vitro cell culture systems and microphysiological systems (MPSs) are breakthrough technologies in biomedicine that replicate features of human tissues. The use of advanced functional materials and devices could enable better reproduction of in vivo-like functionality and real-time monitoring of tissue function, leading to more accurate mimicry of human physiology.
ADVANCED MATERIALS
(2022)
Review
Chemistry, Analytical
Leslie Donoghue, Khanh T. Nguyen, Caleb Graham, Palaniappan Sethu
Summary: This review discusses tissue chips and microphysiological systems, highlighting their basic definitions, major organs/tissues, critical parameters, and microfluidic approaches. It addresses current shortcomings and future directions of these technologies.
Article
Engineering, Biomedical
Vardhman Kumar, David Kingsley, Sajeeshkumar Madhurakkat Perikamana, Pankaj Mogha, C. Rory Goodwin, Shyni Varghese
Summary: This study presents a microfluidic-assisted tissue model of nociception with microvasculature. The neurons and endothelial cells displayed distinct morphologies in the presence of each other. The neurons exhibited an elevated response to capsaicin in the presence of vasculature, and increased TRPV1 receptor expression was observed in the neurons. The platform could also be used to study pain associated with tissue acidosis and pave the way for the development of innervated microphysiological models.
Review
Biochemical Research Methods
Sung-Min Kang
Summary: Microphysiological systems are in vitro models of tissues and organs that help in understanding human physiology and pathophysiology by replicating specific organ structures and functions. Recent advances in these systems using microfluidic technologies allow for deeper insights into complex in vivo physiological phenomena and mechanisms.
Article
Engineering, Biomedical
Samuel G. Rayner, Caitlin C. Howard, Christian J. Mandrycky, Stefan Stamenkovic, Jonathan Himmelfarb, Andy Y. Shih, Ying Zheng
Summary: The use of multiphoton ablation technology combined with guided endothelial cell growth from pre-formed microvessels has successfully created perfusable and cellularized organ-specific microvascular structures within collagen hydrogels, opening new possibilities for engineering tissues and organ-on-a-chip devices in vitro.
ADVANCED HEALTHCARE MATERIALS
(2021)
Review
Cell Biology
Mridu Malik, Yang Yang, Parinaz Fathi, Gretchen J. Mahler, Mandy B. Esch
Summary: Traditional animal models for drug identification are facing criticism for their reliability in predicting drug toxicity and efficacy in humans. Multi-organ microphysiological systems (MPS) are considered as alternative models which can simulate diseases and evaluate the efficacy and safety of drug candidates, but accuracy in organ scaling and flow rates is crucial.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Orthopedics
L. Banh, K. K. Cheung, M. W. Y. Chan, E. W. K. Young, S. Viswanathan
Summary: Joint-on-a-chip models are powerful tools for osteoarthritis research, recapitulating the multifaceted joint tissue microenvironment. This article reviews recent advancements in human Joint-on-a-chip technology and identifies gaps for future development, including mechanical stimulation systems, multi-joint tissue cultures, and incorporation of immune cells to capture aspects of osteoarthritis inflammation.
OSTEOARTHRITIS AND CARTILAGE
(2022)
Article
Gastroenterology & Hepatology
John D. O'Neill, Meghan R. Pinezich, Brandon A. Guenthart, Gordana Vunjak-Novakovic
Summary: The burden of gastrointestinal diseases is increasing globally, leading to a focus on bioengineering strategies for gut tissue regeneration. While progress has been made in intestinal organoid cultures and engineered tissues, the development of predictive in vitro models and effective regenerative therapies remains challenging in gut bioengineering. This review surveys emerging bioengineering tools and recent methodological advances, identifying current challenges and future opportunities in the field.
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
(2021)
Article
Engineering, Biomedical
Drew E. Glaser, Matthew B. Curtis, Peter A. Sariano, Zachary A. Rollins, Bhupinder S. Shergill, Aravind Anand, Alyssa M. Deely, Venktesh S. Shirure, Leif Anderson, Jeremy M. Lowen, Natalie R. Ng, Katherine Weilbaecher, Daniel C. Link, Steven C. George
Summary: A 3D in vitro model of human bone marrow containing perivascular and endosteal niches has been developed using microfluidic and stem cell technologies. This model can replicate in vivo bone marrow function and study dynamic events at the niche level, providing opportunities to advance our understanding of human bone marrow function and drug response.
Article
Engineering, Biomedical
Samuel G. Rayner, Caitlin C. Howard, Christian J. Mandrycky, Stefan Stamenkovic, Jonathan Himmelfarb, Andy Y. Shih, Ying Zheng
Summary: The use of multiphoton ablation technology combined with guided endothelial cell growth from pre-formed microvessels has successfully created perfusable and cellularized organ-specific microvascular structures within collagen hydrogels, opening new possibilities for engineering tissues and organ-on-a-chip devices in vitro.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Cell & Tissue Engineering
Yoon Jung Choi, Adam M. Heck, Brian J. Hayes, Daniel Lih, Samuel G. Rayner, Brandon Hadland, Ying Zheng
Summary: The human fetal liver endothelium plays a unique role in supporting the maturation and expansion of multilineage hematopoietic stem and progenitor cells, with EC-derived WNT5A identified as a key factor in this process. The study highlights the significance of organ-specific endothelial niche in hematopoietic development and provides insights into potential signals for HSPC expansion in clinical applications.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Cardiac & Cardiovascular Systems
Cecilia Berardi, David A. Bluemke, Brian A. Houston, Todd M. Kolb, Joao A. Lima, Theo Pezel, Ryan J. Tedford, Samuel G. Rayner, Richard K. Cheng, Peter J. Leary
Summary: The study revealed that sFlt-1 levels are associated with incident heart failure and cardiovascular mortality. There is also an association between sFlt-1 levels and cardiac mass.
JOURNAL OF HEART AND LUNG TRANSPLANTATION
(2022)
Review
Cardiac & Cardiovascular Systems
Christian J. Mandrycky, Caitlin C. Howard, Samuel G. Rayner, Yu Jung Shin, Ying Zheng
Summary: Organ-on-a-chip platforms involve miniaturization of cell culture systems, enabling novel experimental approaches such as modeling biophysical forces on cells and screening new drugs. Vascular structure is a key feature in these systems, with recent advances focusing on developing vascular models for studying vascular biology and tissue regeneration. Future approaches aim to select fit-for-purpose OOC models to either serve as simple testbeds for therapeutic development or accurately replicate human physiology for specific disease modeling and tissue regeneration.
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2021)
Article
Hematology
Samuel G. Rayner, Zackary Scholl, Christian J. Mandrycky, Junmei Chen, Karina N. LaValley, Peter J. Leary, William A. Altemeier, W. Conrad Liles, Dominic W. Chung, Jose A. Lopez, Hongxia Fu, Ying Zheng
Summary: This study investigates the interaction between VWF and fibrin and suggests that rapid release of endothelial VWF during clotting may support fibrin polymerization and accelerate thrombosis.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2022)
Review
Nanoscience & Nanotechnology
Colleen O'Connor, Eileen Brady, Ying Zheng, Erika Moore, Kelly R. Stevens
Summary: This Review discusses how materials advances have facilitated the development of vascular engineering approaches, driven by both technology and nature, to create vascular networks for various applications. It emphasizes the importance of understanding vascular structure and highlights new methods such as bioprinting, organoids and microfluidic systems for fabricating 3D vascular topologies at a cellular scale with lumen perfusion. The Review also highlights the remaining knowledge gaps and opportunities in this field.
NATURE REVIEWS MATERIALS
(2022)
Letter
Cardiac & Cardiovascular Systems
Hongyang Pi, Selma D. Carlson, Lia M. Barros, Laurie Hogl, James N. Kirkpatrick, Stephanie Nolley, David D. Ralph, Samuel G. Rayner, Peter J. Leary
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2022)
Article
Respiratory System
Hongyang Pi, Samuel G. Rayner, David D. Ralph, Stephanie Nolley, Lia M. Barros, Zachary L. Steinberg, Peter J. Leary
Summary: This observational study examined the association between thyroid-stimulating hormone (TSH) and mortality in individuals with pulmonary arterial hypertension (PAH). The results showed a strong association between TSH and mortality, regardless of adjustment. However, there was no consistent association between TSH and other markers of disease severity in the PAH cohort.
BMJ OPEN RESPIRATORY RESEARCH
(2022)
Article
Cardiac & Cardiovascular Systems
Hongyang Pi, Lu Xia, David D. Ralph, Samuel G. Rayner, Ali Shojaie, Peter J. Leary, Sina A. Gharib
Summary: In this study, metabolic profiling was used to identify the association between plasma metabolomic profiles and right ventricular dilation, mortality, and measures of disease severity in patients with pulmonary arterial hypertension (PAH). The polyamine, histidine, and sphingomyelin metabolic pathways were found to be associated with RV dilation, mortality, and disease severity.
CIRCULATION RESEARCH
(2023)
Article
Cardiac & Cardiovascular Systems
Kellen Hirsch, Stephanie Nolley, David D. Ralph, Ying Zheng, William A. Altemeier, Christopher J. Rhodes, Nicholas W. Morrell, Martin R. Wilkins, Peter J. Leary, Samuel G. Rayner
Summary: This study compared the differences in inflammatory and angiogenic biomarker profiles among patients with different etiologies of pulmonary arterial hypertension (PAH) and their association with clinical outcomes. The results showed that patients with connective tissue disease-associated PAH (CTD-PAH) had significantly elevated levels of inflammatory and angiogenic biomarkers. Six biomarkers were associated with mortality in the entire PAH cohort, with IL-6 and NT-proBNP remaining significant after controlling for multiple comparisons.
JOURNAL OF HEART AND LUNG TRANSPLANTATION
(2023)
Editorial Material
Critical Care Medicine
Despina Michailidou, Delphine L. Chen, Bridget F. Collins, Marjorie P. George, David Montani, Samuel G. Rayner, Chanprasert Sirisak, Peter J. Leary
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2023)
Article
Cardiac & Cardiovascular Systems
Lauren N. Carlozzi, Josh Lee, Lia M. Barros, Yonatan Buber, Delphine L. Chen, Michael Mulligan, Karen Ordovas, David D. Ralph, Samuel G. Rayner, Peter J. Leary, Zachary L. Steinberg
Summary: This report presents an early experience with BPA at a single US center. The study found that BPA can improve non-invasive and invasive metrics in patients with chronic thromboembolic pulmonary hypertension, without adding significant morbidity to a high-risk patient population.
RESPIRATORY MEDICINE
(2023)
Review
Physiology
Samuel G. Rayner, Chi F. Hung, W. Conrad Liles, William A. Altemeier
Summary: Pericytes are microvascular mural cells that play a significant role in vascular development and homeostasis. They are also key mediators of the host response to injury, displaying cellular plasticity and involvement in inflammatory processes.
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2023)
Article
Engineering, Biomedical
Nicole Zeinstra, Ariana L. Frey, Zhiying Xie, Leslie P. Blakely, Ruikang K. Wang, Charles E. Murry, Ying Zheng
Summary: A modular fabrication approach using stacked patterned collagen membranes is reported to generate thick, large, three-dimensional, perfusable networks of endothelialized vasculature. The perfusable vascular networks show remodeling and evenly distributed perfusion in vitro while maintaining their patterned, open-lumen architecture. Implanted onto infarcted rat hearts, the perfusable vascular networks achieve greater host vascular integration and higher perfusion rate compared to self-assembled controls. This success represents an important step forward for vascular bioengineering and opens possibilities for more complex, large scale, highly metabolic engineered tissues.
Article
Multidisciplinary Sciences
Sijie Sun, Lei Jin, Ying Zheng, Jia Zhu
Summary: A vascularized 3D "skin-on-chip" model has been developed to mimic human skin architecture and investigate Herpes simplex virus (HSV) infection, showing that perfusion with an antiviral drug can inhibit the infection.
NATURE COMMUNICATIONS
(2022)