Article
Materials Science, Biomaterials
Ashwini Vasudevan, Nilotpal Majumder, Indu Sharma, Impreet Kaur, Subramanian Sundarrajan, Jayarama Reddy Venugopal, Pooja Vijayaraghavan, Neetu Singh, Seeram Ramakrishna, Sourabh Ghosh, Dinesh Tripathi, Savneet Kaur
Summary: Liver-specific nanofiber scaffolds fabricated using electrospinning technique can prolong the viability and functionality of primary hepatocytes in culture, improve cell survival rate and functionality, and serve as an effective platform for predicting in vivo drug-induced hepatotoxicity.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Review
Engineering, Biomedical
Tejas M. Koushik, Catherine M. Miller, Elsa Antunes
Summary: Bone tissue engineering has become a well-known field of study in the past decade, with advancements in materials, processing techniques, and understanding of bone healing pathways. Synthetic scaffolds face the challenge of providing load-bearing capability and interaction with the local extracellular matrix for promoting bone healing. This article discusses the usage and processing of multi-materials and hierarchical structures to mimic the structure of natural bone tissues for bioactive and load-bearing synthetic scaffolds.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Engineering, Biomedical
Sarah Girardeau-Hubert, Barbara Lynch, Francesca Zuttion, Rabab Label, Chrystelle Rayee, Sebastien Brizion, Sylvie Ricois, Anthony Martinez, Eunhye Park, Changhwan Kim, Paulo Andre Marinho, Jin-Hyung Shim, Songwan Jin, Maite Rielland, Jeremie Soeur
Summary: This study focuses on the mechanical properties, extra-cellular matrix organization, and cell interactions in human skin samples reconstructed with pure collagen or dermal decellularized extra-cellular matrices (S-dECM). The results show that the S-dECM samples have higher stiffness compared to collagen samples and exhibit similarities to ex vivo human skin. The study demonstrates the importance of the microenvironment in influencing cell behaviors and mechanical properties.
ACTA BIOMATERIALIA
(2022)
Review
Polymer Science
Naiyu Cui, Chun-Yu Dai, Xuran Mao, Xun Lv, Yue Gu, Eui-Seok Lee, Heng-Bo Jiang, Yunhan Sun
Summary: Poloxamer, as a triblock copolymer with amphiphilicity and reversible thermal responsiveness, has wide application prospects in biomedicine. It plays a crucial role as injectable scaffolds in tissue engineering and 3D printing, creating an appropriate microenvironment for cell growth and vascular network formation.
Article
Chemistry, Physical
Weizhen Sun, David Alexander Gregory, Xiubo Zhao
Summary: Peptide amphiphiles (PAs) are peptide-based molecules that can self-assemble into well-ordered supramolecular nanostructures. They have diverse sequences and exhibit biocompatibility, biodegradability, and resemblance to native ECM, making them ideal scaffold materials for tissue engineering applications.
ADVANCES IN COLLOID AND INTERFACE SCIENCE
(2023)
Review
Polymer Science
Jingzhi Fan, Keyvan Abedi-Dorcheh, Asma Sadat Vaziri, Fereshteh Kazemi-Aghdam, Saeed Rafieyan, Masoume Sohrabinejad, Mina Ghorbani, Fatemeh Rastegar Adib, Zahra Ghasemi, Kristaps Klavins, Vahid Jahed
Summary: The musculoskeletal system is crucial for movement, organ protection, support, hematopoiesis, and postural stability. Damage to or loss of musculoskeletal tissues greatly affects quality of life. Natural polymers have shown great potential in tissue engineering for musculoskeletal repair.
Article
Engineering, Environmental
Meng Luo, Dagogo Dorothy Winston, Wen Niu, Yidan Wang, Hongyang Zhao, Xiaoyan Qu, Bo Lei
Summary: The study presented a multifunctional bioactive therapeutics-repair-enabled citrate-iron hydrogel scaffold that can inhibit tumor recurrence and promote wound repair simultaneously, showing great potential in clinical applications.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Nanoscience & Nanotechnology
Kateryna Diedkova, Alexander D. Pogrebnjak, Sergiy Kyrylenko, Kateryna Smyrnova, Vladimir V. Buranich, Pawel Horodek, Pawel Zukowski, Tomasz N. Koltunowicz, Piotr Galaszkiewicz, Kristina Makashina, Vitaly Bondariev, Martin Sahul, Maria Caplovicova, Yevheniia Husak, Wojciech Simka, Viktoriia Korniienko, Agnieszka Stolarczyk, Agata Blacha-Grzechnik, Vitalii Balitskyi, Veronika Zahorodna, Ivan Baginskiy, Una Riekstina, Oleksiy Gogotsi, Yury Gogotsi, Maksym Pogorielov
Summary: New conductive materials are needed for tissue engineering to develop regenerative strategies for nervous, muscular, and heart tissues. Polycaprolactone (PCL) is used to create biocompatible and biodegradable nanofiber scaffolds, while MXenes can make these scaffolds conductive and hydrophilic. This study investigates the physical properties, defect structure, and porosity of PCL-MXene composite scaffolds, and demonstrates their advantages over existing conductive scaffolds for tissue engineering in terms of structure, chemistry, electricity, and biology.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Materials Science, Multidisciplinary
Ryan J. J. Hickey, Maxime Leblanc Latour, James L. L. Harden, Andrew E. E. Pelling
Summary: This study demonstrates the manufacturing of modular blocks of naturally porous decellularized plant-derived scaffolds that can be seeded with different cell types to create complex tissue interfaces. The interaction between different cell types at the interface is investigated, and the biocompatibility of the engineered tissue interface (ETI) is demonstrated in vivo. This research opens up new avenues for tissue design and understanding biological processes.
ADVANCED ENGINEERING MATERIALS
(2023)
Review
Pharmacology & Pharmacy
Maria Lazaridou, Dimitrios N. Bikiaris, Dimitrios A. Lamprou
Summary: Bioprinting is a promising technology for developing functional tissue constructs and controlled drug delivery systems. Chitosan, a natural polysaccharide, is an attractive bioink for tissue regeneration and restoration.
Article
Biochemistry & Molecular Biology
Raul Llamas-Unzueta, Marta Suarez, Adolfo Fernandez, Raquel Diaz, Miguel A. Montes-Moran, J. Angel Menendez
Summary: Porous carbon structures derived from whey powders show promising potential as scaffolds in bone tissue engineering, with high porosity, hierarchical pore size distribution, exceptional compressive strength and elastic modulus, as well as non-cytotoxic and bioactive behavior due to their carbon-based composition.
Article
Materials Science, Biomaterials
Alex A. Volinsky, Oleg V. Kokorev, Ekaterina S. Marchenko, Igor A. Khlusov, Yuri F. Yasenchuk, Alexander N. Monogenov
Summary: Currently, it is relevant to use alternative systems for hepatic metabolism and partial replacement of liver organ failure, especially due to the increase in liver disorders and organ insufficiency for transplantation. Low-cost intracorporeal systems using tissue engineering for hepatic metabolism maintenance before transplantation or complete liver function replacement deserve attention. This study describes the in vivo application of fibrous nickel-titanium scaffolds (FNTS) with cultured hepatocytes, which showed superior liver function and survival in a cirrhosis rats' model compared to hepatocyte injections.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Michal Halperin-Sternfeld, Gal Netanel Liberman, Raha Kannan, Francesca Netti, Peter X. Ma, Shoshana Malis Arad, Lihi Adler-Abramovich
Summary: Sulfated polysaccharides from red marine microalgae, when combined with FmocFF peptide hydrogelator, form transparent and stable nanofibrous hydrogels. The mechanical properties and sustained release capabilities of these composite hydrogels can be tuned by changing the hydrogel concentration. This study demonstrates the potential of these hydrogels for tissue engineering and cell differentiation.
Review
Chemistry, Multidisciplinary
Xiaoying Luo, Xin He, Hui Zhao, Jun Ma, Jie Tao, Songjiao Zhao, Yan Yan, Yao Li, Shenmin Zhu
Summary: Currently, donor tissue is required for treating corneal diseases caused by corneal endothelium injury. However, there is a severe shortage of available corneas. Therefore, researchers are exploring alternative approaches, such as tissue-engineered scaffolds, which can support the transplantation of corneal endothelial cells. This review provides a comprehensive overview of recent advancements in using polymer biomaterials as scaffolds for corneal endothelium tissue engineering. It analyzes the key properties required for an effective corneal endothelial implant using polymer biomaterials, discusses various emerging biomaterials as scaffolds, and highlights the challenges and prospects of these materials in corneal endothelium tissue engineering.
Article
Agricultural Engineering
Chotiwit Sriwong, Suwimon Boonrungsiman, Prakit Sukyai
Summary: Biocomposite cellulose-based scaffolds combined with hydroxyapatite were successfully developed from sugarcane bagasse for tissue engineering. The scaffolds showed appropriate porosity and low degradation rate. Cell viability, proliferation, and differentiation experiments demonstrated that the cellulose combined HA scaffolds promoted the growth and differentiation of bone cells. The results indicated that sugarcane bagasse-derived cellulose is an ideal material for bone scaffolding applications.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Review
Biochemical Research Methods
Joyita Sarkar, Ashok Kumar
Summary: High-throughput systems are widely used in various fields such as bioprocess industries, pollutant detection, and material microarrays. The application of biomaterials in these systems has facilitated cell-based assays and the discovery of novel biomaterials. However, challenges in clinical applicability still exist, requiring focus on analysis techniques and storage/shelf-life of cell-laden microarrays.
BIOTECHNOLOGY JOURNAL
(2021)
Article
Biotechnology & Applied Microbiology
Priyanka Jayal, Padmanava Behera, Ranajoy Mullick, Subbaraya G. Ramachandra, Saumitra Das, Ashok Kumar, Anjali Karande
Summary: Three-dimensional cell culture models act as a bridge between traditional two-dimensional cell culture and animal models, providing the best model system for understanding the pathophysiology of human pathogens.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Chemistry, Applied
Kajal Bamnavat, Vinita Bhardwaj, Thangaraj Anand, S. K. Ashok Kumar, Suban K. Sahoo
Summary: pH determination is crucial in various biological and chemical processes. The manuscript explores the use of a pyridoxal derived Schiff base L for fluorescent pH sensing. Through the formation of self-aggregates and fluorescence analysis, the compound showed potential for pH detection in different ranges.
Article
Biochemistry & Molecular Biology
Sneha Gupta, Arun Kumar Teotia, Irfan Qayoom, Parvaiz Ahmad Shiekh, Syed Muntazir Andrabi, Ashok Kumar
Summary: The study developed a biomimetic periosteum membrane and a bioactive inorganic-organic composite cryogel for promoting periosteal regeneration and bone formation in in vitro oxidative stress model and rat tibial bone fracture model.
Article
Biotechnology & Applied Microbiology
Aman Nikhil, Ashok Kumar
Summary: This study investigated the potential of tissue-engineered cryogel scaffold for treating articular cartilage injuries, particularly osteochondral tissue. Different cryogels were fabricated for articular cartilage and subchondral bone, and a novel bilayer cryogel mimicking osteochondral unit was designed. The study evaluated biocompatibility, effectiveness of exosomes and cryogel extract in chondrocyte proliferation, migration, and potential for osteochondral repair.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Article
Engineering, Environmental
Prerna Singh, Syed Muntazir Andrabi, Ubaid Tariq, Sneha Gupta, Shazia Shaikh, Ashok Kumar
Summary: Chronic wounds are complex conditions characterized by delayed healing, persistent inflammation, oxidative stress, infections, and hypoxic conditions at the wound site. A versatile bilayer-type cryogel scaffold, incorporating bioactive factors and an iodine embedded antibacterial layer, was fabricated to efficiently and timely heal chronic wounds. The scaffold showed high-water uptake capacity, swelling ratio, and degradation rate, and demonstrated controlled release of iodine, oxygen, and nitric acid. In vitro studies demonstrated biocompatibility, antioxidant and cell migration potential, as well as efficacious antibacterial action. The in vivo studies further confirmed the scaffold's ability to expedite wound closure, promote tissue regeneration, and maintain native skin architecture. This combinatorial approach represents a promising strategy for the advanced treatment of complex wound conditions.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Huizhi Guo, Weidong Weng, Shuncong Zhang, Helen Rinderknecht, Bianca Braun, Regina Breinbauer, Purva Gupta, Ashok Kumar, Sabrina Ehnert, Tina Histing, Andreas K. Nussler, Romina H. Aspera-Werz
Summary: Maqui berry extract and ginseng extract can reduce bone cell damage caused by cigarette smoke and restore their function. These two natural antioxidants protect against CSE through the inhibition of the NF-kappa B signaling pathway and activation of the Nrf2 signaling pathway.
Article
Nanoscience & Nanotechnology
Sneha Gupta, Irfan Qayoom, Purva Gupta, Archita Gupta, Prerna Singh, Sneha Singh, Ashok Kumar
Summary: This study developed a one-step biomaterial-based cell-free approach using an exosome carrier and a CQ extract-laden herbal membrane for simultaneous management of bone formation and periosteal development in osteosarcoma. In vitro and in vivo experiments demonstrated the efficacy of the approach in killing tumor cells, promoting bone formation, and developing periosteum, preventing further fractures.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biochemistry & Molecular Biology
Shazia Shaikh, Hossein Baniasadi, Shreya Mehrotra, Rupita Ghosh, Prerna Singh, Jukka V. Seppala, Ashok Kumar
Summary: This study focuses on addressing the limitations of traditional metal-alloy bone fixation devices by fabricating biodegradable composites using poly(lactic acid) (PLA) and strontium-substituted nanohydroxyapatite (SrHAP). The composites demonstrate improved mechanical properties, formability, and radiopacity, making them suitable as implant materials for orthopedic applications.
Article
Chemistry, Physical
Ekta Srivastava, Irfan Qayoom, Anamika Singh, Ashok Kumar
Summary: The study introduces a new type of Electroband by reducing graphene oxide (rGO) on its surface. The Electroband, combined with in-situ electrical stimulation, promotes nerve regeneration through neuroprotection, neuroregeneration, and neuroplasticity in a rat median nerve injury model, achieving significant results.
Article
Engineering, Environmental
Rupita Ghosh, Shazia Shaikh, Sneha Gupta, Shreya Mehrotra, Ashok Kumar
Summary: In this study, Mg-doped calcium silicate ceramic diopside was used as a ceramic matrix material, and rare earth lanthanum phosphate was introduced as a reinforcement to improve machinability. The composites were sintered at different temperatures and conditions, and the double sintered composites showed the highest densification and mechanical properties. The optimized ceramic composites exhibited machinability, bioactivity, biocompatibility, and osteoconductivity.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Paul Nana Kwame Sagoe, Eduardo Jose Machado Velazquez, Yohely Maria Espiritusanto, Amelia Gilbert, Thalma Orado, Qiu Wang, Era Jain
Summary: In this study, we developed a novel method for synthesizing tunable-sized microparticles with improved monodispersity and batch-to-batch reproducibility. We evaluated the effect of various process parameters on particle size, distribution, and morphology. By adjusting key parameters, we achieved microparticles of comparable sizes across different formulations. The study also demonstrated the influence of polymer concentration, stirring rate, and surfactant concentration on microparticle characteristics.
Editorial Material
Chemistry, Medicinal
Silviya Petrova Zustiak, Era Jain
Article
Engineering, Biomedical
Ritu Gupta, Sneha Gupta, Purva Gupta, Andreas K. Nuessler, Ashok Kumar
Summary: Extracellular vesicles (EVs) are nano-sized vehicles secreted by all live cells to communicate with adjacent cells. Plant-derived exosome-like nanovesicles (PDEVs) may be a better substitute for mammalian EVs (MEVs) due to their widespread sources, cost-effectiveness, and accessibility. This study isolated exosomes from callus tissue derived from Cissus quadrangularis (CQ) and explored their therapeutic potential in wound healing and oxidative stress conditions. The CQ exosomes showed promise in improving these conditions in human-derived mesenchymal stem cells and pre-osteoblast cell line. These findings suggest that CQ exosomes could be used as nanocarriers for drug molecules in bone applications.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Review
Engineering, Biomedical
Ankita Das, Shreya Mehrotra, Ashok Kumar
Summary: Coronary artery disease is a common cardiovascular disease, and the current treatment methods have limitations. This review summarizes the shortcomings of commercial metal stents and introduces the use of biodegradable polymers to create personalized stents. The functionalization of stent surfaces to enhance their performance is also discussed.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)