Enzyme Engineering Strategies for the Bioenhancement of l-Asparaginase Used as a Biopharmaceutical

Golgi fucosyltransferase 1 reveals its important role in α-1,4-fucose modification of N-glycan in CRISPR/Cas9 diatom Phaeodactylum tricornutum

(2023) Xihui Xie et al.   Microbial Cell Factories

The anticancer effect of PASylated calreticulin-targeting L-ASNase in solid tumor bearing mice with immunogenic cell death-inducing chemotherapy

(2023) Ying Zhang et al.   BIOCHEMICAL PHARMACOLOGY

Challenges and progress towards industrial recombinant protein production in yeasts: A review

(2023) Pieter De Brabander et al.   BIOTECHNOLOGY ADVANCES

In Silico Design of a Chimeric Humanized L-asparaginase

(2023) Alejandro Pedroso et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Antarctic yeasts as a source of L-asparaginase: Characterization of a glutaminase-activity free L-asparaginase from psychrotolerant yeast Leucosporidium scottii L115

(2023) Ignacio Sánchez-Moguel et al.   PROCESS BIOCHEMISTRY

Treatment and outcomes of symptomatic hyperammonemia following asparaginase therapy in children with acute lymphoblastic leukemia

(2023) Angela Lee et al.   MOLECULAR GENETICS AND METABOLISM

Downstream process and evaluation of the concomitant impact of a recombinant glycosylated L-asparaginase on leukemic cancer cells and the bone marrow tumor microenvironment

(2023) Eduardo Krebs Kleingesinds et al.   PROCESS BIOCHEMISTRY

Plant asparaginase versus microbial asparaginase as anticancer agent

(2022) Nawal E. Al-Hazmi et al.   ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH

Novel Insights on the Use of L-Asparaginase as an Efficient and Safe Anti-Cancer Therapy

(2022) Maaike Van Trimpont et al.   Cancers

A critical review on exploitation of agro-industrial biomass as substrates for the therapeutic microbial enzymes production and implemented protein purification techniques

(2022) Diksha Raina et al.   CHEMOSPHERE

Microbial L-asparaginase as a promising enzyme for treatment of various cancers

(2022) Farshad Darvishi et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Nanoparticle-based medicines in clinical cancer therapy

(2022) Shuhang Wang et al.   Nano Today

Molecular cloning, characterization, and in-silico analysis of l-asparaginase from Himalayan Pseudomonas sp. PCH44

(2022) Subhash Kumar et al.   3 Biotech

Exploring current scenario and developments in the field of microbial L-asparaginase production and applications: A review

(2022) Payal G. Patel et al.   PROCESS BIOCHEMISTRY

Highly efficient and simple SSPER and rrPCR approaches for the accurate site-directed mutagenesis of large and small plasmids

(2022) Huiyong Jia et al.   New Biotechnology

Therapeutic enzymes: Discoveries, Production and Applications

(2021) Siddhi Tandon et al.   JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY

Bioinformatic analysis and genetic engineering approaches for recombinant biopharmaceutical glycoproteins production in microalgae

(2021) Xiaojuan Liu et al.   Algal Research-Biomass Biofuels and Bioproducts

L-asparaginase production review: bioprocess design and biochemical characteristics

(2021) Daniel Castro et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Increased glycosylated l-asparaginase production through selection of Pichia pastoris platform and oxygen-methanol control in fed-batches

(2021) Letícia de Almeida Parizotto et al.   BIOCHEMICAL ENGINEERING JOURNAL

Circumventing the side effects of L-asparaginase

(2021) Marcela Helena Gambim Fonseca et al.   BIOMEDICINE & PHARMACOTHERAPY

Microbial production, molecular modification, and practical application of l-Asparaginase: A review

(2021) Yiming Wang et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Exosomes as Naturally Occurring Vehicles for Delivery of Biopharmaceuticals: Insights from Drug Delivery to Clinical Perspectives

(2021) Arun Butreddy et al.   Nanomaterials

Structural and biophysical aspects of L-asparaginases: a growing family with amazing diversity

(2021) Joanna I. Loch et al.   IUCrJ

Low-cost agro-industrial sources as a substrate for the production of l-asparaginase using filamentous fungi

(2021) Maysa Lima Parente Fernandes et al.   Biocatalysis and Agricultural Biotechnology

Combining Ancestral Reconstruction with Folding-Landscape Simulations to Engineer Heterologous Protein Expression

(2021) Gloria Gamiz-Arco et al.   JOURNAL OF MOLECULAR BIOLOGY

A combination of bioinformatics analysis and rational design strategies to enhance keratinase thermostability for efficient biodegradation of feathers

(2021) Chang Su et al.   SCIENCE OF THE TOTAL ENVIRONMENT

Microbial enzymes for deprivation of amino acid metabolism in malignant cells: biological strategy for cancer treatment

(2020) Rakhi Dhankhar et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Critical overview of the main features and techniques used for the evaluation of the clinical applicability of L-asparaginase as a biopharmaceutical to treat blood cancer

(2020) T.A. Costa-Silva et al.   BLOOD REVIEWS

Glycosylation of L-asparaginase from E. coli through yeast expression and site-directed mutagenesis

(2020) Guilherme Meira Lima et al.   BIOCHEMICAL ENGINEERING JOURNAL

Combinatorial Approach for Exploring Conformational Space and Activation Barriers in Computer-Aided Enzyme Design

(2020) Dibyendu Mondal et al.   ACS Catalysis

Immunogenicity of Protein Therapeutics: A Lymph Node Perspective

(2020) Kristy Fu et al.   Frontiers in Immunology

Recent Strategies and Applications for l-Asparaginase Confinement

(2020) João C. F. Nunes et al.   MOLECULES

Molecular biology interventions for activity improvement and production of industrial enzymes

(2020) Shashi Kant Bhatia et al.   BIORESOURCE TECHNOLOGY

Production of a novel N-terminal PEGylated crisantaspase

(2019) Karin Torres-Obreque et al.   BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY

Novel site-specific PEGylated L-asparaginase

(2019) Giovanna Pastore Meneguetti et al.   PLoS One

Avoiding Proteolysis during the Extraction and Purification of Active Plant Enzymes

(2019) William C Plaxton   PLANT AND CELL PHYSIOLOGY

Acrylamide in human diet, its metabolism, toxicity, inactivation and the associated European Union legal regulations in food industry

(2019) Agnieszka Koszucka et al.   CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION

A structural in silico analysis of the immunogenicity of l-asparaginase from Escherichia coli and Erwinia carotovora

(2019) Lisandra Herrera Belén et al.   BIOLOGICALS

Bacterial l ‐asparaginases for cancer therapy: Current knowledge and future perspectives

(2019) Abdolmajid Ghasemian et al.   JOURNAL OF CELLULAR PHYSIOLOGY

Asparagine is a critical limiting metabolite for vaccinia virus protein synthesis during glutamine deprivation

(2019) Anil Pant et al.   JOURNAL OF VIROLOGY

L-Asparaginase from E. chrysanthemi expressed in glycoswitch®: effect of His-Tag fusion on the extracellular expression

(2019) Brian Effer et al.   PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY

Improvement of the activity of l-asparaginase I improvement of the catalytic activity of l-asparaginase I from Bacillus megaterium H-1 by in vitro directed evolution

(2019) Xiaohong Lu et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Antibodies Predict Pegaspargase Allergic Reactions and Failure of Rechallenge

(2019) Yiwei Liu et al.   JOURNAL OF CLINICAL ONCOLOGY

Decreasing the immunogenicity of Erwinia chrysanthemi asparaginase via protein engineering: computational approach

(2019) Maryam Yari et al.   MOLECULAR BIOLOGY REPORTS

A Review of the Microbial Production of Bioactive Natural Products and Biologics

(2019) Janette V. Pham et al.   Frontiers in Microbiology

Thermal sensitivity as a quality control attribute for biotherapeutics: The L‐asparaginase case

(2019) Han Yao et al.   Drug Testing and Analysis

What makes a good new therapeutic l-asparaginase?

(2019) Angela Beckett et al.   WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

Effect of chemical modification with carboxymethyl dextran on kinetic and structural properties of L-asparaginase

(2019) Marjan Chahardahcherik et al.   ANALYTICAL BIOCHEMISTRY

Prospects of PASylation® for the design of protein and peptide therapeutics with extended half-life and enhanced action

(2018) Michaela Gebauer et al.   BIOORGANIC & MEDICINAL CHEMISTRY

A Novel l-Asparaginase with low l-Glutaminase Coactivity Is Highly Efficacious against Both T- and B-cell Acute Lymphoblastic LeukemiasIn Vivo

(2018) Hien Anh Nguyen et al.   CANCER RESEARCH

As Extracellular Glutamine Levels Decline, Asparagine Becomes an Essential Amino Acid

(2018) Natalya N. Pavlova et al.   Cell Metabolism

Characterization of L-asparaginase from marine-derived Aspergillus niger AKV-MKBU, its antiproliferative activity and bench scale production using industrial waste

(2018) Anjana K. Vala et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Novel mutant of Escherichia coli asparaginase II to reduction of the glutaminase activity in treatment of acute lymphocytic leukemia by molecular dynamics simulations and QM-MM studies

(2018) Noeman Ardalan et al.   MEDICAL HYPOTHESES

Marine microbial L-asparaginase: Biochemistry, molecular approaches and applications in tumor therapy and in food industry

(2018) Fatemeh Izadpanah Qeshmi et al.   MICROBIOLOGICAL RESEARCH

Synthesis, characterization, crystal structure of novel bis-thiomethylcyclohexanone derivatives and their inhibitory properties against some metabolic enzymes

(2018) Abdullah Biçer et al.   BIOORGANIC CHEMISTRY

Drug-induced amino acid deprivation as strategy for cancer therapy

(2017) Marcus Kwong Lam Fung et al.   Journal of Hematology & Oncology

Acrylamide mitigation in French fries using native l-asparaginase from Aspergillus oryzae CCT 3940

(2017) Fernanda Furlan Gonçalves Dias et al.   LWT-FOOD SCIENCE AND TECHNOLOGY

Drug delivery: Brushing off antigenicity

(2017) Kang Choon Lee et al.   Nature Biomedical Engineering

Discovery of human-like L-asparaginases with potential clinical use by directed evolution

(2017) Coraline Rigouin et al.   Scientific Reports

Enhanced extracellular production of L-asparaginase from Bacillus subtilis 168 by B. subtilis WB600 through a combined strategy

(2016) Yue Feng et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Comparative immunogenicity and structural analysis of epitopes of different bacterial L-asparaginases

(2016) Vadim S. Pokrovsky et al.   BMC CANCER

Recent developments in l-asparaginase discovery and its potential as anticancer agent

(2016) Abhinav Shrivastava et al.   CRITICAL REVIEWS IN ONCOLOGY HEMATOLOGY

Enhanced catalysis of l-asparaginase from Bacillus licheniformis by a rational redesign

(2016) Ankit P. Sudhir et al.   ENZYME AND MICROBIAL TECHNOLOGY

Exendins and exendin analogs for diabetic therapy: a patent review (2012-2015)

(2016) Eun Ji Park et al.   EXPERT OPINION ON THERAPEUTIC PATENTS

Design and Characterization ofErwinia Chrysanthemil-Asparaginase Variants with Diminished l-Glutaminase Activity

(2016) Hien Anh Nguyen et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

The Modified Heparin-Binding l-Asparaginase of Wolinella succinogenes

(2016) E. P. Sannikova et al.   MOLECULAR BIOTECHNOLOGY

The Mystery of Antibodies Against Polyethylene Glycol (PEG) - What do we Know?

(2016) Christian Lubich et al.   PHARMACEUTICAL RESEARCH

Purification and Characterization of Glutaminase Free Asparaginase from Enterobacter cloacae: In-Vitro Evaluation of Cytotoxic Potential against Human Myeloid Leukemia HL-60 Cells

(2016) Islam Husain et al.   PLoS One

L-Asparaginase Purification

(2016) Louise L. Tundisi et al.   SEPARATION AND PURIFICATION REVIEWS

Recombinant L-asparaginase 1 from Saccharomyces cerevisiae: an allosteric enzyme with antineoplastic activity

(2016) Iris Munhoz Costa et al.   Scientific Reports

Studies on Deimmunization of Antileukaemic L-Asparaginase to have Reduced Clinical Immunogenicity- An in silico Approach

(2015) L. N. Ramya et al.   PATHOLOGY & ONCOLOGY RESEARCH

Asparaginase induces apoptosis and cytoprotective autophagy in chronic myeloid leukemia cells

(2015) Ping Song et al.   Oncotarget

Industrial effluent as a substrate for glutaminase free l-asparaginase production from Pseudomonas plecoglossicida strain RS1; media optimization, enzyme purification and its characterization

(2015) Ganeshan Shakambari et al.   RSC Advances

Cell or Cell Membrane-Based Drug Delivery Systems

(2015) Songwei Tan et al.   Theranostics

Cloning, expression and characterization of l-asparaginase from Pseudomonas fluorescens for large scale production in E. coli BL21

(2015) Vijay Kishore et al.   3 Biotech

Cloning, expression and characterization of l-asparaginase from Pseudomonas fluorescens for large scale production in E. coli BL21

(2015) Vijay Kishore et al.   3 Biotech

Engineered binding to erythrocytes induces immunological tolerance to E. coli asparaginase

(2015) K. M. Lorentz et al.   Science Advances

Engineering of Helicobacter pylori L-Asparaginase: Characterization of Two Functionally Distinct Groups of Mutants

(2015) Maristella Maggi et al.   PLoS One

Characterization of a Recombinant Glutaminase-Free l-Asparaginase (ansA3) Enzyme with High Catalytic Activity from Bacillus licheniformis

(2014) Ankit P. Sudhir et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Recent research progress on microbial l-asparaginases

(2014) Shaohua Zuo et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Improvement of stability and enzymatic activity by site-directed mutagenesis of E. coli asparaginase II

(2014) Shikha Verma et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

The glutaminase activity of L-asparaginase is not required for anticancer activity against ASNS-negative cells

(2014) W. K. Chan et al.   BLOOD

Cell-penetrating peptides meditated encapsulation of protein therapeutics into intact red blood cells and its application

(2014) Huining He et al.   JOURNAL OF CONTROLLED RELEASE

Synthesis and Carbonic Anhydrase Isoenzymes I, II, IX, and XII Inhibitory Effects of Dimethoxybromophenol Derivatives Incorporating Cyclopropane Moieties

(2014) Murat Boztaş et al.   JOURNAL OF MEDICINAL CHEMISTRY

Recombinant Deamidated Mutants of Erwinia chrysanthemi l-Asparaginase Have Similar or Increased Activity Compared to Wild-Type Enzyme

(2014) David Gervais et al.   MOLECULAR BIOTECHNOLOGY

Combination of environmental stress and localization of l-asparaginase in Arthrospira platensis for production improvement

(2014) Asep A. Prihanto et al.   3 Biotech

Asparaginase unveils glutamine-addicted AML

(2013) I. Samudio et al.   BLOOD

Glycosylation: impact, control and improvement during therapeutic protein production

(2013) Ana Rita Costa et al.   CRITICAL REVIEWS IN BIOTECHNOLOGY

Structure- and sequence-analysis inspired engineering of proteins for enhanced thermostability

(2013) Hein J Wijma et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Mutations in Subunit Interface and B-cell Epitopes Improve Antileukemic Activities ofEscherichia coliAsparaginase-II

(2013) Ranjit Kumar Mehta et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

PASylation: a biological alternative to PEGylation for extending the plasma half-life of pharmaceutically active proteins

(2013) M. Schlapschy et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Hyperthermophilic asparaginase mutants with enhanced substrate affinity and antineoplastic activity: structural insights on their mechanism of action

(2011) Saurabh Bansal et al.   FASEB JOURNAL

Engineering substrate specificity of E. carotovora l-asparaginase for the development of biosensor

(2011) Georgia A. Kotzia et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

In silico Engineering of L-Asparaginase to Have Reduced Glutaminase Side Activity for Effective Treatment of Acute Lymphoblastic Leukemia

(2011) Ramya LN et al.   JOURNAL OF PEDIATRIC HEMATOLOGY ONCOLOGY

Polyethylene Glycol-conjugated L-asparaginase Versus Native L-asparaginase in Combination With Standard Agents for Children With Acute Lymphoblastic Leukemia in Second Bone Marrow Relapse

(2011) Joanne Kurtzberg et al.   JOURNAL OF PEDIATRIC HEMATOLOGY ONCOLOGY

Engineering of glycosylation in yeast and other fungi: current state and perspectives

(2010) Karen De Pourcq et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Structural stability and functional analysis of L-asparaginase from Pyrococcus furiosus

(2010) S. Bansal et al.   BIOCHEMISTRY-MOSCOW

Glycosylation of Therapeutic Proteins

(2010) Ricardo J. Solá et al.   BIODRUGS

Purification and characterization of glutaminase-free l-asparaginase from Pectobacterium carotovorum MTCC 1428

(2010) Sanjay Kumar et al.   BIORESOURCE TECHNOLOGY

Rational engineering of L-asparaginase reveals importance of dual activity for cancer cell toxicity

(2010) M. N. Offman et al.   BLOOD

L-asparaginase treatment in acute lymphoblastic leukemia

(2010) Rob Pieters et al.   CANCER

Drug delivery by red blood cells: vascular carriers designed by mother nature

(2010) Vladimir R Muzykantov   Expert Opinion on Drug Delivery

Delivery of Therapeutic Proteins

(2010) Dipak S. Pisal et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Acrylamide and 5-hydroxymethylfurfural (HMF): A review on metabolism, toxicity, occurrence in food and mitigation strategies

(2010) Edoardo Capuano et al.   LWT-FOOD SCIENCE AND TECHNOLOGY

Design and fabrication of magnetic nanoparticles for targeted drug delivery and imaging

(2009) Omid Veiseh et al.   ADVANCED DRUG DELIVERY REVIEWS

Production of recombinant proteins by microbes and higher organisms

(2009) Arnold L. Demain et al.   BIOTECHNOLOGY ADVANCES

New Research Developments on Acrylamide: Analytical Chemistry, Formation Mechanism, and Mitigation Recipes

(2009) Yu Zhang et al.   CHEMICAL REVIEWS

Engineering thermal stability of l-asparaginase by in vitro directed evolution

(2009) Georgia A. Kotzia et al.   FEBS Journal

Engineering complex-type N-glycosylation in Pichia pastoris using GlycoSwitch technology

(2009) Pieter P Jacobs et al.   Nature Protocols

Clinical course and outcome in children with acute lymphoblastic leukemia and asparaginase-associated pancreatitis

(2009) Susan L. Kearney et al.   PEDIATRIC BLOOD & CANCER

ElliPro: a new structure-based tool for the prediction of antibody epitopes

(2008) Julia Ponomarenko et al.   BMC BIOINFORMATICS

(Re)defining biopharmaceutical

(2008) Ronald A Rader   NATURE BIOTECHNOLOGY