Challenges and progress towards industrial recombinant protein production in yeasts: A review

Innovative Bioprocess Strategies Combining Physiological Control and Strain Engineering of Pichia pastoris to Improve Recombinant Protein Production

(2022) Arnau Gasset et al.   Frontiers in Bioengineering and Biotechnology

Advancing Yarrowia lipolytica as a superior biomanufacturing platform by tuning gene expression using promoter engineering

(2022) Mei-Li Sun et al.   BIORESOURCE TECHNOLOGY

Biopharmaceutical benchmarks 2022

(2022) Gary Walsh et al.   NATURE BIOTECHNOLOGY

Current advances of Pichia pastoris as cell factories for production of recombinant proteins

(2022) Yingjie Pan et al.   Frontiers in Microbiology

Recent advances in systems and synthetic biology approaches for developing novel cell-factories in non-conventional yeasts

(2021) Pradipta Patra et al.   BIOTECHNOLOGY ADVANCES

Production of Industrial Enzymes via Pichia pastoris as a Cell Factory in Bioreactor: Current Status and Future Aspects

(2021) Zeynep Efsun Duman-Özdamar et al.   PROTEIN JOURNAL

Process development and scale-up optimization of the SARS-CoV-2 receptor binding domain–based vaccine candidate, RBD219-N1C1

(2021) Jungsoon Lee et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Bioprocess performance analysis of novel methanol-independent promoters for recombinant protein production with Pichia pastoris

(2021) Javier Garrigós-Martínez et al.   Microbial Cell Factories

Engineering of the unfolded protein response pathway in Pichia pastoris: enhancing production of secreted recombinant proteins

(2021) Hana Raschmanová et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Customized yeast cell factories for biopharmaceuticals: from cell engineering to process scale up

(2021) Aravind Madhavan et al.   Microbial Cell Factories

Characterization of methanol utilization negative Pichia pastoris for secreted protein production: New cultivation strategies for current and future applications

(2020) Domen Zavec et al.   BIOTECHNOLOGY AND BIOENGINEERING

Impact of oxygen availability onheterologous gene expression and polypeptide secretion dynamics in Yarrowia lipolytica ‐based protein production platforms

(2020) Maria Gorczyca et al.   YEAST

Kluyveromyces marxianus: An emerging yeast cell factory for applications in food and biotechnology

(2020) Ahasanul Karim et al.   INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY

Protein Glycoengineering: An Approach for Improving Protein Properties

(2020) Bo Ma et al.   Frontiers in Chemistry

Continuous Cultivation as a Tool Toward the Rational Bioprocess Development With Pichia Pastoris Cell Factory

(2020) Miguel Angel Nieto-Taype et al.   Frontiers in Bioengineering and Biotechnology

Understanding gradients in industrial bioreactors

(2020) Gisela Nadal-Rey et al.   BIOTECHNOLOGY ADVANCES

Yeast Expression Systems: Overview and Recent Advances

(2019) Roghayyeh Baghban et al.   MOLECULAR BIOTECHNOLOGY

A robust feeding control strategy adjusted and optimized by a neural network for enhancing of alpha 1-antitrypsin production in Pichia pastoris

(2019) Tina Tavasoli et al.   BIOCHEMICAL ENGINEERING JOURNAL

Elevated temperatures do not trigger a conserved metabolic network response among thermotolerant yeasts

(2019) Mathias Lehnen et al.   BMC MICROBIOLOGY

Continuous operation, a realistic alternative to fed-batch fermentation for the production of recombinant lipase B from Candida antarctica under the constitutive promoter PGK in Pichia pastoris

(2019) Julia de Macedo Robert et al.   BIOCHEMICAL ENGINEERING JOURNAL

Key determinants of global land-use projections

(2019) Elke Stehfest et al.   Nature Communications

Recombinant Proteins for Industrial versus Pharmaceutical Purposes: A Review of Process and Pricing

(2019) John Puetz et al.   Processes

Integration of comprehensive data and biotechnological tools for industrial applications of Kluyveromyces marxianus

(2019) Mochamad Nurcholis et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Physiological responses of Saccharomyces cerevisiae to industrially relevant conditions: slow growth, low pH and high CO 2 levels

(2019) Xavier Hakkaart et al.   BIOTECHNOLOGY AND BIOENGINEERING

Current advances in engineering tools for Pichia pastoris

(2019) Jasmin E Fischer et al.   CURRENT OPINION IN BIOTECHNOLOGY

Specific growth rate governs AOX1 gene expression, affecting the production kinetics of Pichia pastoris (Komagataella phaffii) PAOX1-driven recombinant producer strains with different target gene dosage

(2019) Javier Garrigós-Martínez et al.   Microbial Cell Factories

Contribution of the land sector to a 1.5 °C world

(2019) Stephanie Roe et al.   Nature Climate Change

Implementing CRISPR-Cas technologies in conventional and non-conventional yeasts: Current state and future prospects

(2018) Hana Raschmanová et al.   BIOTECHNOLOGY ADVANCES

Engineering strategies for enhanced production of protein and bio-products in Pichia pastoris : A review

(2018) Zhiliang Yang et al.   BIOTECHNOLOGY ADVANCES

Towards optimal substrate feeding for heterologous protein production in Pichia pastoris (Komagataella spp) fed-batch processes under P AOX1 control: a modeling aided approach

(2018) Xavier Ponte et al.   JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY

From industrial fermentor to CFD-guided downscaling: what have we learned?

(2018) Cees Haringa et al.   BIOCHEMICAL ENGINEERING JOURNAL

Improved secretory expression of lignocellulolytic enzymes in Kluyveromyces marxianus by promoter and signal sequence engineering

(2018) Jungang Zhou et al.   Biotechnology for Biofuels

Insight into the large-scale upstream fermentation environment using scaled-down models

(2018) Williams Olughu et al.   JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY

The Current Scientific and Regulatory Landscape in Advancing Integrated Continuous Biopharmaceutical Manufacturing

(2018) Adam C. Fisher et al.   TRENDS IN BIOTECHNOLOGY

Enhancing the efficiency of recombinant hepatitis B surface antigen production in Pichia pastoris by employing continuous fermentation

(2018) Alireza Rahimi et al.   BIOCHEMICAL ENGINEERING JOURNAL

Process development for the continuous production of heterologous proteins by the industrial yeast, Komagataella phaffii

(2018) Ayca Cankorur-Cetinkaya et al.   BIOTECHNOLOGY AND BIOENGINEERING

Saccharomyces cerevisiae morphological changes and cytokinesis arrest elicited by hypoxia during scale-up for production of therapeutic recombinant proteins

(2018) Juan C. Aon et al.   Microbial Cell Factories

The important versus the exciting: reining contradictions in contemporary biotechnology

(2018) Víctor de Lorenzo et al.   Microbial Biotechnology

A review of control strategies for manipulating the feed rate in fed-batch fermentation processes

(2017) Lisa Mears et al.   JOURNAL OF BIOTECHNOLOGY

Physiological state as transferable operating criterion to improve recombinant protein production in Pichia pastoris through oxygen limitation

(2017) Xavier Garcia-Ortega et al.   JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY

Identification of novel factors enhancing recombinant protein production in multi-copy Komagataella phaffii based on transcriptomic analysis of overexpression effects

(2017) Xiao-Wei Yu et al.   Scientific Reports

Single Cell Protein—State-of-the-Art, Industrial Landscape and Patents 2001–2016

(2017) Anneli Ritala et al.   Frontiers in Microbiology

Pichia pastoris Exhibits High Viability and a Low Maintenance Energy Requirement at Near-Zero Specific Growth Rates

(2016) Corinna Rebnegger et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Kluyveromyces marxianus as a host for heterologous protein synthesis

(2016) Andreas K. Gombert et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Dynamic behavior of Yarrowia lipolytica in response to pH perturbations: dependence of the stress response on the culture mode

(2016) Asma Timoumi et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Kluyveromyces lactis: An emerging tool in biotechnology

(2016) Sebastian C. Spohner et al.   JOURNAL OF BIOTECHNOLOGY

A step forward to improve recombinant protein production in Pichia pastoris : From specific growth rate effect on protein secretion to carbon-starving conditions as advanced strategy

(2016) Xavier Garcia-Ortega et al.   PROCESS BIOCHEMISTRY

Cultivation strategies to enhance productivity of Pichia pastoris: A review

(2015) V. Looser et al.   BIOTECHNOLOGY ADVANCES

On-line specific growth rate control for improving reduced glutathione production in Saccharomyces cerevisiae

(2015) Zhi-Qiang Xiong et al.   BIOTECHNOLOGY AND BIOPROCESS ENGINEERING

Scale-down of continuous protein producingSaccharomyces cerevisiaecultivations using a two-compartment system

(2015) Naia Risager Wright et al.   BIOTECHNOLOGY PROGRESS

The business impact of an integrated continuous biomanufacturing platform for recombinant protein production

(2015) Jason Walther et al.   JOURNAL OF BIOTECHNOLOGY

Microbial growth and physiology: a call for better craftsmanship

(2015) Thomas Egli   Frontiers in Microbiology

Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production

(2014) Mudassar Ahmad et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Combined Strategies for Improving the Production of Recombinant Rhizopus oryzae Lipase in Pichia pastoris

(2014) Xun Li et al.   BioResources

High-temperature cultivation of recombinant Pichia pastorisincreases endoplasmic reticulum stress and decreases production of human interleukin-10

(2014) Yongjun Zhong et al.   Microbial Cell Factories

Effect of oxygenation and temperature on glucose-xylose fermentation in Kluyveromyces marxianus CBS712 strain

(2014) Lorenzo Signori et al.   Microbial Cell Factories

Constitutive expression of alkaline β-mannanase in recombinant Pichia pastoris

(2014) Taicheng Zhu et al.   PROCESS BIOCHEMISTRY

Correlation of cell growth and heterologous protein production by Saccharomyces cerevisiae

(2013) Zihe Liu et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Investigation of the physiological response to oxygen limited process conditions of Pichia pastoris Mut+ strain using a two-compartment scale-down system

(2013) Bettina Lorantfy et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

A quantitative study of methanol/sorbitol co-feeding process of a Pichia pastoris Mut+/pAOX1-lacZ strain

(2013) Hongxing Niu et al.   Microbial Cell Factories

Constitutive Expression of Yarrowia lipolytica Lipase LIP2 in Pichia pastoris Using GAP as Promoter

(2012) Xiaofeng Wang et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Unconventional microbial systems for the cost-efficient production of high-quality protein therapeutics

(2012) José Luis Corchero et al.   BIOTECHNOLOGY ADVANCES

A dynamic fed batch strategy for a Pichia pastoris mixed feed system to increase process understanding

(2012) Dénes Zalai et al.   BIOTECHNOLOGY PROGRESS

Recombinant protein expression in Pichia pastoris strains with an engineered methanol utilization pathway

(2012) Florian W Krainer et al.   Microbial Cell Factories

Regulation of Pichia pastoris promoters and its consequences for protein production

(2012) Thomas Vogl et al.   New Biotechnology

Engineering Yarrowia lipolytica to Produce Glycoproteins Homogeneously Modified with the Universal Man3GlcNAc2 N-Glycan Core

(2012) Karen De Pourcq et al.   PLoS One

Improvement of Porcine Interferon-α Production by Recombinant Pichia pastoris via Induction at Low Methanol Concentration and Low Temperature

(2011) Hu Jin et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Enhanced recombinant human erythropoietin production by Pichia pastoris in methanol fed-batch/sorbitol batch fermentation through pH optimization

(2011) Elif Şükran Soyaslan et al.   BIOCHEMICAL ENGINEERING JOURNAL

Production of recombinant proteins by yeast cells

(2011) Eda Çelik et al.   BIOTECHNOLOGY ADVANCES

Optimization of a glycoengineered Pichia pastoris cultivation process for commercial antibody production

(2011) Jianxin Ye et al.   BIOTECHNOLOGY PROGRESS

The impact of oxygen on the transcriptome of recombinant S. cerevisiae and P. pastoris - a comparative analysis

(2011) Kristin Baumann et al.   BMC GENOMICS

Intracellular expression of Vitreoscilla hemoglobin improves production of Yarrowia lipolytica lipase LIP2 in a recombinant Pichia pastoris

(2011) Xiaofeng Wang et al.   ENZYME AND MICROBIAL TECHNOLOGY

Increasing recombinant protein production in Escherichia coli through metabolic and genetic engineering

(2011) Hendrik Waegeman et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Recombinant immunotherapeutics: current state and perspectives regarding the feasibility and market

(2010) Chung-Jr Huang et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Bioprocess engineering aspects of heterologous protein production in Pichia pastoris: A review

(2010) Gabriel Potvin et al.   BIOCHEMICAL ENGINEERING JOURNAL

Simple control of specific growth rate in biotechnological fed-batch processes based on enhanced online measurements of biomass

(2010) Michal Dabros et al.   BIOPROCESS AND BIOSYSTEMS ENGINEERING

Fermentation of lactose to bio-ethanol by yeasts as part of integrated solutions for the valorisation of cheese whey

(2010) Pedro M.R. Guimarães et al.   BIOTECHNOLOGY ADVANCES

Characterization of different promoters for designing a new expression vector in Saccharomyces cerevisiae

(2010) Siavash Partow et al.   YEAST

Kluyveromyces marxianus: A yeast emerging from its sister's shadow

(2010) Melanie M. Lane et al.   Fungal Biology Reviews

Influence of bioreactor hydraulic characteristics on a Saccharomyces cerevisiae fed-batch culture: hydrodynamic modelling and scale-down investigations

(2009) Annick Lejeune et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

The Effect of Temperature on the Proteome of RecombinantPichia pastoris

(2009) Martin Dragosits et al.   JOURNAL OF PROTEOME RESEARCH

Engineering complex-type N-glycosylation in Pichia pastoris using GlycoSwitch technology

(2009) Pieter P Jacobs et al.   Nature Protocols

Bioreactor scale-up and oxygen transfer rate in microbial processes: An overview

(2008) Felix Garcia-Ochoa et al.   BIOTECHNOLOGY ADVANCES

Feeding strategies for the enhanced production of recombinant human serum albumin in the fed-batch cultivation of Hansenula polymorpha

(2008) Joo-Hyung Heo et al.   PROCESS BIOCHEMISTRY

Pilot-scale fermentation, purification, and characterization of recombinant human Oncostatin M in Pichia pastoris

(2008) Ning Kong et al.   PROTEIN EXPRESSION AND PURIFICATION

Hypoxic fed-batch cultivation ofPichia pastoris increases specific and volumetric productivity of recombinant proteins

(2007) Kristin Baumann et al.   BIOTECHNOLOGY AND BIOENGINEERING