Downstream process and evaluation of the concomitant impact of a recombinant glycosylated L-asparaginase on leukemic cancer cells and the bone marrow tumor microenvironment

High Throughput Fluorescence-Based In Vitro Experimental Platform for the Identification of Effective Therapies to Overcome Tumour Microenvironment-Mediated Drug Resistance in AML

(2023) Yoana Arroyo-Berdugo et al.   Cancers

Predictive markers for efficiency of the amino-acid deprivation therapies in cancer

(2022) Vadim S. Pokrovsky et al.   Frontiers in Medicine

Adipocytes disrupt the translational programme of acute lymphoblastic leukaemia to favour tumour survival and persistence

(2021) Q. Heydt et al.   Nature Communications

ALL blasts drive primary mesenchymal stromal cells to increase asparagine availability during asparaginase treatment

(2021) Martina Chiu et al.   Blood Advances

Critical overview of the main features and techniques used for the evaluation of the clinical applicability of L-asparaginase as a biopharmaceutical to treat blood cancer

(2020) T.A. Costa-Silva et al.   BLOOD REVIEWS

Highly efficient Pyrococcus furiosus recombinant L-asparaginase with no glutaminase activity: Expression, purification, functional characterization, and cytotoxicity on THP-1, A549 and Caco-2 cell lines

(2020) Hesham Saeed et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Influence of lysosomal protease sensitivity in the immunogenicity of the antitumor biopharmaceutical asparaginase

(2020) Mariane A.D. Rodrigues et al.   BIOCHEMICAL PHARMACOLOGY

An improved method for simple and accurate colorimetric determination of l ‐asparaginase enzyme activity using Nessler's reagent

(2020) Rodrigo G Simas et al.   JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY

Production of a novel N-terminal PEGylated crisantaspase

(2019) Karin Torres-Obreque et al.   BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY

NG2 antigen is a therapeutic target for MLL-rearranged B-cell acute lymphoblastic leukemia

(2019) Belen Lopez-Millan et al.   LEUKEMIA

Acute myeloid leukaemia niche regulates response to L‐asparaginase

(2019) Ilaria M. Michelozzi et al.   BRITISH JOURNAL OF HAEMATOLOGY

L-Asparaginase from E. chrysanthemi expressed in glycoswitch®: effect of His-Tag fusion on the extracellular expression

(2019) Brian Effer et al.   PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY

Expression, purification, and characterization of asparaginase II from Saccharomyces cerevisiae in Escherichia coli

(2019) Wagner Lopes et al.   PROTEIN EXPRESSION AND PURIFICATION

Challenges for the Self-Assembly of Poly(Ethylene Glycol)–Poly(Lactic Acid) (PEG-PLA) into Polymersomes: Beyond the Theoretical Paradigms

(2018) Alexsandra Apolinário et al.   Nanomaterials

Low Bioavailability and High Immunogenicity of a New Brand of E. coli l -Asparaginase with Active Host Contaminating Proteins

(2018) Priscila Pini Zenatti et al.   EBioMedicine

Optimized upstream and downstream process conditions for the improved production of recombinant human asparaginase (rhASP) from Escherichia coli and its characterization

(2018) Rajesh Kumar Kante et al.   BIOLOGICALS

Targeting acute myeloid leukemia stem cell signaling by natural products

(2017) Kodappully Sivaraman Siveen et al.   Molecular Cancer

Identification of a cytogenetic and molecular subgroup of acute myeloid leukemias showing sensitivity to L-Asparaginase

(2017) Salvatore Nicola Bertuccio et al.   Oncotarget

Optimization, purification and characterization of recombinant L-asparaginase II in Escherichia coli

(2016) Thi Hien Nguyen Trang et al.   AFRICAN JOURNAL OF BIOTECHNOLOGY

Myeloid malignancies and the microenvironment

(2016) Claudia Korn et al.   BLOOD

Saccharomyces cerevisiae asparaginase II, a potential antileukemic drug: Purification and characterization of the enzyme expressed in Pichia pastoris

(2016) Luciana Facchinetti de Castro Girão et al.   PROTEIN EXPRESSION AND PURIFICATION

Inhibiting glutaminase in acute myeloid leukemia: metabolic dependency of selected AML subtypes

(2016) Polina Matre et al.   Oncotarget

Targeting glutaminolysis has antileukemic activity in acute myeloid leukemia and synergizes with BCL-2 inhibition

(2015) N. Jacque et al.   BLOOD

A germ line mutation in cathepsin B points toward a role in asparaginase pharmacokinetics

(2014) L. T. van der Meer et al.   BLOOD

Purification and Characterization of a Novel and Robust L-Asparaginase Having Low-Glutaminase Activity from Bacillus licheniformis: In Vitro Evaluation of Anti-Cancerous Properties

(2014) Richi V. Mahajan et al.   PLoS One

Inhibiting glutamine uptake represents an attractive new strategy for treating acute myeloid leukemia

(2013) L. Willems et al.   BLOOD

Structures of Apo and Product-Bound Human l-Asparaginase: Insights into the Mechanism of Autoproteolysis and Substrate Hydrolysis

(2012) Julian Nomme et al.   BIOCHEMISTRY

Fluorescence-based experimental model to evaluate the concomitant effect of drugs on the tumour microenvironment and cancer cells

(2012) Karthik Ramasamy et al.   BRITISH JOURNAL OF HAEMATOLOGY

Asparagine and aspartic acid concentrations in bone marrow versus peripheral blood during Berlin–Frankfurt–Münster-based induction therapy for childhood acute lymphoblastic leukemia

(2012) Manuel Steiner et al.   LEUKEMIA & LYMPHOMA

Elimination of  -mannose glycan structures in Pichia pastoris

(2011) D. Hopkins et al.   GLYCOBIOLOGY

Engineering of glycosylation in yeast and other fungi: current state and perspectives

(2010) Karen De Pourcq et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Tumor cell-specific bioluminescence platform to identify stroma-induced changes to anticancer drug activity

(2010) Douglas W McMillin et al.   NATURE MEDICINE

The effect of individual N-glycans on enzyme activity

(2009) Danielle Skropeta   BIOORGANIC & MEDICINAL CHEMISTRY