Bioinformatic analysis and genetic engineering approaches for recombinant biopharmaceutical glycoproteins production in microalgae

Phaeodactylum tricornutum: A Diatom Cell Factory

(2020) Thomas Butler et al.   TRENDS IN BIOTECHNOLOGY

Curcumin Derivative GT863 Inhibits Amyloid-Beta Production via Inhibition of Protein N-Glycosylation

(2020) Yasuomi Urano et al.   Cells

Perspectives for Glyco-Engineering of Recombinant Biopharmaceuticals from Microalgae

(2020) Lorenzo Barolo et al.   Cells

Functional characterization of poplar NAC13 gene in salt tolerance

(2019) Xuemei Zhang et al.   PLANT SCIENCE

Recombinant Proteins for Industrial versus Pharmaceutical Purposes: A Review of Process and Pricing

(2019) John Puetz et al.   Processes

Multiple xylosyltransferases heterogeneously xylosylate protein N ‐linked glycans in Chlamydomonas reinhardtii

(2019) Pierre‐Louis Lucas et al.   PLANT JOURNAL

Genome-wide investigation of WRKY gene family in pineapple: evolution and expression profiles during development and stress

(2018) Tao Xie et al.   BMC GENOMICS

Function of N -glycosylation in plants

(2018) Yukihiro Nagashima et al.   PLANT SCIENCE

Plant glyco-biotechnology

(2018) Jennifer Schoberer et al.   SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY

Genome-Wide Identification and Characterization of the Potato bHLH Transcription Factor Family

(2018) Ruoqiu Wang et al.   Genes

One-step generation of multiple gene knock-outs in the diatom Phaeodactylum tricornutum by DNA-free genome editing

(2018) Manuel Serif et al.   Nature Communications

Engineered Fusion Proteins for Efficient Protein Secretion and Purification of a Human Growth Factor from the Green Microalga Chlamydomonas reinhardtii

(2018) Thomas Baier et al.   ACS Synthetic Biology

Biological functions of fucose in mammals

(2017) Michael Schneider et al.   GLYCOBIOLOGY

Therapeutic glycoprotein production in mammalian cells

(2017) Marie-Eve Lalonde et al.   JOURNAL OF BIOTECHNOLOGY

From hybridomas to a robust microalgal-based production platform: molecular design of a diatom secreting monoclonal antibodies directed against the Marburg virus nucleoprotein

(2017) Franziska Hempel et al.   Microbial Cell Factories

Identification of methylated GnTI-dependent N -glycans in Botryococcus brauni

(2017) Stefan Schulze et al.   NEW PHYTOLOGIST

N-Glycoproteomic Characterization of Mannosidase and Xylosyltransferase Mutant Strains ofChlamydomonasreinhardtii

(2017) Stefan Schulze et al.   PLANT PHYSIOLOGY

Alga-Made Anti-Hepatitis B Antibody Binds to Human Fcγ Receptors

(2017) Gaëtan Vanier et al.   Biotechnology Journal

Towards autotrophic tissue engineering: Photosynthetic gene therapy for regeneration

(2016) Myra Noemi Chávez et al.   BIOMATERIALS

Biological roles of glycans

(2016) Ajit Varki   GLYCOBIOLOGY

Plant protein glycosylation

(2016) Richard Strasser   GLYCOBIOLOGY

Sialylation of N-glycans: mechanism, cellular compartmentalization and function

(2016) Gaurang P. Bhide et al.   HISTOCHEMISTRY AND CELL BIOLOGY

MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets

(2016) Sudhir Kumar et al.   MOLECULAR BIOLOGY AND EVOLUTION

Software tools for single-cell tracking and quantification of cellular and molecular properties

(2016) Oliver Hilsenbeck et al.   NATURE BIOTECHNOLOGY

Addressing various compartments of the diatom model organism Phaeodactylum tricornutum via sub-cellular marker proteins

(2016) Xiaojuan Liu et al.   Algal Research-Biomass Biofuels and Bioproducts

Production of Biopharmaceuticals in E. coli: Current Scenario and Future Perspectives

(2015) Mohammed N. Baeshen et al.   JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY

Using GlycoDelete to produce proteins lacking plant-specific N-glycan modification in seeds

(2015) Robin Piron et al.   NATURE BIOTECHNOLOGY

Review/N-glycans: The making of a varied toolbox

(2015) Nausicaä Lannoo et al.   PLANT SCIENCE

Genes Involved in the Endoplasmic Reticulum N-Glycosylation Pathway of the Red Microalga Porphyridium sp.: A Bioinformatic Study

(2014) Oshrat Levy-Ontman et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Biopharmaceutical benchmarks 2014

(2014) Gary Walsh   NATURE BIOTECHNOLOGY

Protein N-glycosylation in eukaryotic microalgae and its impact on the production of nuclear expressed biopharmaceuticals

(2014) Elodie Mathieu-Rivet et al.   Frontiers in Plant Science

Alga-Produced Cholera Toxin-Pfs25 Fusion Proteins as Oral Vaccines

(2013) James A. Gregory et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Ice recrystallization inhibition mediated by a nuclear-expressed and -secreted recombinant ice-binding protein in the microalga Chlamydomonas reinhardtii

(2013) Kyle J. Lauersen et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Preparation of transgenic Dunaliella salina for immunization against white spot syndrome virus in crayfish

(2013) Shuying Feng et al.   ARCHIVES OF VIROLOGY

nhmmer: DNA homology search with profile HMMs

(2013) T. J. Wheeler et al.   BIOINFORMATICS

Production of anti-cancer immunotoxins in algae: Ribosome inactivating proteins as fusion partners

(2013) Miller Tran et al.   BIOTECHNOLOGY AND BIOENGINEERING

Current state and recent advances in biopharmaceutical production in Escherichia coli, yeasts and mammalian cells

(2013) Aleš Berlec et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

A Chlamydomonas-Derived Human Papillomavirus 16 E7 Vaccine Induces Specific Tumor Protection

(2013) Olivia C. Demurtas et al.   PLoS One

Microbials for the production of monoclonal antibodies and antibody fragments

(2013) Oliver Spadiut et al.   TRENDS IN BIOTECHNOLOGY

Selenoprotein-Transgenic Chlamydomonas reinhardtii

(2013) Qintang Hou et al.   Nutrients

Heterologous expression of the C-terminal antigenic domain of the malaria vaccine candidate Pfs48/45 in the green algae Chlamydomonas reinhardtii

(2012) Carla S. Jones et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

The sweet tooth of biopharmaceuticals: Importance of recombinant protein glycosylation analysis

(2012) Nico Lingg et al.   Biotechnology Journal

Algae-Produced Pfs25 Elicits Antibodies That Inhibit Malaria Transmission

(2012) James A. Gregory et al.   PLoS One

Robust Expression and Secretion of Xylanase1 in Chlamydomonas reinhardtii by Fusion to a Selection Gene and Processing with the FMDV 2A Peptide

(2012) Beth A. Rasala et al.   PLoS One

Production of unique immunotoxin cancer therapeutics in algal chloroplasts

(2012) M. Tran et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Production of recombinant enzymes in the marine alga Dunaliella tertiolecta

(2012) D. Ryan Georgianna et al.   Algal Research-Biomass Biofuels and Bioproducts

Transgenic microalgae expressing Escherichia coli AppA phytase as feed additive to reduce phytate excretion in the manure of young broiler chicks

(2011) So-Mi Yoon et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

UniqueN-Glycan Moieties of the 66-kDa Cell Wall Glycoprotein from the Red MicroalgaPorphyridiumsp.

(2011) Oshrat Levy-Ontman et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Chemoenzymatic Synthesis and Fcγ Receptor Binding of Homogeneous Glycoforms of Antibody Fc Domain. Presence of a Bisecting Sugar Moiety Enhances the Affinity of Fc to FcγIIIa Receptor

(2011) Guozhang Zou et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Algae as Protein Factories: Expression of a Human Antibody and the Respective Antigen in the Diatom Phaeodactylum tricornutum

(2011) Franziska Hempel et al.   PLoS One

Green algae Chlamydomonas reinhardtii possess endogenous sialylated N-glycans

(2011) Tarlan Mamedov et al.   FEBS Open Bio

N-Glycans ofPhaeodactylum tricornutumDiatom and Functional Characterization of ItsN-Acetylglucosaminyltransferase I Enzyme

(2010) Bérengère Baïet et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Biopharmaceutical benchmarks 2010

(2010) Gary Walsh   NATURE BIOTECHNOLOGY

Production of therapeutic proteins in algae, analysis of expression of seven human proteins in the chloroplast of Chlamydomonas reinhardtii

(2010) Beth A. Rasala et al.   PLANT BIOTECHNOLOGY JOURNAL

Engineering the Chloroplast Targeted Malarial Vaccine Antigens in Chlamydomonas Starch Granules

(2010) David Dauvillée et al.   PLoS One

Towards revealing the structure of bacterial inclusion bodies

(2010) Lei Wang   Prion

Factors effecting expression of vaccines in microalgae

(2009) Raymond Surzycki et al.   BIOLOGICALS

Optimal and consistent protein glycosylation in mammalian cell culture

(2009) P. Hossler et al.   GLYCOBIOLOGY

Heat-stable oral alga-based vaccine protects mice from Staphylococcus aureus infection

(2009) Imke A.J. Dreesen et al.   JOURNAL OF BIOTECHNOLOGY

Strategies to facilitate transgene expression in Chlamydomonas reinhardtii

(2009) Alke Eichler-Stahlberg et al.   PLANTA

Improving intracellular production of recombinant protein in Pichia pastoris using an optimized preinduction glycerol-feeding scheme

(2008) Chun Wei et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

A novel expression platform for the production of diabetes-associated autoantigen human glutamic acid decarboxylase (hGAD65)

(2008) Xiaofeng Wang et al.   BMC BIOTECHNOLOGY

Protein Crystallography in Drug Discovery

(2008) Fernanda Canduri et al.   CURRENT DRUG TARGETS

Transgenic microalgae as a non-antibiotic bactericide producer to defend against bacterial pathogen infection in the fish digestive tract

(2008) Si-Shen Li et al.   FISH & SHELLFISH IMMUNOLOGY

Diversity in specificity, abundance, and composition of anti-Neu5Gc antibodies in normal humans: Potential implications for disease

(2008) V. Padler-Karavani et al.   GLYCOBIOLOGY

CONDITIONAL PRODUCTION OF A FUNCTIONAL FISH GROWTH HORMONE IN THE TRANSGENIC LINE OFNANNOCHLOROPSIS OCULATA(EUSTIGMATOPHYCEAE)

(2008) Hsin Liang Chen et al.   JOURNAL OF PHYCOLOGY

Cetuximab-Induced Anaphylaxis and IgE Specific for Galactose-α-1,3-Galactose

(2008) Christine H. Chung et al.   NEW ENGLAND JOURNAL OF MEDICINE