Highly efficient and simple SSPER and rrPCR approaches for the accurate site-directed mutagenesis of large and small plasmids

A high-efficiency method for site-directed mutagenesis of large plasmids based on large DNA fragment amplification and recombinational ligation

(2021) Kewei Zhang et al.   Scientific Reports

Improved designs for pET expression plasmids increase protein production yield in Escherichia coli

(2020) Patrick J. Shilling et al.   Communications Biology

In vivo DNA assembly using common laboratory bacteria: A re-emerging tool to simplify molecular cloning

(2019) Jake F. Watson et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

SDM-Assist software to design site-directed mutagenesis primers introducing “silent” restriction sites

(2013) Abhijit Karnik et al.   BMC BIOINFORMATICS

NIH Image to ImageJ: 25 years of image analysis

(2012) Caroline A Schneider et al.   NATURE METHODS

A Discussion of Molecular Biology Methods for Protein Engineering

(2011) Alexander Zawaira et al.   MOLECULAR BIOTECHNOLOGY

Simple and efficient site-directed mutagenesis using two single-primer reactions in parallel to generate mutants for protein structure-function studies

(2009) Oded Edelheit et al.   BMC BIOTECHNOLOGY

An easy-to-use site-directed mutagenesis method with a designed restriction site for convenient and reliable mutant screening

(2009) Bao-zhong Zhang et al.   Journal of Zhejiang University-SCIENCE B

A novel megaprimed and ligase-free, PCR-based, site-directed mutagenesis method

(2007) Wen-Chi Tseng et al.   ANALYTICAL BIOCHEMISTRY