Non-viral delivery of the CRISPR/Cas system: DNA versus RNA versus RNP

Gene Therapy for Drug-Resistant Glioblastoma via Lipid-Polymer Hybrid Nanoparticles Combined with Focused Ultrasound

(2021) Qiang Yang et al.   International Journal of Nanomedicine

Reprogramming the Tumor Microenvironment through Second‐Near‐Infrared‐Window Photothermal Genome Editing of PD‐L1 Mediated by Supramolecular Gold Nanorods for Enhanced Cancer Immunotherapy

(2021) Honglin Tang et al.   ADVANCED MATERIALS

Enhancing Gene Editing Efficiency for Cells by CRISPR/Cas9 System-Loaded Multilayered Nanoparticles Assembled via Microfluidics

(2021) Xuanyu Li et al.   CHINESE JOURNAL OF CHEMICAL ENGINEERING

Membrane-destabilizing ionizable phospholipids for organ-selective mRNA delivery and CRISPR–Cas gene editing

(2021) Shuai Liu et al.   NATURE MATERIALS

Lipid nanoparticle-mediated codelivery of Cas9 mRNA and single-guide RNA achieves liver-specific in vivo genome editing of Angptl3

(2021) Min Qiu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Microneedle-assisted genome editing: A transdermal strategy of targeting NLRP3 by CRISPR-Cas9 for synergistic therapy of inflammatory skin disorders

(2021) Tao Wan et al.   Science Advances

Co-encapsulation of Cas9 mRNA and guide RNA in polyplex micelles enables genome editing in mouse brain

(2021) Saed Abbasi et al.   JOURNAL OF CONTROLLED RELEASE

Charge-reversal nanocomolexes-based CRISPR/Cas9 delivery system for loss-of-function oncogene editing in hepatocellular carcinoma

(2021) Jing-Jun Nie et al.   JOURNAL OF CONTROLLED RELEASE

Delivery of cancer therapies by synthetic and bio-inspired nanovectors

(2021) Tina Briolay et al.   Molecular Cancer

Scaffold-mediated CRISPR-Cas9 delivery system for acute myeloid leukemia therapy

(2021) Tzu-Chieh Ho et al.   Science Advances

Engineered extracellular vesicles as versatile ribonucleoprotein delivery vehicles for efficient and safe CRISPR genome editing

(2021) Xingang Yao et al.   Journal of Extracellular Vesicles

All‐In‐One Dendrimer‐Based Lipid Nanoparticles Enable Precise HDR‐Mediated Gene Editing In Vivo

(2021) Lukas Farbiak et al.   ADVANCED MATERIALS

Traceable Nano‐Biohybrid Complexes by One‐Step Synthesis as CRISPR‐Chem Vectors for Neurodegenerative Diseases Synergistic Treatment

(2021) Jie Shen et al.   ADVANCED MATERIALS

Hypoxia‐Responsive Gene Editing to Reduce Tumor Thermal Tolerance for Mild‐Photothermal Therapy

(2021) Xueqing Li et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Highly Photoluminescent Nitrogen- and Zinc-Doped Carbon Dots for Efficient Delivery of CRISPR/Cas9 and mRNA

(2021) Akbar Hasanzadeh et al.   BIOCONJUGATE CHEMISTRY

One-in-one individual package and delivery of CRISPR/Cas9 ribonucleoprotein using apoferritin

(2021) Xiuhua Pan et al.   JOURNAL OF CONTROLLED RELEASE

In vivo targeted delivery of nucleic acids and CRISPR genome editors enabled by GSH-responsive silica nanoparticles

(2021) Yuyuan Wang et al.   JOURNAL OF CONTROLLED RELEASE

Versatile modification of the CRISPR/Cas9 ribonucleoprotein system to facilitate in vivo application

(2021) Bixi Sun et al.   JOURNAL OF CONTROLLED RELEASE

Synthesis and characterization of vitamin D3-functionalized carbon dots for CRISPR/Cas9 delivery

(2021) Akbar Hasanzadeh et al.   Nanomedicine

CRISPR-Cas9 In Vivo Gene Editing for Transthyretin Amyloidosis

(2021) Julian D. Gillmore et al.   NEW ENGLAND JOURNAL OF MEDICINE

Supramolecular Nanosubstrate‐Mediated Delivery for CRISPR/Cas9 Gene Disruption and Deletion

(2021) Qian Ban et al.   Small

Controlled CRISPR‐Cas9 Ribonucleoprotein Delivery for Sensitized Photothermal Therapy

(2021) Chao Chen et al.   Small

Targeted delivery of CRISPR-Cas9 and transgenes enables complex immune cell engineering

(2021) Jennifer R. Hamilton et al.   Cell Reports

Future considerations for the mRNA-lipid nanoparticle vaccine platform

(2021) Botond Z Igyártó et al.   Current Opinion in Virology

Rational Design of Poly(disulfide)s as a Universal Platform for Delivery of CRISPR-Cas9 Machineries toward Therapeutic Genome Editing

(2021) Jiajing Guo et al.   ACS Central Science

In vivo delivery of CRISPR-Cas9 therapeutics: Progress and challenges

(2021) Matthew Behr et al.   Acta Pharmaceutica Sinica B

Ionizable lipid-assisted efficient hepatic delivery of gene editing elements for oncotherapy

(2021) Chunhui Li et al.   Bioactive Materials

Delivery of Cas9/sgRNA Ribonucleoprotein Complexes via Hydroxystearyl Oligoamino Amides

(2020) Jasmin Kuhn et al.   BIOCONJUGATE CHEMISTRY

Ex vivo cell-based CRISPR/Cas9 genome editing for therapeutic applications

(2020) Yamin Li et al.   BIOMATERIALS

Efficient CRISPR/Cas9 Gene-chemo Synergistic Cancer Therapy via a Stimuli-Responsive Chitosan-Based Nanocomplex Elicits Anti-Tumorigenic Pathway Effect

(2020) Bingchen Zhang et al.   CHEMICAL ENGINEERING JOURNAL

Cell-Type-Specific CRISPR/Cas9 Delivery by Biomimetic Metal Organic Frameworks

(2020) Mram Z. Alyami et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Near-infrared optogenetic engineering of photothermal nanoCRISPR for programmable genome editing

(2020) Xiaohong Chen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Coupling Cas9 to artificial inhibitory domains enhances CRISPR-Cas9 target specificity

(2020) Sabine Aschenbrenner et al.   Science Advances

Opportunities and Challenges in the Delivery of mRNA-Based Vaccines

(2020) Abishek Wadhwa et al.   Pharmaceutics

Aptamer/Peptide‐Functionalized Genome‐Editing System for Effective Immune Restoration through Reversal of PD‐L1‐Mediated Cancer Immunosuppression

(2020) Xiao‐Yan He et al.   ADVANCED MATERIALS

The emerging role of RNA modifications in the regulation of mRNA stability

(2020) Sung Ho Boo et al.   EXPERIMENTAL AND MOLECULAR MEDICINE

Hyperbranched poly(β-amino ester) based polyplex nanopaticles for delivery of CRISPR/Cas9 system and treatment of HPV infection associated cervical cancer

(2020) Xueqin Gao et al.   JOURNAL OF CONTROLLED RELEASE

Engineered Interactions with Mesoporous Silica Facilitate Intracellular Delivery of Proteins and Gene Editing

(2020) Bin Liu et al.   NANO LETTERS

Safety and feasibility of CRISPR-edited T cells in patients with refractory non-small-cell lung cancer

(2020) You Lu et al.   NATURE MEDICINE

Selective organ targeting (SORT) nanoparticles for tissue-specific mRNA delivery and CRISPR–Cas gene editing

(2020) Qiang Cheng et al.   Nature Nanotechnology

Extracellular nanovesicles for packaging of CRISPR-Cas9 protein and sgRNA to induce therapeutic exon skipping

(2020) Peter Gee et al.   Nature Communications

Engineering of monosized lipid-coated mesoporous silica nanoparticles for CRISPR delivery

(2020) Achraf Noureddine et al.   Acta Biomaterialia

Phosphorothioate Modification of mRNA Accelerates the Rate of Translation Initiation to Provide More Efficient Protein Synthesis

(2020) Daisuke Kawaguchi et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Genome editing of mutant KRAS through supramolecular polymer-mediated delivery of Cas9 ribonucleoprotein for colorectal cancer therapy

(2020) Tao Wan et al.   JOURNAL OF CONTROLLED RELEASE

Light-responsive charge-reversal nanovector for high-efficiency in vivo CRISPR/Cas9 gene editing with controllable location and time

(2020) Yunxia Wu et al.   Nano Research

Poly(Beta-Amino Ester) Nanoparticles Enable Nonviral Delivery of CRISPR-Cas9 Plasmids for Gene Knockout and Gene Deletion

(2020) Yuan Rui et al.   Molecular Therapy-Nucleic Acids

A Lactose‐Derived CRISPR/Cas9 Delivery System for Efficient Genome Editing In Vivo to Treat Orthotopic Hepatocellular Carcinoma

(2020) Yu Qi et al.   Advanced Science

Appropriate Delivery of the CRISPR/Cas9 System through the Nonlysosomal Route: Application for Therapeutic Gene Editing

(2020) Hang Yin et al.   Advanced Science

Impact of mRNA chemistry and manufacturing process on innate immune activation

(2020) Jennifer Nelson et al.   Science Advances

Codelivery of CRISPR-Cas9 and chlorin e6 for spatially controlled tumor-specific gene editing with synergistic drug effects

(2020) Shaohui Deng et al.   Science Advances

An All-in-One Nanomedicine Consisting of CRISPR-Cas9 and an Autoantigen Peptide for Restoring Specific Immune Tolerance

(2020) Ying-Li Luo et al.   ACS Applied Materials & Interfaces

A Versatile Nonviral Delivery System for Multiplex Gene‐Editing in the Liver

(2020) Jing Gong et al.   ADVANCED MATERIALS

MicroRNA-responsive release of Cas9/sgRNA from DNA nanoflower for cytosolic protein delivery and enhanced genome editing

(2020) Jinjin Shi et al.   BIOMATERIALS

Tropism-facilitated delivery of CRISPR/Cas9 system with chimeric antigen receptor-extracellular vesicles against B-cell malignancies

(2020) Qian Xu et al.   JOURNAL OF CONTROLLED RELEASE

Extracellular vesicles engineered with valency-controlled DNA nanostructures deliver CRISPR/Cas9 system for gene therapy

(2020) Jialang Zhuang et al.   NUCLEIC ACIDS RESEARCH

mRNA Delivery Using Bioreducible Lipidoid Nanoparticles Facilitates Neural Differentiation of Human Mesenchymal Stem Cells

(2020) Xuewei Zhao et al.   Advanced Healthcare Materials

Co-delivery of Sorafenib and CRISPR/Cas9 Based on Targeted Core–Shell Hollow Mesoporous Organosilica Nanoparticles for Synergistic HCC Therapy

(2020) Bing-Chen Zhang et al.   ACS Applied Materials & Interfaces

Efficient Polymer-Mediated Delivery of Gene-Editing Ribonucleoprotein Payloads through Combinatorial Design, Parallelized Experimentation, and Machine Learning

(2020) Ramya Kumar et al.   ACS Nano

Recent advances in chemical modifications of guide RNA, mRNA and donor template for CRISPR-mediated genome editing

(2020) Qiubing Chen et al.   ADVANCED DRUG DELIVERY REVIEWS

Hierarchical Self‐assembly of Discrete Metal–Organic Cages into Supramolecular Nanoparticles for Intracellular Protein Delivery

(2020) Ji Liu et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Binding‐mediated formation of ribonucleoprotein corona for efficient delivery and control of CRISPR‐Cas9

(2020) Jinjun Wu et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Delivery of CRISPR/Cas9 Plasmids by Cationic Gold Nanorods: Impact of the Aspect Ratio on Genome Editing and Treatment of Hepatic Fibrosis

(2020) Yuxuan Chen et al.   CHEMISTRY OF MATERIALS

In vitro transcribed sgRNA causes cell death by inducing interferon release

(2019) Wei Mu et al.   Protein & Cell

Antibody-targeted chromatin enables effective intracellular delivery and functionality of CRISPR/Cas9 expression plasmids

(2019) Tobias Killian et al.   NUCLEIC ACIDS RESEARCH

In vivo neuronal gene editing via CRISPR–Cas9 amphiphilic nanocomplexes alleviates deficits in mouse models of Alzheimer’s disease

(2019) Hanseul Park et al.   NATURE NEUROSCIENCE

Identification of preexisting adaptive immunity to Cas9 proteins in humans

(2019) Carsten T. Charlesworth et al.   NATURE MEDICINE

Delivering the Messenger: Advances in Technologies for Therapeutic mRNA Delivery

(2019) Piotr S. Kowalski et al.   MOLECULAR THERAPY

Nanoscale ATP-Responsive Zeolitic Imidazole Framework-90 as a General Platform for Cytosolic Protein Delivery and Genome Editing

(2019) Xiaoti Yang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

CRISPR to fix bad blood: a new tool in basic and clinical hematology

(2019) Elisa González-Romero et al.   HAEMATOLOGICA

Microenvironment-Responsive Delivery of the Cas9 RNA-Guided Endonuclease for Efficient Genome Editing

(2019) Jun Yin et al.   BIOCONJUGATE CHEMISTRY

Chitosan for gene delivery: Methods for improvement and applications

(2019) Di Chuan et al.   ADVANCES IN COLLOID AND INTERFACE SCIENCE

Reducible Branched Ester-Amine Quadpolymers (rBEAQs) Codelivering Plasmid DNA and RNA Oligonucleotides Enable CRISPR/Cas9 Genome Editing

(2019) Yuan Rui et al.   ACS Applied Materials & Interfaces

A Rationally Designed Semiconducting Polymer Brush for NIR‐II Imaging‐Guided Light‐Triggered Remote Control of CRISPR/Cas9 Genome Editing

(2019) Ling Li et al.   ADVANCED MATERIALS

Targeted homology-directed repair in blood stem and progenitor cells with CRISPR nanoformulations

(2019) Reza Shahbazi et al.   NATURE MATERIALS

CRISPR/Cas9 Delivery Mediated with Hydroxyl‐Rich Nanosystems for Gene Editing in Aorta

(2019) Xiaoping Zhang et al.   Advanced Science

Near-infrared upconversion–activated CRISPR-Cas9 system: A remote-controlled gene editing platform

(2019) Yongchun Pan et al.   Science Advances

Peptide and Aptamer Decorated Delivery System for Targeting Delivery of Cas9/sgRNA Plasmid To Mediate Antitumor Genome Editing

(2019) Bo-Ya Liu et al.   ACS Applied Materials & Interfaces

Fast and Efficient CRISPR/Cas9 Genome Editing In Vivo Enabled by Bioreducible Lipid and Messenger RNA Nanoparticles

(2019) Ji Liu et al.   ADVANCED MATERIALS

Triple-Targeting Delivery of CRISPR/Cas9 to Reduce the Risk of Cardiovascular Diseases

(2019) Lingmin Zhang et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Knockdown of hypoxia-inducible factor-1 alpha by tumor targeted delivery of CRISPR/Cas9 system suppressed the metastasis of pancreatic cancer

(2019) Man Li et al.   JOURNAL OF CONTROLLED RELEASE

A boronic acid–rich dendrimer with robust and unprecedented efficiency for cytosolic protein delivery and CRISPR-Cas9 gene editing

(2019) Chongyi Liu et al.   Science Advances

Cdk5 knocking out mediated by CRISPR-Cas9 genome editing for PD-L1 attenuation and enhanced antitumor immunity

(2019) Huan Deng et al.   Acta Pharmaceutica Sinica B

Active fusions of Cas9 orthologs

(2019) Goran Josipović et al.   JOURNAL OF BIOTECHNOLOGY

A biodegradable nanocapsule delivers a Cas9 ribonucleoprotein complex for in vivo genome editing

(2019) Guojun Chen et al.   Nature Nanotechnology

Cell-Selective Messenger RNA Delivery and CRISPR/Cas9 Genome Editing by Modulating the Interface of Phenylboronic Acid-Derived Lipid Nanoparticles and Cellular Surface Sialic Acid

(2019) Qiao Tang et al.   ACS Applied Materials & Interfaces

Rational designs of in vivo CRISPR-Cas delivery systems

(2019) Cong-Fei Xu et al.   ADVANCED DRUG DELIVERY REVIEWS

Simple and Efficient Targeted Intracellular Protein Delivery with Self‐Assembled Nanovehicles for Effective Cancer Therapy

(2019) Xiao He et al.   ADVANCED FUNCTIONAL MATERIALS

A Photolabile Semiconducting Polymer Nanotransducer for Near-Infrared Regulation of CRISPR/Cas9 Gene Editing

(2019) Yan Lyu et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

In situ repurposing of dendritic cells with CRISPR/Cas9-based nanomedicine to induce transplant tolerance

(2019) Yue Zhang et al.   BIOMATERIALS

A Self-Assembled Platform Based on Branched DNA for sgRNA/Cas9/Antisense Delivery

(2019) Jianbing Liu et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

NanoRNP Overcomes Tumor Heterogeneity in Cancer Treatment

(2019) Qi Liu et al.   NANO LETTERS

Lipopeptide‐Based Nanosome‐Mediated Delivery of Hyperaccurate CRISPR/Cas9 Ribonucleoprotein for Gene Editing

(2019) Trung Thanh Thach et al.   Small

Engineered amphiphilic peptides enable delivery of proteins and CRISPR-associated nucleases to airway epithelia

(2019) Sateesh Krishnamurthy et al.   Nature Communications

Ultrasound-activated particles as CRISPR/Cas9 delivery system for androgenic alopecia therapy

(2019) Jee-Yeon Ryu et al.   BIOMATERIALS

Carboxylated branched poly(β-amino ester) nanoparticles enable robust cytosolic protein delivery and CRISPR-Cas9 gene editing

(2019) Yuan Rui et al.   Science Advances

Macrophage-Specific in Vivo Gene Editing Using Cationic Lipid-Assisted Polymeric Nanoparticles

(2018) Ying-Li Luo et al.   ACS Nano

Simple In Vivo Gene Editing via Direct Self-Assembly of Cas9 Ribonucleoprotein Complexes for Cancer Treatment

(2018) Seung Min Kim et al.   ACS Nano

Active Intracellular Delivery of a Cas9/sgRNA Complex Using Ultrasound-Propelled Nanomotors

(2018) Malthe Hansen-Bruhn et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

A Dual-Targeting Delivery System for Effective Genome Editing and In Situ Detecting Related Protein Expression in Edited Cells

(2018) Bo-Ya Liu et al.   BIOMACROMOLECULES

Optimization of lipid-assisted nanoparticle for disturbing neutrophils-related inflammation

(2018) Yang Liu et al.   BIOMATERIALS

Emergence of synthetic mRNA: In vitro synthesis of mRNA and its applications in regenerative medicine

(2018) Hyokyoung Kwon et al.   BIOMATERIALS

CRISPR RNAs trigger innate immune responses in human cells

(2018) Sojung Kim et al.   GENOME RESEARCH

Receptor-Mediated Delivery of CRISPR-Cas9 Endonuclease for Cell-Type-Specific Gene Editing

(2018) Romain Rouet et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Cationic Polymer-Mediated CRISPR/Cas9 Plasmid Delivery for Genome Editing

(2018) Zhen Zhang et al.   MACROMOLECULAR RAPID COMMUNICATIONS

Effective PEI-mediated delivery of CRISPR-Cas9 complex for targeted gene therapy

(2018) Nari Ryu et al.   Nanomedicine-Nanotechnology Biology and Medicine

Graphene oxide-mediated Cas9/sgRNA delivery for efficient genome editing

(2018) Huahua Yue et al.   Nanoscale

A highly specific SpCas9 variant is identified by in vivo screening in yeast

(2018) Antonio Casini et al.   NATURE BIOTECHNOLOGY

mRNA vaccines — a new era in vaccinology

(2018) Norbert Pardi et al.   NATURE REVIEWS DRUG DISCOVERY

Nonviral gene editing via CRISPR/Cas9 delivery by membrane-disruptive and endosomolytic helical polypeptide

(2018) Hong-Xia Wang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Heavily and fully modified RNAs guide efficient SpyCas9-mediated genome editing

(2018) Aamir Mir et al.   Nature Communications

A Single Administration of CRISPR/Cas9 Lipid Nanoparticles Achieves Robust and Persistent In Vivo Genome Editing

(2018) Jonathan D. Finn et al.   Cell Reports

In vitro–transcribed guide RNAs trigger an innate immune response via the RIG-I pathway

(2018) Beeke Wienert et al.   PLOS BIOLOGY

Systemic delivery of CRISPR/Cas9 with PEG-PLGA nanoparticles for chronic myeloid leukemia targeted therapy

(2018) Yang Liu et al.   Biomaterials Science

VSV-G-Enveloped Vesicles for Traceless Delivery of CRISPR-Cas9

(2018) Claudia Montagna et al.   Molecular Therapy-Nucleic Acids

Uridine Depletion and Chemical Modification Increase Cas9 mRNA Activity and Reduce Immunogenicity without HPLC Purification

(2018) Sriram Vaidyanathan et al.   Molecular Therapy-Nucleic Acids

Exosome-Liposome Hybrid Nanoparticles Deliver CRISPR/Cas9 System in MSCs

(2018) Yao Lin et al.   Advanced Science

HPV Oncogene Manipulation Using Nonvirally Delivered CRISPR/Cas9 or Natronobacterium gregoryi Argonaute

(2018) Yeh-Hsing Lao et al.   Advanced Science

Prevalence of Pre-existing Antibodies to CRISPR-Associated Nuclease Cas9 in the USA Population

(2018) Vijaya L. Simhadri et al.   Molecular Therapy-Methods & Clinical Development

PEGylated Chitosan for Non-Viral Aerosol and Mucosal Delivery of CRISPR-Cas9 System in Vitro

(2018) Hairui Zhang et al.   MOLECULAR PHARMACEUTICS

Optimized nanoparticle-mediated delivery of CRISPR-Cas9 system for B cell intervention

(2018) Min Li et al.   Nano Research

Evolved Cas9 variants with broad PAM compatibility and high DNA specificity

(2018) Johnny H. Hu et al.   NATURE

Fluorinated Acid-Labile Branched Hydroxyl-Rich Nanosystems for Flexible and Robust Delivery of Plasmids

(2018) Yu Qi et al.   Small

Directed evolution of CRISPR-Cas9 to increase its specificity

(2018) Jungjoon K. Lee et al.   Nature Communications

Cas9 immunity creates challenges for CRISPR gene editing therapies

(2018) Julie M. Crudele et al.   Nature Communications

Protein Engineering Strategies to Expand CRISPR-Cas9 Applications

(2018) Lucas F. Ribeiro et al.   International Journal of Genomics

Synthesis and Evaluation of pH-Sensitive Multifunctional Lipids for Efficient Delivery of CRISPR/Cas9 in Gene Editing

(2018) Da Sun et al.   BIOCONJUGATE CHEMISTRY

Stearyl polyethylenimine complexed with plasmids as the core of human serum albumin nanoparticles noncovalently bound to CRISPR/Cas9 plasmids or siRNA for disrupting or silencing PD-L1 expression for immunotherapy

(2018) Wei-Jie Cheng et al.   International Journal of Nanomedicine

Potent Protein Delivery into Mammalian Cells via a Supercharged Polypeptide

(2018) Jun Yin et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Gesicle-Mediated Delivery of CRISPR/Cas9 Ribonucleoprotein Complex for Inactivating the HIV Provirus

(2018) Lee A. Campbell et al.   MOLECULAR THERAPY

Genome editing in primary cells and in vivo using viral-derived Nanoblades loaded with Cas9-sgRNA ribonucleoproteins

(2018) Philippe E. Mangeot et al.   Nature Communications

Shortening the Half-Life of Cas9 Maintains Its Gene Editing Ability and Reduces Neuronal Toxicity

(2018) Su Yang et al.   Cell Reports

Minicircle Versus Plasmid DNA Delivery by Receptor-Targeted Polyplexes

(2017) Ana Krhac Levacic et al.   HUMAN GENE THERAPY

Cancer-derived exosomes as a delivery platform of CRISPR/Cas9 confer cancer cell tropism-dependent targeting

(2017) Seung Min Kim et al.   JOURNAL OF CONTROLLED RELEASE

Combining reactive triblock copolymers with functional cross-linkers: A versatile pathway to disulfide stabilized-polyplex libraries and their application as pDNA vaccines

(2017) Philipp Heller et al.   JOURNAL OF CONTROLLED RELEASE

Endosomal Escape and Delivery of CRISPR/Cas9 Genome Editing Machinery Enabled by Nanoscale Zeolitic Imidazolate Framework

(2017) Shahad K. Alsaiari et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Enhanced proofreading governs CRISPR–Cas9 targeting accuracy

(2017) Janice S. Chen et al.   NATURE

Structure-guided chemical modification of guide RNA enables potent non-viral in vivo genome editing

(2017) Hao Yin et al.   NATURE BIOTECHNOLOGY

Improving CRISPR–Cas specificity with chemical modifications in single-guide RNAs

(2017) Daniel E Ryan et al.   NUCLEIC ACIDS RESEARCH

Monitoring integrity and localization of modified single-stranded RNA oligonucleotides using ultrasensitive fluorescence methods

(2017) Philipp Heissig et al.   PLoS One

Non-viral and viral delivery systems for CRISPR-Cas9 technology in the biomedical field

(2017) Zhi-Yao He et al.   Science China-Life Sciences

Peptide/Cas9 nanostructures for ribonucleoprotein cell membrane transport and gene edition

(2017) Irene Lostalé-Seijo et al.   Chemical Science

Nanoparticle delivery of Cas9 ribonucleoprotein and donor DNA in vivo induces homology-directed DNA repair

(2017) Kunwoo Lee et al.   Nature Biomedical Engineering

Minimal 2'-O-methyl phosphorothioate linkage modification pattern of synthetic guide RNAs for increased stability and efficient CRISPR-Cas9 gene editing avoiding cellular toxicity

(2017) Megan Basila et al.   PLoS One

Promoting Cas9 degradation reduces mosaic mutations in non-human primate embryos

(2017) Zhuchi Tu et al.   Scientific Reports

Approach To Deliver Two Antioxidant Enzymes with Mesoporous Silica Nanoparticles into Cells

(2016) Yu-Hsuan Lin et al.   ACS Applied Materials & Interfaces

Artificial Virus Delivers CRISPR-Cas9 System for Genome Editing of Cells in Mice

(2016) Ling Li et al.   ACS Nano

A Single Methylene Group in Oligoalkylamine-Based Cationic Polymers and Lipids Promotes Enhanced mRNA Delivery

(2016) Anita Jarzębińska et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Evading innate immunity in nonviral mRNA delivery: don’t shoot the messenger

(2016) Joke Devoldere et al.   DRUG DISCOVERY TODAY

Acid-labile pHPMA modification of four-arm oligoaminoamide pDNA polyplexes balances shielding and gene transfer activity in vitro and in vivo

(2016) Linda Beckert et al.   EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS

CRISPRs for Optimal Targeting: Delivery of CRISPR Components as DNA, RNA, and Protein into Cultured Cells and Single-Cell Embryos

(2016) Evguenia Kouranova et al.   HUMAN GENE THERAPY

Recognition of Endogenous Nucleic Acids by the Innate Immune System

(2016) Axel Roers et al.   IMMUNITY

Toward Artificial Immunotoxins: Traceless Reversible Conjugation of RNase A with Receptor Targeting and Endosomal Escape Domains

(2016) Xiaowen Liu et al.   MOLECULAR PHARMACEUTICS

130. Purification of Large Scale mRNA Encoding ZFN Nucleases by dHPLC Technology

(2016) Erica Giuliani et al.   MOLECULAR THERAPY

High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects

(2016) Benjamin P. Kleinstiver et al.   NATURE

Therapeutic genome editing by combined viral and non-viral delivery of CRISPR system components in vivo

(2016) Hao Yin et al.   NATURE BIOTECHNOLOGY

Systematic study of the dynamics and half-lives of newly synthesized proteins in human cells

(2016) Weixuan Chen et al.   Chemical Science

Rapid and highly efficient mammalian cell engineering via Cas9 protein transfection

(2015) Xiquan Liang et al.   JOURNAL OF BIOTECHNOLOGY

Stability analysis of chemically modified mRNA using micropattern-based single-cell arrays

(2015) Mehrije Ferizi et al.   LAB ON A CHIP

Sequence-engineered mRNA Without Chemical Nucleoside Modifications Enables an Effective Protein Therapy in Large Animals

(2015) Andreas Thess et al.   MOLECULAR THERAPY

Synthesis and applications of RNAs with position-selective labelling and mosaic composition

(2015) Yu Liu et al.   NATURE

Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells

(2015) Ayal Hendel et al.   NATURE BIOTECHNOLOGY

A split-Cas9 architecture for inducible genome editing and transcription modulation

(2015) Bernd Zetsche et al.   NATURE BIOTECHNOLOGY

Photoactivatable CRISPR-Cas9 for optogenetic genome editing

(2015) Yuta Nihongaki et al.   NATURE BIOTECHNOLOGY

Small molecule–triggered Cas9 protein with improved genome-editing specificity

(2015) Kevin M Davis et al.   Nature Chemical Biology

Discovery of m7G-cap in eukaryotic mRNAs

(2015) Yasuhiro FURUICHI   PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES

Synthetic CRISPR RNA-Cas9–guided genome editing in human cells

(2015) Meghdad Rahdar et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Rationally engineered Cas9 nucleases with improved specificity

(2015) I. M. Slaymaker et al.   SCIENCE

Covalent Attachment of Cyclic TAT Peptides to GFP Results in Protein Delivery into Live Cells with Immediate Bioavailability

(2014) Nicole Nischan et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

mRNA transcript therapy

(2014) Drew Weissman   Expert Review of Vaccines

Gene disruption by cell-penetrating peptide-mediated delivery of Cas9 protein and guide RNA

(2014) S. Ramakrishna et al.   GENOME RESEARCH

Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins

(2014) S. Kim et al.   GENOME RESEARCH

Cell division responsive peptides for optimized plasmid DNA delivery: The mitotic window of opportunity?

(2014) K. Remaut et al.   JOURNAL OF CONTROLLED RELEASE

Modulated Protonation of Side Chain Aminoethylene Repeats in N-Substituted Polyaspartamides Promotes mRNA Transfection

(2014) Hirokuni Uchida et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo

(2014) John A Zuris et al.   NATURE BIOTECHNOLOGY

CRISPR-Cas systems for editing, regulating and targeting genomes

(2014) Jeffry D Sander et al.   NATURE BIOTECHNOLOGY

Fusion of catalytically inactive Cas9 to FokI nuclease improves the specificity of genome modification

(2014) John P Guilinger et al.   NATURE BIOTECHNOLOGY

mRNA-based therapeutics — developing a new class of drugs

(2014) Ugur Sahin et al.   NATURE REVIEWS DRUG DISCOVERY

The new frontier of genome engineering with CRISPR-Cas9

(2014) J. A. Doudna et al.   SCIENCE

Structures of Cas9 Endonucleases Reveal RNA-Mediated Conformational Activation

(2014) M. Jinek et al.   SCIENCE

Direct Delivery of Functional Proteins and Enzymes to the Cytosol Using Nanoparticle-Stabilized Nanocapsules

(2013) Rui Tang et al.   ACS Nano

Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity

(2013) F. Ann Ran et al.   CELL

Single-cell mRNA transfection studies: Delivery, kinetics and statistics by numbers

(2013) Carolin Leonhardt et al.   Nanomedicine-Nanotechnology Biology and Medicine

Fine-tuning of proton sponges by precise diaminoethanes and histidines in pDNA polyplexes

(2013) Ulrich Lächelt et al.   Nanomedicine-Nanotechnology Biology and Medicine

Rewriting a genome

(2013) Emmanuelle Charpentier et al.   NATURE

Safety and Efficacy of RNAi Therapy for Transthyretin Amyloidosis

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