Protein Engineering Strategies to Expand CRISPR-Cas9 Applications

Evolved Cas9 variants with broad PAM compatibility and high DNA specificity

(2018) Johnny H. Hu et al.   NATURE

Mutations in Cas9 Enhance the Rate of Acquisition of Viral Spacer Sequences during the CRISPR-Cas Immune Response

(2017) Robert Heler et al.   MOLECULAR CELL

Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage

(2017) Nicole M. Gaudelli et al.   NATURE

Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions

(2017) Y Bill Kim et al.   NATURE BIOTECHNOLOGY

What rheumatologists need to know about CRISPR/Cas9

(2017) Gary J. Gibson et al.   Nature Reviews Rheumatology

Optical control of cell signaling by single-chain photoswitchable kinases

(2017) Xin X. Zhou et al.   SCIENCE

Programmable DNA looping using engineered bivalent dCas9 complexes

(2017) Nan Hao et al.   Nature Communications

Manipulation of nuclear architecture through CRISPR-mediated chromosomal looping

(2017) Stefanie L. Morgan et al.   Nature Communications

Improving the DNA specificity and applicability of base editing through protein engineering and protein delivery

(2017) Holly A. Rees et al.   Nature Communications

A decade of discovery: CRISPR functions and applications

(2017) Rodolphe Barrangou et al.   Nature Microbiology

Targeted DNA methylation in human cells using engineered dCas9-methyltransferases

(2017) Tina Xiong et al.   Scientific Reports

A xylose-stimulated xylanase–xylose binding protein chimera created by random nonhomologous recombination

(2016) Lucas Ferreira Ribeiro et al.   Biotechnology for Biofuels

Inheritable Silencing of Endogenous Genes by Hit-and-Run Targeted Epigenetic Editing

(2016) Angelo Amabile et al.   CELL

The discovery of CRISPR in archaea and bacteria

(2016) Francisco J. M. Mojica et al.   FEBS Journal

Rewiring Cas9 to Target New PAM Sequences

(2016) Virginijus Siksnys et al.   MOLECULAR CELL

New CRISPR–Cas systems from uncultivated microbes

(2016) David Burstein et al.   NATURE

Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage

(2016) Alexis C. Komor et al.   NATURE

High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects

(2016) Benjamin P. Kleinstiver et al.   NATURE

Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch

(2016) Benjamin L Oakes et al.   NATURE BIOTECHNOLOGY

Applications of CRISPR technologies in research and beyond

(2016) Rodolphe Barrangou et al.   NATURE BIOTECHNOLOGY

Multidimensional chemical control of CRISPR–Cas9

(2016) Basudeb Maji et al.   Nature Chemical Biology

A chemical-inducible CRISPR–Cas9 system for rapid control of genome editing

(2016) Kaiwen Ivy Liu et al.   Nature Chemical Biology

Complex transcriptional modulation with orthogonal and inducible dCas9 regulators

(2016) Yuchen Gao et al.   NATURE METHODS

Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells

(2016) Gaelen T Hess et al.   NATURE METHODS

Defining and improving the genome-wide specificities of CRISPR–Cas9 nucleases

(2016) Shengdar Q. Tsai et al.   NATURE REVIEWS GENETICS

Engineering of temperature- and light-switchable Cas9 variants

(2016) Florian Richter et al.   NUCLEIC ACIDS RESEARCH

Guide RNA engineering for versatile Cas9 functionality

(2016) Chance M. Nowak et al.   NUCLEIC ACIDS RESEARCH

Efficient targeted DNA methylation with chimeric dCas9–Dnmt3a–Dnmt3L methyltransferase

(2016) Peter Stepper et al.   NUCLEIC ACIDS RESEARCH

Repurposing the CRISPR-Cas9 system for targeted DNA methylation

(2016) Aleksandar Vojta et al.   NUCLEIC ACIDS RESEARCH

On the Origin of CRISPR-Cas Technology: From Prokaryotes to Mammals

(2016) Francisco J.M. Mojica et al.   TRENDS IN MICROBIOLOGY

Expressing Transgenes That Exceed the Packaging Capacity of Adeno-Associated Virus Capsids

(2016) Kyle Chamberlain et al.   Human Gene Therapy Methods

Reprogrammable CRISPR/Cas9-based system for inducing site-specific DNA methylation

(2016) James I. McDonald et al.   Biology Open

The CRISPR-Cas immune system: Biology, mechanisms and applications

(2015) Devashish Rath et al.   BIOCHIMIE

Enzymatic protein switches built from paralogous input domains

(2015) Jennifer Tullman et al.   BIOTECHNOLOGY AND BIOENGINEERING

Insertion of a xylanase in xylose binding protein results in a xylose-stimulated xylanase

(2015) Lucas Ferreira Ribeiro et al.   Biotechnology for Biofuels

Molecular Determinants for Protein Stabilization by Insertional Fusion to a Thermophilic Host Protein

(2015) Brennal Pierre et al.   CHEMBIOCHEM

CRISPR-Cas9-based Photoactivatable Transcription System

(2015) Yuta Nihongaki et al.   CHEMISTRY & BIOLOGY

Engineered CRISPR-Cas9 nucleases with altered PAM specificities

(2015) Benjamin P. Kleinstiver et al.   NATURE

Broadening the targeting range of Staphylococcus aureus CRISPR-Cas9 by modifying PAM recognition

(2015) Benjamin P Kleinstiver et al.   NATURE BIOTECHNOLOGY

A split-Cas9 architecture for inducible genome editing and transcription modulation

(2015) Bernd Zetsche et al.   NATURE BIOTECHNOLOGY

Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers

(2015) Isaac B Hilton et al.   NATURE BIOTECHNOLOGY

Photoactivatable CRISPR-Cas9 for optogenetic genome editing

(2015) Yuta Nihongaki et al.   NATURE BIOTECHNOLOGY

Small molecule–triggered Cas9 protein with improved genome-editing specificity

(2015) Kevin M Davis et al.   Nature Chemical Biology

A light-inducible CRISPR-Cas9 system for control of endogenous gene activation

(2015) Lauren R Polstein et al.   Nature Chemical Biology

Therapeutic genome editing: prospects and challenges

(2015) David Benjamin Turitz Cox et al.   NATURE MEDICINE

DNA-binding-domain fusions enhance the targeting range and precision of Cas9

(2015) Mehmet Fatih Bolukbasi et al.   NATURE METHODS

Functional annotation of native enhancers with a Cas9–histone demethylase fusion

(2015) Nicola A Kearns et al.   NATURE METHODS

Development of an intein-mediated split–Cas9 system for gene therapy

(2015) Dong-Jiunn Jeffery Truong et al.   NUCLEIC ACIDS RESEARCH

Multicolor CRISPR labeling of chromosomal loci in human cells

(2015) Hanhui Ma et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Rational design of a split-Cas9 enzyme complex

(2015) Addison V. Wright et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Rationally engineered Cas9 nucleases with improved specificity

(2015) I. M. Slaymaker et al.   SCIENCE

Synthetic protein switches: design principles and applications

(2015) Viktor Stein et al.   TRENDS IN BIOTECHNOLOGY

Off-target Effects in CRISPR/Cas9-mediated Genome Engineering

(2015) Xiao-Hui Zhang et al.   Molecular Therapy-Nucleic Acids

Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA

(2014) Hiroshi Nishimasu et al.   CELL

Development and Applications of CRISPR-Cas9 for Genome Engineering

(2014) Patrick D. Hsu et al.   CELL

Application of TALEs, CRISPR/Cas and sRNAs as trans-acting regulators in prokaryotes

(2014) Matthew F Copeland et al.   CURRENT OPINION IN BIOTECHNOLOGY

Cut Site Selection by the Two Nuclease Domains of the Cas9 RNA-guided Endonuclease

(2014) Hongfan Chen et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structural basis of PAM-dependent target DNA recognition by the Cas9 endonuclease

(2014) Carolin Anders et al.   NATURE

DNA interrogation by the CRISPR RNA-guided endonuclease Cas9

(2014) Samuel H. Sternberg et al.   NATURE

Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing

(2014) Shengdar Q Tsai et al.   NATURE BIOTECHNOLOGY

Fusion of catalytically inactive Cas9 to FokI nuclease improves the specificity of genome modification

(2014) John P Guilinger et al.   NATURE BIOTECHNOLOGY

Direct observation of R-loop formation by single RNA-guided Cas9 and Cascade effector complexes

(2014) Mark D. Szczelkun et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structures of Cas9 Endonucleases Reveal RNA-Mediated Conformational Activation

(2014) M. Jinek et al.   SCIENCE

Adeno-associated virus: fit to serve

(2014) Eric Zinn et al.   Current Opinion in Virology

Visualization of specific DNA sequences in living mouse embryonic stem cells with a programmable fluorescent CRISPR/Cas system

(2014) Tobias Anton et al.   Nucleus

Dynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System

(2013) Baohui Chen et al.   CELL

High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity

(2013) Vikram Pattanayak et al.   NATURE BIOTECHNOLOGY

RNA-guided editing of bacterial genomes using CRISPR-Cas systems

(2013) Wenyan Jiang et al.   NATURE BIOTECHNOLOGY

Cas9 as a versatile tool for engineering biology

(2013) Prashant Mali et al.   NATURE METHODS

Orthogonal Cas9 proteins for RNA-guided gene regulation and editing

(2013) Kevin M Esvelt et al.   NATURE METHODS

Phylogeny of Cas9 determines functional exchangeability of dual-RNA and Cas9 among orthologous type II CRISPR-Cas systems

(2013) Ines Fonfara et al.   NUCLEIC ACIDS RESEARCH

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

Inteins: nature's gift to protein chemists

(2013) Neel H. Shah et al.   Chemical Science

Direct Ubiquitin Independent Recognition and Degradation of a Folded Protein by the Eukaryotic Proteasomes-Origin of Intrinsic Degradation Signals

(2012) Amit Kumar Singh Gautam et al.   PLoS One

Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria

(2012) G. Gasiunas et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Directed Evolution of a Small-Molecule-Triggered Intein with Improved Splicing Properties in Mammalian Cells

(2011) Sun H. Peck et al.   CHEMISTRY & BIOLOGY

Engineering Bifunctional Laccase-Xylanase Chimeras for Improved Catalytic Performance

(2011) Lucas F. Ribeiro et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Short motif sequences determine the targets of the prokaryotic CRISPR defence system

(2009)   MICROBIOLOGY-SGM

Enzyme stabilization by domain insertion into a thermophilic protein

(2009) C.-S. Kim et al.   PROTEIN ENGINEERING DESIGN & SELECTION

The Evolutionary Mechanics of Domain Organization in Proteomes and the Rise of Modularity in the Protein World

(2009) Minglei Wang et al.   STRUCTURE

Structure Prediction of Domain Insertion Proteins from Structures of Individual Domains

(2008) Monica Berrondo et al.   STRUCTURE