Non-viral and viral delivery systems for CRISPR-Cas9 technology in the biomedical field

Cpf1 nucleases demonstrate robust activity to induce DNA modification by exploiting homology directed repair pathways in mammalian cells

(2016) Eszter Tóth et al.   Biology Direct

CRISPR (Clustered Regularly Interspaced Palindromic Repeat)/Cas9 System

(2016) Nikhil V. Munshi   CIRCULATION

Highly multiplexed CRISPR-Cas9-nuclease and Cas9-nickase vectors for inactivation of hepatitis B virus

(2016) Tetsushi Sakuma et al.   GENES TO CELLS

In Vivo Delivery Systems for Therapeutic Genome Editing

(2016) Luyao Wang et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Delivery methods for site-specific nucleases: Achieving the full potential of therapeutic gene editing

(2016) Jia Liu et al.   JOURNAL OF CONTROLLED RELEASE

CRISPR-mediated Genome Editing Restores Dystrophin Expression and Function in mdx Mice

(2016) Li Xu et al.   MOLECULAR THERAPY

Chinese scientists to pioneer first human CRISPR trial

(2016) David Cyranoski   NATURE

Frizzled proteins are colonic epithelial receptors for C. difficile toxin B

(2016) Liang Tao et al.   NATURE

High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects

(2016) Benjamin P. Kleinstiver et al.   NATURE

A dual AAV system enables the Cas9-mediated correction of a metabolic liver disease in newborn mice

(2016) Yang Yang et al.   NATURE BIOTECHNOLOGY

Therapeutic genome editing by combined viral and non-viral delivery of CRISPR system components in vivo

(2016) Hao Yin et al.   NATURE BIOTECHNOLOGY

Efficient genome engineering approaches for the short-lived African turquoise killifish

(2016) Itamar Harel et al.   Nature Protocols

Epigenomic engineering for Down syndrome

(2016) A.F. Mentis   NEUROSCIENCE AND BIOBEHAVIORAL REVIEWS

CRISPR-Cas: biology, mechanisms and relevance

(2016) Frank Hille et al.   PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES

DNA-guided genome editing tool

(2016) Dandan Zhang et al.   Science China-Life Sciences

From the first human gene-editing to the birth of three-parent baby

(2016) Xiaoxue Zhang et al.   Science China-Life Sciences

Advances in therapeutic CRISPR/Cas9 genome editing

(2016) Nataša Savić et al.   Translational Research

Editing of mouse and human immunoglobulin genes by CRISPR-Cas9 system

(2016) Taek-Chin Cheong et al.   Nature Communications

A Retroviral CRISPR-Cas9 System for Cellular Autism-Associated Phenotype Discovery in Developing Neurons

(2016) Michael R. Williams et al.   Scientific Reports

Super-Enhancers at the Nanog Locus Differentially Regulate Neighboring Pluripotency-Associated Genes

(2016) Steven Blinka et al.   Cell Reports

Designing, Packaging, and Delivery of High Titer CRISPR Retro and Lentiviruses via Stereotaxic Injection

(2016) Catherine J. Fricano-Kugler et al.   Jove-Journal of Visualized Experiments

CRISPR-Cas9 directed knock-out of a constitutively expressed gene using lance array nanoinjection

(2016) John W. Sessions et al.   SpringerPlus

Somatic cell reprogramming-free generation of genetically modified pigs

(2016) F. Tanihara et al.   Science Advances

Generation of VDR Knock-Out Mice via Zygote Injection of CRISPR/Cas9 System

(2016) Tao Zhang et al.   PLoS One

Ovarian cancer treatment with a tumor-targeting and gene expression-controllable lipoplex

(2016) Zhi-Yao He et al.   Scientific Reports

CRISPR-Cas9 mediated efficient PD-1 disruption on human primary T cells from cancer patients

(2016) Shu Su et al.   Scientific Reports

Optimization of CRISPR/Cas9 genome editing to modify abiotic stress responses in plants

(2016) Yuriko Osakabe et al.   Scientific Reports

A novel technique based on in vitro oocyte injection to improve CRISPR/Cas9 gene editing in zebrafish

(2016) Shao-Lin Xie et al.   Scientific Reports

CRISPR/Cas9-Mediated Genome Editing of Epigenetic Factors for Cancer Therapy

(2015) Shaohua Yao et al.   HUMAN GENE THERAPY

Adenovirus-Mediated Somatic Genome Editing ofPtenby CRISPR/Cas9 in Mouse Liver in Spite of Cas9-Specific Immune Responses

(2015) Dan Wang et al.   HUMAN GENE THERAPY

Efficiently Editing the Vaccinia Virus Genome by Using the CRISPR-Cas9 System

(2015) Ming Yuan et al.   JOURNAL OF VIROLOGY

In vivo genome editing using Staphylococcus aureus Cas9

(2015) F. Ann Ran et al.   NATURE

Increasing the efficiency of precise genome editing with CRISPR-Cas9 by inhibition of nonhomologous end joining

(2015) Takeshi Maruyama et al.   NATURE BIOTECHNOLOGY

Small molecule–triggered Cas9 protein with improved genome-editing specificity

(2015) Kevin M Davis et al.   Nature Chemical Biology

Therapeutic genome editing: prospects and challenges

(2015) David Benjamin Turitz Cox et al.   NATURE MEDICINE

Development of an intein-mediated split–Cas9 system for gene therapy

(2015) Dong-Jiunn Jeffery Truong et al.   NUCLEIC ACIDS RESEARCH

Generation of knock-in primary human T cells using Cas9 ribonucleoproteins

(2015) Kathrin Schumann et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Rationally engineered Cas9 nucleases with improved specificity

(2015) I. M. Slaymaker et al.   SCIENCE

Postnatal genome editing partially restores dystrophin expression in a mouse model of muscular dystrophy

(2015) C. Long et al.   SCIENCE

In vivo gene editing in dystrophic mouse muscle and muscle stem cells

(2015) M. Tabebordbar et al.   SCIENCE

In vivo genome editing improves muscle function in a mouse model of Duchenne muscular dystrophy

(2015) C. E. Nelson et al.   SCIENCE

Translational Medicine Center of West China Hospital

(2015) Hongxin Deng et al.   Science China-Life Sciences

Suppression of hepatitis B virus DNA accumulation in chronically infected cells using a bacterial CRISPR/Cas RNA-guided DNA endonuclease

(2015) Edward M. Kennedy et al.   VIROLOGY

An Inducible Lentiviral Guide RNA Platform Enables the Identification of Tumor-Essential Genes and Tumor-Promoting Mutations In Vivo

(2015) Brandon J. Aubrey et al.   Cell Reports

A marker-free system for highly efficient construction of vaccinia virus vectors using CRISPR Cas9

(2015) Ming Yuan et al.   Molecular Therapy-Methods & Clinical Development

Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA

(2014) Hiroshi Nishimasu et al.   CELL

Generation of Gene-Modified Cynomolgus Monkey via Cas9/RNA-Mediated Gene Targeting in One-Cell Embryos

(2014) Yuyu Niu et al.   CELL

Efficient Ablation of Genes in Human Hematopoietic Stem and Effector Cells using CRISPR/Cas9

(2014) Pankaj K. Mandal et al.   Cell Stem Cell

Gene disruption by cell-penetrating peptide-mediated delivery of Cas9 protein and guide RNA

(2014) S. Ramakrishna et al.   GENOME RESEARCH

Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins

(2014) S. Kim et al.   GENOME RESEARCH

Targeting Cdk11 in osteosarcoma cells using the CRISPR-cas9 system

(2014) Yong Feng et al.   JOURNAL OF ORTHOPAEDIC RESEARCH

Inactivation of the Human Papillomavirus E6 or E7 Gene in Cervical Carcinoma Cells by Using a Bacterial CRISPR/Cas RNA-Guided Endonuclease

(2014) E. M. Kennedy et al.   JOURNAL OF VIROLOGY

Engineering large viral DNA genomes using the CRISPR-Cas9 system

(2014) Tadahiro Suenaga et al.   MICROBIOLOGY AND IMMUNOLOGY

CRISPR-mediated direct mutation of cancer genes in the mouse liver

(2014) Wen Xue et al.   NATURE

In vivo interrogation of gene function in the mammalian brain using CRISPR-Cas9

(2014) Lukasz Swiech et al.   NATURE BIOTECHNOLOGY

Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo

(2014) John A Zuris et al.   NATURE BIOTECHNOLOGY

Improving CRISPR-Cas nuclease specificity using truncated guide RNAs

(2014) Yanfang Fu et al.   NATURE BIOTECHNOLOGY

Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing

(2014) Shengdar Q Tsai et al.   NATURE BIOTECHNOLOGY

Genome editing with Cas9 in adult mice corrects a disease mutation and phenotype

(2014) Hao Yin et al.   NATURE BIOTECHNOLOGY

CRISPR/Cas9 systems have off-target activity with insertions or deletions between target DNA and guide RNA sequences

(2014) Y. Lin et al.   NUCLEIC ACIDS RESEARCH

Synthesizing AND gate genetic circuits based on CRISPR-Cas9 for identification of bladder cancer cells

(2014) Yuchen Liu et al.   Nature Communications

Adenoviral vector delivery of RNA-guided CRISPR/Cas9 nuclease complexes induces targeted mutagenesis in a diverse array of human cells

(2014) Ignazio Maggio et al.   Scientific Reports

The CRISPR/Cas9 System Facilitates Clearance of the Intrahepatic HBV Templates In Vivo

(2014) Su-Ru Lin et al.   Molecular Therapy-Nucleic Acids

Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases

(2013) S. W. Cho et al.   GENOME RESEARCH

RNA-guided gene activation by CRISPR-Cas9–based transcription factors

(2013) Pablo Perez-Pinera et al.   NATURE METHODS

Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system

(2013) Li-En Jao et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells

(2013) Ophir Shalem et al.   SCIENCE

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering

(2013) Thomas Gaj et al.   TRENDS IN BIOTECHNOLOGY

TALENs: a widely applicable technology for targeted genome editing

(2012) J. Keith Joung et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Safety, Activity, and Immune Correlates of Anti–PD-1 Antibody in Cancer

(2012) Suzanne L. Topalian et al.   NEW ENGLAND JOURNAL OF MEDICINE

Nonviral Direct Conversion of Primary Mouse Embryonic Fibroblasts to Neuronal Cells

(2012) Andrew F Adler et al.   Molecular Therapy-Nucleic Acids

The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA

(2010) Josiane E. Garneau et al.   NATURE

Genome editing with engineered zinc finger nucleases

(2010) Fyodor D. Urnov et al.   NATURE REVIEWS GENETICS