Organoid engineering with microfluidics and biomaterials for liver, lung disease, and cancer modeling

Hydrogel-based colorectal cancer organoid co-culture models

(2021) Xiaobei Luo et al.   Acta Biomaterialia

Probing prodrug metabolism and reciprocal toxicity with an integrated and humanized multi-tissue organ-on-a-chip platform

(2020) Shiny Amala Priya Rajan et al.   Acta Biomaterialia

Cancer statistics, 2020

(2020) Rebecca L. Siegel et al.   CA-A CANCER JOURNAL FOR CLINICIANS

Gastrointestinal tract modeling using organoids engineered with cellular and microbiota niches

(2020) Sungjin Min et al.   EXPERIMENTAL AND MOLECULAR MEDICINE

NAFLD 2020: The State of the Disease

(2020) Thomas G. Cotter et al.   GASTROENTEROLOGY

Predictive biomarkers and mechanisms underlying resistance to PD1/PD-L1 blockade cancer immunotherapy

(2020) Daixi Ren et al.   Molecular Cancer

Fast and efficient generation of knock-in human organoids using homology-independent CRISPR–Cas9 precision genome editing

(2020) Benedetta Artegiani et al.   NATURE CELL BIOLOGY

Characterization of Influenza A Virus Infection in Mouse Pulmonary Stem/Progenitor Cells

(2020) Tai-Ling Chao et al.   Frontiers in Microbiology

Polymer Hydrogels to Guide Organotypic and Organoid Cultures

(2020) Valentina Magno et al.   ADVANCED FUNCTIONAL MATERIALS

Efficacy of primary liver organoid culture from different stages of non-alcoholic steatohepatitis (NASH) mouse model

(2020) Mohamed Elbadawy et al.   BIOMATERIALS

Tumor microenvironment complexity and therapeutic implications at a glance

(2020) Roghayyeh Baghban et al.   Cell Communication and Signaling

Recapitulating idiopathic pulmonary fibrosis related alveolar epithelial dysfunction in a human iPSC‐derived air‐liquid interface model

(2020) Eva Schruf et al.   FASEB JOURNAL

Human-scale lung regeneration based on decellularized matrix scaffolds as a biologic platform

(2020) Keiji Ohata et al.   SURGERY TODAY

Gaining New Biological and Therapeutic Applications into the Liver with 3D In Vitro Liver Models

(2020) Sang Woo Lee et al.   Tissue Engineering and Regenerative Medicine

In Vitro Modeling of the Tumor Microenvironment in Tumor Organoids

(2020) Mahesh Devarasetty et al.   Tissue Engineering and Regenerative Medicine

Decellularized Extracellular Matrix for Bioengineering Physiomimetic 3D in Vitro Tumor Models

(2020) Luís P. Ferreira et al.   TRENDS IN BIOTECHNOLOGY

An organoid biobank for childhood kidney cancers that captures disease and tissue heterogeneity

(2020) Camilla Calandrini et al.   Nature Communications

Recapitulation of SARS-CoV-2 infection and cholangiocyte damage with human liver ductal organoids

(2020) Bing Zhao et al.   Protein & Cell

Human Liver Spheroids as a Model to Study Aetiology and Treatment of Hepatic Fibrosis

(2020) Tracey Hurrell et al.   Cells

Recapitulating Pancreatic Tumor Microenvironment through Synergistic Use of Patient Organoids and Organ‐on‐a‐Chip Vasculature

(2020) Benjamin Fook Lun Lai et al.   ADVANCED FUNCTIONAL MATERIALS

A Chemically Defined Hydrogel for Human Liver Organoid Culture

(2020) Shicheng Ye et al.   ADVANCED FUNCTIONAL MATERIALS

A Human Pluripotent Stem Cell-based Platform to Study SARS-CoV-2 Tropism and Model Virus Infection in Human Cells and Organoids

(2020) Liuliu Yang et al.   Cell Stem Cell

Human organoids: model systems for human biology and medicine

(2020) Jihoon Kim et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Mechano-modulatory synthetic niches for liver organoid derivation

(2020) Giovanni Sorrentino et al.   Nature Communications

High-throughput automated organoid culture via stem-cell aggregation in microcavity arrays

(2020) Nathalie Brandenberg et al.   Nature Biomedical Engineering

Human Lung Adenocarcinoma-Derived Organoid Models for Drug Screening

(2020) Zhichao Li et al.   iScience

Building three-dimensional lung models for studying pharmacokinetics of inhaled drugs

(2020) Andreia Sofia Barros et al.   ADVANCED DRUG DELIVERY REVIEWS

Spheroids as a Type of Three-Dimensional Cell Cultures—Examples of Methods of Preparation and the Most Important Application

(2020) Kamila Białkowska et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Automated microfluidic platform for dynamic and combinatorial drug screening of tumor organoids

(2020) Brooke Schuster et al.   Nature Communications

Progenitor identification and SARS-CoV-2 infection in human distal lung organoids

(2020) Ameen A. Salahudeen et al.   NATURE

Generation of lung organoids from human pluripotent stem cells in vitro

(2019) Alyssa J. Miller et al.   Nature Protocols

Cocultures of human colorectal tumor spheroids with immune cells reveal the therapeutic potential of MICA/B and NKG2A targeting for cancer treatment

(2019) Tristan Courau et al.   Journal for ImmunoTherapy of Cancer

Engineering Microfluidic Organoid-on-a-Chip Platforms

(2019) Fang Yu et al.   Micromachines

TGF-β activation impairs fibroblast ability to support adult lung epithelial progenitor cell organoid formation

(2019) John-Poul Ng-Blichfeldt et al.   AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY

Authentic Modeling of Human Respiratory Virus Infection in Human Pluripotent Stem Cell-Derived Lung Organoids

(2019) M. Porotto et al.   mBio

Establishment of Patient-Derived Organoids and Drug Screening for Biliary Tract Carcinoma

(2019) Yoshimasa Saito et al.   Cell Reports

Application of Cancer Organoid Model for Drug Screening and Personalized Therapy

(2019) Jumpei Kondo et al.   Cells

Mechanically and chemically defined hydrogel matrices for patient-derived colorectal tumor organoid culture

(2019) Shengyong Ng et al.   BIOMATERIALS

Modeling Steatohepatitis in Humans with Pluripotent Stem Cell-Derived Organoids

(2019) Rie Ouchi et al.   Cell Metabolism

Non-contact co-culture with human vascular endothelial cells promotes epithelial-to-mesenchymal transition of cervical cancer SiHa cells by activating the NOTCH1/LOX/SNAIL pathway

(2019) Jinghua Ou et al.   CELLULAR & MOLECULAR BIOLOGY LETTERS

Extracellular vesicle release from intestinal organoids is modulated by Apc mutation and other colorectal cancer progression factors

(2019) Zsuzsanna Szvicsek et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

Disease modelling in human organoids

(2019) Madeline A. Lancaster et al.   Disease Models & Mechanisms

Generation of expandable human pluripotent stem cell-derived hepatocyte-like liver organoids

(2019) Seon Ju Mun et al.   JOURNAL OF HEPATOLOGY

Modeling of Fibrotic Lung Disease Using 3D Organoids Derived from Human Pluripotent Stem Cells

(2019) Alexandros Strikoudis et al.   Cell Reports

Cancer Cell Lines Are Useful Model Systems for Medical Research

(2019) Mirabelli et al.   Cancers

The role of collagen in cancer: from bench to bedside

(2019) Shuaishuai Xu et al.   Journal of Translational Medicine

Modelling human hepato-biliary-pancreatic organogenesis from the foregut–midgut boundary

(2019) Hiroyuki Koike et al.   NATURE

Engineered materials for organoid systems

(2019) Michael J. Kratochvil et al.   Nature Reviews Materials

A view on drug resistance in cancer

(2019) Neil Vasan et al.   NATURE

The great escape: tumour cell plasticity in resistance to targeted therapy

(2019) Soufiane Boumahdi et al.   NATURE REVIEWS DRUG DISCOVERY

Patient-derived lung cancer organoids as in vitro cancer models for therapeutic screening

(2019) Minsuh Kim et al.   Nature Communications

Engineered Liver-on-a-Chip Platform to Mimic Liver Functions and Its Biomedical Applications: A Review

(2019) Deng et al.   Micromachines

Robust, Long-Term Culture of Endoderm-Derived Hepatic Organoids for Disease Modeling

(2019) Soheil Akbari et al.   Stem Cell Reports

A high-throughput drug combination screen of targeted small molecule inhibitors in cancer cell lines

(2019) Åsmund Flobak et al.   Scientific Data

Immune cells within the tumor microenvironment: Biological functions and roles in cancer immunotherapy

(2019) Xu Lei et al.   CANCER LETTERS

Progressive Pulmonary Fibrosis Is Caused by Elevated Mechanical Tension on Alveolar Stem Cells

(2019) Huijuan Wu et al.   CELL

Pancreatic cancer organoids recapitulate disease and allow personalized drug screening

(2019) Else Driehuis et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Extracellular matrix hydrogel derived from decellularized tissues enables endodermal organoid culture

(2019) Giovanni Giuseppe Giobbe et al.   Nature Communications

Generation of matched patient-derived xenograft in vitro-in vivo models using 3D macroporous hydrogels for the study of liver cancer

(2018) Eliza Li Shan Fong et al.   BIOMATERIALS

Development of primary human pancreatic cancer organoids, matched stromal and immune cells and 3D tumor microenvironment models

(2018) Susan Tsai et al.   BMC CANCER

Patient-derived organoid models help define personalized management of gastrointestinal cancer

(2018) M. R. Aberle et al.   BRITISH JOURNAL OF SURGERY

A Living Biobank of Breast Cancer Organoids Captures Disease Heterogeneity

(2018) Norman Sachs et al.   CELL

Single Particle Imaging of Polarized Hepatoma Organoids upon Hepatitis C Virus Infection Reveals an Ordered and Sequential Entry Process

(2018) Yasmine Baktash et al.   Cell Host & Microbe

Human Pancreatic Tumor Organoids Reveal Loss of Stem Cell Niche Factor Dependence during Disease Progression

(2018) Takashi Seino et al.   Cell Stem Cell

Generation of Hepatic Stellate Cells from Human Pluripotent Stem Cells Enables In Vitro Modeling of Liver Fibrosis

(2018) Mar Coll et al.   Cell Stem Cell

Design and fabrication of a liver-on-a-chip platform for convenient, highly efficient, and safe in situ perfusion culture of 3D hepatic spheroids

(2018) Li-Dong Ma et al.   LAB ON A CHIP

Extracellular matrix in lung development, homeostasis and disease

(2018) Yong Zhou et al.   MATRIX BIOLOGY

Differentiated human airway organoids to assess infectivity of emerging influenza virus

(2018) Jie Zhou et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Patient-derived organoids model treatment response of metastatic gastrointestinal cancers

(2018) Georgios Vlachogiannis et al.   SCIENCE

Patient-derived tumor organoids for prediction of cancer treatment response

(2018) Peter W. Nagle et al.   SEMINARS IN CANCER BIOLOGY

Reconstituting Mouse Lungs with Conditionally Reprogrammed Human Bronchial Epithelial Cells

(2018) Ryan LaRanger et al.   TISSUE ENGINEERING PART A

A Novel Three-Dimensional Human Tissue-Engineered Lung Model to Study Influenza A Infection

(2018) Rudra Bhowmick et al.   TISSUE ENGINEERING PART A

Human lung epithelial cell cultures for analysis of inhaled toxicants: Lessons learned and future directions

(2018) Pieter S. Hiemstra et al.   TOXICOLOGY IN VITRO

3D microfluidic liver cultures as a physiological preclinical tool for hepatitis B virus infection

(2018) A. M. Ortega-Prieto et al.   Nature Communications

A microfluidics platform for combinatorial drug screening on cancer biopsies

(2018) Federica Eduati et al.   Nature Communications

Organoid profiling identifies common responders to chemotherapy in pancreatic cancer

(2018) Hervé Tiriac et al.   Cancer Discovery

In vitro patient-derived 3D mesothelioma tumor organoids facilitate patient-centric therapeutic screening

(2018) Andrea R. Mazzocchi et al.   Scientific Reports

Enterovirus 71 infection of human airway organoids reveals VP1-145 as a viral infectivity determinant

(2018) Sabine M. G. van der Sanden et al.   Emerging Microbes & Infections

Three-dimensional brain-like microenvironments facilitate the direct reprogramming of fibroblasts into therapeutic neurons

(2018) Yoonhee Jin et al.   Nature Biomedical Engineering

Growth of Epithelial Organoids in a Defined Hydrogel

(2018) Nicolas Broguiere et al.   ADVANCED MATERIALS

Divergent Routes toward Wnt and R-spondin Niche Independency during Human Gastric Carcinogenesis

(2018) Kosaku Nanki et al.   CELL

Generation of Tumor-Reactive T Cells by Co-culture of Peripheral Blood Lymphocytes and Tumor Organoids

(2018) Krijn K. Dijkstra et al.   CELL

Burden of Liver Diseases in the World

(2018) Sumeet K. Asrani et al.   JOURNAL OF HEPATOLOGY

In Situ Cross-Linkable Hydrogels as a Dynamic Matrix for Tissue Regenerative Medicine

(2018) Kyung Min Park et al.   Tissue Engineering and Regenerative Medicine

Human hepatic 3D spheroids as a model for steatosis and insulin resistance

(2018) Mikael Kozyra et al.   Scientific Reports

Organoid Models of Human Liver Cancers Derived from Tumor Needle Biopsies

(2018) Sandro Nuciforo et al.   Cell Reports

A 3D human lung-on-a-chip model for nanotoxicity testing

(2018) Min Zhang et al.   Toxicology Research

Recapitulation of hepatitis B virus–host interactions in liver organoids from human induced pluripotent stem cells

(2018) Yun-Zhong Nie et al.   EBioMedicine

Idiopathic Pulmonary Fibrosis (IPF): An Overview

(2018) Shaney Barratt et al.   Journal of Clinical Medicine

Cystic Fibrosis: Emerging Understanding and Therapies

(2018) Michael M. Rey et al.   Annual Review of Medicine

Organoid Modeling of the Tumor Immune Microenvironment

(2018) James T. Neal et al.   CELL

A Comprehensive Human Gastric Cancer Organoid Biobank Captures Tumor Subtype Heterogeneity and Enables Therapeutic Screening

(2018) Helen H.N. Yan et al.   Cell Stem Cell

Small Animal Models of Respiratory Viral Infection Related to Asthma

(2018) Mingyuan Han et al.   Viruses-Basel

Distinct populations of inflammatory fibroblasts and myofibroblasts in pancreatic cancer

(2017) Daniel Öhlund et al.   JOURNAL OF EXPERIMENTAL MEDICINE

Guided self-organization and cortical plate formation in human brain organoids

(2017) Madeline A Lancaster et al.   NATURE BIOTECHNOLOGY

Synthetic hydrogels for human intestinal organoid generation and colonic wound repair

(2017) Ricardo Cruz-Acuña et al.   NATURE CELL BIOLOGY

Fused cerebral organoids model interactions between brain regions

(2017) Joshua A Bagley et al.   NATURE METHODS

Animal models of respiratory syncytial virus infection

(2017) Geraldine Taylor   VACCINE

Biomaterials-Based Approaches to Tumor Spheroid and Organoid Modeling

(2017) Pradip Shahi Thakuri et al.   Advanced Healthcare Materials

Human Pluripotent Stem Cell-Derived Cardiac Tissue-like Constructs for Repairing the Infarcted Myocardium

(2017) Junjun Li et al.   Stem Cell Reports

Long-Term Adult Feline Liver Organoid Cultures for Disease Modeling of Hepatic Steatosis

(2017) Hedwig S. Kruitwagen et al.   Stem Cell Reports

Metabolic zonation of the liver: The oxygen gradient revisited

(2017) Thomas Kietzmann   Redox Biology

Pro-fibrotic compounds induce stellate cell activation, ECM-remodelling and Nrf2 activation in a human 3D-multicellular model of liver fibrosis

(2017) Vincenzo Prestigiacomo et al.   PLoS One

A perfusion incubator liver chip for 3D cell culture with application on chronic hepatotoxicity testing

(2017) Fang Yu et al.   Scientific Reports

Organoids as a model system for studying human lung development and disease

(2016) Rohan R. Nadkarni et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Novel human hepatic organoid model enables testing of drug-induced liver fibrosis in vitro

(2016) Sofia B. Leite et al.   BIOMATERIALS

Comparative biology of decellularized lung matrix: Implications of species mismatch in regenerative medicine

(2016) Jenna L. Balestrini et al.   BIOMATERIALS

Modeling Development and Disease with Organoids

(2016) Hans Clevers   CELL

Using the mouse to model human disease: increasing validity and reproducibility

(2016) Monica J. Justice et al.   Disease Models & Mechanisms

Global epidemiology of nonalcoholic fatty liver disease-Meta-analytic assessment of prevalence, incidence, and outcomes

(2016) Zobair M. Younossi et al.   HEPATOLOGY

Pharmacogenetics: The right drug for you

(2016) Liam Drew   NATURE

Designer matrices for intestinal stem cell and organoid culture

(2016) Nikolce Gjorevski et al.   NATURE

Collagen-rich stroma in aggressive colon tumors induces mesenchymal gene expression and tumor cell invasion

(2016) T T Vellinga et al.   ONCOGENE

Development of a Three-Dimensional Bioengineering Technology to Generate Lung Tissue for Personalized Disease Modeling

(2016) Dan C. Wilkinson et al.   Stem Cells Translational Medicine

Advances in Engineered Liver Models for Investigating Drug-Induced Liver Injury

(2016) Christine Lin et al.   Biomed Research International

A Well-Controlled Experimental System to Study Interactions of Cytotoxic T Lymphocytes with Tumor Cells

(2016) Natalie J. Neubert et al.   Frontiers in Immunology

The alterations in the extracellular matrix composition guide the repair of damaged liver tissue

(2016) Mariliis Klaas et al.   Scientific Reports

Experimental models of liver fibrosis

(2015) Sara Crespo Yanguas et al.   ARCHIVES OF TOXICOLOGY

A microfluidically perfused three dimensional human liver model

(2015) Knut Rennert et al.   BIOMATERIALS

Long-term maintenance of a microfluidic 3D human liver sinusoid

(2015) Ljupcho Prodanov et al.   BIOTECHNOLOGY AND BIOENGINEERING

Long-Term Culture of Genome-Stable Bipotent Stem Cells from Adult Human Liver

(2015) Meritxell Huch et al.   CELL

Prospective Derivation of a Living Organoid Biobank of Colorectal Cancer Patients

(2015) Marc van de Wetering et al.   CELL

3D tissue-like assemblies: A novel approach to investigate virus–cell interactions

(2015) Thomas J. Goodwin et al.   METHODS

Small airway-on-a-chip enables analysis of human lung inflammation and drug responses in vitro

(2015) Kambez H Benam et al.   NATURE METHODS

Alternatives to animal testing: A review

(2015) Sonali K. Doke et al.   SAUDI PHARMACEUTICAL JOURNAL

Physical supports from liver cancer cells are essential for differentiation and remodeling of endothelial cells in a HepG2-HUVEC co-culture model

(2015) Geraldine Giap Ying Chiew et al.   Scientific Reports

In vitro generation of human pluripotent stem cell derived lung organoids

(2015) Briana R Dye et al.   eLife

Direct Interaction between Carcinoma Cells and Cancer Associated Fibroblasts for the Regulation of Cancer Invasion

(2015) Hideki Yamaguchi et al.   Cancers

Organoid Cultures Derived from Patients with Advanced Prostate Cancer

(2014) Dong Gao et al.   CELL

Hepatic organoids for microfluidic drug screening

(2014) Sam H. Au et al.   LAB ON A CHIP

Microfluidic organs-on-chips

(2014) Sangeeta N Bhatia et al.   NATURE BIOTECHNOLOGY

Development of an Innovative 3D Cell Culture System to Study Tumour - Stroma Interactions in Non-Small Cell Lung Cancer Cells

(2014) Arno Amann et al.   PLoS One

Liver Extracellular Matrix Providing Dual Functions of Two-Dimensional Substrate Coating and Three-Dimensional Injectable Hydrogel Platform for Liver Tissue Engineering

(2013) Jung Seung Lee et al.   BIOMACROMOLECULES

Differential Cell Line Susceptibility to the Emerging Novel Human Betacoronavirus 2c EMC/2012: Implications for Disease Pathogenesis and Clinical Manifestation

(2013) Jasper Fuk-Woo Chan, Kwok-Hung Chan, Garnet Kwan-Yue Choi, Kelvin Kai-Wang To, Herman Tse, Jian-Piao Cai et al.   JOURNAL OF INFECTIOUS DISEASES

Fate tracing reveals hepatic stellate cells as dominant contributors to liver fibrosis independent of its aetiology

(2013) Ingmar Mederacke et al.   Nature Communications

Cystic fibrosis: a mucosal immunodeficiency syndrome

(2012) Taylor Sitarik Cohen et al.   NATURE MEDICINE

Mechanisms of fibrosis: therapeutic translation for fibrotic disease

(2012) Thomas A Wynn et al.   NATURE MEDICINE

The pathogenesis of COPD and IPF: Distinct horns of the same devil?

(2012) Marco Chilosi et al.   RESPIRATORY RESEARCH

A Human Disease Model of Drug Toxicity–Induced Pulmonary Edema in a Lung-on-a-Chip Microdevice

(2012) Dongeun Huh et al.   Science Translational Medicine

Concise Review: The Relevance of Human Stem Cell-Derived Organoid Models for Epithelial Translational Medicine

(2012) Robert E. Hynds et al.   STEM CELLS

Tissue engineered scaffold utilizing the reinforced technique

(2010) Young-Kwon Seo et al.   BIOTECHNOLOGY AND BIOPROCESS ENGINEERING

Reconstituting Organ-Level Lung Functions on a Chip

(2010) D. Huh et al.   SCIENCE

Tissue-specific extracellular matrix coatings for the promotion of cell proliferation and maintenance of cell phenotype

(2009) Yuanyuan Zhang et al.   BIOMATERIALS

Liver Anatomy: Microcirculation of the Liver

(2008) Zuxing Kan et al.   SEMINARS IN INTERVENTIONAL RADIOLOGY