Biomaterials-Based Approaches to Tumor Spheroid and Organoid Modeling

The Use of Endometrial Cancer Patient–Derived Organoid Culture for Drug Sensitivity Testing Is Feasible

(2017) Eugenia Girda et al.   INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER

Synthetic hydrogels mimicking basement membrane matrices to promote cell-matrix interactions

(2017) Ricardo Cruz-Acuña et al.   MATRIX BIOLOGY

Cell diversity and network dynamics in photosensitive human brain organoids

(2017) Giorgia Quadrato et al.   NATURE

Transplantation of engineered organoids enables rapid generation of metastatic mouse models of colorectal cancer

(2017) Kevin P O'Rourke et al.   NATURE BIOTECHNOLOGY

A three-dimensional model of human lung development and disease from pluripotent stem cells

(2017) Ya-Wen Chen et al.   NATURE CELL BIOLOGY

EMT, CSCs, and drug resistance: the mechanistic link and clinical implications

(2017) Tsukasa Shibue et al.   Nature Reviews Clinical Oncology

Disease Modeling in Stem Cell-Derived 3D Organoid Systems

(2017) Devanjali Dutta et al.   TRENDS IN MOLECULAR MEDICINE

Personalized In Vitro and In Vivo Cancer Models to Guide Precision Medicine

(2017) Chantal Pauli et al.   Cancer Discovery

Chitosan promotes cancer progression and stem cell properties in association with Wnt signaling in colon and hepatocellular carcinoma cells

(2017) Po-Hsiang Chang et al.   Scientific Reports

Conformal Nanoencapsulation of Allogeneic T Cells Mitigates Graft-versus-Host Disease and Retains Graft-versus-Leukemia Activity

(2016) Shuting Zhao et al.   ACS Nano

Tailoring mechanical properties of decellularized extracellular matrix bioink by vitamin B2-induced photo-crosslinking

(2016) Jinah Jang et al.   Acta Biomaterialia

Biomimetic Hydrogels Incorporating Polymeric Cell-Adhesive Peptide To Promote the 3D Assembly of Tumoroids

(2016) Ying Hao et al.   BIOMACROMOLECULES

A 3D in vitro model of patient-derived prostate cancer xenograft for controlled interrogation of in vivo tumor-stromal interactions

(2016) Eliza L.S. Fong et al.   BIOMATERIALS

Combined cancer therapy with hyaluronan-decorated fullerene-silica multifunctional nanoparticles to target cancer stem-like cells

(2016) Hai Wang et al.   BIOMATERIALS

Enzymatically prepared redox-responsive hydrogels as potent matrices for hepatocellular carcinoma cell spheroid formation

(2016) Kousuke Moriyama et al.   Biotechnology Journal

Tumor slice culture system to assess drug response of primary breast cancer

(2016) Kishan A. T. Naipal et al.   BMC CANCER

Gene and miRNA expression signature of Lewis lung carcinoma LLC1 cells in extracellular matrix enriched microenvironment

(2016) Vaidotas Stankevicius et al.   BMC CANCER

Liquid-based three-dimensional tumor models for cancer research and drug discovery

(2016) Stephanie L Ham et al.   EXPERIMENTAL BIOLOGY AND MEDICINE

3D printing in pharmaceutics: A new tool for designing customized drug delivery systems

(2016) Jonathan Goole et al.   INTERNATIONAL JOURNAL OF PHARMACEUTICS

PEG-fibrinogen hydrogels for three-dimensional breast cancer cell culture

(2016) Shantanu Pradhan et al.   JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A

Formation of three-dimensional hepatic tissue by the bottom-up method using spheroids

(2016) Tatsuya Okudaira et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Multiparametric Analysis of Oncology Drug Screening with Aqueous Two-Phase Tumor Spheroids

(2016) Pradip Shahi Thakuri et al.   MOLECULAR PHARMACEUTICS

Breast Cancer Stem Cell Culture and Enrichment Using Poly(ε-Caprolactone) Scaffolds

(2016) Sònia Palomeras et al.   MOLECULES

Screening out irrelevant cell-based models of disease

(2016) Peter Horvath et al.   NATURE REVIEWS DRUG DISCOVERY

Zika virus impairs growth in human neurospheres and brain organoids

(2016) P. P. Garcez et al.   SCIENCE

Polymeric Biomaterials forIn VitroCancer Tissue Engineering and Drug Testing Applications

(2016) Shantanu Pradhan et al.   TISSUE ENGINEERING PART B-REVIEWS

Genetically engineering self-organization of human pluripotent stem cells into a liver bud-like tissue using Gata6

(2016) Patrick Guye et al.   Nature Communications

Bioengineering of injectable encapsulated aggregates of pluripotent stem cells for therapy of myocardial infarction

(2016) Shuting Zhao et al.   Nature Communications

Wnt and Neuregulin1/ErbB signalling extends 3D culture of hormone responsive mammary organoids

(2016) Thierry Jardé et al.   Nature Communications

Three-dimensional Organotypic Culture Models of Human Hepatocellular Carcinoma

(2016) Atsushi Takai et al.   Scientific Reports

Engineered Breast Cancer Cell Spheroids Reproduce Biologic Properties of Solid Tumors

(2016) Stephanie L. Ham et al.   Advanced Healthcare Materials

Human Skin 3D Bioprinting Using Scaffold-Free Approach

(2016) Léa J. Pourchet et al.   Advanced Healthcare Materials

Culture on 3D Chitosan-Hyaluronic Acid Scaffolds Enhances Stem Cell Marker Expression and Drug Resistance in Human Glioblastoma Cancer Stem Cells

(2016) Kui Wang et al.   Advanced Healthcare Materials

Enzymatically prepared redox-responsive hydrogels as potent matrices for hepatocellular carcinoma cell spheroid formation

(2016) Kousuke Moriyama et al.   Biotechnology Journal

Establishment of a Novel Model for Anticancer Drug Resistance in Three-Dimensional Primary Culture of Tumor Microenvironment

(2016) Tatsuya Usui et al.   Stem Cells International

Engineering a 3D microfluidic culture platform for tumor-treating field application

(2016) Andrea Pavesi et al.   Scientific Reports

Interfacial Tension Effect on Cell Partition in Aqueous Two-Phase Systems

(2015) Ehsan Atefi et al.   ACS Applied Materials & Interfaces

Target Specific Delivery of Anticancer Drug in Silk Fibroin Based 3D Distribution Model of Bone–Breast Cancer Cells

(2015) Bano Subia et al.   ACS Applied Materials & Interfaces

Chitosan-Decorated Doxorubicin-Encapsulated Nanoparticle Targets and Eliminates Tumor Reinitiating Cancer Stem-like Cells

(2015) Wei Rao et al.   ACS Nano

A Near-Infrared Laser-Activated “Nanobomb” for Breaking the Barriers to MicroRNA Delivery

(2015) Hai Wang et al.   ADVANCED MATERIALS

Hyaluronic acid-decorated dual responsive nanoparticles of Pluronic F127, PLGA, and chitosan for targeted co-delivery of doxorubicin and irinotecan to eliminate cancer stem-like cells

(2015) Hai Wang et al.   BIOMATERIALS

Organoid Models of Human and Mouse Ductal Pancreatic Cancer

(2015) Sylvia F. Boj et al.   CELL

From drug response profiling to target addiction scoring in cancer cell models

(2015) Bhagwan Yadav et al.   Disease Models & Mechanisms

Phytochemicals potently inhibit migration of metastatic breast cancer cells

(2015) Stephanie Lemmo Ham et al.   Integrative Biology

Circulating Tumor Cell Biomarker Panel As an Individual-Level Surrogate for Survival in Metastatic Castration-Resistant Prostate Cancer

(2015) Howard I. Scher et al.   JOURNAL OF CLINICAL ONCOLOGY

Analysis of ERBB Ligand-Induced Resistance Mechanism to Crizotinib by Primary Culture of Lung Adenocarcinoma with EML4-ALK Fusion Gene

(2015) Madoka Kimura et al.   Journal of Thoracic Oncology

Tumor-Derived Cell Lines as Molecular Models of Cancer Pharmacogenomics

(2015) A. Goodspeed et al.   MOLECULAR CANCER RESEARCH

Bioengineering Thymus Organoids to Restore Thymic Function and Induce Donor-Specific Immune Tolerance to Allografts

(2015) Yong Fan et al.   MOLECULAR THERAPY

A three-dimensional engineered tumour for spatial snapshot analysis of cell metabolism and phenotype in hypoxic gradients

(2015) Darren Rodenhizer et al.   NATURE MATERIALS

Ductal pancreatic cancer modeling and drug screening using human pluripotent stem cell– and patient-derived tumor organoids

(2015) Ling Huang et al.   NATURE MEDICINE

Modeling colorectal cancer using CRISPR-Cas9–mediated engineering of human intestinal organoids

(2015) Mami Matano et al.   NATURE MEDICINE

Efficient genetic engineering of human intestinal organoids using electroporation

(2015) Masayuki Fujii et al.   Nature Protocols

Swimming muscles power suction feeding in largemouth bass

(2015) Ariel L. Camp et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Multilayered, Hyaluronic Acid-Based Hydrogel Formulations Suitable for Automated 3D High Throughput Drug Screening of Cancer-Stromal Cell Cocultures

(2015) Brian J. Engel et al.   Advanced Healthcare Materials

Cell Therapy Using Human Induced Pluripotent Stem Cell-Derived Renal Progenitors Ameliorates Acute Kidney Injury in Mice

(2015) Takafumi Toyohara et al.   Stem Cells Translational Medicine

Protein-engineered scaffolds for in vitro 3D culture of primary adult intestinal organoids

(2015) Rebecca L. DiMarco et al.   Biomaterials Science

Targeting self-renewal pathways in cancer stem cells: clinical implications for cancer therapy

(2015) A Borah et al.   Oncogenesis

Spherical Cancer Models in Tumor Biology

(2015) Louis-Bastien Weiswald et al.   NEOPLASIA

Galactosylated reversible hydrogels as scaffold for HepG2 spheroid generation

(2014) Yuhan Wu et al.   Acta Biomaterialia

The need for complex 3D culture models to unravel novel pathways and identify accurate biomarkers in breast cancer

(2014) Britta Weigelt et al.   ADVANCED DRUG DELIVERY REVIEWS

High Throughput, Polymeric Aqueous Two-Phase Printing of Tumor Spheroids

(2014) Ehsan Atefi et al.   ADVANCED FUNCTIONAL MATERIALS

Proliferation and enrichment of CD133+ glioblastoma cancer stem cells on 3D chitosan-alginate scaffolds

(2014) Forrest M. Kievit et al.   BIOMATERIALS

Enhanced enrichment of prostate cancer stem-like cells with miniaturized 3D culture in liquid core-hydrogel shell microcapsules

(2014) Wei Rao et al.   BIOMATERIALS

Acquisition of epithelial–mesenchymal transition and cancer stem-like phenotypes within chitosan-hyaluronan membrane-derived 3D tumor spheroids

(2014) Yen-Jang Huang et al.   BIOMATERIALS

Three-dimensional in vitro tumor models for cancer research and drug evaluation

(2014) Xian Xu et al.   BIOTECHNOLOGY ADVANCES

Paracrine interactions between LNCaP prostate cancer cells and bioengineered bone in 3D in vitro culture reflect molecular changes during bone metastasis

(2014) Shirly Sieh et al.   BONE

Organoid Cultures Derived from Patients with Advanced Prostate Cancer

(2014) Dong Gao et al.   CELL

Optimization of Aqueous Biphasic Tumor Spheroid Microtechnology for Anti-cancer Drug Testing in 3D Culture

(2014) Stephanie Lemmo et al.   Cellular and Molecular Bioengineering

The controllable preparation of porous PLGA microspheres by the oil/water emulsion method and its application in 3D culture of ovarian cancer cells

(2014) Tianzhu Zhang et al.   COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS

How to make an intestine

(2014) J. M. Wells et al.   DEVELOPMENT

The extracellular matrix modulates the hallmarks of cancer

(2014) M. W. Pickup et al.   EMBO REPORTS

3D high-content screening for the identification of compounds that target cells in dormant tumor spheroid regions

(2014) Carsten Wenzel et al.   EXPERIMENTAL CELL RESEARCH

Coaxial electrospray of liquid core–hydrogel shell microcapsules for encapsulation and miniaturized 3D culture of pluripotent stem cells

(2014) Shuting Zhao et al.   Integrative Biology

Enrichment of breast cancer stem-like cells by growth on electrospun polycaprolactone-chitosan nanofiber scaffolds

(2014) Jennifer Sims-Mourtada et al.   International Journal of Nanomedicine

Hydrogel-Based 3D Model of Patient-Derived Prostate Xenograft Tumors Suitable for Drug Screening

(2014) Eliza L. S. Fong et al.   MOLECULAR PHARMACEUTICS

A 3D Biomimetic Model of Tissue Stiffness Interface for Cancer Drug Testing

(2014) Chee Ren Ivan Lam et al.   MOLECULAR PHARMACEUTICS

Oncogenic transformation of diverse gastrointestinal tissues in primary organoid culture

(2014) Xingnan Li et al.   NATURE MEDICINE

Prostate cancer cell-stromal cell crosstalk via FGFR1 mediates antitumor activity of dovitinib in bone metastases

(2014) X. Wan et al.   Science Translational Medicine

Macroscopic Stiffness of Breast Tumors Predicts Metastasis

(2014) Joseph Fenner et al.   Scientific Reports

A novel tissue engineered three-dimensional in vitro colorectal cancer model

(2013) Agata Nyga et al.   Acta Biomaterialia

A multicellular 3D heterospheroid model of liver tumor and stromal cells in collagen gel for anti-cancer drug testing

(2013) Derek Yip et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

The independent roles of mechanical, structural and adhesion characteristics of 3D hydrogels on the regulation of cancer invasion and dissemination

(2013) Jennifer N. Beck et al.   BIOMATERIALS

A microfluidic device for uniform-sized cell spheroids formation, culture, harvesting and flow cytometry analysis

(2013) Bishnubrata Patra et al.   Biomicrofluidics

Aqueous biphasic cancer cell migration assay enables robust, high-throughput screening of anti-cancer compounds

(2013) Stephanie Lemmo et al.   Biotechnology Journal

Chitosan-based biomaterials for tissue engineering

(2013) Florence Croisier et al.   EUROPEAN POLYMER JOURNAL

Cell to extracellular matrix interactions and their reciprocal nature in cancer

(2013) Marianne van Dijk et al.   EXPERIMENTAL CELL RESEARCH

Engineering of three-dimensional microenvironments to promote contractile behavior in primary intestinal organoids

(2013) Rebecca L. DiMarco et al.   Integrative Biology

One-step microfluidic generation of pre-hatching embryo-like core–shell microcapsules for miniaturized 3D culture of pluripotent stem cells

(2013) Pranay Agarwal et al.   LAB ON A CHIP

Bone-stromal cells up-regulate tumourigenic markers in a tumour-stromal 3D model of prostate cancer

(2013) Louisa CE Windus et al.   Molecular Cancer

Vascularized and functional human liver from an iPSC-derived organ bud transplant

(2013) Takanori Takebe et al.   NATURE

Three-dimensional cell culturing by magnetic levitation

(2013) William L Haisler et al.   Nature Protocols

Impact of the 3D Microenvironment on Phenotype, Gene Expression, and EGFR Inhibition of Colorectal Cancer Cell Lines

(2013) Anna C. Luca et al.   PLoS One

A 3D Fibrous Scaffold Inducing Tumoroids: A Platform for Anticancer Drug Development

(2013) Yvonne K. Girard et al.   PLoS One

Modeling Ewing sarcoma tumors in vitro with 3D scaffolds

(2013) E. L. S. Fong et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Rapid Prototyping of Concave Microwells for the Formation of 3D Multicellular Cancer Aggregates for Drug Screening

(2013) Ting-Yuan Tu et al.   Advanced Healthcare Materials

A Non-Mulberry Silk Fibroin Protein Based 3D In Vitro Tumor Model for Evaluation of Anticancer Drug Activity

(2012) Sarmistha Talukdar et al.   ADVANCED FUNCTIONAL MATERIALS

Recreating the tumor microenvironment in a bilayer, hyaluronic acid hydrogel construct for the growth of prostate cancer spheroids

(2012) Xian Xu et al.   BIOMATERIALS

Advances in establishment and analysis of three-dimensional tumor spheroid-based functional assays for target validation and drug evaluation

(2012) Maria Vinci et al.   BMC BIOLOGY

Importance of Intracellular pH in Determining the Uptake and Efficacy of the Weakly Basic Chemotherapeutic Drug, Doxorubicin

(2012) Pawel Swietach et al.   PLoS One

Hyaluronic acid-based hydrogels: from a natural polysaccharide to complex networks

(2012) Xian Xu et al.   Soft Matter

Rehydration of Polymeric, Aqueous, Biphasic System Facilitates High Throughput Cell Exclusion Patterning for Cell Migration Studies

(2011) Hossein Tavana et al.   ADVANCED FUNCTIONAL MATERIALS

Modeling Colon Adenocarcinomas in Vitro

(2011) Helmut Dolznig et al.   AMERICAN JOURNAL OF PATHOLOGY

Thermoreversible Hydrogel for In Situ Generation and Release of HepG2 Spheroids

(2011) Dongdong Wang et al.   BIOMACROMOLECULES

Three-dimensional porous silk tumor constructs in the approximation of in vivo osteosarcoma physiology

(2011) Pamela H.S. Tan et al.   BIOMATERIALS

3D in vitro bioengineered tumors based on collagen I hydrogels

(2011) Christopher S. Szot et al.   BIOMATERIALS

Automated microinjection of cell-polymer suspensions in 3D ECM scaffolds for high-throughput quantitative cancer invasion screens

(2011) Hoa H. Truong et al.   BIOMATERIALS

The enhancement of cancer stem cell properties of MCF-7 cells in 3D collagen scaffolds for modeling of cancer and anti-cancer drugs

(2011) Lei Chen et al.   BIOMATERIALS

Microprinted feeder cells guide embryonic stem cell fate

(2011) Hossein Tavana et al.   BIOTECHNOLOGY AND BIOENGINEERING

Long-term Expansion of Epithelial Organoids From Human Colon, Adenoma, Adenocarcinoma, and Barrett's Epithelium

(2011) Toshiro Sato et al.   GASTROENTEROLOGY

High drug attrition rates—where are we going wrong?

(2011) Lisa Hutchinson et al.   Nature Reviews Clinical Oncology

The productivity crisis in pharmaceutical R&D

(2011) Fabio Pammolli et al.   NATURE REVIEWS DRUG DISCOVERY

Efficacy of and resistance to anti-IGF-1R therapies in Ewing's sarcoma is dependent on insulin receptor signaling

(2011) C Garofalo et al.   ONCOGENE

Additive manufacturing of tissues and organs

(2011) Ferry P.W. Melchels et al.   PROGRESS IN POLYMER SCIENCE

Blockade of transforming growth factor-beta (TGFβ) signaling inhibits osteoblastic tumorigenesis by a novel human prostate cancer cell line

(2011) Sweta Mishra et al.   PROSTATE

Polymeric Aqueous Biphasic Systems for Non-Contact Cell Printing on Cells: Engineering Heterocellular Embryonic Stem Cell Niches

(2010) Hossein Tavana et al.   ADVANCED MATERIALS

Chitosan–alginate 3D scaffolds as a mimic of the glioma tumor microenvironment

(2010) Forrest M. Kievit et al.   BIOMATERIALS

Bioengineered 3D platform to explore cell–ECM interactions and drug resistance of epithelial ovarian cancer cells

(2010) Daniela Loessner et al.   BIOMATERIALS

Engineered silk fibroin protein 3D matrices for in vitro tumor model

(2010) Sarmistha Talukdar et al.   BIOMATERIALS

The microenvironment determines the breast cancer cells' phenotype: organization of MCF7 cells in 3D cultures

(2010) Silva Krause et al.   BMC CANCER

Principles of early drug discovery

(2010) JP Hughes et al.   BRITISH JOURNAL OF PHARMACOLOGY

Breast Cancer Cells in Three-dimensional Culture Display an Enhanced Radioresponse after Coordinate Targeting of Integrin  5 1 and Fibronectin

(2010) J.-M. Nam et al.   CANCER RESEARCH

Incorporation of multicellular spheroids into 3-D polymeric scaffolds provides an improved tumor model for screening anticancer drugs

(2010) Won Jin Ho et al.   CANCER SCIENCE

Lgr5+ve Stem Cells Drive Self-Renewal in the Stomach and Build Long-Lived Gastric Units In Vitro

(2010) Nick Barker et al.   Cell Stem Cell

Biomaterials offer cancer research the third dimension

(2010) Dietmar W. Hutmacher   NATURE MATERIALS

Chitosan-Alginate Scaffold Culture System for Hepatocellular Carcinoma Increases Malignancy and Drug Resistance

(2010) Matthew Leung et al.   PHARMACEUTICAL RESEARCH

A Comprehensive Panel of Three-Dimensional Models for Studies of Prostate Cancer Growth, Invasion and Drug Responses

(2010) Ville Härmä et al.   PLoS One

Matrigel: A complex protein mixture required for optimal growth of cell culture

(2010) Chris S. Hughes et al.   PROTEOMICS

Oxygen-Controlled Three-Dimensional Cultures to Analyze Tumor Angiogenesis

(2010) Scott S. Verbridge et al.   TISSUE ENGINEERING PART A

Biomatrices and biomaterials for future developments of bioprinting and biofabrication

(2010) M Nakamura et al.   Biofabrication

Interactions between human osteoblasts and prostate cancer cells in a novel 3D in vitro model

(2010) Shirly Sieh et al.   Organogenesis

Organ printing: Tissue spheroids as building blocks

(2009) Vladimir Mironov et al.   BIOMATERIALS

Microfluidic system for formation of PC-3 prostate cancer co-culture spheroids

(2009) Amy Y. Hsiao et al.   BIOMATERIALS

HER2 signaling pathway activation and response of breast cancer cells to HER2-targeting agents is dependent strongly on the 3D microenvironment

(2009) Britta Weigelt et al.   BREAST CANCER RESEARCH AND TREATMENT

The role of cell adhesion molecules in the progression of colorectal cancer and the development of liver metastasis

(2009) Konstantinos A. Paschos et al.   CELLULAR SIGNALLING

Synthesis of polycaprolactone: a review

(2009) Marianne Labet et al.   CHEMICAL SOCIETY REVIEWS

Genetic and cell biological analysis of integrin outside-in signaling

(2009) K. R. Legate et al.   GENES & DEVELOPMENT

Inhibition of vimentin or  1 integrin reverts morphology of prostate tumor cells grown in laminin-rich extracellular matrix gels and reduces tumor growth in vivo

(2009) X. Zhang et al.   MOLECULAR CANCER THERAPEUTICS

Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche

(2009) Toshiro Sato et al.   NATURE

Nanolitre liquid patterning in aqueous environments for spatially defined reagent delivery to mammalian cells

(2009) H. Tavana et al.   NATURE MATERIALS

Sustained in vitro intestinal epithelial culture within a Wnt-dependent stem cell niche

(2009) Akifumi Ootani et al.   NATURE MEDICINE

Chemotherapy-induced tumor gene expression changes in human breast cancers

(2009) Soo-Chin Lee et al.   Pharmacogenetics and Genomics

Prognostic Breast Cancer Signature Identified from 3D Culture Model Accurately Predicts Clinical Outcome across Independent Datasets

(2008) Katherine J. Martin et al.   PLoS One