Spheroids as a Type of Three-Dimensional Cell Cultures—Examples of Methods of Preparation and the Most Important Application

A novel three-dimensional cell culture method enhances antiviral drug screening in primary human cells

(2018) Robert Koban et al.   ANTIVIRAL RESEARCH

Halfway between 2D and Animal Models: Are 3D Cultures the Ideal Tool to Study Cancer-Microenvironment Interactions?

(2018) Jessica Hoarau-Véchot et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Spheroid-based 3D cell cultures identify salinomycin as a promising drug for the treatment of chondrosarcoma

(2018) Francesca Perut et al.   JOURNAL OF ORTHOPAEDIC RESEARCH

2D versus 3D human induced pluripotent stem cell-derived cultures for neurodegenerative disease modelling

(2018) Eduarda G Z Centeno et al.   Molecular Neurodegeneration

Spheroids-on-a-chip: Recent advances and design considerations in microfluidic platforms for spheroid formation and culture

(2018) Khashayar Moshksayan et al.   SENSORS AND ACTUATORS B-CHEMICAL

Scaffold-free three-dimensional cell culturing using magnetic levitation

(2018) Esra Türker et al.   Biomaterials Science

Multicellular spheroid based on a triple co-culture: A novel 3D model to mimic pancreatic tumor complexity

(2018) Gianpiero Lazzari et al.   Acta Biomaterialia

3D cell coculture tumor model: A promising approach for future cancer drug discovery

(2018) Xin Xin et al.   PROCESS BIOCHEMISTRY

E-cadherin, actin, microtubules and FAK dominate different spheroid formation phases and important elements of tissue integrity

(2018) I. Smyrek et al.   Biology Open

3D Cultures of Parkinson's Disease-Specific Dopaminergic Neurons for High Content Phenotyping and Drug Testing

(2018) Silvia Bolognin et al.   Advanced Science

Advances in multicellular spheroids formation

(2017) X. Cui et al.   Journal of the Royal Society Interface

The SH-SY5Y cell line in Parkinson’s disease research: a systematic review

(2017) Helena Xicoy et al.   Molecular Neurodegeneration

An update on stem cell biology and engineering for brain development

(2017) C J C Parr et al.   MOLECULAR PSYCHIATRY

Application of standard cell cultures and 3D in vitro tissue models as an effective tool in drug design and development

(2017) Aleksandra Amelian et al.   Pharmacological Reports

Application of three-dimensional neuronal cell cultures in the studies of mechanisms of neurodegenerative diseases

(2017) Anna Słońska et al.   Postepy Higieny I Medycyny Doswiadczalnej

Three‑dimensional cell culture: A powerful tool in tumor research and drug discovery (Review)

(2017) Donglai Lv et al.   Oncology Letters

Three-Dimensional Cell Cultures in Drug Discovery and Development

(2017) Ye Fang et al.   SLAS Discovery

An In Vitro Model for Neuroscience: Differentiation of SH-SY5Y Cells into Cells with Morphological and Biochemical Characteristics of Mature Neurons

(2017) Lotta Agholme et al.   JOURNAL OF ALZHEIMERS DISEASE

2D and 3D cell cultures – a comparison of different types of cancer cell cultures

(2016) Marta Kapałczyńska et al.   Archives of Medical Science

Modelling the tumour microenvironment in long-term microencapsulated 3D co-cultures recapitulates phenotypic features of disease progression

(2016) Marta F. Estrada et al.   BIOMATERIALS

3D culture models of Alzheimer’s disease: a road map to a “cure-in-a-dish”

(2016) Se Hoon Choi et al.   Molecular Neurodegeneration

Three-dimensional culture systems in cancer research: Focus on tumor spheroid model

(2016) Sritama Nath et al.   PHARMACOLOGY & THERAPEUTICS

Effects of Polyamidoamine Dendrimers on a 3-D Neurosphere System Using Human Neural Progenitor Cells

(2016) Yang Zeng et al.   TOXICOLOGICAL SCIENCES

Targeting survivin as a potential new treatment for chondrosarcoma of bone

(2016) Y de Jong et al.   Oncogenesis

Bioprinting the Cancer Microenvironment

(2016) Yu Shrike Zhang et al.   ACS Biomaterials-Science & Engineering

Bioprinting the Cancer Microenvironment

(2016) Yu Shrike Zhang et al.   ACS Biomaterials Science & Engineering

Characterization of primary human hepatocyte spheroids as a model system for drug-induced liver injury, liver function and disease

(2016) Catherine C. Bell et al.   Scientific Reports

Alzheimer's in 3D culture: Challenges and perspectives

(2015) Carla D'Avanzo et al.   BIOESSAYS

Three-Dimensional Cell Culture: A Breakthrough in Vivo

(2015) Delphine Antoni et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Differentiation of neuroepithelial stem cells into functional dopaminergic neurons in 3D microfluidic cell culture

(2015) Edinson Lucumi Moreno et al.   LAB ON A CHIP

Dendrimer Nanocarriers for Transport Modulation Across Models of the Pulmonary Epithelium

(2015) Balaji Bharatwaj et al.   MOLECULAR PHARMACEUTICS

The Concept of Prodromal Parkinson’s Disease

(2015) Philipp Mahlknecht et al.   Journal of Parkinsons Disease

MCF-7 Human Breast Cancer Cells Form Differentiated Microtissues in Scaffold-Free Hydrogels

(2015) Marguerite M. Vantangoli et al.   PLoS One

Three-Dimensional Cell Culture Systems and Their Applications in Drug Discovery and Cell-Based Biosensors

(2014) Rasheena Edmondson et al.   ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES

Lessons from a Failed γ-Secretase Alzheimer Trial

(2014) Bart De Strooper   CELL

A critical analysis of the ‘amyloid cascade hypothesis’

(2014) Richard A. Armstrong   FOLIA NEUROPATHOLOGICA

Phaseguides as tunable passive microvalves for liquid routing in complex microfluidic networks

(2014) Ender Yildirim et al.   LAB ON A CHIP

A microfluidic platform for chemoresistive testing of multicellular pleural cancer spheroids

(2014) Janine Ruppen et al.   LAB ON A CHIP

The hallmarks of Parkinson's disease

(2013) Paul M. A. Antony et al.   FEBS Journal

Microfluidic titer plate for stratified 3D cell culture

(2013) Sebastiaan J. Trietsch et al.   LAB ON A CHIP

Spheroid-based three-dimensional liver-on-a-chip to investigate hepatocyte–hepatic stellate cell interactions and flow effects

(2013) Seung-A Lee et al.   LAB ON A CHIP

Impact of the 3D Microenvironment on Phenotype, Gene Expression, and EGFR Inhibition of Colorectal Cancer Cell Lines

(2013) Anna C. Luca et al.   PLoS One

Dendrimers as Carriers for siRNA Delivery and Gene Silencing: A Review

(2013) Jiangyu Wu et al.   TheScientificWorldJOURNAL

An oxygen-permeable spheroid culture system for the prevention of central hypoxia and necrosis of spheroids

(2012) Takahisa Anada et al.   BIOMATERIALS

Three-dimensional cell culture: the missing link in drug discovery

(2012) Susan Breslin et al.   DRUG DISCOVERY TODAY

Loss of cancer drug activity in colon cancer HCT-116 cells during spheroid formation in a new 3-D spheroid cell culture system

(2012) Henning Karlsson et al.   EXPERIMENTAL CELL RESEARCH

Induced Tauopathy in a Novel 3D-Culture Model Mediates Neurodegenerative Processes: A Real-Time Study on Biochips

(2012) Diana Seidel et al.   PLoS One

Efficient formation of cell spheroids using polymer nanofibers

(2011) Jung-Youn Shin et al.   BIOTECHNOLOGY LETTERS

Phaseguides: a paradigm shift in microfluidic priming and emptying

(2011) Paul Vulto et al.   LAB ON A CHIP

The Role of Autophagy in Cancer: Therapeutic Implications

(2011) Z. J. Yang et al.   MOLECULAR CANCER THERAPEUTICS

Priorities in Parkinson's disease research

(2011) Wassilios G. Meissner et al.   NATURE REVIEWS DRUG DISCOVERY

MCF-7 Cells as a Three-Dimensional Model for the Study of Human Breast Cancer

(2011) Jônatas Bussador do Amaral et al.   TISSUE ENGINEERING PART C-METHODS

A Simple Hanging Drop Cell Culture Protocol for Generation of 3D Spheroids

(2011) Ramsey Foty   Jove-Journal of Visualized Experiments

High-throughput 3D spheroid culture and drug testing using a 384 hanging drop array

(2010) Yi-Chung Tung et al.   ANALYST

Inhibition of poxvirus spreading by the anti-tumor drug Gefitinib (Iressa™)

(2010) Stefan Langhammer et al.   ANTIVIRAL RESEARCH

DAMP-mediated autophagy contributes to drug resistance

(2010) Liying Liu et al.   Autophagy

Therapeutic molecular targets in human chondrosarcoma

(2010) Nuor Jamil et al.   INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY

Multicellular tumor spheroids: An underestimated tool is catching up again

(2010) Franziska Hirschhaeuser et al.   JOURNAL OF BIOTECHNOLOGY

Three-dimensional tissue culture based on magnetic cell levitation

(2010) Glauco R. Souza et al.   Nature Nanotechnology

Engineering a scaffold-free 3D tumor model for in vitro drug penetration studies

(2009) Siew-Min Ong et al.   BIOMATERIALS

In vitro Toxicity Testing of Nanoparticles in 3D Cell Culture

(2009) Jungwoo Lee et al.   Small

3-D Tissue Culture Systems for the Evaluation and Optimization of Nanoparticle-Based Drug Carriers

(2008) Thomas Tyrel Goodman et al.   BIOCONJUGATE CHEMISTRY

Recent advances in three-dimensional multicellular spheroid culture for biomedical research

(2008) Ruei-Zhen Lin et al.   Biotechnology Journal

Thirty years of Alzheimer's disease genetics: the implications of systematic meta-analyses

(2008) Lars Bertram et al.   NATURE REVIEWS NEUROSCIENCE