Fast and efficient generation of knock-in human organoids using homology-independent CRISPR–Cas9 precision genome editing

Precise Gene Editing Preserves Hematopoietic Stem Cell Function following Transient p53-Mediated DNA Damage Response

(2019) Giulia Schiroli et al.   Cell Stem Cell

A Human iPSC Double-Reporter System Enables Purification of Cardiac Lineage Subpopulations with Distinct Function and Drug Response Profiles

(2019) Joe Z. Zhang et al.   Cell Stem Cell

Identification of Enteroendocrine Regulators by Real-Time Single-Cell Differentiation Mapping

(2019) Helmuth Gehart et al.   CELL

Probing the Tumor Suppressor Function of BAP1 in CRISPR-Engineered Human Liver Organoids

(2019) Benedetta Artegiani et al.   Cell Stem Cell

Self-organization and symmetry breaking in intestinal organoid development

(2019) Denise Serra et al.   NATURE

Reconstruction of the Human Colon Epithelium In Vivo

(2018) Shinya Sugimoto et al.   Cell Stem Cell

Use and application of 3D-organoid technology

(2018) Benedetta Artegiani et al.   HUMAN MOLECULAR GENETICS

Enteroendocrine cells switch hormone expression along the crypt-to-villus BMP signalling gradient

(2018) Joep Beumer et al.   NATURE CELL BIOLOGY

p53 inhibits CRISPR–Cas9 engineering in human pluripotent stem cells

(2018) Robert J. Ihry et al.   NATURE MEDICINE

CRISPR–Cas9 genome editing induces a p53-mediated DNA damage response

(2018) Emma Haapaniemi et al.   NATURE MEDICINE

Chromosome Segregation Fidelity in Epithelia Requires Tissue Architecture

(2018) Kristin A. Knouse et al.   CELL

Progress and potential in organoid research

(2018) Giuliana Rossi et al.   NATURE REVIEWS GENETICS

Long-Term Expansion of Functional Mouse and Human Hepatocytes as 3D Organoids

(2018) Huili Hu et al.   CELL

Human Intestinal Organoids Maintain Self-Renewal Capacity and Cellular Diversity in Niche-Inspired Culture Condition

(2018) Masayuki Fujii et al.   Cell Stem Cell

Cell–cell adhesion accounts for the different orientation of columnar and hepatocytic cell divisions

(2017) Francisco Lázaro-Diéguez et al.   JOURNAL OF CELL BIOLOGY

Visualization and targeting of LGR5+ human colon cancer stem cells

(2017) Mariko Shimokawa et al.   NATURE

Surrogate Wnt agonists that phenocopy canonical Wnt and β-catenin signalling

(2017) Claudia Y. Janda et al.   NATURE

Cell competition with normal epithelial cells promotes apical extrusion of transformed cells through metabolic changes

(2017) Shunsuke Kon et al.   NATURE CELL BIOLOGY

The control of DNA repair by the cell cycle

(2017) Nicole Hustedt et al.   NATURE CELL BIOLOGY

Use of CRISPR-modified human stem cell organoids to study the origin of mutational signatures in cancer

(2017) Jarno Drost et al.   SCIENCE

Efficient Precision Genome Editing in iPSCs via Genetic Co-targeting with Selection

(2017) Katie A. Mitzelfelt et al.   Stem Cell Reports

Modeling Development and Disease with Organoids

(2016) Hans Clevers   CELL

Replacement of Lost Lgr5-Positive Stem Cells through Plasticity of Their Enterocyte-Lineage Daughters

(2016) Paul W. Tetteh et al.   Cell Stem Cell

Visualization of a short-range Wnt gradient in the intestinal stem-cell niche

(2016) Henner F. Farin et al.   NATURE

In vivo genome editing via CRISPR/Cas9 mediated homology-independent targeted integration

(2016) Keiichiro Suzuki et al.   NATURE

Knock-in of large reporter genes in human cells via CRISPR/Cas9-induced homology-dependent and independent DNA repair

(2016) Xiangjun He et al.   NUCLEIC ACIDS RESEARCH

Long-Term Culture of Genome-Stable Bipotent Stem Cells from Adult Human Liver

(2015) Meritxell Huch et al.   CELL

Sequential cancer mutations in cultured human intestinal stem cells

(2015) Jarno Drost et al.   NATURE

Modeling colorectal cancer using CRISPR-Cas9–mediated engineering of human intestinal organoids

(2015) Mami Matano et al.   NATURE MEDICINE

Efficient genetic engineering of human intestinal organoids using electroporation

(2015) Masayuki Fujii et al.   Nature Protocols

A generic strategy for CRISPR-Cas9-mediated gene tagging

(2015) Daniel H. Lackner et al.   Nature Communications

Cytokinesis defines a spatial landmark for hepatocyte polarization and apical lumen formation

(2014) T. Wang et al.   JOURNAL OF CELL SCIENCE

Is Non-Homologous End-Joining Really an Inherently Error-Prone Process?

(2014) Mireille Bétermier et al.   PLoS Genetics

Functional Repair of CFTR by CRISPR/Cas9 in Intestinal Stem Cell Organoids of Cystic Fibrosis Patients

(2013) Gerald Schwank et al.   Cell Stem Cell

Highly efficient CRISPR/Cas9-mediated knock-in in zebrafish by homology-independent DNA repair

(2013) T. O. Auer et al.   GENOME RESEARCH

p53 regulates a mitotic transcription program and determines ploidy in normal mouse liver

(2013) Svitlana Kurinna et al.   HEPATOLOGY

Niche-independent high-purity cultures of Lgr5+ intestinal stem cells and their progeny

(2013) Xiaolei Yin et al.   NATURE METHODS

Genome engineering using the CRISPR-Cas9 system

(2013) F Ann Ran et al.   Nature Protocols

Aneuploidy as a mechanism for stress-induced liver adaptation

(2012) Andrew W. Duncan et al.   JOURNAL OF CLINICAL INVESTIGATION

An Efficient Nonviral Method to Generate Integration-Free Human-Induced Pluripotent Stem Cells from Cord Blood and Peripheral Blood Cells

(2012) Keisuke Okita et al.   STEM CELLS

Long-term Expansion of Epithelial Organoids From Human Colon, Adenoma, Adenocarcinoma, and Barrett's Epithelium

(2011) Toshiro Sato et al.   GASTROENTEROLOGY

Frequent Aneuploidy Among Normal Human Hepatocytes

(2011) Andrew W. Duncan et al.   GASTROENTEROLOGY

p53: Guardian of ploidy

(2011) Yael Aylon et al.   Molecular Oncology

The ploidy conveyor of mature hepatocytes as a source of genetic variation

(2010) Andrew W. Duncan et al.   NATURE