Interplay of cGAS with micronuclei: regulation and diseases

The cGAS–STING pathway drives type I IFN immunopathology in COVID-19

(2022) Jeremy Di Domizio et al.   NATURE

NBS1-CtIP–mediated DNA end resection suppresses cGAS binding to micronuclei

(2022) Salim Abdisalaam et al.   NUCLEIC ACIDS RESEARCH

SARS-CoV-2 infection induces a pro-inflammatory cytokine response through cGAS-STING and NF-κB

(2022) Christopher J. Neufeldt et al.   Communications Biology

DNA damage contributes to neurotoxic inflammation in Aicardi-Goutières syndrome astrocytes

(2022) Anna Maria Sole Giordano et al.   JOURNAL OF EXPERIMENTAL MEDICINE

Somatic genomic changes in single Alzheimer’s disease neurons

(2022) Michael B. Miller et al.   NATURE

Breakage of cytoplasmic chromosomes by pathological DNA base excision repair

(2022) Shangming Tang et al.   NATURE

STING mediates neurodegeneration and neuroinflammation in nigrostriatal α-synucleinopathy

(2022) Jared T. Hinkle et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

SARS-CoV-2 spike protein–induced cell fusion activates the cGAS-STING pathway and the interferon response

(2022) Xiaoman Liu et al.   Science Signaling

MARCH8 attenuates cGAS-mediated innate immune responses through ubiquitylation

(2022) Xikang Yang et al.   Science Signaling

Tracing brain genotoxic stress in Parkinson’s disease with a novel single-cell genetic sensor

(2022) Madison Wynne El-Saadi et al.   Science Advances

ER-directed TREX1 limits cGAS activation at micronuclei

(2021) Lisa Mohr et al.   MOLECULAR CELL

A Nuclear Export Signal Is Required for cGAS to Sense Cytosolic DNA

(2021) Hong Sun et al.   Cell Reports

cGAS phase separation inhibits TREX1-mediated DNA degradation and enhances cytosolic DNA sensing

(2021) Wen Zhou et al.   MOLECULAR CELL

Nuclear sensing of breaks in mitochondrial DNA enhances immune surveillance

(2021) Marco Tigano et al.   NATURE

Phosphorylation and chromatin tethering prevent cGAS activation during mitosis

(2021) Tuo Li et al.   SCIENCE

Disruption of DNA polymerase ζ engages an innate immune response

(2021) Sara K. Martin et al.   Cell Reports

Drugs that inhibit TMEM16 proteins block SARS-CoV-2 spike-induced syncytia

(2021) Luca Braga et al.   NATURE

Di-phosphorylated BAF shows altered structural dynamics and binding to DNA, but interacts with its nuclear envelope partners

(2021) Agathe Marcelot et al.   NUCLEIC ACIDS RESEARCH

cGAS–STING cytosolic DNA sensing pathway is suppressed by JAK2-STAT3 in tumor cells

(2021) Manuel Adrian Suter et al.   Scientific Reports

Decoding and rejuvenating human ageing genomes: Lessons from mosaic chromosomal alterations

(2021) Xueqin Dai et al.   AGEING RESEARCH REVIEWS

Chromosome aberrations induced by the non-mutagenic carcinogen acetamide involve in rat hepatocarcinogenesis through micronucleus formation in hepatocytes

(2021) Kenji Nakamura et al.   ARCHIVES OF TOXICOLOGY

Aneuploid senescent cells activate NF‐κB to promote their immune clearance by NK cells

(2021) Ruoxi W Wang et al.   EMBO REPORTS

Micronuclei in Kif18a mutant mice form stable micronuclear envelopes and do not promote tumorigenesis

(2021) Leslie A. Sepaniac et al.   JOURNAL OF CELL BIOLOGY

Nuclear compartmentalization as a mechanism of quantitative control of gene expression

(2021) Prashant Bhat et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Peripheral and central immune system crosstalk in Alzheimer disease — a research prospectus

(2021) Brianne M. Bettcher et al.   Nature Reviews Neurology

NAD+ supplementation reduces neuroinflammation and cell senescence in a transgenic mouse model of Alzheimer’s disease via cGAS–STING

(2021) Yujun Hou et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cytosolic dsDNA of mitochondrial origin induces cytotoxicity and neurodegeneration in cellular and zebrafish models of Parkinson’s disease

(2021) Hideaki Matsui et al.   Nature Communications

Autophagy-Mediated Clearance of Free Genomic DNA in the Cytoplasm Protects the Growth and Survival of Cancer Cells

(2021) Mengfei Yao et al.   Frontiers in Oncology

Genotoxic stress in constitutive trisomies induces autophagy and the innate immune response via the cGAS-STING pathway

(2021) Maria Krivega et al.   Communications Biology

Micronucleus production, activation of DNA damage response and cGAS-STING signaling in syncytia induced by SARS-CoV-2 infection

(2021) He Ren et al.   Biology Direct

Cytoplasmic DNA: sources, sensing, and role in aging and disease

(2021) Karl N. Miller et al.   CELL

DNA hypomethylation leads to cGAS‐induced autoinflammation in the epidermis

(2021) Mirjam A Beck et al.   EMBO JOURNAL

Paclitaxel Induces Micronucleation and Activates Pro-Inflammatory cGAS–STING Signaling in Triple-Negative Breast Cancer

(2021) Yang Hu et al.   MOLECULAR CANCER THERAPEUTICS

Folate deficiency enhances the in vitro genotoxicity of bile acids in human colon and liver cells

(2021) Jianfei Li et al.   MUTAGENESIS

eccDNAs are apoptotic products with high innate immunostimulatory activity

(2021) Yuangao Wang et al.   NATURE

Chromatin bridges, not micronuclei, activate cGAS after drug-induced mitotic errors in human cells

(2021) Patrick J. Flynn et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Mitochondrial DNA: cellular genotoxic stress sentinel

(2021) Zheng Wu et al.   TRENDS IN BIOCHEMICAL SCIENCES

An anaphase surveillance mechanism prevents micronuclei formation from frequent chromosome segregation errors

(2021) Bernardo Orr et al.   Cell Reports

Mosaic loss of human Y chromosome: what, how and why

(2020) Xihan Guo et al.   HUMAN GENETICS

Autolysosomal degradation of cytosolic chromatin fragments antagonizes oxidative stress–induced senescence

(2020) Xiaojuan Han et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

STING-dependent paracriny shapes apoptotic priming of breast tumors in response to anti-mitotic treatment

(2020) Steven Lohard et al.   Nature Communications

Topoisomerase 1 cleavage complex enables pattern recognition and inflammation during senescence

(2020) Bo Zhao et al.   Nature Communications

USP29 maintains the stability of cGAS and promotes cellular antiviral responses and autoimmunity

(2020) Qiang Zhang et al.   CELL RESEARCH

Molecular mechanisms and cellular functions of cGAS–STING signalling

(2020) Karl-Peter Hopfner et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Combination of CHEK1/2 inhibition and ionizing radiation results in abscopal tumor response through increased micronuclei formation

(2020) Hann-Hsiang Chao et al.   ONCOGENE

Mechanisms generating cancer genome complexity from a single cell division error

(2020) Neil T. Umbreit et al.   SCIENCE

Metabolic reprogramming and cancer progression

(2020) Brandon Faubert et al.   SCIENCE

Barrier-to-Autointegration Factor 1 Protects against a Basal cGAS-STING Response

(2020) Hongming Ma et al.   mBio

Phosphorylation of cGAS by CDK1 impairs self-DNA sensing in mitosis

(2020) Li Zhong et al.   Cell Discovery

Old dogs, new trick: classic cancer therapies activate cGAS

(2020) Seoyun Yum et al.   CELL RESEARCH

Understanding the birth of rupture-prone and irreparable micronuclei

(2020) Xihan Guo et al.   CHROMOSOMA

Structures and Mechanisms in the cGAS-STING Innate Immunity Pathway

(2020) Xuewu Zhang et al.   IMMUNITY

Unrestrained ESCRT-III drives micronuclear catastrophe and chromosome fragmentation

(2020) Marina Vietri et al.   NATURE CELL BIOLOGY

Cyclic GMP-AMP synthase promotes the inflammatory and autophagy responses in Huntington disease

(2020) Manish Sharma et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Bacterial-induced cell fusion is a danger signal triggering cGAS–STING pathway via micronuclei formation

(2020) Joanne Wei Kay Ku et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Regulation and Consequences of cGAS Activation by Self-DNA

(2020) Christian Zierhut et al.   TRENDS IN CELL BIOLOGY

Is a “Cytokine Storm” Relevant to COVID-19?

(2020) Pratik Sinha et al.   JAMA Internal Medicine

TDP-43 Triggers Mitochondrial DNA Release via mPTP to Activate cGAS/STING in ALS

(2020) Chien-Hsiung Yu et al.   CELL

Structural basis for nucleosome-mediated inhibition of cGAS activity

(2020) Duanfang Cao et al.   CELL RESEARCH

Syncytia formation by SARS‐CoV‐2 infected cells

(2020) Julian Buchrieser et al.   EMBO JOURNAL

Alerting the immune system to DNA damage: micronuclei as mediators

(2020) Kate M. MacDonald et al.   Essays in Biochemistry

Small but strong: Mutational and functional landscapes of micronuclei in cancer genomes

(2020) Xihan Guo et al.   INTERNATIONAL JOURNAL OF CANCER

Diverse cellular functions of barrier-to-autointegration factor and its roles in disease

(2020) Rhiannon M. Sears et al.   JOURNAL OF CELL SCIENCE

Micronuclei, inflammation and auto-immune disease

(2020) Micheline Kirsch-Volders et al.   MUTATION RESEARCH-REVIEWS IN MUTATION RESEARCH

The Molecular Basis of Tight Nuclear Tethering and Inactivation of cGAS

(2020) Baoyu Zhao et al.   NATURE

Structural basis for sequestration and autoinhibition of cGAS by chromatin

(2020) Sebastian Michalski et al.   NATURE

Structural mechanism of cGAS inhibition by the nucleosome

(2020) Ganesh R. Pathare et al.   NATURE

BAF restricts cGAS on nuclear DNA to prevent innate immune activation

(2020) Baptiste Guey et al.   SCIENCE

Structural basis for the inhibition of cGAS by nucleosomes

(2020) Tomoya Kujirai et al.   SCIENCE

Structural basis of nucleosome-dependent cGAS inhibition

(2020) Joshua A. Boyer et al.   SCIENCE

Cell Cycle Checkpoints Cooperate to Suppress DNA- and RNA-Associated Molecular Pattern Recognition and Anti-Tumor Immune Responses

(2020) Jie Chen et al.   Cell Reports

cGAS suppresses genomic instability as a decelerator of replication forks

(2020) Hao Chen et al.   Science Advances

Chromothripsis drives the evolution of gene amplification in cancer

(2020) Ofer Shoshani et al.   NATURE

cGAS-mediated induction of type I interferon due to inborn errors of histone pre-mRNA processing

(2020) Carolina Uggenti et al.   NATURE GENETICS

Metastasis and immune evasion from extracellular cGAMP hydrolysis

(2020) Jun Li et al.   Cancer Discovery

Signaling by cGAS–STING in Neurodegeneration, Neuroinflammation, and Aging

(2020) Bindu D. Paul et al.   TRENDS IN NEUROSCIENCES

High Concentration of Sugars is Genotoxic in Folate Deficient Cells

(2019) Xihan Guo et al.   MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS

BRCA2 deficiency instigates cGAS-mediated inflammatory signaling and confers sensitivity to tumor necrosis factor-alpha-mediated cytotoxicity

(2019) Anne Margriet Heijink et al.   Nature Communications

The N-Terminal Domain of cGAS Determines Preferential Association with Centromeric DNA and Innate Immune Activation in the Nucleus

(2019) Matteo Gentili et al.   Cell Reports

cGAS in action: Expanding roles in immunity and inflammation

(2019) Andrea Ablasser et al.   SCIENCE

L1 drives IFN in senescent cells and promotes age-associated inflammation

(2019) Marco De Cecco et al.   NATURE

Acetylation Blocks cGAS Activity and Inhibits Self-DNA-Induced Autoimmunity

(2019) Jiang Dai et al.   CELL

Phosphoinositide Interactions Position cGAS at the Plasma Membrane to Ensure Efficient Distinction between Self- and Viral DNA

(2019) Katherine C. Barnett et al.   CELL

Bloom syndrome protein restrains innate immune sensing of micronuclei by cGAS

(2019) Matthieu Gratia et al.   JOURNAL OF EXPERIMENTAL MEDICINE

Micronuclei and Genome Chaos: Changing the System Inheritance

(2019) Christine J. Ye et al.   Genes

ESCRT-III is necessary for the integrity of the nuclear envelope in micronuclei but is aberrant at ruptured micronuclear envelopes generating damage

(2019) Jessica Willan et al.   Oncogenesis

The Cytoplasmic DNA Sensor cGAS Promotes Mitotic Cell Death

(2019) Christian Zierhut et al.   CELL

DNA sensing by the cGAS–STING pathway in health and disease

(2019) Mona Motwani et al.   NATURE REVIEWS GENETICS

Chromatin‐bound cGAS is an inhibitor of DNA repair and hence accelerates genome destabilization and cell death

(2019) Hui Jiang et al.   EMBO JOURNAL

Circular ecDNA promotes accessible chromatin and high oncogene expression

(2019) Sihan Wu et al.   NATURE

Micronuclei-based model system reveals functional consequences of chromothripsis in human cells

(2019) Maja Kneissig et al.   eLife

TDP-43 aggregation inside micronuclei reveals a potential mechanism for protein inclusion formation in ALS

(2019) Cristian A. Droppelmann et al.   Scientific Reports

Tight nuclear tethering of cGAS is essential for preventing autoreactivity

(2019) Hannah E Volkman et al.   eLife

LINE1 contributes to autoimmunity through both RIG-I- and MDA5-mediated RNA sensing pathways

(2018) Ke Zhao et al.   JOURNAL OF AUTOIMMUNITY

CLIP and cohibin separate rDNA from nucleolar proteins destined for degradation by nucleophagy

(2018) Md. Golam Mostofa et al.   JOURNAL OF CELL BIOLOGY

Differences in the Effects of EGCG on Chromosomal Stability and Cell Growth between Normal and Colon Cancer Cells

(2018) Juan Ni et al.   MOLECULES

OUP accepted manuscript

(2018)   MUTAGENESIS

Chromosomal instability drives metastasis through a cytosolic DNA response

(2018) Samuel F. Bakhoum et al.   NATURE

DNA-induced liquid phase condensation of cGAS activates innate immune signaling

(2018) Mingjian Du et al.   SCIENCE

Radiation-Induced Chromosomal Aberrations and Immunotherapy: Micronuclei, Cytosolic DNA, and Interferon-Production Pathway

(2018) Marco Durante et al.   Frontiers in Oncology

Parkin and PINK1 mitigate STING-induced inflammation

(2018) Danielle A. Sliter et al.   NATURE

Nuclear envelope assembly defects link mitotic errors to chromothripsis

(2018) Shiwei Liu et al.   NATURE

Geraniin Differentially Modulates Chromosome Stability of Colon Cancer and Noncancerous Cells by Oppositely Regulating their Spindle Assembly Checkpoint

(2018) Xihan Guo et al.   ENVIRONMENTAL AND MOLECULAR MUTAGENESIS

The molecular origins and pathophysiological consequences of micronuclei: New insights into an age-old problem

(2018) Xihan Guo et al.   MUTATION RESEARCH-REVIEWS IN MUTATION RESEARCH

Nuclear cGAS suppresses DNA repair and promotes tumorigenesis

(2018) Haipeng Liu et al.   NATURE

Absence of RNase H2 triggers generation of immunogenic micronuclei removed by autophagy

(2017) Kareen Bartsch et al.   HUMAN MOLECULAR GENETICS

Folate deficiency induces mitotic aberrations and chromosomal instability by compromising the spindle assembly checkpoint in cultured human colon cells

(2017) Xihan Guo et al.   MUTAGENESIS

Fate of micronuclei and micronucleated cells

(2017) Henning Hintzsche et al.   MUTATION RESEARCH-REVIEWS IN MUTATION RESEARCH

cGAS surveillance of micronuclei links genome instability to innate immunity

(2017) Karen J. Mackenzie et al.   NATURE

Mitotic progression following DNA damage enables pattern recognition within micronuclei

(2017) Shane M. Harding et al.   NATURE

Cytoplasmic chromatin triggers inflammation in senescence and cancer

(2017) Zhixun Dou et al.   NATURE

cGAS senses long and HMGB/TFAM-bound U-turn DNA by forming protein–DNA ladders

(2017) Liudmila Andreeva et al.   NATURE

Innate immune sensing of cytosolic chromatin fragments through cGAS promotes senescence

(2017) Selene Glück et al.   NATURE CELL BIOLOGY

cGAS is essential for cellular senescence

(2017) Hui Yang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

DNA exonuclease Trex1 regulates radiotherapy-induced tumour immunogenicity

(2017) Claire Vanpouille-Box et al.   Nature Communications

Mislocalization of centromeric histone H3 variant CENP-A contributes to chromosomal instability (CIN) in human cells

(2017) Roshan L. Shrestha et al.   Oncotarget

Selective Y centromere inactivation triggers chromosome shattering in micronuclei and repair by non-homologous end joining

(2016) Peter Ly et al.   NATURE CELL BIOLOGY

Glutamylation of the DNA sensor cGAS regulates its binding and synthase activity in antiviral immunity

(2016) Pengyan Xia et al.   NATURE IMMUNOLOGY

ESCRT III repairs nuclear envelope ruptures during cell migration to limit DNA damage and cell death

(2016) M. Raab et al.   SCIENCE

Nuclear envelope rupture and repair during cancer cell migration

(2016) C. M. Denais et al.   SCIENCE

Chromothripsis and Kataegis Induced by Telomere Crisis

(2015) John Maciejowski et al.   CELL

Cutting Edge: cGAS Is Required for Lethal Autoimmune Disease in the Trex1-Deficient Mouse Model of Aicardi–Goutières Syndrome

(2015) Elizabeth E. Gray et al.   JOURNAL OF IMMUNOLOGY

Chromothripsis from DNA damage in micronuclei

(2015) Cheng-Zhong Zhang et al.   NATURE

Akt Kinase-Mediated Checkpoint of cGAS DNA Sensing Pathway

(2015) Gil Ju Seo et al.   Cell Reports

Phyllanthus emblica L. Fruit Extract Induces Chromosomal Instability and Suppresses Necrosis in Human Colon Cancer Cells

(2014) Xihan Guo et al.   INTERNATIONAL JOURNAL FOR VITAMIN AND NUTRITION RESEARCH

TREX1 Deficiency Triggers Cell-Autonomous Immunity in a cGAS-Dependent Manner

(2014) A. Ablasser et al.   JOURNAL OF IMMUNOLOGY

Arginine starvation-associated atypical cellular death involves mitochondrial dysfunction, nuclear DNA leakage, and chromatin autophagy

(2014) Chun A. Changou et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Catastrophic Nuclear Envelope Collapse in Cancer Cell Micronuclei

(2013) Emily M. Hatch et al.   CELL

Lysosome-mediated processing of chromatin in senescence

(2013) Andre Ivanov et al.   JOURNAL OF CELL BIOLOGY

Replication stress links structural and numerical cancer chromosomal instability

(2013) Rebecca A. Burrell et al.   NATURE

Autophagic removal of micronuclei

(2012) Santiago Rello-Varona et al.   CELL CYCLE

Folate deficiency enhances the inflammatory response of macrophages

(2012) Andreas F. Kolb et al.   MOLECULAR IMMUNOLOGY

DNA breaks and chromosome pulverization from errors in mitosis

(2012) Karen Crasta et al.   NATURE

Cyclic GMP-AMP Synthase Is a Cytosolic DNA Sensor That Activates the Type I Interferon Pathway

(2012) L. Sun et al.   SCIENCE

The dynamics of the nucleosome: thermal effects, external forces and ATP

(2011) Ralf Blossey et al.   FEBS Journal

Force and Length in the Mitotic Spindle

(2009) Sophie Dumont et al.   CURRENT BIOLOGY

Trex1 Prevents Cell-Intrinsic Initiation of Autoimmunity

(2008) Daniel B. Stetson et al.   CELL