Article
Cell Biology
Pingping Wang, Lifen Yao, Meng Luo, Wenyang Zhou, Xiyun Jin, Zhaochun Xu, Shi Yan, Yiqun Li, Chang Xu, Rui Cheng, Yan Huang, Xiaoyu Lin, Kexin Ma, Huimin Cao, Hongxin Liu, Guangfu Xue, Fang Han, Huan Nie, Qinghua Jiang
Summary: The study reveals the presence of a large population of CD8(+) T cells progressing continuously and remarkably expanded CD4 CTLs in PD patients through TCR tracking. Additionally, potential TCR-antigen pairs were identified, providing evidence for peripheral T cells to participate in neuronal degeneration.
Article
Immunology
Qing Guan, Wei Liu, Ketao Mu, Qi Hu, Jiazhao Xie, Liming Cheng, Xiong Wang
Summary: Brain infiltration of NK cells has been observed in neurodegenerative disorders, including Parkinson's disease. This study investigates the molecular mechanisms of NK cell infiltration in PD and identifies RAC1 as a key gene involved in this process and neuroprotection.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Cell Biology
Cheng Chen, Yuanxin Liao, Guangdun Peng
Summary: Single-cell lineage tracing, a fundamental goal in modern biology, allows the depiction of cells' history, state, and fate. Various strategies, including integration barcodes, polylox barcodes, and CRISPR barcodes, have been developed to achieve single-cell lineage tracing at high resolution. Despite their successful application in animal development and stem cell research, careful implementation and effort are still required.
Article
Multidisciplinary Sciences
Valeria Bonapersona, Heike Schuler, Ruth Damsteegt, Youri Adolfs, R. Jeroen Pasterkamp, Martijn P. van den Heuvel, Marian Joels, R. Angela Sarabdjitsingh
Summary: Acute stress leads to sequential activation of functional brain networks, resulting in changes in cellular activity. The study found that areas such as the hypothalamus, amygdala, prefrontal cortex, hippocampus, and thalamus are most affected by acute stress. The cellular activity patterns also shift over time, and the strategy of some brain areas changes after stress.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Editorial Material
Neurosciences
Ernest Arenas
Summary: This study reveals the existence of ten distinct dopamine-producing neuron subpopulations in the substantia nigra, but only one of them is associated with significant PD genetic risk and vulnerable to neurodegeneration in PD.
NATURE NEUROSCIENCE
(2022)
Review
Immunology
Seungyoul Oh, Daniel H. D. Gray, Mark M. W. Chong
Summary: T cell development occurs in the thymus, where progenitors differentiate into a range of sublineages with distinct functions. By utilizing single-cell RNA sequencing approaches, researchers aim to gain unprecedented insight into the identity of cells and their gene expression programs, allowing for a deeper understanding of T cell development.
JOURNAL OF IMMUNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Shi-Xun Ma, Su Bin Lim
Summary: Single-cell and single-nucleus RNA sequencing technologies have advanced the molecular pathogenesis research of neurodegenerative disorders, particularly Parkinson's disease. Despite technical challenges in PD, recent studies have successfully characterized diverse cell-type populations and identified cell type-specific disease associations in PD.
Article
Biology
Sophie Seidel, Tanja Stadler
Summary: This study proposes a phylodynamic inference approach based on single-cell lineage recorder data to estimate time-scaled single-cell trees and cell population dynamics. The performance and comparability of the method are validated and benchmarked against state-of-the-art methods. The practical application of TiDeTree is demonstrated using a public dataset.
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2022)
Article
Neurosciences
Tushar Kamath, Abdulraouf Abdulraouf, S. J. Burris, Jonah Langlieb, Vahid Gazestani, Naeem M. Nadaf, Karol Balderrama, Charles Vanderburg, Evan Z. Macosko
Summary: In this study, single-cell genomics was used to analyze thousands of human dopamine neurons and identify a unique population susceptible to Parkinson's disease, which was enriched for genetic risk.
NATURE NEUROSCIENCE
(2022)
Article
Multidisciplinary Sciences
Iain C. Clark, Cristina Gutierrez-Vazquez, Michael A. Wheeler, Zhaorong Li, Veit Rothhammer, Mathias Linnerbauer, Liliana M. Sanmarco, Lydia Guo, Manon Blain, Stephanie E. J. Zandee, Chun-Cheih Chao, Katelyn Batterman, Marius Schwabenland, Peter Lotfy, Amalia Tejeda-Velarde, Patrick Hewson, Carolina Manganeli Polonio, Michael W. Shultis, Yasmin Salem, Emily C. Tjon, Pedro H. Fonseca-Castro, Davis M. Borucki, Kalil Alves de Lima, Agustin Plasencia, Adam R. Abate, Douglas L. Rosene, Kevin J. Hodgetts, Marco Prinz, Jack P. Antel, Alexandre Prat, Francisco J. Quintana
Summary: The study identified microglia-astrocyte interactions and candidate therapeutic targets using RABID-seq, providing new insights into CNS pathology.
Article
Biology
Gabriela Novak, Dimitrios Kyriakis, Kamil Grzyb, Michela Bernini, Sophie Rodius, Gunnar Dittmar, Steven Finkbeiner, Alexander Skupin
Summary: This study used single-cell RNA sequencing and gene expression analysis to investigate the differentiation of midbrain dopaminergic neurons in Parkinson's disease. The results revealed a core network of genes that interact with pathways associated with Parkinson's disease. These findings are important for understanding the pathology and phenotypic heterogeneity of Parkinson's disease.
COMMUNICATIONS BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Fabian Klostermann, Moritz Boll, Felicitas Ehlen, Hannes Ole Tiedt, Michelle Wyrobnik
Summary: This study investigated the changes in verb generation ability and semantic relevance in patients with Parkinson's disease (PD) during a language production task. The results showed that PD patients had reduced verb production and lower semantic relevance to body movements. This suggests an impact of motor system states on lexical processing.
SCIENTIFIC REPORTS
(2022)
Article
Toxicology
Nancy B. Andrioli, Gabriela Chaufan
Summary: The study evaluated the genotoxicity and oxidative stress in the A549 cell line exposed to sublethal concentrations of Iprodione. The increase in Iprodione concentrations slightly raised the probability of belonging to the genotoxic category, while oxidative stress analysis showed a significant linear increase in SOD and a significant linear decrease in GSH and GST.
ARCHIVES OF TOXICOLOGY
(2021)
Article
Multidisciplinary Sciences
Parker C. Wilson, Yoshiharu Muto, Haojia Wu, Anil Karihaloo, Sushrut S. Waikar, Benjamin D. Humphreys
Summary: This study demonstrates that diabetic kidney disease leads to reduced accessibility of glucocorticoid receptor binding sites and injury-associated expression signature in the proximal tubule. The researchers propose that chromatin accessibility is regulated by genetic background and closely intertwined with metabolic memory.
NATURE COMMUNICATIONS
(2022)
Article
Developmental Biology
Miquel Sendra, Juan de Dios Hourcade, Susana Temino, Antonio J. Sarabia, Oscar H. Ocana, Miguel Torres, Jorge N. Dominguez
Summary: Tracing and manipulating cells in embryos are essential to understand development. Lipophilic dye microinjections, viral transfection, and iontophoresis have been key techniques for mapping the origin of progenitor cells. Here, the researchers used cell-permeant Cre recombinase microinjection to perform lineage tracing and gene ablation. The tool will help identify cell progenitors and gene networks involved in organ development and understand the origin of congenital defects.
Article
Cell Biology
Ruijie Zhang, Chunxiao Liu, Liu Yang, Tong Ji, Nana Zhang, Xiaoqing Dong, Xin Chen, Jing Ma, Wei Gao, Shile Huang, Long Chen
Summary: Research shows that PD toxins induce neuronal apoptosis by generating H2O2 through NOX2, hindering the AMPK/Akt-mTOR signaling pathway. Inhibiting NOX2 or using antioxidants may help alleviate neuronal loss caused by oxidative stress.
CELLULAR SIGNALLING
(2022)
Article
Integrative & Complementary Medicine
Yu Gan, Shi-Le Huang, Meng-Qi Luo, Min Chen, Hui Zheng
Summary: The efficacy of acupuncture combined with usual care in treating irritable bowel syndrome (IBS) has been studied through network meta-analysis. The results show that manual acupuncture combined with usual care is the most effective approach, and adding manual acupuncture to usual care provides additional benefits.
ACUPUNCTURE IN MEDICINE
(2022)
Review
Pharmacology & Pharmacy
Di Qin, Qing-Feng Tao, Shi-Le Huang, Min Chen, Hui Zheng
Summary: This study compared the efficacy of eluxadoline and antispasmodics in the treatment of irritable bowel syndrome (IBS). The results showed that eluxadoline was as effective as antispasmodics in relieving abdominal pain, but had more reported adverse events. Therefore, antispasmodics remain the first choice for the treatment of IBS.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Pharmacology & Pharmacy
Qinghui Lan, Peiqing Guan, Chunzheng Huang, Shile Huang, Peiling Zhou, Changzheng Zhang
Summary: This study demonstrated that arecoline significantly increased the firing rate of dopaminergic neurons and induced burst firing. This may contribute to areca nut dependency and provide a potential target for cessation therapy.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Immunology
Tithi Roy, Sergette Banang-Mbeumi, Samuel T. Boateng, Emmanuelle M. Ruiz, Roxane-Cherille N. Chamcheu, Lin Kang, Judy A. King, Anthony L. Walker, Bolni Marius Nagalo, Konstantin G. Kousoulas, Stephane Esnault, Shile Huang, Jean Christopher Chamcheu
Summary: Fisetin has potential therapeutic effects on refractory psoriasis by inhibiting abnormal immune response, promoting keratinocyte differentiation and autophagy, and reducing disease inflammation.
FRONTIERS IN IMMUNOLOGY
(2023)
Correction
Neurosciences
Qianyun Yu, Ruijie Zhang, Tianjing Li, Liu Yang, Zhihan Zhou, Long Hou, Wen Wu, Rui Zhao, Xiaoling Chen, Yajie Yao, Shile Huang, Long Chen
MOLECULAR NEUROBIOLOGY
(2023)
Article
Neurosciences
Qianyun Yu, Ruijie Zhang, Tianjing Li, Liu Yang, Zhihan Zhou, Long Hou, Wen Wu, Rui Zhao, Xiaoling Chen, Yajie Yao, Shile Huang, Long Chen
Summary: Defective autophagy is involved in the development of Parkinson's disease (PD), and PD toxins impede autophagy through the PTEN/Akt signaling pathway, leading to neuronal apoptosis. Activation of PTEN and inactivation of Akt by PD toxins inhibit autophagy, and this process is regulated by the protein Parkin. Manipulation of PTEN/Akt/autophagy signaling with antioxidants could be a potential therapeutic strategy for preventing neuronal loss in PD.
MOLECULAR NEUROBIOLOGY
(2023)
Article
Biology
Terry C. Lairmore, Jehan Abdulsattar, Arrigo De Benedetti, Runhua Shi, Shile Huang, Md Imtiaz Khalil, Stephan N. Witt
Summary: Immunohistochemical analysis showed an inverse association between menin expression and tumor grade in cholangiocarcinoma. Menin expression was higher in low-grade tumors and lower in high-grade tumors. Menin expression was also positively associated with fibrosis status.
BMC RESEARCH NOTES
(2023)
Article
Biochemistry & Molecular Biology
Boyue Zhang, Ting Cai, Hongling He, Xuezhe Huang, Guie Chen, Yanqin Lai, Yongwen Luo, Shile Huang, Jun Luo, Xiaofeng Guo
Summary: Rabies is a highly fatal zoonotic disease with no effective treatment available. Recent studies have shown that the tripartite-motif protein (TRIM) family plays a role in regulating viral replication. Specifically, tripartite-motif protein 21 (TRIM21) has been found to be upregulated in the brain tissue of mice infected with rabies virus and its knockdown inhibits viral replication. Knockdown of interferon-alpha and interferon-beta further modulates the inhibition of viral replication caused by TRIM21 knockdown. TRIM21 regulates RABV replication via the IRF7-IFN axis, highlighting its potential as a target for rabies treatment or management.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Shasha Liu, Siya Liu, Ziding Yu, Wenzhuo Zhou, Meichun Zheng, Rongrong Gu, Jinxuan Hong, Zhou Yang, Xiaojuan Chi, Guijie Guo, Xinxin Li, Na Chen, Shile Huang, Song Wang, Ji-Long Chen
Summary: This study reveals that early-stage infection with various viruses, including influenza A virus, leads to IL-6-independent phosphorylation of STAT3 Y705, which is critical for antiviral immunity. Knockin mice with STAT3Y705F/1 mutation show suppressed antiviral response, lung tissue injury, and poor survival upon IAV infection. The phosphorylation of STAT3 Y705 restrains IAV pathogenesis by repressing excessive IFN production, and blocking the phosphorylation enhances IFN expression and potentiates IAV virulence in mice. Knockout of IFNAR1 or IFNLR1 in STAT3Y705F/1 mice protects them from lung injury and reduces viral load.
Article
Genetics & Heredity
Boyue Zhang, Ting Cai, Hongling He, Xuezhe Huang, Yongwen Luo, Shile Huang, Jun Luo, Xiaofeng Guo
Summary: TRIM25 plays a crucial role in the infection of rabies virus, regulating its replication through the RIG-I-IFN axis. This finding suggests that TRIM25 may serve as a potential target for the development of antiviral drugs against rabies.
Meeting Abstract
Oncology
Lin Li, Lie Liu, Shile Huang
Meeting Abstract
Oncology
Shile Huang, Lei Liu, Yan Luo
