3D Primary Hepatocyte Culture Systems for Analyses of Liver Diseases, Drug Metabolism, and Toxicity: Emerging Culture Paradigms and Applications

Bioprinted liver provides early insight into the role of Kupffer cells in TGF-β1 and methotrexate-induced fibrogenesis

(2019) Leah M. Norona et al.   PLoS One

3D bioprinting of functional tissue models for personalized drug screening and in vitro disease modeling

(2018) Xuanyi Ma et al.   ADVANCED DRUG DELIVERY REVIEWS

Application of Microphysiological Systems to Enhance Safety Assessment in Drug Discovery

(2018) Lorna Ewart et al.   Annual Review of Pharmacology and Toxicology

Physiologically Based Pharmacokinetic and Pharmacodynamic Analysis Enabled by Microfluidically Linked Organs-on-Chips

(2018) Rachelle Prantil-Baun et al.   Annual Review of Pharmacology and Toxicology

Organs-on-a-Chip: A Fast Track for Engineered Human Tissues in Drug Development

(2018) Kacey Ronaldson-Bouchard et al.   Cell Stem Cell

Translating scientific discovery: the need for preclinical models of nonalcoholic steatohepatitis

(2018) Abdul M. Oseini et al.   Hepatology International

Human-based systems: Mechanistic NASH modelling just around the corner?

(2018) Joost Boeckmans et al.   PHARMACOLOGICAL RESEARCH

Prediction of Drug-Induced Hepatotoxicity Using Long-Term Stable Primary Hepatic 3D Spheroid Cultures in Chemically Defined Conditions

(2018) Sabine U Vorrink et al.   TOXICOLOGICAL SCIENCES

Comparison of Hepatic 2D Sandwich Cultures and 3D Spheroids for Long-term Toxicity Applications: A Multicenter Study

(2018) Catherine C Bell et al.   TOXICOLOGICAL SCIENCES

Perfused human hepatocyte microtissues identify reactive metabolite-forming and mitochondria-perturbing hepatotoxins

(2018) Cliff Rowe et al.   TOXICOLOGY IN VITRO

Inter-individual differences in the susceptibility of primary human hepatocytes towards drug-induced cholestasis are compound and time dependent

(2018) Céline Parmentier et al.   TOXICOLOGY LETTERS

3D microfluidic liver cultures as a physiological preclinical tool for hepatitis B virus infection

(2018) A. M. Ortega-Prieto et al.   Nature Communications

HLA-A*33:03-Restricted Activation of Ticlopidine-Specific T-Cells from Human Donors

(2018) Toru Usui et al.   CHEMICAL RESEARCH IN TOXICOLOGY

Modeling Host-Pathogen Interactions in the Context of the Microenvironment: 3-D Cell Culture Comes of Age

(2018) Jennifer Barrila et al.   INFECTION AND IMMUNITY

A glass-based, continuously zonated and vascularized human liver acinus microphysiological system (vLAMPS) designed for experimental modeling of diseases and ADME/TOX

(2018) Xiang Li et al.   LAB ON A CHIP

Human liver spheroids in chemically defined conditions for studies of gene–drug, drug–drug and disease–drug interactions

(2018) Magnus Ingelman-Sundberg et al.   PHARMACOGENOMICS

Three-Dimensional Spheroid Primary Human Hepatocytes in Monoculture and Coculture with Nonparenchymal Cells

(2018) Audrey Baze et al.   TISSUE ENGINEERING PART C-METHODS

Human hepatic 3D spheroids as a model for steatosis and insulin resistance

(2018) Mikael Kozyra et al.   Scientific Reports

AMP-activated protein kinase activation and NADPH oxidase inhibition by inorganic nitrate and nitrite prevent liver steatosis

(2018) Isabel Cordero-Herrera et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Control of oxygen tension recapitulates zone-specific functions in human liver microphysiology systems

(2017) Felipe T Lee-Montiel et al.   EXPERIMENTAL BIOLOGY AND MEDICINE

Endogenous and xenobiotic metabolic stability of primary human hepatocytes in long-term 3D spheroid cultures revealed by a combination of targeted and untargeted metabolomics

(2017) Sabine U. Vorrink et al.   FASEB JOURNAL

Microengineered cultures containing human hepatic stellate cells and hepatocytes for drug development

(2017) Matthew D. Davidson et al.   Integrative Biology

Mimicking liver sinusoidal structures and functions using a 3D-configured microfluidic chip

(2017) Yu Du et al.   LAB ON A CHIP

miR-122 Release in Exosomes Precedes Overt Tolvaptan-Induced Necrosis in a Primary Human Hepatocyte Micropatterned Coculture Model

(2017) Merrie Mosedale et al.   TOXICOLOGICAL SCIENCES

Exploring Chronic Drug Effects on Microengineered Human Liver Cultures Using Global Gene Expression Profiling

(2017) Brenton R. Ware et al.   TOXICOLOGICAL SCIENCES

Three-dimensional perfused human in vitro model of non-alcoholic fatty liver disease

(2017) Tomasz Kostrzewski et al.   WORLD JOURNAL OF GASTROENTEROLOGY

Biophysical Regulation of Cell Behavior—Cross Talk between Substrate Stiffness and Nanotopography

(2017) Yong Yang et al.   Engineering

Validation of precision-cut liver slices to study drug-induced cholestasis: a transcriptomics approach

(2016) Suresh Vatakuti et al.   ARCHIVES OF TOXICOLOGY

A multicenter assessment of single-cell models aligned to standard measures of cell health for prediction of acute hepatotoxicity

(2016) Rowena L. Sison-Young et al.   ARCHIVES OF TOXICOLOGY

Novel 3D Culture Systems for Studies of Human Liver Function and Assessments of the Hepatotoxicity of Drugs and Drug Candidates

(2016) Volker M. Lauschke et al.   CHEMICAL RESEARCH IN TOXICOLOGY

DILIrank: the largest reference drug list ranked by the risk for developing drug-induced liver injury in humans

(2016) Minjun Chen et al.   DRUG DISCOVERY TODAY

The economic and clinical burden of nonalcoholic fatty liver disease in the United States and Europe

(2016) Zobair M. Younossi et al.   HEPATOLOGY

Global epidemiology of nonalcoholic fatty liver disease-Meta-analytic assessment of prevalence, incidence, and outcomes

(2016) Zobair M. Younossi et al.   HEPATOLOGY

Massive rearrangements of cellular MicroRNA signatures are key drivers of hepatocyte dedifferentiation

(2016) Volker M. Lauschke et al.   HEPATOLOGY

The Importance of Patient-Specific Factors for Hepatic Drug Response and Toxicity

(2016) Volker Lauschke et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Bioprinting towards Physiologically Relevant Tissue Models for Pharmaceutics

(2016) Weijie Peng et al.   TRENDS IN BIOTECHNOLOGY

Long-term exposure to abnormal glucose levels alters drug metabolism pathways and insulin sensitivity in primary human hepatocytes

(2016) Matthew D. Davidson et al.   Scientific Reports

Integrated Genomic Analysis of Diverse Induced Pluripotent Stem Cells from the Progenitor Cell Biology Consortium

(2016) Nathan Salomonis et al.   Stem Cell Reports

3D Printed Tissue Models: Present and Future

(2016) Jinah Jang et al.   ACS Biomaterials-Science & Engineering

3D Printed Tissue Models: Present and Future

(2016) Jinah Jang et al.   ACS Biomaterials Science & Engineering

Bioprinted 3D Primary Liver Tissues Allow Assessment of Organ-Level Response to Clinical Drug Induced Toxicity In Vitro

(2016) Deborah G. Nguyen et al.   PLoS One

Characterization of primary human hepatocyte spheroids as a model system for drug-induced liver injury, liver function and disease

(2016) Catherine C. Bell et al.   Scientific Reports

Hepatic 3D spheroid models for the detection and study of compounds with cholestatic liability

(2016) Delilah F. G. Hendriks et al.   Scientific Reports

A hydrogel bioink toolkit for mimicking native tissue biochemical and mechanical properties in bioprinted tissue constructs

(2015) Aleksander Skardal et al.   Acta Biomaterialia

A human liver microphysiology platform for investigating physiology, drug safety, and disease models

(2015) Lawrence A Vernetti et al.   EXPERIMENTAL BIOLOGY AND MEDICINE

Characterization of amoxicillin- and clavulanic acid-specific T cells in patients with amoxicillin-clavulanate-induced liver injury

(2015) Seung-Hyun Kim et al.   HEPATOLOGY

Phenotypic and functional analyses show stem cell-derived hepatocyte-like cells better mimic fetal rather than adult hepatocytes

(2015) Melissa Baxter et al.   JOURNAL OF HEPATOLOGY

Reaction injection molding and direct covalent bonding of OSTE+ polymer microfluidic devices

(2015) N Sandström et al.   JOURNAL OF MICROMECHANICS AND MICROENGINEERING

Three-dimensional co-culture of human hepatocytes and mesenchymal stem cells: improved functionality in long-term bioreactor cultures

(2015) Sofia P. Rebelo et al.   Journal of Tissue Engineering and Regenerative Medicine

Prediction of Drug-Induced Liver Injury in Micropatterned Co-cultures Containing iPSC-Derived Human Hepatocytes

(2015) Brenton R. Ware et al.   TOXICOLOGICAL SCIENCES

Enhanced Metabolizing Activity of Human ES Cell-Derived Hepatocytes Using a 3D Culture System with Repeated Exposures to Xenobiotics

(2015) Jong Hyun Kim et al.   TOXICOLOGICAL SCIENCES

Comparative Proteomic Characterization of 4 Human Liver-Derived Single Cell Culture Models Reveals Significant Variation in the Capacity for Drug Disposition, Bioactivation, and Detoxication

(2015) Rowena L. C. Sison-Young et al.   TOXICOLOGICAL SCIENCES

Pluripotent stem cell-derived hepatocyte-like cells

(2014) R.E. Schwartz et al.   BIOTECHNOLOGY ADVANCES

Evaluation of Human Hepatocytes Cultured by Three-dimensional Spheroid Systems for Drug Metabolism

(2014) Takako Ohkura et al.   Drug Metabolism and Pharmacokinetics

In vitro platforms for evaluating liver toxicity

(2014) Shyam Sundhar Bale et al.   EXPERIMENTAL BIOLOGY AND MEDICINE

Functional off-stoichiometry thiol-ene-epoxy thermosets featuring temporally controlled curing stages via an UV/UV dual cure process

(2014) Carl Fredrik Carlborg et al.   JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY

Dynamic interplay of flow and collagen stabilizes primary hepatocytes culture in a microfluidic platform

(2014) Manjunath Hegde et al.   LAB ON A CHIP

Drug-Induced Liver Injury

(2014) Michael D. Leise et al.   MAYO CLINIC PROCEEDINGS

Lessons learned from the fate of AstraZeneca's drug pipeline: a five-dimensional framework

(2014) David Cook et al.   NATURE REVIEWS DRUG DISCOVERY

Maturation of Induced Pluripotent Stem Cell Derived Hepatocytes by 3D-Culture

(2014) Richard L. Gieseck III et al.   PLoS One

The 3D printing of gelatin methacrylamide cell-laden tissue-engineered constructs with high cell viability

(2013) Thomas Billiet et al.   BIOMATERIALS

A Microscale Human Liver Platform that Supports the Hepatic Stages of Plasmodium falciparum and vivax

(2013) Sandra March et al.   Cell Host & Microbe

Developing High-Fidelity Hepatotoxicity Models From Pluripotent Stem Cells

(2013) Claire N. Medine et al.   Stem Cells Translational Medicine

Multi-cell type human liver microtissues for hepatotoxicity testing

(2012) S. Messner et al.   ARCHIVES OF TOXICOLOGY

3D spheroid culture of hESC/hiPSC-derived hepatocyte-like cells for drug toxicity testing

(2012) Kazuo Takayama et al.   BIOMATERIALS

Preclinical Strategy to Reduce Clinical Hepatotoxicity Using in Vitro Bioactivation Data for >200 Compounds

(2012) Melanie Z. Sakatis et al.   CHEMICAL RESEARCH IN TOXICOLOGY

Human leukocyte antigen (HLA)-B*57:01-restricted activation of drug-specific T cells provides the immunological basis for flucloxacillin-induced liver injury

(2012) Manal M. Monshi et al.   HEPATOLOGY

Use of Micropatterned Cocultures to Detect Compounds That Cause Drug-Induced Liver Injury in Humans

(2012) Salman R. Khetani et al.   TOXICOLOGICAL SCIENCES

In vitro models for liver toxicity testing

(2012) Valerie Y. Soldatow et al.   Toxicology Research

Human liver cell spheroids in extended perfusion bioreactor culture for repeated-dose drug testing

(2011) Rui M. Tostões et al.   HEPATOLOGY

Assessment of a Micropatterned Hepatocyte Coculture System to Generate Major Human Excretory and Circulating Drug Metabolites

(2010) W. W. Wang et al.   DRUG METABOLISM AND DISPOSITION

A Comparison of Whole Genome Gene Expression Profiles of HepaRG Cells and HepG2 Cells to Primary Human Hepatocytes and Human Liver Tissues

(2010) S. N. Hart et al.   DRUG METABOLISM AND DISPOSITION

Sandwich-cultured hepatocytes: anin vitromodel to evaluate hepatobiliary transporter-based drug interactions and hepatotoxicity

(2010) Brandon Swift* et al.   DRUG METABOLISM REVIEWS

Photocrosslinkable Hyaluronan-Gelatin Hydrogels for Two-Step Bioprinting

(2010) Aleksander Skardal et al.   TISSUE ENGINEERING PART A

HepaRG human hepatic cell line utility as a surrogate for primary human hepatocytes in drug metabolism assessment in vitro

(2010) Marc Lübberstedt et al.   JOURNAL OF PHARMACOLOGICAL AND TOXICOLOGICAL METHODS

Predicting Circulating Human Metabolites: How Good Are We?

(2009) Shelby Anderson et al.   CHEMICAL RESEARCH IN TOXICOLOGY

Assessment of Three Human in Vitro Systems in the Generation of Major Human Excretory and Circulating Metabolites

(2009) Deepak Dalvie et al.   CHEMICAL RESEARCH IN TOXICOLOGY

Biological implications of polydimethylsiloxane-based microfluidic cell culture

(2009) Keil J. Regehr et al.   LAB ON A CHIP

Perfused multiwell plate for 3D liver tissue engineering

(2009) Karel Domansky et al.   LAB ON A CHIP

T-cadherin modulates hepatocyte functions in vitro

(2008) Salman R. Khetani et al.   FASEB JOURNAL

Bioactive Contaminants Leach from Disposable Laboratory Plasticware

(2008) G. R. McDonald et al.   SCIENCE

Cellular Imaging Predictions of Clinical Drug-Induced Liver Injury

(2008) Jinghai J. Xu et al.   TOXICOLOGICAL SCIENCES