Liver organoid as a 3D in vitro model for drug validation and toxicity assessment

Development of a three‐dimensional in vitro co‐culture model to increase drug selectivity for humans

(2020) Sung Bum Park et al.   DIABETES OBESITY & METABOLISM

LGR5 marks targetable tumor-initiating cells in mouse liver cancer

(2020) Wanlu Cao et al.   Nature Communications

CDKN2a/p16 Antagonizes Hepatic Stellate Cell Activation and Liver Fibrosis by Modulating ROS Levels

(2020) Fangqiao Lv et al.   Frontiers in Cell and Developmental Biology

Human Pluripotent Stem Cell-Derived Organoids as Models of Liver Disease

(2020) Muhammad Nadzim Bin Ramli et al.   GASTROENTEROLOGY

High-Fidelity Drug Induced Liver Injury Screen Using Human PSC-derived Organoids

(2020) Tadahiro Shinozawa et al.   GASTROENTEROLOGY

Homeostasis of Glucose and Lipid in Non-Alcoholic Fatty Liver Disease

(2019) Hsu-Wen Chao et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Remodelling and Improvements in Organoid Technology to Study Liver Carcinogenesis in a Dish

(2019) Umesh Tharehalli et al.   Stem Cells International

Lgr5+ stem/progenitor cells reside at the apex of a heterogeneous embryonic hepatoblast pool

(2019) Nicole Prior et al.   DEVELOPMENT

Establishing Pure Cancer Organoid Cultures: Identification, Selection and Verification of Cancer Phenotypes and Genotypes

(2019) Nina Wallaschek et al.   JOURNAL OF MOLECULAR BIOLOGY

Human organoids: a new dimension in cell biology

(2019) Ruth Lehmann et al.   MOLECULAR BIOLOGY OF THE CELL

Organoids of liver diseases: From bench to bedside

(2019) Li-Jun Wu et al.   WORLD JOURNAL OF GASTROENTEROLOGY

Modeling Steatohepatitis in Humans with Pluripotent Stem Cell-Derived Organoids

(2019) Rie Ouchi et al.   Cell Metabolism

Liver organoids: from basic research to therapeutic applications

(2019) Nicole Prior et al.   GUT

Generation of expandable human pluripotent stem cell-derived hepatocyte-like liver organoids

(2019) Seon Ju Mun et al.   JOURNAL OF HEPATOLOGY

Human ESC-derived expandable hepatic organoids enable therapeutic liver repopulation and pathophysiological modeling of alcoholic liver injury

(2019) Shuyong Wang et al.   CELL RESEARCH

Large‐scale Production of LGR5‐positive Bipotential Human Liver Stem Cells

(2019) Kerstin Schneeberger et al.   HEPATOLOGY

Remote Actuation of Apoptosis in Liver Cancer Cells via Magneto-Mechanical Modulation of Iron Oxide Nanoparticles

(2019) Oleg Lunov et al.   Cancers

Recent advances in alcohol-related liver disease (ALD): summary of a Gut round table meeting

(2019) Matias A Avila et al.   GUT

Liver organoids reproduce alpha-1 antitrypsin deficiency-related liver disease

(2019) Gema Gómez-Mariano et al.   Hepatology International

Drug-Induced Liver Injury: Biomarkers, Requirements, Candidates, and Validation

(2019) Lucy Meunier et al.   Frontiers in Pharmacology

Next-Generation Liver Medicine Using Organoid Models

(2019) Soheil Akbari et al.   Frontiers in Cell and Developmental Biology

Functional 3D Human Liver Bud Assembled from MSC-Derived Multiple Liver Cell Lineages

(2018) Jing Li et al.   CELL TRANSPLANTATION

Modeling human diseases with induced pluripotent stem cells: from 2D to 3D and beyond

(2018) Chun Liu et al.   DEVELOPMENT

Self-assembled liver organoids recapitulate hepatobiliary organogenesis in vitro

(2018) Dipen Vyas et al.   HEPATOLOGY

Three-dimensional liver-derived extracellular matrix hydrogel promotes liver organoids function

(2018) Mona Saheli et al.   JOURNAL OF CELLULAR BIOCHEMISTRY

Three-Dimensional in Vitro Cell Culture Models in Drug Discovery and Drug Repositioning

(2018) Sigrid A. Langhans   Frontiers in Pharmacology

Liver diseases in the dish: iPSC and organoids as a new approach to modeling liver diseases

(2018) Romina Fiorotto et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE

miR-122 inhibition in a human liver organoid model leads to liver inflammation, necrosis, steatofibrosis and dysregulated insulin signaling

(2018) Hossein Sendi et al.   PLoS One

Organoid Models of Human Liver Cancers Derived from Tumor Needle Biopsies

(2018) Sandro Nuciforo et al.   Cell Reports

Recapitulation of hepatitis B virus–host interactions in liver organoids from human induced pluripotent stem cells

(2018) Yun-Zhong Nie et al.   EBioMedicine

Retinoids Issued from Hepatic Stellate Cell Lipid Droplet Loss as Potential Signaling Molecules Orchestrating a Multicellular Liver Injury Response

(2018) Marie Bobowski-Gerard et al.   Cells

Long-Term Expansion of Functional Mouse and Human Hepatocytes as 3D Organoids

(2018) Huili Hu et al.   CELL

Functional properties of hepatocytes in vitro are correlated with cell polarity maintenance

(2017) Anja Zeigerer et al.   EXPERIMENTAL CELL RESEARCH

Dynamics of Proliferative and Quiescent Stem Cells in Liver Homeostasis and Injury

(2017) Wanlu Cao et al.   GASTROENTEROLOGY

Advances in multicellular spheroids formation

(2017) X. Cui et al.   Journal of the Royal Society Interface

Human primary liver cancer–derived organoid cultures for disease modeling and drug screening

(2017) Laura Broutier et al.   NATURE MEDICINE

Immunohistochemical Analysis of the Stem Cell Marker LGR5 in Pediatric Liver Disease

(2017) Zahida Khan et al.   PEDIATRIC AND DEVELOPMENTAL PATHOLOGY

Modeling Physiological Events in 2D vs. 3D Cell Culture

(2017) Kayla Duval et al.   PHYSIOLOGY

A Scalable Approach for the Generation of Human Pluripotent Stem Cell-Derived Hepatic Organoids with Sensitive Hepatotoxicity Features

(2017) Malte Sgodda et al.   STEM CELLS AND DEVELOPMENT

From the Cover: Three-Dimensional (3D) HepaRG Spheroid Model With Physiologically Relevant Xenobiotic Metabolism Competence and Hepatocyte Functionality for Liver Toxicity Screening

(2017) Sreenivasa C. Ramaiahgari et al.   TOXICOLOGICAL SCIENCES

Novel human hepatic organoid model enables testing of drug-induced liver fibrosis in vitro

(2016) Sofia B. Leite et al.   BIOMATERIALS

Cell sources for in vitro human liver cell culture models

(2016) Katrin Zeilinger et al.   EXPERIMENTAL BIOLOGY AND MEDICINE

Three-dimensional models for studying development and disease: moving on from organisms to organs-on-a-chip and organoids

(2016) E. L. Jackson et al.   Integrative Biology

Organoids: A historical perspective of thinking in three dimensions

(2016) Marina Simian et al.   JOURNAL OF CELL BIOLOGY

Concise Review: Organoids Are a Powerful Tool for the Study of Liver Disease and Personalized Treatment Design in Humans and Animals

(2016) Sathidpak Nantasanti et al.   Stem Cells Translational Medicine

Exosomes from Human Umbilical Cord Mesenchymal Stem Cells: Identification, Purification, and Biological Characteristics

(2016) Bin Zhang et al.   Stem Cells International

Mechanisms and therapeutic effectiveness of pulsed electromagnetic field therapy in oncology

(2016) Maria Vadalà et al.   Cancer Medicine

Toxicity testing: the search for an in vitro alternative to animal testing

(2016) J. E. May et al.   BRITISH JOURNAL OF BIOMEDICAL SCIENCE

Engineered In Vitro Disease Models

(2015) Kambez H. Benam et al.   Annual Review of Pathology-Mechanisms of Disease

Long-Term Culture of Genome-Stable Bipotent Stem Cells from Adult Human Liver

(2015) Meritxell Huch et al.   CELL

Modeling mouse and human development using organoid cultures

(2015) M. Huch et al.   DEVELOPMENT

Directed differentiation of cholangiocytes from human pluripotent stem cells

(2015) Mina Ogawa et al.   NATURE BIOTECHNOLOGY

Cholangiocytes derived from human induced pluripotent stem cells for disease modeling and drug validation

(2015) Fotios Sampaziotis et al.   NATURE BIOTECHNOLOGY

High-yield and high-purity isolation of hepatic stellate cells from normal and fibrotic mouse livers

(2015) Ingmar Mederacke et al.   Nature Protocols

Prediction of Drug-Induced Liver Injury in Micropatterned Co-cultures Containing iPSC-Derived Human Hepatocytes

(2015) Brenton R. Ware et al.   TOXICOLOGICAL SCIENCES

In Vitro Generation of Functional Liver Organoid-Like Structures Using Adult Human Cells

(2015) Sarada Devi Ramachandran et al.   PLoS One

Three-Dimensional Cell Culture Systems and Their Applications in Drug Discovery and Cell-Based Biosensors

(2014) Rasheena Edmondson et al.   ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES

In vitro platforms for evaluating liver toxicity

(2014) Shyam Sundhar Bale et al.   EXPERIMENTAL BIOLOGY AND MEDICINE

Generation of functional cholangiocyte-like cells from human pluripotent stem cells and HepaRG cells

(2014) Noushin Dianat et al.   HEPATOLOGY

Strategies for immortalization of primary hepatocytes

(2014) Eva Ramboer et al.   JOURNAL OF HEPATOLOGY

Hepatic organoids for microfluidic drug screening

(2014) Sam H. Au et al.   LAB ON A CHIP

Hematopoietic stem cell niche maintenance during homeostasis and regeneration

(2014) Avital Mendelson et al.   NATURE MEDICINE

In vitro expansion of single Lgr5+ liver stem cells induced by Wnt-driven regeneration

(2013) Meritxell Huch et al.   NATURE

Vascularized and functional human liver from an iPSC-derived organ bud transplant

(2013) Takanori Takebe et al.   NATURE

Three-dimensional cell culture: the missing link in drug discovery

(2012) Susan Breslin et al.   DRUG DISCOVERY TODAY

Three-Dimensional HepaRG Model As An Attractive Tool for Toxicity Testing

(2012) Sofia B. Leite et al.   TOXICOLOGICAL SCIENCES

Mechanisms of Cell Death in Acute Liver Failure

(2012) Heike Bantel et al.   Frontiers in Physiology

A novel 3D liver organoid system for elucidation of hepatic glucose metabolism

(2011) Yanhua Lu et al.   BIOTECHNOLOGY AND BIOENGINEERING

Results of a phase IIa study of VX-809, an investigational CFTR corrector compound, in subjects with cystic fibrosis homozygous for theF508del-CFTRmutation

(2011) J P Clancy et al.   THORAX

Reducing Polycystic Liver Volume in ADPKD: Effects of Somatostatin Analogue Octreotide

(2010) A. Caroli et al.   Clinical Journal of the American Society of Nephrology

Drug-Induced Idiosyncratic Hepatotoxicity: Prevention Strategy Developed after the Troglitazone Case

(2010) Toshihiko Ikeda   Drug Metabolism and Pharmacokinetics

Genetic Labeling Does Not Detect Epithelial-to-Mesenchymal Transition of Cholangiocytes in Liver Fibrosis in Mice

(2010) David Scholten et al.   GASTROENTEROLOGY

Prediction of Hepatic Clearance in Human From In Vitro Data for Successful Drug Development

(2009) Masato Chiba et al.   AAPS Journal

Current Concepts of Mechanisms in Drug-Induced Hepatotoxicity

(2009) Stefan Russmann et al.   CURRENT MEDICINAL CHEMISTRY

Single nucleotide polymorphism-mediated translational suppression of endoplasmic reticulum mannosidase I modifies the onset of end-stage liver disease in alpha1-antitrypsin deficiency

(2009) Shujuan Pan et al.   HEPATOLOGY

Intrinsic versus Idiosyncratic Drug-Induced Hepatotoxicity--Two Villains or One?

(2009) R. A. Roth et al.   JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS