Modeling Physiological Events in 2D vs. 3D Cell Culture

3D culture for cardiac cells

(2016) Christian Zuppinger   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH

Microarray analyses to quantify advantages of 2D and 3D hydrogel culture systems in maintaining the native valvular interstitial cell phenotype

(2016) Kelly M. Mabry et al.   BIOMATERIALS

Winner of the Young Investigator Award of the Society for Biomaterials at the 10th World Biomaterials Congress, May 17-22, 2016, Montreal QC, Canada: Microribbon-based hydrogels accelerate stem cell-based bone regeneration in a mouse critical-size cranial

(2016) Li-Hsin Han et al.   JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A

Hidden in the mist no more: physical force in cell biology

(2016) Karin Wang et al.   NATURE METHODS

Spheroid Mesenchymal Stem Cells and Mesenchymal Stem Cell-Derived Microvesicles: Two Potential Therapeutic Strategies

(2016) Lili Xie et al.   STEM CELLS AND DEVELOPMENT

Spheroid Culture of Mesenchymal Stem Cells

(2016) Zoe Cesarz et al.   Stem Cells International

Development of an Automated and Sensitive Microfluidic Device for Capturing and Characterizing Circulating Tumor Cells (CTCs) from Clinical Blood Samples

(2016) Priya Gogoi et al.   PLoS One

Agarose particle-templated porous bacterial cellulose and its application in cartilage growth in vitro

(2015) Na Yin et al.   Acta Biomaterialia

High-Content Assays for Characterizing the Viability and Morphology of 3D Cancer Spheroid Cultures

(2015) Oksana Sirenko et al.   ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES

Vascularization of hollow channel-modified porous silk scaffolds with endothelial cells for tissue regeneration

(2015) Wenjie Zhang et al.   BIOMATERIALS

Differential basal-to-apical accessibility of lamin A/C epitopes in the nuclear lamina regulated by changes in cytoskeletal tension

(2015) Teemu O. Ihalainen et al.   NATURE MATERIALS

Accelerated wound healing by injectable microporous gel scaffolds assembled from annealed building blocks

(2015) Donald R. Griffin et al.   NATURE MATERIALS

Hydrogels with tunable stress relaxation regulate stem cell fate and activity

(2015) Ovijit Chaudhuri et al.   NATURE MATERIALS

Three-dimensional force microscopy of cells in biopolymer networks

(2015) Julian Steinwachs et al.   NATURE METHODS

Organs-on-chips at the frontiers of drug discovery

(2015) Eric W. Esch et al.   NATURE REVIEWS DRUG DISCOVERY

Comparison of 2D- and 3D-culture models as drug-testing platforms in breast cancer

(2015) YOSHINORI IMAMURA et al.   ONCOLOGY REPORTS

The Tissue-Engineered Vascular Graft—Past, Present, and Future

(2015) Samand Pashneh-Tala et al.   TISSUE ENGINEERING PART B-REVIEWS

Development and Characterization of a Scaffold-Free 3D Spheroid Model of Induced Pluripotent Stem Cell-Derived Human Cardiomyocytes

(2015) Philippe Beauchamp et al.   TISSUE ENGINEERING PART C-METHODS

Cell viability assessment using the Alamar blue assay: A comparison of 2D and 3D cell culture models

(2015) F. Bonnier et al.   TOXICOLOGY IN VITRO

Dynamic tensile forces drive collective cell migration through three-dimensional extracellular matrices

(2015) Nikolce Gjorevski et al.   Scientific Reports

A Cancer Cell Spheroid Assay to Assess Invasion in a 3D Setting

(2015) Eric B. Berens et al.   Jove-Journal of Visualized Experiments

Three-Dimensional Cell Culture Systems and Their Applications in Drug Discovery and Cell-Based Biosensors

(2014) Rasheena Edmondson et al.   ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES

Optimization of Aqueous Biphasic Tumor Spheroid Microtechnology for Anti-cancer Drug Testing in 3D Culture

(2014) Stephanie Lemmo et al.   Cellular and Molecular Bioengineering

Engineering of arteries in vitro

(2014) Angela H. Huang et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

Forms, forces, and stem cell fate

(2014) Evangelia Bellas et al.   CURRENT OPINION IN CELL BIOLOGY

Clinical Application of a Tissue-Cultured Skin Autograft: An Alternative for the Treatment of Non-Healing or Slowly Healing Wounds?

(2014) Nadja Zöller et al.   DERMATOLOGY

The present and future role of microfluidics in biomedical research

(2014) Eric K. Sackmann et al.   NATURE

Microfluidic organs-on-chips

(2014) Sangeeta N Bhatia et al.   NATURE BIOTECHNOLOGY

Advances in skin grafting and treatment of cutaneous wounds

(2014) B. K. Sun et al.   SCIENCE

Chondrogenic Differentiation of Adipose-Derived Stromal Cells in Combinatorial Hydrogels Containing Cartilage Matrix Proteins with Decoupled Mechanical Stiffness

(2014) Tianyi Wang et al.   TISSUE ENGINEERING PART A

Cardiogenesis of Embryonic Stem Cells with Liquid Marble Micro-Bioreactor

(2014) Fatemeh Sarvi et al.   Advanced Healthcare Materials

Controlling Major Cellular Processes of Human Mesenchymal Stem Cells using Microwell Structures

(2014) Xun Xu et al.   Advanced Healthcare Materials

Roles of Spheroid Formation of Hepatocytes in Liver Tissue Engineering

(2014) Hu-Lin Jiang et al.   International Journal of Stem Cells

Photo-crosslinkable PEG-Based Microribbons for Forming 3D Macroporous Scaffolds with Decoupled Niche Properties

(2013) Li-Hsin Han et al.   ADVANCED MATERIALS

Dynamic tissue engineering scaffolds with stimuli-responsive macroporosity formation

(2013) Li-Hsin Han et al.   BIOMATERIALS

Differentiation Patterns of Embryonic Stem Cells in Two- versus Three-Dimensional Culture

(2013) Emma T. Pineda et al.   CELLS TISSUES ORGANS

Tissue, cell and engineering

(2013) Kyongbum Lee et al.   CURRENT OPINION IN BIOTECHNOLOGY

Spheroid-based three-dimensional liver-on-a-chip to investigate hepatocyte–hepatic stellate cell interactions and flow effects

(2013) Seung-A Lee et al.   LAB ON A CHIP

Microfabrication of human organs-on-chips

(2013) Dongeun Huh et al.   Nature Protocols

Spheroid culture as a tool for creating 3D complex tissues

(2013) Eelco Fennema et al.   TRENDS IN BIOTECHNOLOGY

The effects of interactive mechanical and biochemical niche signaling on osteogenic differentiation of adipose-derived stem cells using combinatorial hydrogels

(2012) Michelle Nii et al.   Acta Biomaterialia

Microribbon-Like Elastomers for Fabricating Macroporous and Highly Flexible Scaffolds that Support Cell Proliferation in 3D

(2012) Li-Hsin Han et al.   ADVANCED FUNCTIONAL MATERIALS

Bioreactors to influence stem cell fate: Augmentation of mesenchymal stem cell signaling pathways via dynamic culture systems

(2012) Andrew B. Yeatts et al.   BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS

Microfabrication of complex porous tissue engineering scaffolds using 3D projection stereolithography

(2012) Robert Gauvin et al.   BIOMATERIALS

Guidance of stem cell fate on 2D patterned surfaces

(2012) Kristian Kolind et al.   BIOMATERIALS

Differences in growth properties of endometrial cancer in three dimensional (3D) culture and 2D cell monolayer

(2012) Kenny Chitcholtan et al.   EXPERIMENTAL CELL RESEARCH

Human adipose-derived stem cells and three-dimensional scaffold constructs: A review of the biomaterials and models currently used for bone regeneration

(2012) Andrea S. Zanetti et al.   JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS

Deconstructing the third dimension – how 3D culture microenvironments alter cellular cues

(2012) Brendon M. Baker et al.   JOURNAL OF CELL SCIENCE

Fabrication of functional three-dimensional tissues by stacking cell sheets in vitro

(2012) Yuji Haraguchi et al.   Nature Protocols

New dimensions in cell migration

(2012) Peter Friedl et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

3D Traction Forces in Cancer Cell Invasion

(2012) Thorsten M. Koch et al.   PLoS One

In vitro models for liver toxicity testing

(2012) Valerie Y. Soldatow et al.   Toxicology Research

Three-Dimensional Polymer Constructs Exhibiting a Tunable Negative Poisson's Ratio

(2011) David Y. Fozdar et al.   ADVANCED FUNCTIONAL MATERIALS

The use of nanoimprinted scaffolds as 3D culture models to facilitate spontaneous tumor cell migration and well-regulated spheroid formation

(2011) Yukie Yoshii et al.   BIOMATERIALS

Enhancing cell penetration and proliferation in chitosan hydrogels for tissue engineering applications

(2011) Chengdong Ji et al.   BIOMATERIALS

Construction of the recellularized corneal stroma using porous acellular corneal scaffold

(2011) Jianhui Xiao et al.   BIOMATERIALS

Micro-ring structures stabilize microdroplets to enable long term spheroid culture in 384 hanging drop array plates

(2011) Amy Y. Hsiao et al.   BIOMEDICAL MICRODEVICES

In vitro three-dimensional (3D) models in cancer research: An update

(2011) Lauren C. Kimlin et al.   MOLECULAR CARCINOGENESIS

Role of YAP/TAZ in mechanotransduction

(2011) Sirio Dupont et al.   NATURE

Kidney Spheroids Recapitulate Tubular Organoids Leading to Enhanced Tubulogenic Potency of Human Kidney-Derived Cells

(2011) Ella Buzhor et al.   TISSUE ENGINEERING PART A

From 3D cell culture to organs-on-chips

(2011) Dongeun Huh et al.   TRENDS IN CELL BIOLOGY

High-throughput 3D spheroid culture and drug testing using a 384 hanging drop array

(2010) Yi-Chung Tung et al.   ANALYST

The synergistic effects of 3-D porous silk fibroin matrix scaffold properties and hydrodynamic environment in cartilage tissue regeneration

(2010) Yun Wang et al.   BIOMATERIALS

Cell-laden microengineered gelatin methacrylate hydrogels

(2010) Jason W. Nichol et al.   BIOMATERIALS

Cartilage tissue engineering using funnel-like collagen sponges prepared with embossing ice particulate templates

(2010) Hongxu Lu et al.   BIOMATERIALS

The effect of matrix characteristics on fibroblast proliferation in 3D gels

(2010) Katrin Bott et al.   BIOMATERIALS

Tailoring the degradation kinetics of poly(ester carbonate urethane)urea thermoplastic elastomers for tissue engineering scaffolds

(2010) Yi Hong et al.   BIOMATERIALS

In vitrocartilage tissue engineering using cancellous bone matrix gelatin as a biodegradable scaffold

(2010) Bo Yang et al.   Biomedical Materials

Novel micro-bioreactor high throughput technology for cell culture process development: Reproducibility and scalability assessment of fed-batch CHO cultures

(2010) Ashraf Amanullah et al.   BIOTECHNOLOGY AND BIOENGINEERING

Peptide-Functionalized Click Hydrogels with Independently Tunable Mechanics and Chemical Functionality for 3D Cell Culture

(2010) Cole A. DeForest et al.   CHEMISTRY OF MATERIALS

Bioprinting Endothelial Cells With Alginate for 3D Tissue Constructs

(2010) Saif Khalil et al.   JOURNAL OF BIOMECHANICAL ENGINEERING-TRANSACTIONS OF THE ASME

Micropatterning as a tool to decipher cell morphogenesis and functions

(2010) M. Thery   JOURNAL OF CELL SCIENCE

Mechanical regulation of cell function with geometrically modulated elastomeric substrates

(2010) Jianping Fu et al.   NATURE METHODS

Reconstituting Organ-Level Lung Functions on a Chip

(2010) D. Huh et al.   SCIENCE

Uniform Beads with Controllable Pore Sizes for Biomedical Applications

(2010) Sung-Wook Choi et al.   Small

Cell-Laden Hydrogel Constructs of Hyaluronic Acid, Collagen, and Laminin for Neural Tissue Engineering

(2010) Shalu Suri et al.   TISSUE ENGINEERING PART A

Effect of Micro- and Macroporosity of Bone Tissue Three-Dimensional-Poly(ɛ-Caprolactone) Scaffold on Human Mesenchymal Stem Cells Invasion, Proliferation, and Differentiation In Vitro

(2010) Aurelio Salerno et al.   TISSUE ENGINEERING PART A

Direct Comparisons of the Morphology, Migration, Cell Adhesions, and Actin Cytoskeleton of Fibroblasts in Four Different Three-Dimensional Extracellular Matrices

(2010) Kirsi M. Hakkinen et al.   TISSUE ENGINEERING PART A

Fabrication of three-dimensional porous cell-laden hydrogel for tissue engineering

(2010) Chang Mo Hwang et al.   Biofabrication

Modular scaffolds assembled around living cells using poly(ethylene glycol) microspheres with macroporation via a non-cytotoxic porogen

(2009) Evan A. Scott et al.   Acta Biomaterialia

Hydrogels in Regenerative Medicine

(2009) Brandon V. Slaughter et al.   ADVANCED MATERIALS

Characterization of valvular interstitial cell function in three dimensional matrix metalloproteinase degradable PEG hydrogels

(2009) Julie A. Benton et al.   BIOMATERIALS

Patterning alginate hydrogels using light-directed release of caged calcium in a microfluidic device

(2009) Bor-han Chueh et al.   BIOMEDICAL MICRODEVICES

An open-access microfluidic model for lung-specific functional studies at an air-liquid interface

(2009) Divya D. Nalayanda et al.   BIOMEDICAL MICRODEVICES

A cell leakproof PLGA-collagen hybrid scaffold for cartilage tissue engineering

(2009) Naoki Kawazoe et al.   BIOTECHNOLOGY PROGRESS

Complexity in biomaterials for tissue engineering

(2009) Elsie S. Place et al.   NATURE MATERIALS

Sequential click reactions for synthesizing and patterning three-dimensional cell microenvironments

(2009) Cole A. DeForest et al.   NATURE MATERIALS

Photodegradable Hydrogels for Dynamic Tuning of Physical and Chemical Properties

(2009) April M. Kloxin et al.   SCIENCE

Tracheobronchial air-liquid interface cell culture: a model for innate mucosal defense of the upper airways?

(2008) Mehmet Kesimer et al.   AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY

The morphology of anisotropic 3D-printed hydroxyapatite scaffolds

(2008) Fabienne C. Fierz et al.   BIOMATERIALS

Stem cells and biomimetic materials strategies for tissue engineering

(2008) Susan Liao et al.   Materials Science & Engineering C-Materials for Biological Applications

Micro-bioreactor arrays for controlling cellular environments: Design principles for human embryonic stem cell applications

(2008) Elisa Cimetta et al.   METHODS

Small functional groups for controlled differentiation of hydrogel-encapsulated human mesenchymal stem cells

(2008) Danielle S. W. Benoit et al.   NATURE MATERIALS

Engineered Microenvironments for Controlled Stem Cell Differentiation

(2008) Jason A. Burdick et al.   TISSUE ENGINEERING PART A