A Critical Review of Genome Editing and Synthetic Biology Applications in Metabolic Engineering of Microalgae and Cyanobacteria

Improving the efficiency of photosynthetic carbon reactions

(2019) Andreas P.M. Weber et al.   PLANT PHYSIOLOGY

Synthetic glycolate metabolism pathways stimulate crop growth and productivity in the field

(2019) Paul F. South et al.   SCIENCE

Spatiotemporal Gene Repression System in the Heterocyst-Forming Multicellular Cyanobacterium Anabaena sp. PCC 7120

(2019) Akiyoshi Higo et al.   ACS Synthetic Biology

CyanoGate: A modular cloning suite for engineering cyanobacteria based on the plant MoClo syntax

(2019) Ravendran Vasudevan et al.   PLANT PHYSIOLOGY

On the use of oxygenic photosynthesis for the sustainable production of commodity chemicals

(2019) Adam A. Pérez et al.   PHYSIOLOGIA PLANTARUM

A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis

(2019) Xiaobo Li et al.   NATURE GENETICS

Efficient Editing of the Nuclear APT Reporter Gene in Chlamydomonas reinhardtii via Expression of a CRISPR-Cas9 Module

(2019) Daniel Guzmán-Zapata et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

CRISPR–Cas ribonucleoprotein mediated homology-directed repair for efficient targeted genome editing in microalgae Nannochloropsis oceanica IMET1

(2019) Mihris Ibnu Saleem Naduthodi et al.   Biotechnology for Biofuels

Overexpression of the DOF-Type Transcription Factor Enhances Lipid Synthesis in Chlorella vulgaris

(2019) Saki Tokunaga et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Application of the CRISPR/Cas system for genome editing in microalgae

(2019) Yu-Ting Zhang et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Synthetic Biology Toolkits for Metabolic Engineering of Cyanobacteria

(2019) Peng‐Fei Xia et al.   Biotechnology Journal

Systematic identification of a neutral site on chromosome of Synechococcus sp. PCC7002, a promising photosynthetic chassis strain

(2019) Min Wang et al.   JOURNAL OF BIOTECHNOLOGY

Increasing the specificity of CRISPR systems with engineered RNA secondary structures

(2019) D. Dewran Kocak et al.   NATURE BIOTECHNOLOGY

The lipid biochemistry of eukaryotic algae

(2019) Yonghua Li-Beisson et al.   PROGRESS IN LIPID RESEARCH

Fine-Tuning Native Promoters of Synechococcus elongatus PCC 7942 To Develop a Synthetic Toolbox for Heterologous Protein Expression

(2019) Annesha Sengupta et al.   ACS Synthetic Biology

Challenges and opportunity of recent genome editing and multi-omics in cyanobacteria and microalgae for biorefinery

(2019) Way-Rong Lin et al.   BIORESOURCE TECHNOLOGY

Selection of Cyanobacterial (Synechococcus sp. Strain PCC 6301) RubisCO Variants with Improved Functional Properties That Confer Enhanced CO2-Dependent Growth of Rhodobacter capsulatus, a Photosynthetic Bacterium

(2019) Sriram Satagopan et al.   mBio

Sequencing and comparative analysis of three Chlorella genomes provide insights into strain-specific adaptation to wastewater

(2019) Tian Wu et al.   Scientific Reports

Chlorella vulgaris genome assembly and annotation reveals the molecular basis for metabolic acclimation to high light conditions

(2019) Michela Cecchin et al.   PLANT JOURNAL

Glucose‐6‐Phosphate Dehydrogenase from the Oleaginous Microalga Nannochloropsis Uncovers Its Potential Role in Promoting Lipogenesis

(2019) Jiao Xue et al.   Biotechnology Journal

Metabolic model guided strain design of cyanobacteria

(2019) John I Hendry et al.   CURRENT OPINION IN BIOTECHNOLOGY

Toolboxes for cyanobacteria: Recent advances and future direction

(2018) Tao Sun et al.   BIOTECHNOLOGY ADVANCES

Recent developments in synthetic biology and metabolic engineering in microalgae towards biofuel production

(2018) Sheeja Jagadevan et al.   Biotechnology for Biofuels

Increasing the Photoautotrophic Growth Rate of Synechocystis sp. PCC 6803 by Identifying the Limitations of Its Cultivation

(2018) Pascal van Alphen et al.   Biotechnology Journal

Progress of CRISPR-Cas Based Genome Editing in Photosynthetic Microbes

(2018) Mihris Ibnu Saleem Naduthodi et al.   Biotechnology Journal

Analysis of Economic and Environmental Aspects of Microalgae Biorefinery for Biofuels Production: A Review

(2018) Shir Reen Chia et al.   Biotechnology Journal

Hijacking CRISPR-Cas for high-throughput bacterial metabolic engineering: advances and prospects

(2018) Ioannis Mougiakos et al.   CURRENT OPINION IN BIOTECHNOLOGY

Identification and Functional Analysis of the psaD Promoter of Chlorella vulgaris Using Heterologous Model Strains

(2018) Jongrae Kim et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

An inducible expression system in the alga Nannochloropsis gaditana controlled by the nitrate reductase promoter

(2018) Harry O. Jackson et al.   JOURNAL OF APPLIED PHYCOLOGY

Systematic overexpression study to find target enzymes enhancing production of terpenes in Synechocystis PCC 6803, using isoprene as a model compound

(2018) Elias Englund et al.   METABOLIC ENGINEERING

Development and optimization of genetic toolboxes for a fast-growing cyanobacterium Synechococcus elongatus UTEX 2973

(2018) Shubin Li et al.   METABOLIC ENGINEERING

Diversion of the long-chain acyl-ACP pool in Synechocystis to fatty alcohols through CRISPRi repression of the essential phosphate acyltransferase PlsX

(2018) Danuta Kaczmarzyk et al.   METABOLIC ENGINEERING

Engineered cyanobacteria with enhanced growth show increased ethanol production and higher biofuel to biomass ratio

(2018) Feiyan Liang et al.   METABOLIC ENGINEERING

Exploring native genetic elements as plug-in tools for synthetic biology in the cyanobacterium Synechocystis sp. PCC 6803

(2018) Deng Liu et al.   Microbial Cell Factories

Advanced genetic tools enable synthetic biology in the oleaginous microalgae Nannochloropsis sp.

(2018) Eric Poliner et al.   PLANT CELL REPORTS

High-throughput sequencing of the chloroplast and mitochondrion of Chlamydomonas reinhardtii to generate improved de novo assemblies, analyze expression patterns and transcript speciation, and evaluate diversity among laboratory strains and wild isolates

(2018) Sean D. Gallaher et al.   PLANT JOURNAL

Genome sequences of Chlorella sorokiniana UTEX 1602 and Micractinium conductrix SAG 241.80: implications to maltose excretion by a green alga

(2018) Matthew B. Arriola et al.   PLANT JOURNAL

Reversibility of citrate synthase allows autotrophic growth of a thermophilic bacterium

(2018) Achim Mall et al.   SCIENCE

CRISPR-Based Technologies for Metabolic Engineering in Cyanobacteria

(2018) Juliane Behler et al.   TRENDS IN BIOTECHNOLOGY

Augmenting the Calvin–Benson–Bassham cycle by a synthetic malyl-CoA-glycerate carbon fixation pathway

(2018) Hong Yu et al.   Nature Communications

An Expanded Plasmid-Based Genetic Toolbox Enables Cas9 Genome Editing and Stable Maintenance of Synthetic Pathways in Phaeodactylum tricornutum

(2018) Samuel S. Slattery et al.   ACS Synthetic Biology

Nontransgenic Marker-Free Gene Disruption by an Episomal CRISPR System in the Oleaginous Microalga, Nannochloropsis oceanica CCMP1779

(2018) Eric Poliner et al.   ACS Synthetic Biology

Targeted Repression of Essential Genes To Arrest Growth and Increase Carbon Partitioning and Biofuel Titers in Cyanobacteria

(2018) Kiyan Shabestary et al.   ACS Synthetic Biology

Increasing the Photoautotrophic Growth Rate of Synechocystis sp. PCC 6803 by Identifying the Limitations of Its Cultivation

(2018) Pascal van Alphen et al.   Biotechnology Journal

Progress of CRISPR-Cas Based Genome Editing in Photosynthetic Microbes

(2018) Mihris Ibnu Saleem Naduthodi et al.   Biotechnology Journal

Analysis of Economic and Environmental Aspects of Microalgae Biorefinery for Biofuels Production: A Review

(2018) Shir Reen Chia et al.   Biotechnology Journal

The primary transcriptome of the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973

(2018) Xiaoming Tan et al.   Biotechnology for Biofuels

Genome Annotation of a Model Diatom Phaeodactylum tricornutum Using an Integrated Proteogenomic Pipeline

(2018) Mingkun Yang et al.   Molecular Plant

Genome editing in diatoms: achievements and goals

(2018) Peter G. Kroth et al.   PLANT CELL REPORTS

Microalgae lipid and biomass for biofuel production: A comprehensive review on lipid enhancement strategies and their effects on fatty acid composition

(2018) Baharak Sajjadi et al.   RENEWABLE & SUSTAINABLE ENERGY REVIEWS

One-step generation of multiple gene knock-outs in the diatom Phaeodactylum tricornutum by DNA-free genome editing

(2018) Manuel Serif et al.   Nature Communications

Transgene-free genome editing in marine algae by bacterial conjugation – comparison with biolistic CRISPR/Cas9 transformation

(2018) A. K. Sharma et al.   Scientific Reports

Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii

(2018) Pierre Crozet et al.   ACS Synthetic Biology

Increased biomass and lipid production by continuous cultivation of Nannochloropsis salina transformant overexpressing a bHLH transcription factor

(2018) Nam Kyu Kang et al.   BIOTECHNOLOGY AND BIOENGINEERING

Cas9-guide RNA ribonucleoprotein-induced genome editing in the industrial green alga Coccomyxa sp. strain KJ

(2018) Yuya Yoshimitsu et al.   Biotechnology for Biofuels

Newly Identified Essential Amino Acids Affecting Chlorella ellipsoidea DGAT1 Function Revealed by Site-Directed Mutagenesis

(2018) Baocheng Sun et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Genome-scale fluxome of Synechococcus elongatus UTEX 2973 using transient 13C-labeling data

(2018) John I. Hendry et al.   PLANT PHYSIOLOGY

Terpenes and isoprenoids: a wealth of compounds for global use

(2018) Sarada D. Tetali   PLANTA

Engineering Microorganisms for Enhanced CO2 Sequestration

(2018) Guipeng Hu et al.   TRENDS IN BIOTECHNOLOGY

Manipulation of the microalgal chloroplast by genetic engineering for biotechnological utilization as a green biofactory

(2018) Yong Min Kwon et al.   WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

Expanding the Potential of CRISPR-Cpf1-Based Genome Editing Technology in the Cyanobacterium Anabaena PCC 7120

(2018) Tian-Cai Niu et al.   ACS Synthetic Biology

Genomic characterization reveals significant divergence within Chlorella sorokiniana (Chlorellales, Trebouxiophyceae)

(2018) Blake T. Hovde et al.   Algal Research-Biomass Biofuels and Bioproducts

Combining retinal-based and chlorophyll-based (oxygenic) photosynthesis: Proteorhodopsin expression increases growth rate and fitness of a ∆PSI strain of Synechocystis sp. PCC6803

(2018) Que Chen et al.   METABOLIC ENGINEERING

Predicting the metabolic capabilities of Synechococcus elongatus PCC 7942 adapted to different light regimes

(2018) Jared T. Broddrick et al.   METABOLIC ENGINEERING

Metabolic engineering of Synechococcus elongatus PCC 7942 for improvement of 1,3-propanediol and glycerol production based on in silico simulation of metabolic flux distribution

(2017) Yasutaka Hirokawa et al.   Microbial Cell Factories

Lipid production in Nannochloropsis gaditana is doubled by decreasing expression of a single transcriptional regulator

(2017) Imad Ajjawi et al.   NATURE BIOTECHNOLOGY

A toolkit for Nannochloropsis oceanica CCMP1779 enables gene stacking and genetic engineering of the eicosapentaenoic acid pathway for enhanced long-chain polyunsaturated fatty acid production

(2017) Eric Poliner et al.   PLANT BIOTECHNOLOGY JOURNAL

Targeting of Photoreceptor Genes inChlamydomonas reinhardtiivia Zinc-Finger Nucleases and CRISPR/Cas9

(2017) Andre Greiner et al.   PLANT CELL

Chlamydomonascarries out fatty acid β-oxidation in ancestral peroxisomes using a bona fide acyl-CoA oxidase

(2017) Fantao Kong et al.   PLANT JOURNAL

Efficient targeted DNA editing and replacement inChlamydomonas reinhardtiiusing Cpf1 ribonucleoproteins and single-stranded DNA

(2017) Aron Ferenczi et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Genetic Toolbox for Modulating the Expression of Heterologous Genes in the Cyanobacterium Synechocystis sp. PCC 6803

(2017) Bo Wang et al.   ACS Synthetic Biology

NOT Gate Genetic Circuits to Control Gene Expression in Cyanobacteria

(2017) Arnaud Taton et al.   ACS Synthetic Biology

Reconstruction and analysis of a genome-scale metabolic model of Nannochloropsis gaditana

(2017) Ab Rauf Shah et al.   Algal Research-Biomass Biofuels and Bioproducts

A gene-within-a-gene Cas9/sgRNA hybrid construct enables gene editing and gene replacement strategies in Chlamydomonas reinhardtii

(2017) Wen Zhi Jiang et al.   Algal Research-Biomass Biofuels and Bioproducts

Recent Developments on Genetic Engineering of Microalgae for Biofuels and Bio-Based Chemicals

(2017) I-Son Ng et al.   Biotechnology Journal

Expression of Synthetic Phytoene Synthase Gene to Enhance β-Carotene Production in Scenedesmus sp. CPC2

(2017) Chun-Yen Chen et al.   Biotechnology Journal

Development of SyneBrick Vectors As a Synthetic Biology Platform for Gene Expression in Synechococcus elongatus PCC 7942

(2017) Wook Jin Kim et al.   Frontiers in Plant Science

Identifying the Metabolic Differences of a Fast-Growth Phenotype in Synechococcus UTEX 2973

(2017) Thomas J. Mueller et al.   Scientific Reports

Towards developing algal synthetic biology

(2016) M. A. Scaife et al.   BIOCHEMICAL SOCIETY TRANSACTIONS

De novo transcriptomic analysis of Chlorella sorokiniana reveals differential genes expression in photosynthetic carbon fixation and lipid production

(2016) Lin Li et al.   BMC MICROBIOLOGY

Identification and Regulation of Genes for Cobalamin Transport in the Cyanobacterium Synechococcus sp. Strain PCC 7002

(2016) Adam A. Pérez et al.   JOURNAL OF BACTERIOLOGY

Genome Editing with CRISPR-Cas9: Can It Get Any Better?

(2016) Maximilian Haeussler et al.   Journal of Genetics and Genomics

CRISPR interference as a titratable, trans -acting regulatory tool for metabolic engineering in the cyanobacterium Synechococcus sp . strain PCC 7002

(2016) Gina C. Gordon et al.   METABOLIC ENGINEERING

CRISPR-Cas9 for the genome engineering of cyanobacteria and succinate production

(2016) Hung Li et al.   METABOLIC ENGINEERING

Reaction kinetic analysis of the 3-hydroxypropionate/4-hydroxybutyrate CO 2 fixation cycle in extremely thermoacidophilic archaea

(2016) Andrew J. Loder et al.   METABOLIC ENGINEERING

From cyanochemicals to cyanofactories: a review and perspective

(2016) Jie Zhou et al.   Microbial Cell Factories

CRISPR/Cas9 mediated targeted mutagenesis of the fast growing cyanobacterium Synechococcus elongatus UTEX 2973

(2016) Kristen E. Wendt et al.   Microbial Cell Factories

CRISPR interference (CRISPRi) for gene regulation and succinate production in cyanobacterium S. elongatus PCC 7942

(2016) Chun-Hung Huang et al.   Microbial Cell Factories

Genetic tools for advancement of Synechococcus sp. PCC 7002 as a cyanobacterial chassis

(2016) Anne M. Ruffing et al.   Microbial Cell Factories

Network analysis of transcriptomics expands regulatory landscapes inSynechococcussp. PCC 7002

(2016) Ryan S. McClure et al.   NUCLEIC ACIDS RESEARCH

Sustainable heterologous production of terpene hydrocarbons in cyanobacteria

(2016) Cinzia Formighieri et al.   PHOTOSYNTHESIS RESEARCH

An Indexed, Mapped Mutant Library Enables Reverse Genetics Studies of Biological Processes in Chlamydomonas reinhardtii

(2016) Xiaobo Li et al.   PLANT CELL

Genome editing of model oleaginous microalgaeNannochloropsisspp. by CRISPR/Cas9

(2016) Qintao Wang et al.   PLANT JOURNAL

A Comprehensively Curated Genome-Scale Two-Cell Model for the Heterocystous Cyanobacterium Anabaena sp. PCC 7120

(2016) David Malatinszky et al.   PLANT PHYSIOLOGY

Genome-Scale Model Reveals Metabolic Basis of Biomass Partitioning in a Model Diatom

(2016) Jennifer Levering et al.   PLoS One

Light-dependent chlorophyll f synthase is a highly divergent paralog of PsbA of photosystem II

(2016) M.-Y. Ho et al.   SCIENCE

A synthetic pathway for the fixation of carbon dioxide in vitro

(2016) Thomas Schwander et al.   SCIENCE

Synthetic promoters capable of driving robust nuclear gene expression in the green alga Chlamydomonas reinhardtii

(2016) Melissa A. Scranton et al.   Algal Research-Biomass Biofuels and Bioproducts

Genetic transformation of Nannochloropsis oculata with a bacterial phleomycin resistance gene as dominant selective marker

(2016) Xiaolei Ma et al.   Journal of Ocean University of China

Light-Harvesting in Photosynthesis

(2016) H. Scheer   ACTA PHYSICA POLONICA A

CRISPR/Cas9-induced knockout and knock-in mutations in Chlamydomonas reinhardtii

(2016) Sung-Eun Shin et al.   Scientific Reports

Evaluation of promoters and ribosome binding sites for biotechnological applications in the unicellular cyanobacterium Synechocystis sp. PCC 6803

(2016) Elias Englund et al.   Scientific Reports

Cpf1 Is A Versatile Tool for CRISPR Genome Editing Across Diverse Species of Cyanobacteria

(2016) Justin Ungerer et al.   Scientific Reports

Modelling metabolism of the diatom Phaeodactylum tricornutum

(2015) D. Singh et al.   BIOCHEMICAL SOCIETY TRANSACTIONS

Improving microalgae for biotechnology — From genetics to synthetic biology

(2015) Monika Hlavova et al.   BIOTECHNOLOGY ADVANCES

Construction of new synthetic biology tools for the control of gene expression in the cyanobacteriumSynechococcussp. strain PCC 7002

(2015) Erin K. Zess et al.   BIOTECHNOLOGY AND BIOENGINEERING

Cloning and characterization of a GPAT-like gene from the microalga Lobosphaera incisa (Trebouxiophyceae): overexpression in Chlamydomonas reinhardtii enhances TAG production

(2015) Umidjon Iskandarov et al.   JOURNAL OF APPLIED PHYCOLOGY

Effect of an Introduced Phytoene Synthase Gene Expression on Carotenoid Biosynthesis in the Marine Diatom Phaeodactylum tricornutum

(2015) Takashi Kadono et al.   Marine Drugs

Installing extra bicarbonate transporters in the cyanobacterium Synechocystis sp. PCC6803 enhances biomass production

(2015) Nina A. Kamennaya et al.   METABOLIC ENGINEERING

Genetic improvement of the microalga Phaeodactylum tricornutum for boosting neutral lipid accumulation

(2015) Jiao Xue et al.   METABOLIC ENGINEERING

Codon Bias as a Means to Fine-Tune Gene Expression

(2015) Tessa E.F. Quax et al.   MOLECULAR CELL

Flux balance analysis of primary metabolism in the diatomPhaeodactylum tricornutum

(2015) Joomi Kim et al.   PLANT JOURNAL

EstablishingChlamydomonas reinhardtiias an industrial biotechnology host

(2015) Mark A. Scaife et al.   PLANT JOURNAL

Characterization of cooperative bicarbonate uptake into chloroplast stroma in the green algaChlamydomonas reinhardtii

(2015) Takashi Yamano et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

In vivo Localization Studies in the Stramenopile Alga Nannochloropsis oceanica

(2015) Daniel Moog et al.   PROTIST

Designer diatom episomes delivered by bacterial conjugation

(2015) Bogumil J. Karas et al.   Nature Communications

Synechococcus elongatus UTEX 2973, a fast growing cyanobacterial chassis for biosynthesis using light and CO2

(2015) Jingjie Yu et al.   Scientific Reports

Multiple Gene Repression in Cyanobacteria Using CRISPRi

(2015) Lun Yao et al.   ACS Synthetic Biology

Development of a stable genetic system for Chlorella vulgaris —A promising green alga for CO 2 biomitigation

(2015) Bo Yang et al.   Algal Research-Biomass Biofuels and Bioproducts

Purple Chromoprotein Gene Serves as a New Selection Marker for Transgenesis of the Microalga Nannochloropsis oculata

(2015) Chen-Han Shih et al.   PLoS One

Regulation of Gene Expression in Diverse Cyanobacterial Species by Using Theophylline-Responsive Riboswitches

(2014) Amy T. Ma et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

The optimization of biomass and lipid yields of Chlorella sorokiniana when using wastewater supplemented with different nitrogen sources

(2014) Luveshan Ramanna et al.   BIORESOURCE TECHNOLOGY

High-efficiency nuclear transformation of the oleaginous marineNannochloropsisspecies using PCR product

(2014) Fengjuan Li et al.   BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY

Exploring metabolic engineering design principles for the photosynthetic production of lactic acid by Synechocystis sp. PCC6803

(2014) S Angermayr et al.   Biotechnology for Biofuels

Comparative Analysis of the Primary Transcriptome of Synechocystis sp. PCC 6803

(2014) Matthias Kopf et al.   DNA RESEARCH

Successful Transient Expression of Cas9 and Single Guide RNA Genes in Chlamydomonas reinhardtii

(2014) Wenzhi Jiang et al.   EUKARYOTIC CELL

Construction of Novel Chloroplast Expression Vector and Development of an Efficient Transformation System for the Diatom Phaeodactylum tricornutum

(2014) Wei-Hong Xie et al.   MARINE BIOTECHNOLOGY

Characterization of iron-responsive promoters in the marine diatom Phaeodactylum tricornutum

(2014) Ryo Yoshinaga et al.   Marine Genomics

High-Throughput Genotyping of Green Algal Mutants Reveals Random Distribution of Mutagenic Insertion Sites and Endonucleolytic Cleavage of Transforming DNA

(2014) R. Zhang et al.   PLANT CELL

Enhanced Genetic Tools for Engineering Multigene Traits into Green Algae

(2014) Beth A. Rasala et al.   PLoS One

Genome engineering empowers the diatom Phaeodactylum tricornutum for biotechnology

(2014) Fayza Daboussi et al.   Nature Communications

Genome-wide identification of transcription factors and transcription-factor binding sites in oleaginous microalgae Nannochloropsis

(2014) Jianqiang Hu et al.   Scientific Reports

Synthetic Biology Toolbox for Controlling Gene Expression in the Cyanobacterium Synechococcus sp. strain PCC 7002

(2014) Andrew L. Markley et al.   ACS Synthetic Biology

Nannochloropsis Genomes Reveal Evolution of Microalgal Oleaginous Traits

(2014) Dongmei Wang et al.   PLoS Genetics

Expression of bovine lactoferrin N-lobe by the green alga, Chlorella vulgaris

(2014) Jungmo Koo et al.   ALGAE

Highly Efficient Transformation of the DiatomPhaeodactylum tricornutumby Multi-Pulse Electroporation

(2013) Mado MIYAHARA et al.   BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY

Computational evaluation ofSynechococcussp. PCC 7002 metabolism for chemical production

(2013) Trang T. Vu et al.   Biotechnology Journal

The Response of Nannochloropsis gaditana to Nitrogen Starvation Includes De Novo Biosynthesis of Triacylglycerols, a Decrease of Chloroplast Galactolipids, and Reorganization of the Photosynthetic Apparatus

(2013) Diana Simionato et al.   EUKARYOTIC CELL

High-value products from microalgae—their development and commercialisation

(2013) Michael A. Borowitzka   JOURNAL OF APPLIED PHYCOLOGY

Proteomic analysis of Chlorella vulgaris: Potential targets for enhanced lipid accumulation

(2013) Michael T. Guarnieri et al.   Journal of Proteomics

Improvement of Neutral Lipid and Polyunsaturated Fatty Acid Biosynthesis by Overexpressing a Type 2 Diacylglycerol Acyltransferase in Marine Diatom Phaeodactylum tricornutum

(2013) Ying-Fang Niu et al.   Marine Drugs

High-efficiency nuclear transformation of the diatom Phaeodactylum tricornutum by electroporation

(2013) Chunye Zhang et al.   Marine Genomics

Metabolic engineering of Phaeodactylum tricornutum for the enhanced accumulation of omega-3 long chain polyunsaturated fatty acids

(2013) Mary L. Hamilton et al.   METABOLIC ENGINEERING

The rrnA promoter as a tool for the improved expression of heterologous genes in cyanobacteria

(2013) Wipa Chungjatupornchai et al.   MICROBIOLOGICAL RESEARCH

Genetic manipulation, a feasible tool to enhance unique characteristic of Chlorella vulgaris as a feedstock for biodiesel production

(2013) Ahmad Farhad Talebi et al.   MOLECULAR BIOLOGY REPORTS

Chromosome Scale Genome Assembly and Transcriptome Profiling of Nannochloropsis gaditana in Nitrogen Depletion

(2013) Elisa Corteggiani Carpinelli et al.   Molecular Plant

Theophylline-Dependent Riboswitch as a Novel Genetic Tool for Strict Regulation of Protein Expression in Cyanobacterium Synechococcus elongatus PCC 7942

(2013) Yoichi Nakahira et al.   PLANT AND CELL PHYSIOLOGY

Correction: Aureochrome 1a Is Involved in the Photoacclimation of the Diatom Phaeodactylum tricornutum

(2013) Benjamin Schellenberger Costa et al.   PLoS One

An Organic Acid Based Counter Selection System for Cyanobacteria

(2013) Matthew B. Begemann et al.   PLoS One

Metabolic engineering of lipid catabolism increases microalgal lipid accumulation without compromising growth

(2013) E. M. Trentacoste et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cyanobacterial conversion of carbon dioxide to 2,3-butanediol

(2013) John W. K. Oliver et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Flux Balance Analysis of Cyanobacterial Metabolism: The Metabolic Network of Synechocystis sp. PCC 6803

(2013) Henning Knoop et al.   PLoS Computational Biology

Synthetic biology of cyanobacteria: unique challenges and opportunities

(2013) Bertram M. Berla et al.   Frontiers in Microbiology

Wide-dynamic-range promoters engineered for cyanobacteria

(2013) Hsin-Ho Huang et al.   Journal of Biological Engineering

Engineering of a green-light inducible gene expression system inSynechocystissp. PCC6803

(2013) Koichi Abe et al.   Microbial Biotechnology

Design and analysis of metabolic pathways supporting formatotrophic growth for electricity-dependent cultivation of microbes

(2012) Arren Bar-Even et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS

Metabolic shifts: a fitness perspective for microbial cell factories

(2012) Anisha Goel et al.   BIOTECHNOLOGY LETTERS

Flux balance analysis of primary metabolism in Chlamydomonas reinhardtii

(2012) Nanette R Boyle et al.   BMC Systems Biology

The Redox-sensing Transcriptional Regulator RexT Controls Expression of Thioredoxin A2 in the CyanobacteriumAnabaenasp. Strain PCC 7120

(2012) Shigeki Ehira et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Functional analysis of three type-2 DGAT homologue genes for triacylglycerol production in the green microalga Chlamydomonas reinhardtii

(2012) M. La Russa et al.   JOURNAL OF BIOTECHNOLOGY

The Roles of acyl-CoA: Diacylglycerol Acyltransferase 2 Genes in the Biosynthesis of Triacylglycerols by the Green Algae Chlamydomonas reinhardtii

(2012) Xiao-Dong Deng et al.   Molecular Plant

Nuclear gene targeting inChlamydomonasusing engineered zinc-finger nucleases

(2012) Irina Sizova et al.   PLANT JOURNAL

Identification of Functional Differences in Metabolic Networks Using Comparative Genomics and Constraint-Based Models

(2012) Joshua J. Hamilton et al.   PLoS One

Draft genome sequence and genetic transformation of the oleaginous alga Nannochloropsis gaditana

(2012) Randor Radakovits et al.   Nature Communications

Genome, Functional Gene Annotation, and Nuclear Transformation of the Heterokont Oleaginous Alga Nannochloropsis oceanica CCMP1779

(2012) Astrid Vieler et al.   PLoS Genetics

Acclimation of the Global Transcriptome of the Cyanobacterium Synechococcus sp. Strain PCC 7002 to Nutrient Limitations and Different Nitrogen Sources

(2012) Marcus Ludwig et al.   Frontiers in Microbiology

Flux coupling and transcriptional regulation within the metabolic network of the photosynthetic bacterium Synechocystis sp. PCC6803

(2011) Arnau Montagud et al.   Biotechnology Journal

AlgaGEM – a genome-scale metabolic reconstruction of algae based on the Chlamydomonas reinhardtii genome

(2011) Cristiana Gomes de Oliveira Dal’Molin et al.   BMC GENOMICS

Novel Shuttle Markers for Nuclear Transformation of the Green Alga Chlamydomonas reinhardtii

(2011) Laurence Meslet-Cladière et al.   EUKARYOTIC CELL

A chloroplast pathway for the de novo biosynthesis of triacylglycerol inChlamydomonas reinhardtii

(2011) Jilian Fan et al.   FEBS LETTERS

A new inducible expression system in a transformed green alga, Chlorella vulgaris

(2011) Y.F. Niu et al.   GENETICS AND MOLECULAR RESEARCH

Cinnamic acid, coumarin and vanillin: Alternative phenolic compounds for efficient Agrobacterium-mediated transformation of the unicellular green alga, Nannochloropsis sp.

(2011) Thye-San Cha et al.   JOURNAL OF MICROBIOLOGICAL METHODS

Metabolic network reconstruction of Chlamydomonas offers insight into light-driven algal metabolism

(2011) R. L. Chang et al.   Molecular Systems Biology

Examination of Triacylglycerol Biosynthetic Pathways via De Novo Transcriptomic and Proteomic Analyses in an Unsequenced Microalga

(2011) Michael T. Guarnieri et al.   PLoS One

Assessment of factors affecting Agrobacterium-mediated genetic transformation of the unicellular green alga, Chlorella vulgaris

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Engineering Cyanobacteria To Synthesize and Export Hydrophilic Products

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Improved Eco-Friendly RecombinantAnabaenasp. Strain PCC7120 with Enhanced Nitrogen Biofertilizer Potential

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A perspective: Photosynthetic production of fatty acid-based biofuels in genetically engineered cyanobacteria

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Targeting DNA Double-Strand Breaks with TAL Effector Nucleases

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Production of therapeutic proteins in algae, analysis of expression of seven human proteins in the chloroplast of Chlamydomonas reinhardtii

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The Prospect of Using Cyanobacterial Bicarbonate Transporters to Improve Leaf Photosynthesis in C3 Crop Plants

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The Metabolic Network of Synechocystis sp. PCC 6803: Systemic Properties of Autotrophic Growth

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Enhancement of lipid production using biochemical, genetic and transcription factor engineering approaches

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Genome-scale modeling ofSynechocystissp. PCC 6803 and prediction of pathway insertion

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The Phaeodactylum genome reveals the evolutionary history of diatom genomes

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