One-step generation of multiple gene knock-outs in the diatom Phaeodactylum tricornutum by DNA-free genome editing

An Expanded Plasmid-Based Genetic Toolbox Enables Cas9 Genome Editing and Stable Maintenance of Synthetic Pathways in Phaeodactylum tricornutum

(2018) Samuel S. Slattery et al.   ACS Synthetic Biology

PtAUREO1a and PtAUREO1b knockout mutants of the diatom Phaeodactylum tricornutum are blocked in photoacclimation to blue light

(2017) Marcus Mann et al.   JOURNAL OF PLANT PHYSIOLOGY

Genetic and metabolic engineering in diatoms

(2017) Weichao Huang et al.   PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES

Efficient targeted DNA editing and replacement inChlamydomonas reinhardtiiusing Cpf1 ribonucleoproteins and single-stranded DNA

(2017) Aron Ferenczi et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A fast and reliable strategy to generate TALEN-mediated gene knockouts in the diatom Phaeodactylum tricornutum

(2017) M. Serif et al.   Algal Research-Biomass Biofuels and Bioproducts

Multisignal control of expression of the LHCX protein family in the marine diatomPhaeodactylum tricornutum

(2016) Lucilla Taddei et al.   JOURNAL OF EXPERIMENTAL BOTANY

CRISPR-Cas9-mediated efficient directed mutagenesis and RAD51-dependent and RAD51-independent gene targeting in the mossPhyscomitrella patens

(2016) Cécile Collonnier et al.   PLANT BIOTECHNOLOGY JOURNAL

Diatom Phytochromes Reveal the Existence of Far-Red-Light-Based Sensing in the Ocean

(2016) Antonio Emidio Fortunato et al.   PLANT CELL

Analysis of Repair Mechanisms following an Induced Double-Strand Break Uncovers Recessive Deleterious Alleles in the Candida albicans Diploid Genome

(2016) Adeline Feri et al.   mBio

A CRISPR/Cas9 system adapted for gene editing in marine algae

(2016) Marianne Nymark et al.   Scientific Reports

Photosynthetic Pigments in Diatoms

(2015) Paulina Kuczynska et al.   Marine Drugs

Towards the Industrial Production of Omega-3 Long Chain Polyunsaturated Fatty Acids from a Genetically Modified Diatom Phaeodactylum tricornutum

(2015) Mary L. Hamilton et al.   PLoS One

Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins

(2014) S. Kim et al.   GENOME RESEARCH

A guide to genome engineering with programmable nucleases

(2014) Hyongbum Kim et al.   NATURE REVIEWS GENETICS

Easy quantitative assessment of genome editing by sequence trace decomposition

(2014) Eva K. Brinkman et al.   NUCLEIC ACIDS RESEARCH

Inactivation of Phaeodactylum tricornutum urease gene using transcription activator-like effector nuclease-based targeted mutagenesis

(2014) Philip D. Weyman et al.   PLANT BIOTECHNOLOGY JOURNAL

Proteolistics: a biolistic method for intracellular delivery of proteins

(2014) Susana Martin-Ortigosa et al.   TRANSGENIC RESEARCH

Genome engineering empowers the diatom Phaeodactylum tricornutum for biotechnology

(2014) Fayza Daboussi et al.   Nature Communications

AUREOCHROME1a-Mediated Induction of the Diatom-Specific CyclindsCYC2Controls the Onset of Cell Division in Diatoms (Phaeodactylum tricornutum)

(2013) Marie J.J. Huysman et al.   PLANT CELL

TAL Effector-Nucleotide Targeter (TALE-NT) 2.0: tools for TAL effector design and target prediction

(2012) Erin L. Doyle et al.   NUCLEIC ACIDS RESEARCH

Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting

(2011) Tomas Cermak et al.   NUCLEIC ACIDS RESEARCH

Identification of the UMP Synthase Gene by Establishment of Uracil Auxotrophic Mutants and the Phenotypic Complementation System in the Marine Diatom Phaeodactylum tricornutum

(2011) T. Sakaguchi et al.   PLANT PHYSIOLOGY