Application of the CRISPR/Cas system for genome editing in microalgae

CRISPR/Cas9-based genome-editing in the filamentous fungus Fusarium fujikuroi and its application in strain engineering for gibberellic acid production

(2019) Tian-Qiong Shi et al.   ACS Synthetic Biology

Transformation techniques for metabolic engineering of diatoms and haptophytes: current state and prospects

(2018) Natarajan Velmurugan et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Development of a cooperative two-factor adaptive-evolution method to enhance lipid production and prevent lipid peroxidation in Schizochytrium sp.

(2018) Xiao-Man Sun et al.   Biotechnology for Biofuels

Nontransgenic Marker-Free Gene Disruption by an Episomal CRISPR System in the Oleaginous Microalga, Nannochloropsis oceanica CCMP1779

(2018) Eric Poliner et al.   ACS Synthetic Biology

Advancing metabolic engineering of Yarrowia lipolytica using the CRISPR/Cas system

(2018) Tian-Qiong Shi et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

One-step generation of multiple gene knock-outs in the diatom Phaeodactylum tricornutum by DNA-free genome editing

(2018) Manuel Serif et al.   Nature Communications

CRISPR-GE: A Convenient Software Toolkit for CRISPR-Based Genome Editing

(2017) Xianrong Xie et al.   Molecular Plant

Efficient targeted DNA editing and replacement inChlamydomonas reinhardtiiusing Cpf1 ribonucleoproteins and single-stranded DNA

(2017) Aron Ferenczi et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A fast and reliable strategy to generate TALEN-mediated gene knockouts in the diatom Phaeodactylum tricornutum

(2017) M. Serif et al.   Algal Research-Biomass Biofuels and Bioproducts

CRISPR interference as a titratable, trans -acting regulatory tool for metabolic engineering in the cyanobacterium Synechococcus sp . strain PCC 7002

(2016) Gina C. Gordon et al.   METABOLIC ENGINEERING

CRISPR-Cas9 for the genome engineering of cyanobacteria and succinate production

(2016) Hung Li et al.   METABOLIC ENGINEERING

CRISPR/Cas9 mediated targeted mutagenesis of the fast growing cyanobacterium Synechococcus elongatus UTEX 2973

(2016) Kristen E. Wendt et al.   Microbial Cell Factories

CRISPR interference (CRISPRi) for gene regulation and succinate production in cyanobacterium S. elongatus PCC 7942

(2016) Chun-Hung Huang et al.   Microbial Cell Factories

Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage

(2016) Alexis C. Komor et al.   NATURE

Genome editing of model oleaginous microalgaeNannochloropsisspp. by CRISPR/Cas9

(2016) Qintao Wang et al.   PLANT JOURNAL

Editing of the urease gene by CRISPR-Cas in the diatom Thalassiosira pseudonana

(2016) Amanda Hopes et al.   Plant Methods

Diverse evolutionary roots and mechanistic variations of the CRISPR-Cas systems

(2016) Prarthana Mohanraju et al.   SCIENCE

DNA-free two-gene knockout in Chlamydomonas reinhardtii via CRISPR-Cas9 ribonucleoproteins

(2016) Kwangryul Baek et al.   Scientific Reports

A CRISPR/Cas9 system adapted for gene editing in marine algae

(2016) Marianne Nymark et al.   Scientific Reports

Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi)

(2016) Sara Cleto et al.   ACS Synthetic Biology

CRISPR/Cas9-induced knockout and knock-in mutations in Chlamydomonas reinhardtii

(2016) Sung-Eun Shin et al.   Scientific Reports

Cpf1 Is A Versatile Tool for CRISPR Genome Editing Across Diverse Species of Cyanobacteria

(2016) Justin Ungerer et al.   Scientific Reports

Highly efficient editing of the actinorhodin polyketide chain length factor gene in Streptomyces coelicolor M145 using CRISPR/Cas9-CodA(sm) combined system

(2015) Hu Zeng et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

“Off-Spotter”: very fast and exhaustive enumeration of genomic lookalikes for designing CRISPR/Cas guide RNAs

(2015) Venetia Pliatsika et al.   Biology Direct

Improving high carbon dioxide tolerance and carbon dioxide fixation capability of Chlorella sp. by adaptive laboratory evolution

(2015) Dengjin Li et al.   BIORESOURCE TECHNOLOGY

Marine Indole Alkaloids

(2015) Natalie Netz et al.   Marine Drugs

Multiplex metabolic pathway engineering using CRISPR/Cas9 in Saccharomyces cerevisiae

(2015) Tadas Jakočiūnas et al.   METABOLIC ENGINEERING

The New State of the Art: Cas9 for Gene Activation and Repression

(2015) Marie F. La Russa et al.   MOLECULAR AND CELLULAR BIOLOGY

Discovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems

(2015) Sergey Shmakov et al.   MOLECULAR CELL

Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex

(2015) Silvana Konermann et al.   NATURE

In vivo genome editing using Staphylococcus aureus Cas9

(2015) F. Ann Ran et al.   NATURE

Multiple Gene Repression in Cyanobacteria Using CRISPRi

(2015) Lun Yao et al.   ACS Synthetic Biology

Genome-Wide Tuning of Protein Expression Levels to Rapidly Engineer Microbial Traits

(2015) Emily F. Freed et al.   ACS Synthetic Biology

Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases

(2014) S. Bae et al.   BIOINFORMATICS

Gene inactivation using the CRISPR/Cas9 system in the nematode Pristionchus pacificus

(2014) Hanh Witte et al.   DEVELOPMENT GENES AND EVOLUTION

Successful Transient Expression of Cas9 and Single Guide RNA Genes in Chlamydomonas reinhardtii

(2014) Wenzhi Jiang et al.   EUKARYOTIC CELL

Efficient search, mapping, and optimization of multi-protein genetic systems in diverse bacteria

(2014) I. Farasat et al.   Molecular Systems Biology

Rational design of highly active sgRNAs for CRISPR-Cas9–mediated gene inactivation

(2014) John G Doench et al.   NATURE BIOTECHNOLOGY

E-CRISP: fast CRISPR target site identification

(2014) Florian Heigwer et al.   NATURE METHODS

Synthetic Biology Toolbox for Controlling Gene Expression in the Cyanobacterium Synechococcus sp. strain PCC 7002

(2014) Andrew L. Markley et al.   ACS Synthetic Biology

TALE activation of endogenous genes in Chlamydomonas reinhardtii

(2014) Han Gao et al.   Algal Research-Biomass Biofuels and Bioproducts

Erratum to: ku70 and ku80 null mutants improve the gene targeting frequency in Monascus ruber M7

(2013) Yi He et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

(2013) Lei S. Qi et al.   CELL

DNA targeting specificity of RNA-guided Cas9 nucleases

(2013) Patrick D Hsu et al.   NATURE BIOTECHNOLOGY

CRISPR interference (CRISPRi) for sequence-specific control of gene expression

(2013) Matthew H Larson et al.   Nature Protocols

Studies on Recombination Processes in two Chlamydomonas reinhardtii Endogenous Genes, NIT1 and ARG7

(2013) Andrea Plecenikova et al.   PROTIST

ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering

(2013) Thomas Gaj et al.   TRENDS IN BIOTECHNOLOGY

Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas

(2013) Fahim Farzadfard et al.   ACS Synthetic Biology

Harvesting of microalgae by flocculation with poly (γ-glutamic acid)

(2012) Hongli Zheng et al.   BIORESOURCE TECHNOLOGY

The Crystal Structure of TAL Effector PthXo1 Bound to Its DNA Target

(2012) A. N.-S. Mak et al.   SCIENCE

High-efficiency homologous recombination in the oil-producing alga Nannochloropsis sp.

(2011) O. Kilian et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Developments and perspectives of photobioreactors for biofuel production

(2010) Michael Morweiser et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Red microalgal cell-wall polysaccharides: biotechnological aspects

(2010) Shoshana (Malis) Arad et al.   CURRENT OPINION IN BIOTECHNOLOGY

Increased Lipid Accumulation in the Chlamydomonas reinhardtiista7-10Starchless Isoamylase Mutant and Increased Carbohydrate Synthesis in Complemented Strains

(2010) Victoria H. Work et al.   EUKARYOTIC CELL

Genetic Engineering of Algae for Enhanced Biofuel Production

(2010) Randor Radakovits et al.   EUKARYOTIC CELL

TheChlorella variabilisNC64A Genome Reveals Adaptation to Photosymbiosis, Coevolution with Viruses, and Cryptic Sex

(2010) Guillaume Blanc et al.   PLANT CELL

Knockout of the DNA ligase IV homolog gene in the sphingoid base producing yeast Pichia ciferrii significantly increases gene targeting efficiency

(2009) Christoph Schorsch et al.   CURRENT GENETICS

The Phaeodactylum genome reveals the evolutionary history of diatom genomes

(2008) Chris Bowler et al.   NATURE