Manipulation of the microalgal chloroplast by genetic engineering for biotechnological utilization as a green biofactory

Engineering the Chloroplast Genome of Oleaginous Marine Microalga Nannochloropsis oceanica

(2018) Qinhua Gan et al.   Frontiers in Plant Science

The monoplastidic bottleneck in algae and plant evolution

(2017) Jan de Vries et al.   JOURNAL OF CELL SCIENCE

Synthesis of bacteriophage lytic proteins against Streptococcus pneumoniae in the chloroplast of Chlamydomonas reinhardtii

(2017) Laura Stoffels et al.   PLANT BIOTECHNOLOGY JOURNAL

High-yield secretion of recombinant proteins from the microalga Chlamydomonas reinhardtii

(2017) Erick Miguel Ramos-Martinez et al.   PLANT BIOTECHNOLOGY JOURNAL

Chromosome-level genome assembly and transcriptome of the green algaChromochloris zofingiensisilluminates astaxanthin production

(2017) Melissa S. Roth et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Microalgae and cyanobacteria as enzyme biofactories

(2017) Bruno dos Santos Alves Figueiredo Brasil et al.   Algal Research-Biomass Biofuels and Bioproducts

Recent Developments on Genetic Engineering of Microalgae for Biofuels and Bio-Based Chemicals

(2017) I-Son Ng et al.   Biotechnology Journal

Green biologics: The algal chloroplast as a platform for making biopharmaceuticals

(2017) Henry N. Taunt et al.   Bioengineered

New tools for chloroplast genetic engineering allow the synthesis of human growth hormone in the green alga Chlamydomonas reinhardtii

(2016) Thanyanan Wannathong et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Outlook in the application of Chlamydomonas reinhardtii chloroplast as a platform for recombinant protein production

(2016) Shabnam Shamriz et al.   Biotechnology & Genetic Engineering Reviews

Toward mosquito control with a green alga: expression of Cry toxins of Bacillus thuringiensis subsp. israelensis (Bti) in the chloroplast of Chlamydomonas

(2016) Seongjoon Kang et al.   JOURNAL OF APPLIED PHYCOLOGY

Bioactivity of an antihypertensive peptide expressed in Chlamydomonas reinhardtii

(2016) Celma Estefanía Ochoa-Méndez et al.   JOURNAL OF BIOTECHNOLOGY

Genome editing of model oleaginous microalgaeNannochloropsisspp. by CRISPR/Cas9

(2016) Qintao Wang et al.   PLANT JOURNAL

Extending the biosynthetic repertoires of cyanobacteria and chloroplasts

(2016) Agnieszka Zygadlo Nielsen et al.   PLANT JOURNAL

DNA-free two-gene knockout in Chlamydomonas reinhardtii via CRISPR-Cas9 ribonucleoproteins

(2016) Kwangryul Baek et al.   Scientific Reports

A CRISPR/Cas9 system adapted for gene editing in marine algae

(2016) Marianne Nymark et al.   Scientific Reports

siRNA and RNAi optimization

(2016) Adele Alagia et al.   Wiley Interdisciplinary Reviews-RNA

Transgene Expression in Microalgae—From Tools to Applications

(2016) Lior Doron et al.   Frontiers in Plant Science

Stable Nuclear Transformation System for the Coccolithophorid Alga Pleurochrysis carterae

(2016) Hirotoshi Endo et al.   Scientific Reports

CRISPR/Cas9-induced knockout and knock-in mutations in Chlamydomonas reinhardtii

(2016) Sung-Eun Shin et al.   Scientific Reports

Improving recombinant protein production in theChlamydomonas reinhardtiichloroplast using vivid Verde Fluorescent Protein as a reporter

(2015) Stephanie Braun-Galleani et al.   Biotechnology Journal

Biotechnological exploitation of microalgae

(2015) Doris Gangl et al.   JOURNAL OF EXPERIMENTAL BOTANY

Expression and membrane-targeting of an active plant cytochrome P450 in the chloroplast of the green alga Chlamydomonas reinhardtii

(2015) Doris Gangl et al.   PHYTOCHEMISTRY

Improving recombinant protein production in theChlamydomonas reinhardtiichloroplast using vivid Verde Fluorescent Protein as a reporter

(2015) Stephanie Braun-Galleani et al.   Biotechnology Journal

In Metabolic Engineering of Eukaryotic Microalgae: Potential and Challenges Come with Great Diversity

(2015) Javier A. Gimpel et al.   Frontiers in Microbiology

Cloning Should Be Simple: Escherichia coli DH5α-Mediated Assembly of Multiple DNA Fragments with Short End Homologies

(2015) Maxim Kostylev et al.   PLoS One

Transgenic Expression of a Bacterial Thermophilic Amylase in the Chlamydomonas reinhardtii Chloroplast to Facilitate Algal Biofuel Production

(2014) Xiaoqing Wang et al.   BioEnergy Research

High-efficiency nuclear transformation of the oleaginous marineNannochloropsisspecies using PCR product

(2014) Fengjuan Li et al.   BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY

Stable expression of a bifunctional diterpene synthase in the chloroplast of Chlamydomonas reinhardtii

(2014) Julie A. Z. Zedler et al.   JOURNAL OF APPLIED PHYCOLOGY

Photosynthetic biomanufacturing in green algae; production of recombinant proteins for industrial, nutritional, and medical uses

(2014) Beth A. Rasala et al.   PHOTOSYNTHESIS RESEARCH

Algal chloroplast produced camelid VH H antitoxins are capable of neutralizing botulinum neurotoxin

(2014) Daniel J. Barrera et al.   PLANT BIOTECHNOLOGY JOURNAL

TALE activation of endogenous genes in Chlamydomonas reinhardtii

(2014) Han Gao et al.   Algal Research-Biomass Biofuels and Bioproducts

Recent advances in the study of chloroplast gene expression and its evolution

(2014) Yusuke Yagi et al.   Frontiers in Plant Science

Marker-free genetic engineering of the chloroplast in the green microalgaChlamydomonas reinhardtii

(2013) Hsu-Ching Chen et al.   PLANT BIOTECHNOLOGY JOURNAL

Synthetic biology in plastids

(2013) Lars B. Scharff et al.   PLANT JOURNAL

A Chlamydomonas-Derived Human Papillomavirus 16 E7 Vaccine Induces Specific Tumor Protection

(2013) Olivia C. Demurtas et al.   PLoS One

Genetic engineering of algal chloroplasts: Progress and prospects

(2013) S. Purton et al.   RUSSIAN JOURNAL OF PLANT PHYSIOLOGY

ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering

(2013) Thomas Gaj et al.   TRENDS IN BIOTECHNOLOGY

HOW MANY SPECIES OF ALGAE ARE THERE?

(2012) Michael D. Guiry   JOURNAL OF PHYCOLOGY

Nuclear gene targeting inChlamydomonasusing engineered zinc-finger nucleases

(2012) Irina Sizova et al.   PLANT JOURNAL

Algae-Produced Pfs25 Elicits Antibodies That Inhibit Malaria Transmission

(2012) James A. Gregory et al.   PLoS One

Production of unique immunotoxin cancer therapeutics in algal chloroplasts

(2012) M. Tran et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Synthetic Oligonucleotide Libraries Reveal Novel Regulatory Elements in Chlamydomonas Chloroplast mRNAs

(2012) Elizabeth A. Specht et al.   ACS Synthetic Biology

Production of recombinant enzymes in the marine alga Dunaliella tertiolecta

(2012) D. Ryan Georgianna et al.   Algal Research-Biomass Biofuels and Bioproducts

Transgenic microalgae expressing Escherichia coli AppA phytase as feed additive to reduce phytate excretion in the manure of young broiler chicks

(2011) So-Mi Yoon et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

The chloroplast transformation toolbox: selectable markers and marker removal

(2011) Anil Day et al.   PLANT BIOTECHNOLOGY JOURNAL

Chlamydomonas reinhardtii as a viable platform for the production of recombinant proteins: current status and perspectives

(2011) Sergio Rosales-Mendoza et al.   PLANT CELL REPORTS

Expression of Plastid Genes: Organelle-Specific Elaborations on a Prokaryotic Scaffold

(2011) A. Barkan   PLANT PHYSIOLOGY

High-efficiency homologous recombination in the oil-producing alga Nannochloropsis sp.

(2011) O. Kilian et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Overexpression of an exogenous phytoene synthase gene in the unicellular alga Chlamydomonas reinhardtii leads to an increase in the content of carotenoids

(2010) I. Couso et al.   BIOTECHNOLOGY PROGRESS

Enhanced chloroplast transgene expression in a nuclear mutant of Chlamydomonas

(2010) Laure Michelet et al.   PLANT BIOTECHNOLOGY JOURNAL

Production of therapeutic proteins in algae, analysis of expression of seven human proteins in the chloroplast of Chlamydomonas reinhardtii

(2010) Beth A. Rasala et al.   PLANT BIOTECHNOLOGY JOURNAL

Factors effecting expression of vaccines in microalgae

(2009) Raymond Surzycki et al.   BIOLOGICALS

Synthesis and assembly of a full-length human monoclonal antibody in algal chloroplasts

(2009) Miller Tran et al.   BIOTECHNOLOGY AND BIOENGINEERING

RNA Interference Silencing of a Major Lipid Droplet Protein Affects Lipid Droplet Size inChlamydomonas reinhardtii

(2009) Eric R. Moellering et al.   EUKARYOTIC CELL

TheARG9Gene Encodes the Plastid-ResidentN-Acetyl Ornithine Aminotransferase in the Green AlgaChlamydomonas reinhardtii

(2009) Claire Remacle et al.   EUKARYOTIC CELL

Heat-stable oral alga-based vaccine protects mice from Staphylococcus aureus infection

(2009) Imke A.J. Dreesen et al.   JOURNAL OF BIOTECHNOLOGY

E-CAI: a novel server to estimate an expected value of Codon Adaptation Index (eCAI)

(2008) Pere Puigbò et al.   BMC BIOINFORMATICS

Inhibition of pds Gene Expression via the RNA Interference Approach in Dunaliella salina (Chlorophyta)

(2008) Guohua Sun et al.   MARINE BIOTECHNOLOGY