Advanced genetic tools enable synthetic biology in the oleaginous microalgae Nannochloropsis sp.

PtAUREO1a and PtAUREO1b knockout mutants of the diatom Phaeodactylum tricornutum are blocked in photoacclimation to blue light

(2017) Marcus Mann et al.   JOURNAL OF PLANT PHYSIOLOGY

Producing Designer Oils in Industrial Microalgae by Rational Modulation of Co-evolving Type-2 Diacylglycerol Acyltransferases

(2017) Yi Xin et al.   Molecular Plant

Lipid production in Nannochloropsis gaditana is doubled by decreasing expression of a single transcriptional regulator

(2017) Imad Ajjawi et al.   NATURE BIOTECHNOLOGY

Evidence of the supercomplex organization of photosystem II and light-harvesting complexes in Nannochloropsis granulata

(2017) Ikumi Umetani et al.   PHOTOSYNTHESIS RESEARCH

Nitrate Reductase Knockout Uncouples Nitrate Transport from Nitrate Assimilation and Drives Repartitioning of Carbon Flux in a Model Pennate Diatom

(2017) James K. McCarthy et al.   PLANT CELL

A multi-purpose toolkit to enable advanced genome engineering in plants

(2017) Tomas Cermak et al.   PLANT CELL

RNAi-based targeted gene knockdown in the model oleaginous microalgae Nannochloropsis oceanica

(2017) Li Wei et al.   PLANT JOURNAL

Differently localized lysophosphatidic acid acyltransferases crucial for triacylglycerol biosynthesis in the oleaginous alga Nannochloropsis

(2017) Takashi Nobusawa et al.   PLANT JOURNAL

Mechanisms of Phosphorus Acquisition and Lipid Class Remodeling under P Limitation in a Marine Microalga

(2017) Alice Mühlroth et al.   PLANT PHYSIOLOGY

A Plant Cryptochrome Controls Key Features of the Chlamydomonas Circadian Clock and Its Life Cycle

(2017) Nico Müller et al.   PLANT PHYSIOLOGY

An Animal-Like Cryptochrome Controls the Chlamydomonas Sexual Cycle

(2017) Yong Zou et al.   PLANT PHYSIOLOGY

The carbonic anhydrase CAH1 is an essential component of the carbon-concentrating mechanism inNannochloropsis oceanica

(2017) Christopher W. Gee et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Diatom centromeres suggest a mechanism for nuclear DNA acquisition

(2017) Rachel E. Diner et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Enhancing photosynthetic biomass productivity of industrial oleaginous microalgae by overexpression of RuBisCO activase

(2017) Li Wei et al.   Algal Research-Biomass Biofuels and Bioproducts

Review of the algal biology program within the National Alliance for Advanced Biofuels and Bioproducts

(2017) Clifford J. Unkefer et al.   Algal Research-Biomass Biofuels and Bioproducts

Effects of chrysolaminarin synthase knockdown in the diatom Thalassiosira pseudonana : Implications of reduced carbohydrate storage relative to green algae

(2017) Mark Hildebrand et al.   Algal Research-Biomass Biofuels and Bioproducts

Verification of mutagen function of Zeocin in Nannochloropsis oceanica through transcriptome analysis

(2017) Genmei Lin et al.   Journal of Ocean University of China

An episomal vector-based CRISPR/Cas9 system for highly efficient gene knockout in human pluripotent stem cells

(2017) Yifang Xie et al.   Scientific Reports

RNAi-mediated silencing of a pyruvate dehydrogenase kinase enhances triacylglycerol biosynthesis in the oleaginous marine alga Nannochloropsis salina

(2017) Xiaonian Ma et al.   Scientific Reports

Transcription factors in microalgae: genome-wide prediction and comparative analysis

(2016) Stanislas Thiriet-Rupert et al.   BMC GENOMICS

A type 2 diacylglycerol acyltransferase accelerates the triacylglycerol biosynthesis in heterokont oleaginous microalga Nannochloropsis oceanica

(2016) Da-Wei Li et al.   JOURNAL OF BIOTECHNOLOGY

High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects

(2016) Benjamin P. Kleinstiver et al.   NATURE

Conservation of core complex subunits shaped the structure and function of photosystem I in the secondary endosymbiont algaNannochloropsis gaditana

(2016) Alessandro Alboresi et al.   NEW PHYTOLOGIST

An Indexed, Mapped Mutant Library Enables Reverse Genetics Studies of Biological Processes in Chlamydomonas reinhardtii

(2016) Xiaobo Li et al.   PLANT CELL

Diatom Phytochromes Reveal the Existence of Far-Red-Light-Based Sensing in the Ocean

(2016) Antonio Emidio Fortunato et al.   PLANT CELL

Genome editing of model oleaginous microalgaeNannochloropsisspp. by CRISPR/Cas9

(2016) Qintao Wang et al.   PLANT JOURNAL

A Palmitic Acid Elongase Affects Eicosapentaenoic Acid and Plastidial Monogalactosyldiacylglycerol Levels in Nannochloropsis

(2016) Lina-Juana Dolch et al.   PLANT PHYSIOLOGY

Five colour variants of bright luminescent protein for real-time multicolour bioimaging

(2016) Kazushi Suzuki et al.   Nature Communications

DNA-free two-gene knockout in Chlamydomonas reinhardtii via CRISPR-Cas9 ribonucleoproteins

(2016) Kwangryul Baek et al.   Scientific Reports

A CRISPR/Cas9 system adapted for gene editing in marine algae

(2016) Marianne Nymark et al.   Scientific Reports

Growth dependent silencing and resetting of DGA1 transgene in Nannochloropsis salina

(2016) Tracey A. Beacham et al.   Algal Research-Biomass Biofuels and Bioproducts

A vector for multiple gene co-expression in Chlamydomonas reinhardtii

(2016) Bin Jia et al.   Algal Research-Biomass Biofuels and Bioproducts

Genetic transformation of Nannochloropsis oculata with a bacterial phleomycin resistance gene as dominant selective marker

(2016) Xiaolei Ma et al.   Journal of Ocean University of China

Identification of lipid and saccharide constituents of whole microalgal cells by 13C solid-state NMR

(2015) Alexandre A. Arnold et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

The characteristics of TAG and EPA accumulation in Nannochloropsis oceanica IMET1 under different nitrogen supply regimes

(2015) Yingying Meng et al.   BIORESOURCE TECHNOLOGY

Effects of overexpression of a bHLH transcription factor on biomass and lipid production in Nannochloropsis salina

(2015) Nam Kyu Kang et al.   Biotechnology for Biofuels

Generation of random mutants to improve light-use efficiency of Nannochloropsis gaditana cultures for biofuel production

(2015) Giorgio Perin et al.   Biotechnology for Biofuels

Genetic improvement of the microalga Phaeodactylum tricornutum for boosting neutral lipid accumulation

(2015) Jiao Xue et al.   METABOLIC ENGINEERING

The phylogeny of the genusNannochloropsis(Monodopsidaceae, Eustigmatophyceae), with descriptions ofN. australis sp. nov. andMicrochloropsis gen. nov.

(2015) Marvin W. Fawley et al.   PHYCOLOGIA

Improved and versatile viral 2A platforms for dependable and inducible high-level expression of dicistronic nuclear genes in Chlamydomonas reinhardtii

(2015) Thomas M. Plucinak et al.   PLANT JOURNAL

Transcriptional coordination of physiological responses inNannochloropsis oceanicaCCMP1779 under light/dark cycles

(2015) Eric Poliner et al.   PLANT JOURNAL

In vivo Localization Studies in the Stramenopile Alga Nannochloropsis oceanica

(2015) Daniel Moog et al.   PROTIST

Rationally engineered Cas9 nucleases with improved specificity

(2015) I. M. Slaymaker et al.   SCIENCE

Designer diatom episomes delivered by bacterial conjugation

(2015) Bogumil J. Karas et al.   Nature Communications

Simultaneous accumulation of neutral lipids and biomass in Nannochloropsis oceanica IMET1 under high light intensity and nitrogen replete conditions

(2015) Yan Xiao et al.   Algal Research-Biomass Biofuels and Bioproducts

Metabolic engineering toward enhanced LC-PUFA biosynthesis in Nannochloropsis oceanica : Overexpression of endogenous Δ12 desaturase driven by stress-inducible promoter leads to enhanced deposition of polyunsaturated fatty acids in TAG

(2015) Yuval Kaye et al.   Algal Research-Biomass Biofuels and Bioproducts

Molecular mechanisms for photosynthetic carbon partitioning into storage neutral lipids in Nannochloropsis oceanica under nitrogen-depletion conditions

(2015) Jing Jia et al.   Algal Research-Biomass Biofuels and Bioproducts

Manipulation of oil synthesis in Nannochloropsis strain NIES-2145 with a phosphorus starvation–inducible promoter from Chlamydomonas reinhardtii

(2015) Masako Iwai et al.   Frontiers in Microbiology

Purple Chromoprotein Gene Serves as a New Selection Marker for Transgenesis of the Microalga Nannochloropsis oculata

(2015) Chen-Han Shih et al.   PLoS One

High-efficiency nuclear transformation of the oleaginous marineNannochloropsisspecies using PCR product

(2014) Fengjuan Li et al.   BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY

A pangenomic analysis of the Nannochloropsis organellar genomes reveals novel genetic variations in key metabolic genes

(2014) Shawn R Starkenburg et al.   BMC GENOMICS

Ultrastructure and Composition of the Nannochloropsis gaditana Cell Wall

(2014) Matthew J. Scholz et al.   EUKARYOTIC CELL

Effects of light and circadian clock on growth and chlorophyll accumulation ofNannochloropsis gaditana

(2014) Regina Braun et al.   JOURNAL OF PHYCOLOGY

The Synthetic Biology Open Language (SBOL) provides a community standard for communicating designs in synthetic biology

(2014) Michal Galdzicki et al.   NATURE BIOTECHNOLOGY

Choreography of Transcriptomes and Lipidomes of Nannochloropsis Reveals the Mechanisms of Oil Synthesis in Microalgae

(2014) J. Li et al.   PLANT CELL

Identification of Transcription Factor Genes and Their Correlation with the High Diversity of Stramenopiles

(2014) Francisco Javier Buitrago-Flórez et al.   PLoS One

The protein Compromised Hydrolysis of Triacylglycerols 7 (CHT7) acts as a repressor of cellular quiescence in Chlamydomonas

(2014) C.-H. Tsai et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Genome engineering empowers the diatom Phaeodactylum tricornutum for biotechnology

(2014) Fayza Daboussi et al.   Nature Communications

Nannochloropsis Genomes Reveal Evolution of Microalgal Oleaginous Traits

(2014) Dongmei Wang et al.   PLoS Genetics

An overview: biomolecules from microalgae for animal feed and aquaculture

(2014) Zahira Yaakob et al.   Journal of Biological Research-Thessaloniki

Growth, lipid production and metabolic adjustments in the euryhaline eustigmatophyte Nannochloropsis oceanica CCALA 804 in response to osmotic downshift

(2013) Dipasmita Pal et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Nannochloropsis plastid and mitochondrial phylogenomes reveal organelle diversification mechanism and intragenus phylotyping strategy in microalgae

(2013) Li Wei et al.   BMC GENOMICS

Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity

(2013) F. Ann Ran et al.   CELL

The Response of Nannochloropsis gaditana to Nitrogen Starvation Includes De Novo Biosynthesis of Triacylglycerols, a Decrease of Chloroplast Galactolipids, and Reorganization of the Photosynthetic Apparatus

(2013) Diana Simionato et al.   EUKARYOTIC CELL

A transthylakoid proton gradient and inhibitors induce a non-photochemical fluorescence quenching in unicellular algaeNannochloropsissp.

(2013) Shaona Cao et al.   FEBS LETTERS

Cloning and stress-responding expression analysis of malonyl CoA-acyl carrier protein transacylase gene of Nannochloropsis gaditana

(2013) Jinhu Tian et al.   GENE

Pathways of Lipid Metabolism in Marine Algae, Co-Expression Network, Bottlenecks and Candidate Genes for Enhanced Production of EPA and DHA in Species of Chromista

(2013) Alice Mühlroth et al.   Marine Drugs

Metabolic engineering of Phaeodactylum tricornutum for the enhanced accumulation of omega-3 long chain polyunsaturated fatty acids

(2013) Mary L. Hamilton et al.   METABOLIC ENGINEERING

Production of omega-3 eicosapentaenoic acid by metabolic engineering of Yarrowia lipolytica

(2013) Zhixiong Xue et al.   NATURE BIOTECHNOLOGY

AUREOCHROME1a-Mediated Induction of the Diatom-Specific CyclindsCYC2Controls the Onset of Cell Division in Diatoms (Phaeodactylum tricornutum)

(2013) Marie J.J. Huysman et al.   PLANT CELL

Aureochrome 1a Is Involved in the Photoacclimation of the Diatom Phaeodactylum tricornutum

(2013) Benjamin Schellenberger Costa et al.   PLoS One

Genome-wide diel growth state transitions in the diatom Thalassiosira pseudonana

(2013) J. Ashworth et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Metabolic engineering of lipid catabolism increases microalgal lipid accumulation without compromising growth

(2013) E. M. Trentacoste et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Gene Transfer from Bacteria and Archaea Facilitated Evolution of an Extremophilic Eukaryote

(2013) G. Schonknecht et al.   SCIENCE

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

Designing Synthetic Biology

(2013) Christina M. Agapakis   ACS Synthetic Biology

Engineered Luciferase Reporter from a Deep Sea Shrimp Utilizing a Novel Imidazopyrazinone Substrate

(2012) Mary P. Hall et al.   ACS Chemical Biology

A novel counter-selection method for markerless genetic modification inSynechocystissp. PCC 6803

(2012) Yi Ern Cheah et al.   BIOTECHNOLOGY PROGRESS

Gene Regulation of Carbon Fixation, Storage, and Utilization in the Diatom Phaeodactylum tricornutum Acclimated to Light/Dark Cycles

(2012) M. S. Chauton et al.   PLANT PHYSIOLOGY

Draft genome sequence and genetic transformation of the oleaginous alga Nannochloropsis gaditana

(2012) Randor Radakovits et al.   Nature Communications

Genome, Functional Gene Annotation, and Nuclear Transformation of the Heterokont Oleaginous Alga Nannochloropsis oceanica CCMP1779

(2012) Astrid Vieler et al.   PLoS Genetics

Genomic insights from the oleaginous model alga Nannochloropsis gaditana

(2012) Robert E. Jinkerson et al.   Bioengineered

Cinnamic acid, coumarin and vanillin: Alternative phenolic compounds for efficient Agrobacterium-mediated transformation of the unicellular green alga, Nannochloropsis sp.

(2011) Thye-San Cha et al.   JOURNAL OF MICROBIOLOGICAL METHODS

2A peptides provide distinct solutions to driving stop-carry on translational recoding

(2011) Pamila Sharma et al.   NUCLEIC ACIDS RESEARCH

High-efficiency homologous recombination in the oil-producing alga Nannochloropsis sp.

(2011) O. Kilian et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Increased Lipid Accumulation in the Chlamydomonas reinhardtiista7-10Starchless Isoamylase Mutant and Increased Carbohydrate Synthesis in Complemented Strains

(2010) Victoria H. Work et al.   EUKARYOTIC CELL

Transcription factor families inferred from genome sequences of photosynthetic stramenopiles

(2010) Edda Rayko et al.   NEW PHYTOLOGIST

Changes in Transcript Abundance in Chlamydomonas reinhardtii following Nitrogen Deprivation Predict Diversion of Metabolism

(2010) R. Miller et al.   PLANT PHYSIOLOGY

A common red algal origin of the apicomplexan, dinoflagellate, and heterokont plastids

(2010) J. Janouskovec et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Genome-wide analysis of the diatom cell cycle unveils a novel type of cyclins involved in environmental signaling

(2010) Marie JJ Huysman et al.   GENOME BIOLOGY

Diatom PtCPF1 is a new cryptochrome/photolyase family member with DNA repair and transcription regulation activity

(2009) Sacha Coesel et al.   EMBO REPORTS

RNA Interference Silencing of a Major Lipid Droplet Protein Affects Lipid Droplet Size inChlamydomonas reinhardtii

(2009) Eric R. Moellering et al.   EUKARYOTIC CELL

Chromalveolates and the Evolution of Plastids by Secondary Endosymbiosis

(2009) PATRICK J. KEELING   JOURNAL OF EUKARYOTIC MICROBIOLOGY

Protein Targeting into Secondary Plastids

(2009) KATHRIN BOLTE et al.   JOURNAL OF EUKARYOTIC MICROBIOLOGY

Gene silencing in the marine diatom Phaeodactylum tricornutum

(2009) Valentina De Riso et al.   NUCLEIC ACIDS RESEARCH

Microalgae for oil: Strain selection, induction of lipid synthesis and outdoor mass cultivation in a low-cost photobioreactor

(2008) Liliana Rodolfi et al.   BIOTECHNOLOGY AND BIOENGINEERING

HECTAR: A method to predict subcellular targeting in heterokonts

(2008) Bernhard Gschloessl et al.   BMC BIOINFORMATICS

The TRANSFAC project as an example of framework technology that supports the analysis of genomic regulation

(2008) E. Wingender   BRIEFINGS IN BIOINFORMATICS

ISOLATION AND CHARACTERIZATION OF THE PHYTOENE DESATURASE GENE AS A POTENTIAL SELECTIVE MARKER FOR GENETIC ENGINEERING OF THE ASTAXANTHIN-PRODUCING GREEN ALGACHLORELLA ZOFINGIENSIS(CHLOROPHYTA)

(2008) Junchao Huang et al.   JOURNAL OF PHYCOLOGY

CONDITIONAL PRODUCTION OF A FUNCTIONAL FISH GROWTH HORMONE IN THE TRANSGENIC LINE OFNANNOCHLOROPSIS OCULATA(EUSTIGMATOPHYCEAE)

(2008) Hsin Liang Chen et al.   JOURNAL OF PHYCOLOGY

The Phaeodactylum genome reveals the evolutionary history of diatom genomes

(2008) Chris Bowler et al.   NATURE

Unusual Nuclear Division in Nannochloropsis oculata (Eustigmatophyceae, Heterokonta) which May Ensure Faithful Transmission of Secondary Plastids

(2008) Ryohei Murakami et al.   PROTIST