Synthetic Biology Toolkits for Metabolic Engineering of Cyanobacteria

Toolboxes for cyanobacteria: Recent advances and future direction

(2018) Tao Sun et al.   BIOTECHNOLOGY ADVANCES

Progress of CRISPR-Cas Based Genome Editing in Photosynthetic Microbes

(2018) Mihris Ibnu Saleem Naduthodi et al.   Biotechnology Journal

Photomixotrophic chemical production in cyanobacteria

(2018) Morgan M Matson et al.   CURRENT OPINION IN BIOTECHNOLOGY

Metabolic engineering tools in model cyanobacteria

(2018) Austin L. Carroll et al.   METABOLIC ENGINEERING

Temperature enhanced succinate production concurrent with increased central metabolism turnover in the cyanobacterium Synechocystis sp. PCC 6803

(2018) Tomohisa Hasunuma et al.   METABOLIC ENGINEERING

Diversion of the long-chain acyl-ACP pool in Synechocystis to fatty alcohols through CRISPRi repression of the essential phosphate acyltransferase PlsX

(2018) Danuta Kaczmarzyk et al.   METABOLIC ENGINEERING

A balanced ATP driving force module for enhancing photosynthetic biosynthesis of 3-hydroxybutyrate from CO 2

(2018) Jason T. Ku et al.   METABOLIC ENGINEERING

Exploring native genetic elements as plug-in tools for synthetic biology in the cyanobacterium Synechocystis sp. PCC 6803

(2018) Deng Liu et al.   Microbial Cell Factories

Engineered promoters enable constant gene expression at any copy number in bacteria

(2018) Thomas H Segall-Shapiro et al.   NATURE BIOTECHNOLOGY

Improving the induction fold of riboregulators for cyanobacteria

(2018) Ippei Sakamoto et al.   RNA Biology

Functional expression of an oxygen-labile nitrogenase in an oxygenic photosynthetic organism

(2018) Ryoma Tsujimoto et al.   Scientific Reports

A Rhamnose-Inducible System for Precise and Temporal Control of Gene Expression in Cyanobacteria

(2018) Ciarán L. Kelly et al.   ACS Synthetic Biology

Progress of CRISPR-Cas Based Genome Editing in Photosynthetic Microbes

(2018) Mihris Ibnu Saleem Naduthodi et al.   Biotechnology Journal

Engineering photosynthetic production of L -lysine

(2017) Travis C. Korosh et al.   METABOLIC ENGINEERING

Plant RuBisCo assembly inE. coliwith five chloroplast chaperones including BSD2

(2017) H. Aigner et al.   SCIENCE

Combinatorial metabolic engineering using an orthogonal tri-functional CRISPR system

(2017) Jiazhang Lian et al.   Nature Communications

Global metabolic rewiring for improved CO2 fixation and chemical production in cyanobacteria

(2017) Masahiro Kanno et al.   Nature Communications

Engineering a riboswitch-based genetic platform for the self-directed evolution of acid-tolerant phenotypes

(2017) Hoang Long Pham et al.   Nature Communications

Metabolic engineering of the pentose phosphate pathway for enhanced limonene production in the cyanobacterium Synechocysti s sp. PCC 6803

(2017) Po-Cheng Lin et al.   Scientific Reports

Improvement of Squalene Production from CO2 in Synechococcus elongatus PCC 7942 by Metabolic Engineering and Scalable Production in a Photobioreactor

(2017) Sun Young Choi et al.   ACS Synthetic Biology

Enabling Graded and Large-Scale Multiplex of Desired Genes Using a Dual-Mode dCas9 Activator in Saccharomyces cerevisiae

(2017) Matthew Deaner et al.   ACS Synthetic Biology

Systematic Approaches to Efficiently Produce 2,3-Butanediol in a Marine Cyanobacterium

(2017) Nicole E. Nozzi et al.   ACS Synthetic Biology

A Genetic Toolbox for Modulating the Expression of Heterologous Genes in the Cyanobacterium Synechocystis sp. PCC 6803

(2017) Bo Wang et al.   ACS Synthetic Biology

NOT Gate Genetic Circuits to Control Gene Expression in Cyanobacteria

(2017) Arnaud Taton et al.   ACS Synthetic Biology

Cyanobacteria Maintain Constant Protein Concentration despite Genome Copy-Number Variation

(2017) Xiao-yu Zheng et al.   Cell Reports

Development of SyneBrick Vectors As a Synthetic Biology Platform for Gene Expression in Synechococcus elongatus PCC 7942

(2017) Wook Jin Kim et al.   Frontiers in Plant Science

AraBAD Based Toolkit for Gene Expression and Metabolic Robustness Improvement in Synechococcus elongatus

(2017) Yi-Qi Cao et al.   Scientific Reports

The potential of Synechococcus elongatus UTEX 2973 for sugar feedstock production

(2016) Kuo Song et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

CRISPR technologies for bacterial systems: Current achievements and future directions

(2016) Kyeong Rok Choi et al.   BIOTECHNOLOGY ADVANCES

Sugar Synthesis from CO2 in Escherichia coli

(2016) Niv Antonovsky et al.   CELL

Engineering the methylerythritol phosphate pathway in cyanobacteria for photosynthetic isoprene production from CO2

(2016) Xiang Gao et al.   Energy & Environmental Science

2,3 Butanediol production in an obligate photoautotrophic cyanobacterium in dark conditions via diverse sugar consumption

(2016) Jordan T. McEwen et al.   METABOLIC ENGINEERING

CRISPR interference as a titratable, trans -acting regulatory tool for metabolic engineering in the cyanobacterium Synechococcus sp . strain PCC 7002

(2016) Gina C. Gordon et al.   METABOLIC ENGINEERING

CRISPR-Cas9 for the genome engineering of cyanobacteria and succinate production

(2016) Hung Li et al.   METABOLIC ENGINEERING

CRISPR/Cas9 mediated targeted mutagenesis of the fast growing cyanobacterium Synechococcus elongatus UTEX 2973

(2016) Kristen E. Wendt et al.   Microbial Cell Factories

CRISPR interference (CRISPRi) for gene regulation and succinate production in cyanobacterium S. elongatus PCC 7942

(2016) Chun-Hung Huang et al.   Microbial Cell Factories

Genetic tools for advancement of Synechococcus sp. PCC 7002 as a cyanobacterial chassis

(2016) Anne M. Ruffing et al.   Microbial Cell Factories

Self-replicating shuttle vectors based on pANS, a small endogenous plasmid of the unicellular cyanobacterium Synechococcus elongatus PCC 7942

(2016) You Chen et al.   MICROBIOLOGY-SGM

The ploidy level of Synechocystis sp. PCC 6803 is highly variable and is influenced by growth phase and by chemical and physical external parameters

(2016) Karolin Zerulla et al.   MICROBIOLOGY-SGM

Creating Single-Copy Genetic Circuits

(2016) Jeong Wook Lee et al.   MOLECULAR CELL

Recycling Carbon Dioxide during Xylose Fermentation by Engineered Saccharomyces cerevisiae

(2016) Peng-Fei Xia et al.   ACS Synthetic Biology

Engineering an Obligate Photoautotrophic Cyanobacterium to Utilize Glycerol for Growth and Chemical Production

(2016) Masahiro Kanno et al.   ACS Synthetic Biology

Designing Synthetic Flexible Gene Regulation Networks Using RNA Devices in Cyanobacteria

(2016) Akiyoshi Higo et al.   ACS Synthetic Biology

A Two-Layer Gene Circuit for Decoupling Cell Growth from Metabolite Production

(2016) Tat-Ming Lo et al.   Cell Systems

Cpf1 Is A Versatile Tool for CRISPR Genome Editing Across Diverse Species of Cyanobacteria

(2016) Justin Ungerer et al.   Scientific Reports

Coupling the CRISPR/Cas9 System with Lambda Red Recombineering Enables Simplified Chromosomal Gene Replacement in Escherichia coli

(2015) Michael E. Pyne et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Construction of new synthetic biology tools for the control of gene expression in the cyanobacteriumSynechococcussp. strain PCC 7002

(2015) Erin K. Zess et al.   BIOTECHNOLOGY AND BIOENGINEERING

Oxygen-responsive genetic circuits constructed inSynechocystissp. PCC 6803

(2015) Cheryl M. Immethun et al.   BIOTECHNOLOGY AND BIOENGINEERING

Circadian Rhythms in Cyanobacteria

(2015) Susan E. Cohen et al.   MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS

Beyond editing: repurposing CRISPR–Cas9 for precision genome regulation and interrogation

(2015) Antonia A. Dominguez et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Metabolic engineering of cyanobacteria for the synthesis of commodity products

(2015) S. Andreas Angermayr et al.   TRENDS IN BIOTECHNOLOGY

Multiple Gene Repression in Cyanobacteria Using CRISPRi

(2015) Lun Yao et al.   ACS Synthetic Biology

Genome Engineering in Cyanobacteria: Where We Are and Where We Need To Go

(2015) C. Josh Ramey et al.   ACS Synthetic Biology

Controlling the Cyanobacterial Clock by Synthetically Rewiring Metabolism

(2015) Gopal K. Pattanayak et al.   Cell Reports

Scaffold-fused riboregulators for enhanced gene activation inSynechocystissp. PCC 6803

(2015) Yuta Sakai et al.   MicrobiologyOpen

Regulation of Gene Expression in Diverse Cyanobacterial Species by Using Theophylline-Responsive Riboswitches

(2014) Amy T. Ma et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Exploring metabolic engineering design principles for the photosynthetic production of lactic acid by Synechocystis sp. PCC6803

(2014) S Angermayr et al.   Biotechnology for Biofuels

A CRISPR/Cas9 toolkit for multiplex genome editing in plants

(2014) Hui-Li Xing et al.   BMC PLANT BIOLOGY

Introduction of a Synthetic CO2-fixing Photorespiratory Bypass into a Cyanobacterium

(2014) Patrick M. Shih et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Principles of genetic circuit design

(2014) Jennifer A N Brophy et al.   NATURE METHODS

Broad-host-range vector system for synthetic biology and biotechnology in cyanobacteria

(2014) Arnaud Taton et al.   NUCLEIC ACIDS RESEARCH

Application of the FLP/FRT recombination system in cyanobacteria for construction of markerless mutants

(2013) Xiaoming Tan et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Engineering cyanobacteria to improve photosynthetic production of alka(e)nes

(2013) Weihua Wang et al.   Biotechnology for Biofuels

Design of riboregulators for control of cyanobacterial (Synechocystis) protein expression

(2013) Koichi Abe et al.   BIOTECHNOLOGY LETTERS

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

(2013) Lei S. Qi et al.   CELL

RNA-guided editing of bacterial genomes using CRISPR-Cas systems

(2013) Wenyan Jiang et al.   NATURE BIOTECHNOLOGY

Theophylline-Dependent Riboswitch as a Novel Genetic Tool for Strict Regulation of Protein Expression in Cyanobacterium Synechococcus elongatus PCC 7942

(2013) Yoichi Nakahira et al.   PLANT AND CELL PHYSIOLOGY

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

Synthetic biology of cyanobacteria: unique challenges and opportunities

(2013) Bertram M. Berla et al.   Frontiers in Microbiology

Engineering Synechococcus elongatus PCC 7942 for Continuous Growth under Diurnal Conditions

(2012) Jordan T. McEwen et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

A novel counter-selection method for markerless genetic modification inSynechocystissp. PCC 6803

(2012) Yi Ern Cheah et al.   BIOTECHNOLOGY PROGRESS

Photosynthetic production of ethanol from carbon dioxide in genetically engineered cyanobacteria

(2012) Zhengxu Gao et al.   Energy & Environmental Science

ATP drives direct photosynthetic production of 1-butanol in cyanobacteria

(2012) E. I. Lan et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

An Early-Branching Microbialite Cyanobacterium Forms Intracellular Carbonates

(2012) E. Couradeau et al.   SCIENCE

Fatty acid production in genetically modified cyanobacteria

(2011) X. Liu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Design and characterization of molecular tools for a Synthetic Biology approach towards developing cyanobacterial biotechnology

(2010) Hsin-Ho Huang et al.   NUCLEIC ACIDS RESEARCH

Direct photosynthetic recycling of carbon dioxide to isobutyraldehyde

(2009) Shota Atsumi et al.   NATURE BIOTECHNOLOGY

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

DNA assembler, an in vivo genetic method for rapid construction of biochemical pathways

(2008) Zengyi Shao et al.   NUCLEIC ACIDS RESEARCH