Progress of CRISPR-Cas Based Genome Editing in Photosynthetic Microbes

Multiplex Gene Editing in Rice Using the CRISPR-Cpf1 System

(2017) Mugui Wang et al.   Molecular Plant

Lipid production in Nannochloropsis gaditana is doubled by decreasing expression of a single transcriptional regulator

(2017) Imad Ajjawi et al.   NATURE BIOTECHNOLOGY

Application of CRISPR Interference for Metabolic Engineering of the Heterocyst-Forming Multicellular Cyanobacterium Anabaena sp. PCC 7120

(2017) Akiyoshi Higo et al.   PLANT AND CELL PHYSIOLOGY

New variants of CRISPR RNA-guided genome editing enzymes

(2017) Jana Murovec et al.   PLANT BIOTECHNOLOGY JOURNAL

Efficient targeted DNA editing and replacement inChlamydomonas reinhardtiiusing Cpf1 ribonucleoproteins and single-stranded DNA

(2017) Aron Ferenczi et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A thermostable Cas9 with increased lifetime in human plasma

(2017) Lucas B. Harrington et al.   Nature Communications

CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing

(2017) Miguel A. Moreno-Mateos et al.   Nature Communications

A gene-within-a-gene Cas9/sgRNA hybrid construct enables gene editing and gene replacement strategies in Chlamydomonas reinhardtii

(2017) Wen Zhi Jiang et al.   Algal Research-Biomass Biofuels and Bioproducts

Comparative genomic analysis of retrogene repertoire in two green algae Volvox carteri and Chlamydomonas reinhardtii

(2016) Marcin Jąkalski et al.   Biology Direct

CRISPR interference as a titratable, trans -acting regulatory tool for metabolic engineering in the cyanobacterium Synechococcus sp . strain PCC 7002

(2016) Gina C. Gordon et al.   METABOLIC ENGINEERING

CRISPR-Cas9 for the genome engineering of cyanobacteria and succinate production

(2016) Hung Li et al.   METABOLIC ENGINEERING

CRISPR/Cas9 mediated targeted mutagenesis of the fast growing cyanobacterium Synechococcus elongatus UTEX 2973

(2016) Kristen E. Wendt et al.   Microbial Cell Factories

CRISPR interference (CRISPRi) for gene regulation and succinate production in cyanobacterium S. elongatus PCC 7942

(2016) Chun-Hung Huang et al.   Microbial Cell Factories

The ploidy level of Synechocystis sp. PCC 6803 is highly variable and is influenced by growth phase and by chemical and physical external parameters

(2016) Karolin Zerulla et al.   MICROBIOLOGY-SGM

The CRISPR-associated DNA-cleaving enzyme Cpf1 also processes precursor CRISPR RNA

(2016) Ines Fonfara et al.   NATURE

Multiplex gene editing by CRISPR–Cpf1 using a single crRNA array

(2016) Bernd Zetsche et al.   NATURE BIOTECHNOLOGY

Genome editing of model oleaginous microalgaeNannochloropsisspp. by CRISPR/Cas9

(2016) Qintao Wang et al.   PLANT JOURNAL

Editing of the urease gene by CRISPR-Cas in the diatom Thalassiosira pseudonana

(2016) Amanda Hopes et al.   Plant Methods

Diverse evolutionary roots and mechanistic variations of the CRISPR-Cas systems

(2016) Prarthana Mohanraju et al.   SCIENCE

DNA-free two-gene knockout in Chlamydomonas reinhardtii via CRISPR-Cas9 ribonucleoproteins

(2016) Kwangryul Baek et al.   Scientific Reports

A CRISPR/Cas9 system adapted for gene editing in marine algae

(2016) Marianne Nymark et al.   Scientific Reports

CRISPR/Cas9-induced knockout and knock-in mutations in Chlamydomonas reinhardtii

(2016) Sung-Eun Shin et al.   Scientific Reports

Efficient targeted mutagenesis of rice and tobacco genomes using Cpf1 from Francisella novicida

(2016) Akira Endo et al.   Scientific Reports

Cpf1 Is A Versatile Tool for CRISPR Genome Editing Across Diverse Species of Cyanobacteria

(2016) Justin Ungerer et al.   Scientific Reports

Targeted genome modifications in soybean with CRISPR/Cas9

(2015) Thomas B Jacobs et al.   BMC BIOTECHNOLOGY

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

(2015) Bernd Zetsche et al.   CELL

Multiple Gene Repression in Cyanobacteria Using CRISPRi

(2015) Lun Yao et al.   ACS Synthetic Biology

Harnessing CRISPR-Cas9 immunity for genetic engineering

(2014) Emmanuelle Charpentier et al.   CURRENT OPINION IN MICROBIOLOGY

Successful Transient Expression of Cas9 and Single Guide RNA Genes in Chlamydomonas reinhardtii

(2014) Wenzhi Jiang et al.   EUKARYOTIC CELL

Nannochloropsis Genomes Reveal Evolution of Microalgal Oleaginous Traits

(2014) Dongmei Wang et al.   PLoS Genetics

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

(2013) Lei S. Qi et al.   CELL

Chromosome Scale Genome Assembly and Transcriptome Profiling of Nannochloropsis gaditana in Nitrogen Depletion

(2013) Elisa Corteggiani Carpinelli et al.   Molecular Plant

Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease

(2013) Vladimir Nekrasov et al.   NATURE BIOTECHNOLOGY

RNA-guided editing of bacterial genomes using CRISPR-Cas systems

(2013) Wenyan Jiang et al.   NATURE BIOTECHNOLOGY

CRISPR interference (CRISPRi) for sequence-specific control of gene expression

(2013) Matthew H Larson et al.   Nature Protocols

RNA-Guided Human Genome Engineering via Cas9

(2013) P. Mali et al.   SCIENCE

Nuclear gene targeting inChlamydomonasusing engineered zinc-finger nucleases

(2012) Irina Sizova et al.   PLANT JOURNAL

Robust Expression and Secretion of Xylanase1 in Chlamydomonas reinhardtii by Fusion to a Selection Gene and Processing with the FMDV 2A Peptide

(2012) Beth A. Rasala et al.   PLoS One

NUCLEAR MONOPLOIDY AND ASEXUAL PROPAGATION OF NANNOCHLOROPSIS OCEANICA (EUSTIGMATOPHYCEAE) AS REVEALED BY ITS GENOME SEQUENCE1

(2011) Kehou Pan et al.   JOURNAL OF PHYCOLOGY

Microalgae for the production of bulk chemicals and biofuels

(2010) Rene H Wijffels et al.   Biofuels Bioproducts & Biorefining-Biofpr

Diatoms in biotechnology: modern tools and applications

(2008) Andrew Bozarth et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

CO2 bio-mitigation using microalgae

(2008) Bei Wang et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY