Beyond antibody engineering: directed evolution of alternative binding scaffolds and enzymes using yeast surface display

Targeting Insulin Receptor in Breast Cancer Using Small Engineered Protein Scaffolds

(2017) Jie Ying Chan et al.   MOLECULAR CANCER THERAPEUTICS

Monobodies and other synthetic binding proteins for expanding protein science

(2017) Fern Sha et al.   PROTEIN SCIENCE

Semi-synthetic vNAR libraries screened against therapeutic antibodies primarily deliver anti-idiotypic binders

(2017) Doreen Könning et al.   Scientific Reports

In vitro-engineered non-antibody protein therapeutics

(2017) Rudo Simeon et al.   Protein & Cell

An engineered protein antagonist of K-Ras/B-Raf interaction

(2017) Monique J. Kauke et al.   Scientific Reports

Engineered knottin peptides as diagnostics, therapeutics, and drug delivery vehicles

(2016) James R Kintzing et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

A Camelid-derived Antibody Fragment Targeting the Active Site of a Serine Protease Balances between Inhibitor and Substrate Behavior

(2016) Tobias Kromann-Hansen et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Strong Enrichment of Aromatic Residues in Binding Sites from a Charge-neutralized Hyperthermostable Sso7d Scaffold Library

(2016) Michael W. Traxlmayr et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Generation of Fluorogen-Activating Designed Ankyrin Repeat Proteins (FADAs) as Versatile Sensor Tools

(2016) Marco Schütz et al.   JOURNAL OF MOLECULAR BIOLOGY

Transpo-mAb display: Transposition-mediated B cell display and functional screening of full-length IgG antibody libraries

(2016) Lorenz Waldmeier et al.   mAbs

Isolation of a pH-Sensitive IgNAR Variable Domain from a Yeast-Displayed, Histidine-Doped Master Library

(2016) Doreen Könning et al.   MARINE BIOTECHNOLOGY

64Cu-Labeled Gp2 Domain for PET Imaging of Epidermal Growth Factor Receptor

(2016) Max A. Kruziki et al.   MOLECULAR PHARMACEUTICS

Low-stringency selection of TEM1 for BLIP shows interface plasticity and selection for faster binders

(2016) Ruth Cohen-Khait et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Expanding the Enzyme Universe: Accessing Non-Natural Reactions by Mechanism-Guided Directed Evolution

(2015) Hans Renata et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Designed Ankyrin Repeat Proteins (DARPins): Binding Proteins for Research, Diagnostics, and Therapy

(2015) Andreas Plückthun   Annual Review of Pharmacology and Toxicology

Selection of specific interactors from phage display library based on sea lamprey variable lymphocyte receptor sequences

(2015) Magdalena Wezner-Ptasinska et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

A 45-Amino-Acid Scaffold Mined from the PDB for High-Affinity Ligand Engineering

(2015) Max A. Kruziki et al.   CHEMISTRY & BIOLOGY

Cystine-knot peptides targeting cancer-relevant human cytotoxic T lymphocyte-associated antigen 4 (CTLA-4)

(2015) Franziska Maaß et al.   JOURNAL OF PEPTIDE SCIENCE

The Shark Strikes Twice: Hypervariable Loop 2 of Shark IgNAR Antibody Variable Domains and Its Potential to Function as an Autonomous Paratope

(2015) Stefan Zielonka et al.   MARINE BIOTECHNOLOGY

High-throughput analysis and protein engineering using microcapillary arrays

(2015) Bob Chen et al.   Nature Chemical Biology

Isolation and Characterization of a Monobody with a Fibronectin Domain III Scaffold That Specifically Binds EphA2

(2015) Seung-Hwan Park et al.   PLoS One

The nature of chemical innovation: new enzymes by evolution

(2015) Frances H. Arnold   QUARTERLY REVIEWS OF BIOPHYSICS

Engineering pH responsive fibronectin domains for biomedical applications

(2015) Pete Heinzelman et al.   Journal of Biological Engineering

Construction of pH-sensitive Her2-binding IgG1-Fc by directed evolution

(2014) Michael W. Traxlmayr et al.   Biotechnology Journal

Ultra-High-Throughput Screening Method for the Directed Evolution of Glucose Oxidase

(2014) Raluca Ostafe et al.   CHEMISTRY & BIOLOGY

Engineered proteins with desired specificity: DARPins, other alternative scaffolds and bispecific IgGs

(2014) Christian Jost et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Shark variable new antigen receptor biologics – a novel technology platform for therapeutic drug development

(2014) Marina Kovaleva et al.   EXPERT OPINION ON BIOLOGICAL THERAPY

High Throughput Screening and Selection Methods for Directed Enzyme Evolution

(2014) Han Xiao et al.   INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH

Shark Attack: High affinity binding proteins derived from shark vNAR domains by stepwise in vitro affinity maturation

(2014) Stefan Zielonka et al.   JOURNAL OF BIOTECHNOLOGY

Construction of a synthetic phage-displayed Nanobody library with CDR3 regions randomized by trinucleotide cassettes for diagnostic applications

(2014) Junrong Yan et al.   Journal of Translational Medicine

Structural insights and biomedical potential of IgNAR scaffolds from sharks

(2014) Stefan Zielonka et al.   mAbs

A generic approach to engineer antibody pH-switches using combinatorial histidine scanning libraries and yeast display

(2014) Christian Schröter et al.   mAbs

Computational design of a pH-sensitive IgG binding protein

(2014) E.-M. Strauch et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Directed Evolution of a Probe Ligase with Activity in the Secretory Pathway and Application to Imaging Intercellular Protein–Protein Interactions

(2013) Katharine A. White et al.   BIOCHEMISTRY

Engineering the Substrate Specificity of the DhbE Adenylation Domain by Yeast Cell Surface Display

(2013) Keya Zhang et al.   CHEMISTRY & BIOLOGY

Therapeutic antibody engineering by high efficiency cell screening

(2013) Achim Doerner et al.   FEBS LETTERS

Llama-derived Single Variable Domains (Nanobodies) Directed against Chemokine Receptor CXCR7 Reduce Head and Neck Cancer Cell Growthin Vivo

(2013) David Maussang et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Retrocyte Display® technology: Generation and screening of a high diversity cellular antibody library

(2013) Ekaterina Breous-Nystrom et al.   METHODS

Combinatorial Optimization of Cystine-Knot Peptides towards High-Affinity Inhibitors of Human Matriptase-1

(2013) Bernhard Glotzbach et al.   PLoS One

Engineering of TEV protease variants by yeast ER sequestration screening (YESS) of combinatorial libraries

(2013) L. Yi et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Dissection of the BCR-ABL signaling network using highly specific monobody inhibitors to the SHP2 SH2 domains

(2013) F. Sha et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Epitope-Guided Engineering of Monobody Binders for in Vivo Inhibition of Erk-2 Signaling

(2012) Jasdeep K. Mann et al.   ACS Chemical Biology

Sugar-Binding Proteins from Fish: Selection of High Affinity “Lambodies” That Recognize Biomedically Relevant Glycans

(2012) Xia Hong et al.   ACS Chemical Biology

Recent developments in yeast cell surface display toward extended applications in biotechnology

(2012) Tsutomu Tanaka et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Engineering antibodies by yeast display

(2012) Eric T. Boder et al.   ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS

Directed evolution of stabilized IgG1-Fc scaffolds by application of strong heat shock to libraries displayed on yeast

(2012) Michael W. Traxlmayr et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Protein selection using yeast surface display

(2012) Nimish Gera et al.   METHODS

Design of pH Sensitive Binding Proteins from the Hyperthermophilic Sso7d Scaffold

(2012) Nimish Gera et al.   PLoS One

Design of a binding scaffold based on variable lymphocyte receptors of jawless vertebrates by module engineering

(2012) S.-C. Lee et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Combination of site-directed mutagenesis and yeast surface display enhances Rhizomucor miehei lipase esterification activity in organic solvent

(2011) Shuang-yan Han et al.   BIOTECHNOLOGY LETTERS

Generation of Bivalent and Bispecific Kringle Single Domains by Loop Grafting as Potent Agonists against Death Receptors 4 and 5

(2011) Chang-Han Lee et al.   JOURNAL OF MOLECULAR BIOLOGY

Highly Stable Binding Proteins Derived from the Hyperthermophilic Sso7d Scaffold

(2011) Nimish Gera et al.   JOURNAL OF MOLECULAR BIOLOGY

Teaching an Old Scaffold New Tricks: Monobodies Constructed Using Alternative Surfaces of the FN3 Scaffold

(2011) Akiko Koide et al.   JOURNAL OF MOLECULAR BIOLOGY

Miniproteins as Phage Display-Scaffolds for Clinical Applications

(2011) Frederic Zoller et al.   MOLECULES

Functional Mutation of Multiple Solvent-Exposed Loops in the Ecballium elaterium Trypsin Inhibitor-II Cystine Knot Miniprotein

(2011) Richard H. Kimura et al.   PLoS One

A general strategy for the evolution of bond-forming enzymes using yeast display

(2011) I. Chen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Epidermal growth factor receptor downregulation by small heterodimeric binding proteins

(2011) Benjamin J. Hackel et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Cystine-knot peptides engineered with specificities for αIIbβ3 or αIIbβ3 and αvβ3 integrins are potent inhibitors of platelet aggregation

(2010) Adam P. Silverman et al.   JOURNAL OF MOLECULAR RECOGNITION

Antibody recycling by engineered pH-dependent antigen binding improves the duration of antigen neutralization

(2010) Tomoyuki Igawa et al.   NATURE BIOTECHNOLOGY

A potent and highly specific FN3 monobody inhibitor of the Abl SH2 domain

(2010) John Wojcik et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Engineering of a human kringle domain into agonistic and antagonistic binding proteins functioning in vitro and in vivo

(2010) C.-H. Lee et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

CXCR4 nanobodies (VHH-based single variable domains) potently inhibit chemotaxis and HIV-1 replication and mobilize stem cells

(2010) S. Jahnichen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

T7 phage protein Gp2 inhibits the Escherichia coli RNA polymerase by antagonizing stable DNA strand separation near the transcription start site

(2010) B. Camara et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

An improved yeast transformation method for the generation of very large human antibody libraries

(2010) Lorenzo Benatuil et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Decorating microbes: surface display of proteins on Escherichia coli

(2010) Edwin van Bloois et al.   TRENDS IN BIOTECHNOLOGY

Safety and efficacy of intrathecal ziconotide in the management of severe chronic pain

(2010) Howard Howard Smith   Therapeutics and Clinical Risk Management

Ultrahigh-Throughput FACS-Based Screening for Directed Enzyme Evolution

(2009) Guangyu Yang et al.   CHEMBIOCHEM

FN3: a new protein scaffold reaches the clinic

(2009) Laird Bloom et al.   DRUG DISCOVERY TODAY

Engineered affinity proteins—Generation and applications

(2009) Caroline Grönwall et al.   JOURNAL OF BIOTECHNOLOGY

Isolation of antigen-binding camelid heavy chain antibody fragments (nanobodies) from an immune library displayed on the surface of Pichia pastoris

(2009) Stefan Ryckaert et al.   JOURNAL OF BIOTECHNOLOGY

Exploring protein fitness landscapes by directed evolution

(2009) Philip A. Romero et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

High-affinity lamprey VLRA and VLRB monoclonal antibodies

(2009) S. Tasumi et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Immunological applications of single-domain llama recombinant antibodies isolated from a naïve library

(2009) Ana Monegal et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Engineered cystine knot peptides that bind αvβ3, αvβ5, and α5β1 integrins with low-nanomolar affinity

(2009) Richard H. Kimura et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

Interrogating and Predicting Tolerated Sequence Diversity in Protein Folds: Application to E. elaterium Trypsin Inhibitor-II Cystine-Knot Miniprotein

(2009) Jennifer L. Lahti et al.   PLoS Computational Biology

A Decade of Yeast Surface Display Technology: Where Are We Now?

(2008) Eric Shusta et al.   COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING

Alternative binding proteins: Biological activity and therapeutic potential of cystine-knot miniproteins

(2008) Harald Kolmar   FEBS Journal

Alternative binding proteins: Anticalins - harnessing the structural plasticity of the lipocalin ligand pocket to engineer novel binding activities

(2008) Arne Skerra   FEBS Journal

General Strategy to Humanize a Camelid Single-domain Antibody and Identification of a Universal Humanized Nanobody Scaffold

(2008) Cécile Vincke et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Picomolar Affinity Fibronectin Domains Engineered Utilizing Loop Length Diversity, Recursive Mutagenesis, and Loop Shuffling

(2008) Benjamin J. Hackel et al.   JOURNAL OF MOLECULAR BIOLOGY

Efficient Selection of DARPins with Sub-nanomolar Affinities using SRP Phage Display

(2008) Daniel Steiner et al.   JOURNAL OF MOLECULAR BIOLOGY

Engineered Cystine-Knot Peptides that Bind αvβ3 Integrin with Antibody-Like Affinities

(2008) Adam P. Silverman et al.   JOURNAL OF MOLECULAR BIOLOGY

Highly L and D enantioselective variants of horseradish peroxidase discovered by an ultrahigh-throughput selection method

(2008) E. Antipov et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structure and specificity of lamprey monoclonal antibodies

(2008) B. R. Herrin et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA