Expanding the Enzyme Universe: Accessing Non-Natural Reactions by Mechanism-Guided Directed Evolution

Improved Cyclopropanation Activity of Histidine-Ligated Cytochrome P450 Enables the Enantioselective Formal Synthesis of Levomilnacipran

(2014) Z. Jane Wang et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Intramolecular C(sp3)H amination of arylsulfonyl azides with engineered and artificial myoglobin-based catalysts

(2014) Melanie Bordeaux et al.   BIOORGANIC & MEDICINAL CHEMISTRY

Non-natural Olefin Cyclopropanation Catalyzed by Diverse Cytochrome P450s and Other Hemoproteins

(2014) Thomas Heel et al.   CHEMBIOCHEM

Expanding P450 catalytic reaction space through evolution and engineering

(2014) John A McIntosh et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Artificial metalloenzymes for enantioselective catalysis

(2014) Jeffrey Bos et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Recent achievments in the design and engineering of artificial metalloenzymes

(2014) Marc Dürrenberger et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Connectivity between Catalytic Landscapes of the Metallo-β-Lactamase Superfamily

(2014) Florian Baier et al.   JOURNAL OF MOLECULAR BIOLOGY

Enzyme-Controlled Nitrogen-Atom Transfer Enables Regiodivergent C–H Amination

(2014) Todd K. Hyster et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Enantioselective Imidation of Sulfides via Enzyme-Catalyzed Intermolecular Nitrogen-Atom Transfer

(2014) Christopher C. Farwell et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Direct nitration and azidation of aliphatic carbons by an iron-dependent halogenase

(2014) Megan L Matthews et al.   Nature Chemical Biology

P450-Catalyzed Intramolecular sp3 C–H Amination with Arylsulfonyl Azide Substrates

(2014) Ritesh Singh et al.   ACS Catalysis

P450-catalyzed asymmetric cyclopropanation of electron-deficient olefins under aerobic conditions

(2014) Hans Renata et al.   Catalysis Science & Technology

Enantioselective Intramolecular CH Amination Catalyzed by Engineered Cytochrome P450 Enzymes In Vitro and In Vivo

(2013) John A. McIntosh et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Computational Enzyme Design

(2013) Gert Kiss et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Synthesis of Heterocyclic Terpenoids by Promiscuous Squalene-Hopene Cyclases

(2013) Miriam Seitz et al.   CHEMBIOCHEM

Bis(sulfonylimide)ruthenium(VI) Porphyrins: X-ray Crystal Structure and Mechanism of CH Bond Amination by Density Functional Theory Calculations

(2013) Zhen Guo et al.   CHEMISTRY-A EUROPEAN JOURNAL

Squalene hopene cyclases: highly promiscuous and evolvable catalysts for stereoselective CC and CX bond formation

(2013) Stephan C Hammer et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

De novo enzymes by computational design

(2013) Hajo Kries et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Molecular Engineering of Organophosphate Hydrolysis Activity from a Weak Promiscuous Lactonase Template

(2013) Monika M. Meier et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Biocatalysis in Organic Chemistry and Biotechnology: Past, Present, and Future

(2013) Manfred T. Reetz   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Elucidation of the Fe(iv)=O intermediate in the catalytic cycle of the halogenase SyrB2

(2013) Shaun D. Wong et al.   NATURE

A serine-substituted P450 catalyzes highly efficient carbene transfer to olefins in vivo

(2013) Pedro S Coelho et al.   Nature Chemical Biology

Cytochrome P450-catalyzed insertion of carbenoids into N–H bonds

(2013) Z. Jane Wang et al.   Chemical Science

Prokaryotic squalene-hopene cyclases can be converted to citronellal cyclases by single amino acid exchange

(2012) Gabriele Siedenburg et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Evolution of atrazine-degrading capabilities in the environment

(2012) Nikolina Udiković-Kolić et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Reconstructing a Missing Link in the Evolution of a Recently Diverged Phosphotriesterase by Active-Site Loop Remodeling

(2012) Livnat Afriat-Jurnou et al.   BIOCHEMISTRY

Catalytic Versatility and Backups in Enzyme Active Sites: The Case of Serum Paraoxonase 1

(2012) Moshe Ben-David et al.   JOURNAL OF MOLECULAR BIOLOGY

Substrate specificity of a novel squalene-hopene cyclase from Zymomonas mobilis

(2012) Miriam Seitz et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Iridium-Catalyzed Enantioselective Polyene Cyclization

(2012) Michael A. Schafroth et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Microbial engineering for the production of advanced biofuels

(2012) Pamela P. Peralta-Yahya et al.   NATURE

Engineering the third wave of biocatalysis

(2012) U. T. Bornscheuer et al.   NATURE

Iron(III)/NaBH4-Mediated Additions to Unactivated Alkenes: Synthesis of Novel 20′-Vinblastine Analogues

(2012) Erick K. Leggans et al.   ORGANIC LETTERS

A Measure of the Promiscuity of Proteins and Characteristics of Residues in the Vicinity of the Catalytic Site That Regulate Promiscuity

(2012) Sandeep Chakraborty et al.   PLoS One

Intramolecular Epistasis and the Evolution of a New Enzymatic Function

(2012) Sajid Noor et al.   PLoS One

Robust design and optimization of retroaldol enzymes

(2012) Eric A. Althoff et al.   PROTEIN SCIENCE

Olefin Cyclopropanation via Carbene Transfer Catalyzed by Engineered Cytochrome P450 Enzymes

(2012) P. S. Coelho et al.   SCIENCE

Activation-Independent Cyclization of Monoterpenoids

(2011) Gabriele Siedenburg et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Divergence and Convergence in Enzyme Evolution: Parallel Evolution of Paraoxonases from Quorum-quenching Lactonases

(2011) Mikael Elias et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Chemo-enzymatic syntheses of drimane-type sesquiterpenes and the fundamental core of hongoquercin meroterpenoid by recombinant squalene–hopene cyclase

(2011) Yukie Yonemura et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Selective Formation of Six-Membered Oxa- and Carbocycles by the In(III)-Activated Ring Closure of Acetylenic Substrates

(2011) Wen-Wei Qiu et al.   ORGANIC LETTERS

Enzyme Promiscuity: A Mechanistic and Evolutionary Perspective

(2010) Olga Khersonsky and Dan S. Tawfik   Annual Review of Biochemistry

Biocatalysis—key to sustainable industrial chemistry

(2010) Roland Wohlgemuth   CURRENT OPINION IN BIOTECHNOLOGY

Enantioselective Thiourea-Catalyzed Cationic Polycyclizations

(2010) Robert R. Knowles et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Microbial production of fatty-acid-derived fuels and chemicals from plant biomass

(2010) Eric J. Steen et al.   NATURE

Messy biology and the origins of evolutionary innovations

(2010) Dan S Tawfik   Nature Chemical Biology

Crystallization and preliminary X-ray crystallographic analysis of squalene-hopene cyclase from Alicyclobacillus acidocaldarius

(2010) K.-Ulrich Wendt et al.   PROTEIN SCIENCE

An exciting but challenging road ahead for computational enzyme design

(2010) David Baker   PROTEIN SCIENCE

Biocatalytic Asymmetric Synthesis of Chiral Amines from Ketones Applied to Sitagliptin Manufacture

(2010) C. K. Savile et al.   SCIENCE

Catalytic Improvement and Evolution of Atrazine Chlorohydrolase

(2009) C. Scott et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Enzyme (re)design: lessons from natural evolution and computation

(2009) John A Gerlt et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Desaturases: Emerging Models for Understanding Functional Diversification of Diiron-containing Enzymes

(2009) John Shanklin et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Directed evolution drives the next generation of biocatalysts

(2009) Nicholas J Turner   Nature Chemical Biology

Development of a Biocatalytic Process as an Alternative to the (−)-DIP-Cl-Mediated Asymmetric Reduction of a Key Intermediate of Montelukast

(2009) Jack Liang et al.   ORGANIC PROCESS RESEARCH & DEVELOPMENT

Stringency of the 2-His–1-Asp Active-Site Motif in Prolyl 4-Hydroxylase

(2009) Kelly L. Gorres et al.   PLoS One

Substrate positioning controls the partition between halogenation and hydroxylation in the aliphatic halogenase, SyrB2

(2009) M. L. Matthews et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Fermentative butanol production by clostridia

(2008) Sang Yup Lee et al.   BIOTECHNOLOGY AND BIOENGINEERING

Catalytic Promiscuity of Halohydrin Dehalogenase and its Application in Enantioselective Epoxide Ring Opening

(2008) Ghannia Hasnaoui-Dijoux et al.   CHEMBIOCHEM

Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels

(2008) Shota Atsumi et al.   NATURE

De Novo Computational Design of Retro-Aldol Enzymes

(2008) L. Jiang et al.   SCIENCE

Tryptophan synthase: the workings of a channeling nanomachine

(2008) Michael F. Dunn et al.   TRENDS IN BIOCHEMICAL SCIENCES