Isolation of a pH-Sensitive IgNAR Variable Domain from a Yeast-Displayed, Histidine-Doped Master Library

 De novoisolation of antibodies with pH-dependent binding properties

(2015) Pauline Bonvin et al.   mAbs

pH-dependent antigen-binding antibodies as a novel therapeutic modality

(2014) T. Igawa et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Construction of pH-sensitive Her2-binding IgG1-Fc by directed evolution

(2014) Michael W. Traxlmayr et al.   Biotechnology Journal

Shark Attack: High affinity binding proteins derived from shark vNAR domains by stepwise in vitro affinity maturation

(2014) Stefan Zielonka et al.   JOURNAL OF BIOTECHNOLOGY

Structural insights and biomedical potential of IgNAR scaffolds from sharks

(2014) Stefan Zielonka et al.   mAbs

A generic approach to engineer antibody pH-switches using combinatorial histidine scanning libraries and yeast display

(2014) Christian Schröter et al.   mAbs

Computational design of a pH-sensitive IgG binding protein

(2014) E.-M. Strauch et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Engineered protein A ligands, derived from a histidine-scanning library, facilitate the affinity purification of IgG under mild acidic conditions

(2014) Masayuki Tsukamoto et al.   Journal of Biological Engineering

Human TNF cytokine neutralization with a vNAR fromHeterodontus franciscishark: A potential therapeutic use

(2013) Tanya Camacho-Villegas et al.   mAbs

Increasing Serum Half-life and Extending Cholesterol Loweringin Vivoby Engineering Antibody with pH-sensitive Binding to PCSK9

(2012) Javier Chaparro-Riggers et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Design of pH Sensitive Binding Proteins from the Hyperthermophilic Sso7d Scaffold

(2012) Nimish Gera et al.   PLoS One

Detection of EpCAM positive and negative circulating tumor cells in metastatic breast cancer patients

(2011) Robert Königsberg et al.   ACTA ONCOLOGICA

A combinatorial histidine scanning library approach to engineer highly pH-dependent protein switches

(2011) Megan L. Murtaugh et al.   PROTEIN SCIENCE

Two Independent Histidines, One in Human Prolactin and One in Its Receptor, Are Critical for pH-dependent Receptor Recognition and Activation

(2010) Mandar V. Kulkarni et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Antibody recycling by engineered pH-dependent antigen binding improves the duration of antigen neutralization

(2010) Tomoyuki Igawa et al.   NATURE BIOTECHNOLOGY

An improved yeast transformation method for the generation of very large human antibody libraries

(2010) Lorenzo Benatuil et al.   PROTEIN ENGINEERING DESIGN & SELECTION