Fluorescent biosensors illuminate the spatial regulation of cell signaling across scales

Fluorescent biosensor imaging meets deterministic mathematical modelling: quantitative investigation of signalling compartmentalization

(2023) Clara Posner et al.   JOURNAL OF PHYSIOLOGY-LONDON

Loss of CDK4/6 activity in S/G2 phase leads to cell cycle reversal

(2023) James A. Cornwell et al.   NATURE

Receptor-associated independent cAMP nanodomains mediate spatiotemporal specificity of GPCR signaling

(2022) Selma E. Anton et al.   CELL

Complexity of subcellular metabolism: strategies for compartment-specific profiling

(2022) Tushar H More et al.   CURRENT OPINION IN BIOTECHNOLOGY

FUCCI-Based Live Imaging Platform Reveals Cell Cycle Dynamics and Identifies Pro-proliferative Compounds in Human iPSC-Derived Cardiomyocytes

(2022) Francesca Murganti et al.   Frontiers in Cardiovascular Medicine

Spatial regulation of AMPK signaling revealed by a sensitive kinase activity reporter

(2022) Danielle L. Schmitt et al.   Nature Communications

Transcriptional heterogeneity and cell cycle regulation as central determinants of primitive endoderm priming

(2022) Marta Perera et al.   eLife

A feedback loop between lamellipodial extension and HGF-ERK signaling specifies leader cells during collective cell migration

(2022) Naoya Hino et al.   DEVELOPMENTAL CELL

Non-canonical β-adrenergic activation of ERK at endosomes

(2022) Yonghoon Kwon et al.   NATURE

Advances in measuring cancer cell metabolism with subcellular resolution

(2022) Victor Ruiz-Rodado et al.   NATURE METHODS

Functional architecture of pancreatic islets identifies a population of first responder cells that drive the first-phase calcium response

(2022) Vira Kravets et al.   PLOS BIOLOGY

ERK signaling for cell migration and invasion

(2022) Shiela C. Samson et al.   Frontiers in Molecular Biosciences

Deciphering molecular interactions by proximity labeling

(2021) Wei Qin et al.   NATURE METHODS

Noncanonical scaffolding of Gαi and β-arrestin by G protein–coupled receptors

(2021) Jeffrey S. Smith et al.   SCIENCE

Transient phases of OXPHOS inhibitor resistance reveal underlying metabolic heterogeneity in single cells

(2021) Nont Kosaisawe et al.   Cell Metabolism

Structure- and mechanism-guided design of single fluorescent protein-based biosensors

(2021) Yusuke Nasu et al.   Nature Chemical Biology

The principles of directed cell migration

(2021) Shuvasree SenGupta et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Live-imaging of endothelial Erk activity reveals dynamic and sequential signalling events during regenerative angiogenesis

(2021) Kazuhide S Okuda et al.   eLife

Metabolic Homeostasis in Life as We Know It: Its Origin and Thermodynamic Basis

(2021) David F. Wilson et al.   Frontiers in Physiology

Collective ERK/Akt activity waves orchestrate epithelial homeostasis by driving apoptosis-induced survival

(2021) Paolo Armando Gagliardi et al.   DEVELOPMENTAL CELL

DrFLINC Contextualizes Super-resolution Activity Imaging

(2021) Wei Lin et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

AMPK: restoring metabolic homeostasis over space and time

(2021) Elijah Trefts et al.   MOLECULAR CELL

Scaffolding mechanism of arrestin-2 in the cRaf/MEK1/ERK signaling cascade

(2021) Changxiu Qu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Mitochondria-localized AMPK responds to local energetics and contributes to exercise and energetic stress-induced mitophagy

(2021) Joshua C. Drake et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Spatial localisation meets biomolecular networks

(2021) Govind Menon et al.   Nature Communications

Cell Cycle Commitment and the Origins of Cell Cycle Variability

(2021) Robert F. Brooks   Frontiers in Cell and Developmental Biology

Deciphering cell signaling networks with massively multiplexed biosensor barcoding

(2021) Jr-Ming Yang et al.   CELL

Integrative RNA-omics Discovers GNAS Alternative Splicing as a Phenotypic Driver of Splicing Factor–Mutant Neoplasms

(2021) Emily C. Wheeler et al.   Cancer Discovery

A Highly Sensitive Fluorescent Akt Biosensor Reveals Lysosome-Selective Regulation of Lipid Second Messengers and Kinase Activity

(2021) Mingyuan Chen et al.   ACS Central Science

Signaling diversity enabled by Rap1-regulated plasma membrane ERK with distinct temporal dynamics

(2020) Jeremiah Keyes et al.   eLife

ERK-Mediated Mechanochemical Waves Direct Collective Cell Polarization

(2020) Naoya Hino et al.   DEVELOPMENTAL CELL

Phase Separation of a PKA Regulatory Subunit Controls cAMP Compartmentation and Oncogenic Signaling

(2020) Jason Z. Zhang et al.   CELL

Optical Mapping of cAMP Signaling at the Nanometer Scale

(2020) Andreas Bock et al.   CELL

Live Visualization of ERK Activity in the Mouse Blastocyst Reveals Lineage-Specific Signaling Dynamics

(2020) Claire S. Simon et al.   DEVELOPMENTAL CELL

Cell-Cycle-Dependent ERK Signaling Dynamics Direct Fate Specification in the Mammalian Preimplantation Embryo

(2020) Michael J. Pokrass et al.   DEVELOPMENTAL CELL

AKAP Signaling Islands: Venues for Precision Pharmacology

(2020) Mitchell H. Omar et al.   TRENDS IN PHARMACOLOGICAL SCIENCES

Spatial Multiplexing of Fluorescent Reporters for Imaging Signaling Network Dynamics

(2020) Changyang Linghu et al.   CELL

Subcellular Organization of the cAMP Signaling Pathway

(2020) Manuela Zaccolo et al.   PHARMACOLOGICAL REVIEWS

Location-specific inhibition of Akt reveals regulation of mTORC1 activity in the nucleus

(2020) Xin Zhou et al.   Nature Communications

Spatially compartmentalized phase regulation of a Ca2+-cAMP-PKA oscillatory circuit

(2020) Brian Tenner et al.   eLife

Unraveling the multifaceted nature of the nuclear function of mTOR

(2020) Alek S. Torres et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH

Integrative single-cell analysis

(2019) Tim Stuart et al.   NATURE REVIEWS GENETICS

AMP-activated protein kinase: the current landscape for drug development

(2019) Gregory R. Steinberg et al.   NATURE REVIEWS DRUG DISCOVERY

AKT as a Therapeutic Target for Cancer

(2019) Mengqiu Song et al.   CANCER RESEARCH

Visualizing Engrafted Human Cancer and Therapy Responses in Immunodeficient Zebrafish

(2019) Chuan Yan et al.   CELL

Lysosome Positioning Influences mTORC2 and AKT Signaling

(2019) Rui Jia et al.   MOLECULAR CELL

Comprehensive Integration of Single-Cell Data

(2019) Tim Stuart et al.   CELL

Akt-ing Up Just About Everywhere: Compartment-Specific Akt Activation and Function in Receptor Tyrosine Kinase Signaling

(2019) Michael G. Sugiyama et al.   Frontiers in Cell and Developmental Biology

Mathematical Models for Cell Migration with Real-Time Cell Cycle Dynamics

(2018) Sean T. Vittadello et al.   BIOPHYSICAL JOURNAL

Calcium signaling and cell cycle: Progression or death

(2018) Juliette Humeau et al.   CELL CALCIUM

SypHer3s: a genetically encoded fluorescent ratiometric probe with enhanced brightness and an improved dynamic range

(2018) Y. G. Ermakova et al.   CHEMICAL COMMUNICATIONS

Tracking neural crest cell cycle progression in vivo

(2018) Sriivatsan G. Rajan et al.   GENESIS

A Precise Cdk Activity Threshold Determines Passage through the Restriction Point

(2018) Clayton Schwarz et al.   MOLECULAR CELL

Cell cycle heterogeneity directs the timing of neural stem cell activation from quiescence

(2018) L. Otsuki et al.   SCIENCE

Lack of beta-arrestin signaling in the absence of active G proteins

(2018) Manuel Grundmann et al.   Nature Communications

Characterization of Split Fluorescent Protein Variants and Quantitative Analyses of Their Self-Assembly Process

(2018) Tuğba Köker et al.   Scientific Reports

Encoding Growth Factor Identity in the Temporal Dynamics of FOXO3 under the Combinatorial Control of ERK and AKT Kinases

(2018) Somponnat Sampattavanich et al.   Cell Systems

Efficient proximity labeling in living cells and organisms with TurboID

(2018) Tess C Branon et al.   NATURE BIOTECHNOLOGY

Single-fluorophore biosensors for sensitive and multiplexed detection of signalling activities

(2018) Sohum Mehta et al.   NATURE CELL BIOLOGY

Manifold roles of β-arrestins in GPCR signaling elucidated with siRNA and CRISPR/Cas9

(2018) Louis M. Luttrell et al.   Science Signaling

A high-avidity biosensor reveals plasma membrane PI(3,4)P2 is predominantly a class I PI3K signaling product

(2018) Brady D. Goulden et al.   JOURNAL OF CELL BIOLOGY

Super-resolution microscopy demystified

(2018) Lothar Schermelleh et al.   NATURE CELL BIOLOGY

Functional selectivity of GPCR-directed drug action through location bias

(2017) Roshanak Irannejad et al.   Nature Chemical Biology

AKAP-mediated feedback control of cAMP gradients in developing hippocampal neurons

(2017) Kirill Gorshkov et al.   Nature Chemical Biology

Genetically encoded biosensors for visualizing live-cell biochemical activity at super-resolution

(2017) Gary C H Mo et al.   NATURE METHODS

Live-cell measurements of kinase activity in single cells using translocation reporters

(2017) Takamasa Kudo et al.   Nature Protocols

AMPK: guardian of metabolism and mitochondrial homeostasis

(2017) Sébastien Herzig et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Experimental and mathematical analysis of cAMP nanodomains

(2017) Christian Lohse et al.   PLoS One

Genetic evidence that β-arrestins are dispensable for the initiation of β2-adrenergic receptor signaling to ERK

(2017) Morgan O’Hayre et al.   Science Signaling

A Highly Sensitive FRET Biosensor for AMPK Exhibits Heterogeneous AMPK Responses among Cells and Organs

(2017) Yumi Konagaya et al.   Cell Reports

Akt regulation of glycolysis mediates bioenergetic stability in epithelial cells

(2017) Yin P Hung et al.   eLife

Genetic evidence that β-arrestins are dispensable for the initiation of β2-adrenergic receptor signaling to ERK

(2017) Morgan O’Hayre et al.   Science Signaling

Proliferation, survival and metabolism: the role of PI3K/AKT/mTOR signalling in pluripotency and cell fate determination

(2016) Jason S. L. Yu et al.   DEVELOPMENT

Fluorescent indicators for simultaneous reporting of all four cell cycle phases

(2016) Bryce T Bajar et al.   NATURE METHODS

A far-red fluorescent protein evolved from a cyanobacterial phycobiliprotein

(2016) Erik A Rodriguez et al.   NATURE METHODS

Spatially resolved proteomic mapping in living cells with the engineered peroxidase APEX2

(2016) Victoria Hung et al.   Nature Protocols

A comprehensive map of molecular drug targets

(2016) Rita Santos et al.   NATURE REVIEWS DRUG DISCOVERY

A Guide to Fluorescent Protein FRET Pairs

(2016) Bryce Bajar et al.   SENSORS

Evolving Lessons on the Complex Role of AMPK in Normal Physiology and Cancer

(2016) Biplab Dasgupta et al.   TRENDS IN PHARMACOLOGICAL SCIENCES

Versatile protein tagging in cells with split fluorescent protein

(2016) Daichi Kamiyama et al.   Nature Communications

Bright monomeric near-infrared fluorescent proteins as tags and biosensors for multiscale imaging

(2016) Daria M. Shcherbakova et al.   Nature Communications

Mechanisms Restricting Diffusion of Intracellular cAMP

(2016) Shailesh R. Agarwal et al.   Scientific Reports

Fucci-guided purification of hematopoietic stem cells with high repopulating activity

(2015) Masahiro Yo et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Fluorescent ratiometric pH indicator SypHer2: Applications in neuroscience and regenerative biology

(2015) Mikhail E. Matlashov et al.   BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS

Spatial Control of Biochemical Modification Cascades and Pathways

(2015) Aiman Alam-Nazki et al.   BIOPHYSICAL JOURNAL

Quantitative imaging with Fucci and mathematics to uncover temporal dynamics of cell cycle progression

(2015) Takashi Saitou et al.   DEVELOPMENT GROWTH & DIFFERENTIATION

Receptor Level Mechanisms Are Required for Epidermal Growth Factor (EGF)-stimulated Extracellular Signal-regulated Kinase (ERK) Activity Pulses

(2015) Breanne Sparta et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Lysosomal mTORC2/PHLPP1/Akt Regulate Chaperone-Mediated Autophagy

(2015) Esperanza Arias et al.   MOLECULAR CELL

Frequency modulation of ERK activation dynamics rewires cell fate

(2015) H. Ryu et al.   Molecular Systems Biology

Application guide for omics approaches to cell signaling

(2015) Zhong Yao et al.   Nature Chemical Biology

In vivo cell-cycle profiling in xenograft tumors by quantitative intravital microscopy

(2015) Deepak R Chittajallu et al.   NATURE METHODS

Expansion microscopy

(2015) F. Chen et al.   SCIENCE

ERK reinforces actin polymerization to power persistent edge protrusion during motility

(2015) Michelle C. Mendoza et al.   Science Signaling

Compartmentalized AMPK Signaling Illuminated by Genetically Encoded Molecular Sensors and Actuators

(2015) Takafumi Miyamoto et al.   Cell Reports

Dynamic Visualization of mTORC1 Activity in Living Cells

(2015) Xin Zhou et al.   Cell Reports

Accurate Encoding and Decoding by Single Cells: Amplitude Versus Frequency Modulation

(2015) Gabriele Micali et al.   PLoS Computational Biology

Paracrine communication maximizes cellular response fidelity in wound signaling

(2015) L Naomi Handly et al.   eLife

Intercellular propagation of extracellular signal-regulated kinase activation revealed by in vivo imaging of mouse skin

(2015) Toru Hiratsuka et al.   eLife

ERK reinforces actin polymerization to power persistent edge protrusion during motility

(2015) Michelle C. Mendoza et al.   Science Signaling

Spatial–temporal FUCCI imaging of each cell in a tumor demonstrates locational dependence of cell cycle dynamics and chemoresponsiveness

(2014) Shuya Yano et al.   CELL CYCLE

AMPKα2 translocates into the nucleus and interacts with hnRNP H: Implications in metformin-mediated glucose uptake

(2014) Nami Kim et al.   CELLULAR SIGNALLING

GPCR signaling along the endocytic pathway

(2014) Roshanak Irannejad et al.   CURRENT OPINION IN CELL BIOLOGY

Using Markov State Models to Develop a Mechanistic Understanding of Protein Kinase A Regulatory Subunit RIα Activation in Response to cAMP Binding

(2014) Britton W. Boras et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Where is mTOR and what is it doing there?

(2014) Charles Betz et al.   JOURNAL OF CELL BIOLOGY

Time-lapse imaging of cell cycle dynamics during development in living cardiomyocyte

(2014) Hisayuki Hashimoto et al.   JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY

Real-time cell cycle imaging during melanoma growth, invasion, and drug response

(2014) Nikolas K. Haass et al.   Pigment Cell & Melanoma Research

Stretched cell cycle model for proliferating lymphocytes

(2014) M. R. Dowling et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Detection of a Recurrent DNAJB1-PRKACA Chimeric Transcript in Fibrolamellar Hepatocellular Carcinoma

(2014) J. N. Honeyman et al.   SCIENCE

Targeting PI3K/Akt/mTOR Signaling in Cancer

(2014) Camillo Porta et al.   Frontiers in Oncology

The Cell-Cycle State of Stem Cells Determines Cell Fate Propensity

(2013) Siim Pauklin et al.   CELL

The Proliferation-Quiescence Decision Is Controlled by a Bifurcation in CDK2 Activity at Mitotic Exit

(2013) Sabrina L. Spencer et al.   CELL

AMP Is a True Physiological Regulator of AMP-Activated Protein Kinase by Both Allosteric Activation and Enhancing Net Phosphorylation

(2013) Graeme J. Gowans et al.   Cell Metabolism

Stochastic ERK Activation Induced by Noise and Cell-to-Cell Propagation Regulates Cell Density-Dependent Proliferation

(2013) Kazuhiro Aoki et al.   MOLECULAR CELL

Genetically encoded fluorescent probe to visualize intracellular phosphatidylinositol 3,5-bisphosphate localization and dynamics

(2013) X. Li et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

AKAP signaling complexes: pointing towards the next generation of therapeutic targets?

(2013) Jessica L. Esseltine et al.   TRENDS IN PHARMACOLOGICAL SCIENCES

The emerging multiple roles of nuclear Akt

(2012) Alberto M. Martelli et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH

Live Imaging of Protein Kinase Activities in Transgenic Mice Expressing FRET Biosensors

(2012) Yuji Kamioka et al.   CELL STRUCTURE AND FUNCTION

PKA and PDE4D3 anchoring to AKAP9 provides distinct regulation of cAMP signals at the centrosome

(2012) Anna Terrin et al.   JOURNAL OF CELL BIOLOGY

Frequency-Modulated Pulses of ERK Activity Transmit Quantitative Proliferation Signals

(2012) John G. Albeck et al.   MOLECULAR CELL

Improving FRET dynamic range with bright green and red fluorescent proteins

(2012) Amy J Lam et al.   NATURE METHODS

A short G1 phase is an intrinsic determinant of naïve embryonic stem cell pluripotency

(2012) Diana Coronado et al.   Stem Cell Research

Lengthened G1 Phase Indicates Differentiation Status in Human Embryonic Stem Cells

(2012) Ashley Calder et al.   STEM CELLS AND DEVELOPMENT

AMPK Phosphorylates and Inhibits SREBP Activity to Attenuate Hepatic Steatosis and Atherosclerosis in Diet-Induced Insulin-Resistant Mice

(2011) Yu Li et al.   Cell Metabolism

A Fluorescent Reporter of AMPK Activity and Cellular Energy Stress

(2011) Peiling Tsou et al.   Cell Metabolism

Nuclear translocation of AMPK-α1 potentiates striatal neurodegeneration in Huntington’s disease

(2011) Tz-Chuen Ju et al.   JOURNAL OF CELL BIOLOGY

Protein localization in disease and therapy

(2011) M.-C. Hung et al.   JOURNAL OF CELL SCIENCE

AKAPs: The architectural underpinnings of local cAMP signaling

(2011) Michael D. Kritzer et al.   JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY

Development of an optimized backbone of FRET biosensors for kinases and GTPases

(2011) Naoki Komatsu et al.   MOLECULAR BIOLOGY OF THE CELL

Origin of bistability underlying mammalian cell cycle entry

(2011) G. Yao et al.   Molecular Systems Biology

Structure of a nanobody-stabilized active state of the β2 adrenoceptor

(2011) Søren G. F. Rasmussen et al.   NATURE

AMPK and mTOR regulate autophagy through direct phosphorylation of Ulk1

(2011) Joungmok Kim et al.   NATURE CELL BIOLOGY

Spatial expression of the genome: the signal hypothesis at forty

(2011) Karl S. Matlin   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Visualization of PKA activity in plasma membrane microdomains

(2010) Charlene Depry et al.   Molecular BioSystems

Roles for Growth Factors in Cancer Progression

(2010) Esther Witsch et al.   PHYSIOLOGY

Spatio-temporal correlations can drastically change the response of a MAPK pathway

(2010) K. Takahashi et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The Akt isoforms are present at distinct subcellular locations

(2009) Stacey A. Santi et al.   AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY

Cell Signaling in Space and Time: Where Proteins Come Together and When They're Apart

(2009) J. D. Scott et al.   SCIENCE

Visualizing Spatiotemporal Dynamics of Multicellular Cell-Cycle Progression

(2008) Asako Sakaue-Sawano et al.   CELL

Genetically Encoded Calcium Indicators

(2008) Marco Mank et al.   CHEMICAL REVIEWS

Tracing the Silhouette of Individual Cells in S/G2/M Phases with Fluorescence

(2008) Asako Sakaue-Sawano et al.   CHEMISTRY & BIOLOGY

A bistable Rb–E2F switch underlies the restriction point

(2008) Guang Yao et al.   NATURE CELL BIOLOGY

A genetically encoded fluorescent sensor of ERK activity

(2008) C. D. Harvey et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA