Deciphering cell signaling networks with massively multiplexed biosensor barcoding

Control of osteoblast regeneration by a train of Erk activity waves

(2021) Alessandro De Simone et al.   NATURE

Multiplexed GTPase and GEF biosensor imaging enables network connectivity analysis

(2020) Daniel J. Marston et al.   Nature Chemical Biology

An Excitable Ras/PI3K/ERK Signaling Network Controls Migration and Oncogenic Transformation in Epithelial Cells

(2020) Huiwang Zhan et al.   DEVELOPMENTAL CELL

Spatial Multiplexing of Fluorescent Reporters for Imaging Signaling Network Dynamics

(2020) Changyang Linghu et al.   CELL

FPbase: a community-editable fluorescent protein database

(2019) Talley J. Lambert   NATURE METHODS

Excitable dynamics of Ras triggers spontaneous symmetry breaking of PIP3 signaling in motile cells

(2019) Seiya Fukushima et al.   JOURNAL OF CELL SCIENCE

Protease-activated receptor-2 in endosomes signals persistent pain of irritable bowel syndrome

(2018) Nestor N. Jimenez-Vargas et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Adaptation in Living Systems

(2018) Yuhai Tu et al.   Annual Review of Condensed Matter Physics

Single-fluorophore biosensors for sensitive and multiplexed detection of signalling activities

(2018) Sohum Mehta et al.   NATURE CELL BIOLOGY

Genetically Encoded Fluorescent Biosensors Illuminate the Spatiotemporal Regulation of Signaling Networks

(2018) Eric C. Greenwald et al.   CHEMICAL REVIEWS

Integrating chemical and mechanical signals through dynamic coupling between cellular protrusions and pulsed ERK activation

(2018) Jr-Ming Yang et al.   Nature Communications

Coupled excitable Ras and F-actin activation mediates spontaneous pseudopod formation and directed cell movement

(2017) Peter J. M. van Haastert et al.   MOLECULAR BIOLOGY OF THE CELL

Engineering of mCherry variants with long Stokes shift, red-shifted fluorescence, and low cytotoxicity

(2017) Yi Shen et al.   PLoS One

A Highly Sensitive FRET Biosensor for AMPK Exhibits Heterogeneous AMPK Responses among Cells and Organs

(2017) Yumi Konagaya et al.   Cell Reports

Nestin Expressed by Pre-Existing Cardiomyocytes Recapitulated in Part an Embryonic Phenotype; Suppressive Role of p38 MAPK

(2016) Marc-Andre Meus et al.   JOURNAL OF CELLULAR PHYSIOLOGY

Conversion of graded phosphorylation into switch-like nuclear translocation via autoregulatory mechanisms in ERK signalling

(2016) Yuki Shindo et al.   Nature Communications

A New Generation of FRET Sensors for Robust Measurement of Gαi1, Gαi2 and Gαi3 Activation Kinetics in Single Cells

(2016) Jakobus van Unen et al.   PLoS One

Spatio-temporal co-ordination of RhoA, Rac1 and Cdc42 activation during prototypical edge protrusion and retraction dynamics

(2016) Katrin Martin et al.   Scientific Reports

SrGAP2-Dependent Integration of Membrane Geometry and Slit-Robo-Repulsive Cues Regulates Fibroblast Contact Inhibition of Locomotion

(2015) Rafael Dominik Fritz et al.   DEVELOPMENTAL CELL

Polarized activities of AMPK and BRSK in primary hippocampal neurons

(2015) Vedangi Sample et al.   MOLECULAR BIOLOGY OF THE CELL

Locally excitable Cdc42 signals steer cells during chemotaxis

(2015) Hee Won Yang et al.   NATURE CELL BIOLOGY

Compartmentalized AMPK Signaling Illuminated by Genetically Encoded Molecular Sensors and Actuators

(2015) Takafumi Miyamoto et al.   Cell Reports

Dynamic Visualization of mTORC1 Activity in Living Cells

(2015) Xin Zhou et al.   Cell Reports

Intercellular propagation of extracellular signal-regulated kinase activation revealed by in vivo imaging of mouse skin

(2015) Toru Hiratsuka et al.   eLife

High-Sensitivity Measurements of Multiple Kinase Activities in Live Single Cells

(2014) Sergi Regot et al.   CELL

How to Understand and Outwit Adaptation

(2014) Oliver Hoeller et al.   DEVELOPMENTAL CELL

Fluorescence resonance energy transfer based quantitative analysis of feedforward and feedback loops in epidermal growth factor receptor signaling and the sensitivity to molecular targeting drugs

(2014) Yoshihisa Fujita et al.   FEBS Journal

Comparison of adaptation motifs: temporal, stochastic and spatial responses

(2014) Pablo A. Iglesias et al.   IET Systems Biology

Spatial trigger waves: positive feedback gets you a long way

(2014) Lendert Gelens et al.   MOLECULAR BIOLOGY OF THE CELL

A polarized Ca2+, diacylglycerol and STIM1 signalling system regulates directed cell migration

(2014) Feng-Chiao Tsai et al.   NATURE CELL BIOLOGY

Non-invasive intravital imaging of cellular differentiation with a bright red-excitable fluorescent protein

(2014) Jun Chu et al.   NATURE METHODS

Calmodulin-controlled spatial decoding of oscillatory Ca2+ signals by calcineurin

(2014) Sohum Mehta et al.   eLife

Stochastic ERK Activation Induced by Noise and Cell-to-Cell Propagation Regulates Cell Density-Dependent Proliferation

(2013) Kazuhiro Aoki et al.   MOLECULAR CELL

Ultrasensitive fluorescent proteins for imaging neuronal activity

(2013) Tsai-Wen Chen et al.   NATURE

An excitable signal integrator couples to an idling cytoskeletal oscillator to drive cell migration

(2013) Chuan-Hsiang Huang et al.   NATURE CELL BIOLOGY

Near-infrared fluorescent proteins for multicolor in vivo imaging

(2013) Daria M Shcherbakova et al.   NATURE METHODS

A Versatile Toolkit to Produce Sensitive FRET Biosensors to Visualize Signaling in Time and Space

(2013) R. D. Fritz et al.   Science Signaling

Fiji: an open-source platform for biological-image analysis

(2012) Johannes Schindelin et al.   NATURE METHODS

NIH Image to ImageJ: 25 years of image analysis

(2012) Caroline A Schneider et al.   NATURE METHODS

A Fluorescent Reporter of AMPK Activity and Cellular Energy Stress

(2011) Peiling Tsou et al.   Cell Metabolism

A FRET-Based Biosensor for Imaging SYK Activities in Living Cells

(2011) Xue Xiang et al.   Cellular and Molecular Bioengineering

Genetically Encodable Fluorescent Biosensors for Tracking Signaling Dynamics in Living Cells

(2011) Robert H. Newman et al.   CHEMICAL REVIEWS

Analysis of the cellular uptake and nuclear delivery of insulin-like growth factor binding protein-3 in human osteosarcoma cells

(2011) Lucia Micutkova et al.   INTERNATIONAL JOURNAL OF CANCER

Development of an optimized backbone of FRET biosensors for kinases and GTPases

(2011) Naoki Komatsu et al.   MOLECULAR BIOLOGY OF THE CELL

Imaging the coordination of multiple signalling activities in living cells

(2011) Christopher M. Welch et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Detection of focal adhesion kinase activation at membrane microdomains by fluorescence resonance energy transfer

(2011) Jihye Seong et al.   Nature Communications

Rho-kinase/ROCK: A key regulator of the cytoskeleton and cell polarity

(2010) Mutsuki Amano et al.   Cytoskeleton

The FERM domain: organizing the structure and function of FAK

(2010) Margaret C. Frame et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Visualization of JNK activity dynamics with a genetically encoded fluorescent biosensor

(2010) M. Fosbrink et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cells navigate with a local-excitation, global-inhibition-biased excitable network

(2010) Y. Xiong et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Monomeric red fluorescent proteins with a large Stokes shift

(2010) K. D. Piatkevich et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Defining Network Topologies that Can Achieve Biochemical Adaptation

(2009) Wenzhe Ma et al.   CELL

Coordination of Rho GTPase activities during cell protrusion

(2009) Matthias Machacek et al.   NATURE

Photoconversion in orange and red fluorescent proteins

(2009) Gert-Jan Kremers et al.   NATURE METHODS

Multiplexed FRET to Image Multiple Signaling Events in Live Cells

(2008) David M. Grant et al.   BIOPHYSICAL JOURNAL

Improving the photostability of bright monomeric orange and red fluorescent proteins

(2008) Nathan C Shaner et al.   NATURE METHODS

Lifeact: a versatile marker to visualize F-actin

(2008) Julia Riedl et al.   NATURE METHODS

Determination of hierarchical relationship of Src and Rac at subcellular locations with FRET biosensors

(2008) M. Ouyang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A genetically encoded fluorescent sensor of ERK activity

(2008) C. D. Harvey et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA