A Guide to Fluorescent Protein FRET Pairs

A bright cyan-excitable orange fluorescent protein facilitates dual-emission microscopy and enhances bioluminescence imaging in vivo

(2016) Jun Chu et al.   NATURE BIOTECHNOLOGY

Improving brightness and photostability of green and red fluorescent proteins for live cell imaging and FRET reporting

(2016) Bryce T. Bajar et al.   Scientific Reports

pH sensitivity of FRET reporters based on cyan and yellow fluorescent proteins

(2015) Dahdjim-Benoît Betolngar et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Green Fluorescent Protein with Anionic Tryptophan-Based Chromophore and Long Fluorescence Lifetime

(2015) Karen S. Sarkisyan et al.   BIOPHYSICAL JOURNAL

Genetically encoded sensors of protein hydrodynamics and molecular proximity

(2015) Alexander C. Hoepker et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A high speed multifocal multiphoton fluorescence lifetime imaging microscope for live-cell FRET imaging

(2015) Simon P. Poland et al.   Biomedical Optics Express

A dark green fluorescent protein as an acceptor for measurement of Förster resonance energy transfer

(2015) Hideji Murakoshi et al.   Scientific Reports

Optimizing fluorescent protein trios for 3-Way FRET imaging of protein interactions in living cells

(2015) Brandon L. Scott et al.   Scientific Reports

Fourth-Generation Epac-Based FRET Sensors for cAMP Feature Exceptional Brightness, Photostability and Dynamic Range: Characterization of Dedicated Sensors for FLIM, for Ratiometry and with High Affinity

(2015) Jeffrey Klarenbeek et al.   PLoS One

Fluorescent Protein Based FRET Pairs with Improved Dynamic Range for Fluorescence Lifetime Measurements

(2015) Bobin George Abraham et al.   PLoS One

Quantifying Stickiness: Thermodynamic Characterization of Intramolecular Domain Interactions To Guide the Design of Förster Resonance Energy Transfer Sensors

(2014) Laurens H. Lindenburg et al.   BIOCHEMISTRY

High-Sensitivity Measurements of Multiple Kinase Activities in Live Single Cells

(2014) Sergi Regot et al.   CELL

Phototransformable fluorescent proteins: Future challenges

(2014) Virgile Adam et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Chromophore Photoreduction in Red Fluorescent Proteins Is Responsible for Bleaching and Phototoxicity

(2014) Russell B. Vegh et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Non-invasive intravital imaging of cellular differentiation with a bright red-excitable fluorescent protein

(2014) Jun Chu et al.   NATURE METHODS

High-fidelity optical reporting of neuronal electrical activity with an ultrafast fluorescent voltage sensor

(2014) François St-Pierre et al.   NATURE NEUROSCIENCE

The Use of a Stably Expressed FRET Biosensor for Determining the Potency of Cancer Drugs

(2014) William P. Bozza et al.   PLoS One

Engineering Genetically Encoded FRET Sensors

(2014) Laurens Lindenburg et al.   SENSORS

Monitoring of dual bio-molecular events using FRET biosensors based on mTagBFP/sfGFP and mVenus/mKOκ fluorescent protein pairs

(2013) Ting Su et al.   BIOSENSORS & BIOELECTRONICS

The Proliferation-Quiescence Decision Is Controlled by a Bifurcation in CDK2 Activity at Mitotic Exit

(2013) Sabrina L. Spencer et al.   CELL

mPlum-IFP 1.4 fluorescent fusion protein may display Förster resonance energy transfer associated properties that can be used for near-infrared based reporter gene imaging

(2013) Liang-Ting Lin et al.   JOURNAL OF BIOMEDICAL OPTICS

Ultrasensitive fluorescent proteins for imaging neuronal activity

(2013) Tsai-Wen Chen et al.   NATURE

Engineering of weak helper interactions for high-efficiency FRET probes

(2013) Raik Grünberg et al.   NATURE METHODS

A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum

(2013) Nathan C Shaner et al.   NATURE METHODS

Fluorescence resonance energy transfer microscopy as demonstrated by measuring the activation of the serine/threonine kinase Akt

(2013) Joshua A Broussard et al.   Nature Protocols

N-Way FRET Microscopy of Multiple Protein-Protein Interactions in Live Cells

(2013) Adam D. Hoppe et al.   PLoS One

Correction: Phanta: A Non-Fluorescent Photochromic Acceptor for pcFRET

(2013) Craig Don Paul et al.   PLoS One

Application of FRET probes in the analysis of neuronal plasticity

(2013) Yoshibumi Ueda et al.   Frontiers in Neural Circuits

FRET microscopy in the living cell: Different approaches, strengths and weaknesses

(2012) Sergi Padilla-Parra et al.   BIOESSAYS

Fluorescent proteins for FRET microscopy: Monitoring protein interactions in living cells

(2012) Richard N. Day et al.   BIOESSAYS

Correlating Calcium Binding, Förster Resonance Energy Transfer, and Conformational Change in the Biosensor TN-XXL

(2012) Anselm Geiger et al.   BIOPHYSICAL JOURNAL

Quantitative Intensity-Based FRET Approaches—A Comparative Snapshot

(2012) André Zeug et al.   BIOPHYSICAL JOURNAL

Fluorescence lifetime imaging - techniques and applications

(2012) W. BECKER   JOURNAL OF MICROSCOPY

A Fluorescent Sensor for GABA and Synthetic GABAB Receptor Ligands

(2012) Anastasiya Masharina et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

An Orange Fluorescent Protein with a Large Stokes Shift for Single-Excitation Multicolor FCCS and FRET Imaging

(2012) Daria M. Shcherbakova et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Minimum set of mutations needed to optimize cyan fluorescent proteins for live cell imaging

(2012) Marie Erard et al.   Molecular BioSystems

Improving FRET dynamic range with bright green and red fluorescent proteins

(2012) Amy J Lam et al.   NATURE METHODS

Homodimerization of Amyloid Precursor Protein at the Plasma Membrane: A homoFRET Study by Time-Resolved Fluorescence Anisotropy Imaging

(2012) Viviane Devauges et al.   PLoS One

The Impact of Heterogeneity and Dark Acceptor States on FRET: Implications for Using Fluorescent Protein Donors and Acceptors

(2012) Steven S. Vogel et al.   PLoS One

A monomeric red fluorescent protein with low cytotoxicity

(2012) I.I. Shemiakina et al.   Nature Communications

Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%

(2012) Joachim Goedhart et al.   Nature Communications

Development of Probes for Cellular Functions Using Fluorescent Proteins and Fluorescence Resonance Energy Transfer

(2011) Atsushi Miyawaki   Annual Review of Biochemistry

Spatiotemporal Regulation of Small GTPases as Revealed by Probes Based on the Principle of Förster Resonance Energy Transfer (FRET): Implications for Signaling and Pharmacology

(2011) Etsuko Kiyokawa et al.   Annual Review of Pharmacology and Toxicology

Stable expression of FRET biosensors: A new light in cancer research

(2011) Kazuhiro Aoki et al.   CANCER SCIENCE

Development of an optimized backbone of FRET biosensors for kinases and GTPases

(2011) Naoki Komatsu et al.   MOLECULAR BIOLOGY OF THE CELL

Bright and stable near-infrared fluorescent protein for in vivo imaging

(2011) Grigory S Filonov et al.   NATURE BIOTECHNOLOGY

An infrared fluorescent protein for deeper imaging

(2011) Jérôme Lecoq et al.   NATURE BIOTECHNOLOGY

An Improved Cerulean Fluorescent Protein with Enhanced Brightness and Reduced Reversible Photoswitching

(2011) Michele L. Markwardt et al.   PLoS One

Systematic control of protein interaction using a modular ER/K  -helix linker

(2011) S. Sivaramakrishnan et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Design and application of genetically encoded biosensors

(2011) Amy E. Palmer et al.   TRENDS IN BIOTECHNOLOGY

In vivo fluorescence lifetime tomography of a FRET probe expressed in mouse

(2011) James McGinty et al.   Biomedical Optics Express

Three-Color Spectral FRET Microscopy Localizes Three Interacting Proteins in Living Cells

(2010) Yuansheng Sun et al.   BIOPHYSICAL JOURNAL

Far-Red Fluorescent Protein Excitable with Red Lasers for Flow Cytometry and Superresolution STED Nanoscopy

(2010) Kateryna S. Morozova et al.   BIOPHYSICAL JOURNAL

Simultaneous Visualization of Protumorigenic Src and MT1-MMP Activities with Fluorescence Resonance Energy Transfer

(2010) M. Ouyang et al.   CANCER RESEARCH

Red Fluorescent Protein with Reversibly Photoswitchable Absorbance for Photochromic FRET

(2010) Fedor V. Subach et al.   CHEMISTRY & BIOLOGY

Fluorescence Resonance Energy Transfer Biosensors for Cancer Detection and Evaluation of Drug Efficacy

(2010) S. Lu et al.   CLINICAL CANCER RESEARCH

FRET microscopy: from principle to routine technology in cell biology

(2010) A. PIETRASZEWSKA-BOGIEL et al.   JOURNAL OF MICROSCOPY

Spontaneous network activity visualized by ultrasensitive Ca2+ indicators, yellow Cameleon-Nano

(2010) Kazuki Horikawa et al.   NATURE METHODS

Bright cyan fluorescent protein variants identified by fluorescence lifetime screening

(2010) Joachim Goedhart et al.   NATURE METHODS

Near-infrared fluorescent proteins

(2010) Dmitry Shcherbo et al.   NATURE METHODS

Far-red fluorescent tags for protein imaging in living tissues

(2009) Dmitry Shcherbo et al.   BIOCHEMICAL JOURNAL

Homo-FRET Imaging Enables Quantification of Protein Cluster Sizes with Subcellular Resolution

(2009) Arjen N. Bader et al.   BIOPHYSICAL JOURNAL

Fluorescence lifetime and polarization-resolved imaging in cell biology

(2009) James A Levitt et al.   CURRENT OPINION IN BIOTECHNOLOGY

Photophysical properties of Cerulean and Venus fluorescent proteins

(2009) Pabak Sarkar et al.   JOURNAL OF BIOMEDICAL OPTICS

Time-lapse FRET microscopy using fluorescence anisotropy

(2009) D.R. MATTHEWS et al.   JOURNAL OF MICROSCOPY

Activation of CaMKII in single dendritic spines during long-term potentiation

(2009) Seok-Jin R. Lee et al.   NATURE

mRuby, a Bright Monomeric Red Fluorescent Protein for Labeling of Subcellular Structures

(2009) Simone Kredel et al.   PLoS One

Simultaneous Live Cell Imaging Using Dual FRET Sensors with a Single Excitation Light

(2009) Yusuke Niino et al.   PLoS One

Mammalian Expression of Infrared Fluorescent Proteins Engineered from a Bacterial Phytochrome

(2009) X. Shu et al.   SCIENCE

pHlameleons: A Family of FRET-Based Protein Sensors for Quantitative pH Imaging†

(2008) Alessandro Esposito et al.   BIOCHEMISTRY

Multiplexed FRET to Image Multiple Signaling Events in Live Cells

(2008) David M. Grant et al.   BIOPHYSICAL JOURNAL

Conversion of Red Fluorescent Protein into a Bright Blue Probe

(2008) Oksana M. Subach et al.   CHEMISTRY & BIOLOGY

Fluorescent proteins for single-molecule fluorescence applications

(2008) Britta Seefeldt et al.   Journal of Biophotonics

Improving membrane voltage measurements using FRET with new fluorescent proteins

(2008) Hidekazu Tsutsui et al.   NATURE METHODS

Fluorescent protein FRET pairs for ratiometric imaging of dual biosensors

(2008) Hui-wang Ai et al.   NATURE METHODS

Improving the photostability of bright monomeric orange and red fluorescent proteins

(2008) Nathan C Shaner et al.   NATURE METHODS