A Highly Sensitive Fluorescent Akt Biosensor Reveals Lysosome-Selective Regulation of Lipid Second Messengers and Kinase Activity

Akt Is S-Palmitoylated: A New Layer of Regulation for Akt

(2021) Matías Blaustein et al.   Frontiers in Cell and Developmental Biology

Biochemical Activity Architectures Visualized–Using Genetically Encoded Fluorescent Biosensors to Map the Spatial Boundaries of Signaling Compartments

(2021) Sohum Mehta et al.   ACCOUNTS OF CHEMICAL RESEARCH

INPP4B promotes PI3Kα-dependent late endosome formation and Wnt/β-catenin signaling in breast cancer

(2021) Samuel J. Rodgers et al.   Nature Communications

Illuminating the kinome: Visualizing real-time kinase activity in biological systems using genetically encoded fluorescent protein-based biosensors

(2020) Danielle L. Schmitt et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Signaling diversity enabled by Rap1-regulated plasma membrane ERK with distinct temporal dynamics

(2020) Jeremiah Keyes et al.   eLife

Molecular mechanism of Fast Endophilin-Mediated Endocytosis

(2020) Alessandra Casamento et al.   BIOCHEMICAL JOURNAL

Genetically Encodable Fluorescent and Bioluminescent Biosensors Light Up Signaling Networks

(2020) Xin Zhou et al.   TRENDS IN BIOCHEMICAL SCIENCES

The INPP4B Tumor Suppressor Modulates EGFR Trafficking and Promotes Triple-Negative Breast Cancer

(2020) Hui Liu et al.   Cancer Discovery

An ultrasensitive biosensor for high-resolution kinase activity imaging in awake mice

(2020) Jin-Fan Zhang et al.   Nature Chemical Biology

Location-specific inhibition of Akt reveals regulation of mTORC1 activity in the nucleus

(2020) Xin Zhou et al.   Nature Communications

Understanding phosphoinositides: rare, dynamic, and essential membrane phospholipids

(2019) Eamonn J. Dickson et al.   BIOCHEMICAL JOURNAL

Biophysical basis underlying dynamic Lck activation visualized by ZapLck FRET biosensor

(2019) Rongxue Wan et al.   Science Advances

Acylation – A New Means to Control Traffic Through the Golgi

(2019) Andreas M. Ernst et al.   Frontiers in Cell and Developmental Biology

Akt-ing Up Just About Everywhere: Compartment-Specific Akt Activation and Function in Receptor Tyrosine Kinase Signaling

(2019) Michael G. Sugiyama et al.   Frontiers in Cell and Developmental Biology

Molecular organization of mammalian meiotic chromosome axis revealed by expansion STORM microscopy

(2019) Huizhong Xu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Expansion Microscopy: Scalable and Convenient Super-Resolution Microscopy

(2019) Paul W. Tillberg et al.   Annual Review of Cell and Developmental Biology

Quantification of ERK Kinase Activity in Biological Samples Using Differential Sensing

(2019) Diana Zamora-Olivares et al.   ACS Chemical Biology

AKTivation mechanisms

(2019) Philip A Cole et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Regulation of Clathrin-Mediated Endocytosis

(2018) Marcel Mettlen et al.   Annual Review of Biochemistry

Live-cell Imaging with Genetically Encoded Protein Kinase Activity Reporters

(2018) Gembu Maryu et al.   CELL STRUCTURE AND FUNCTION

Image co-localization – co-occurrence versus correlation

(2018) Jesse S. Aaron et al.   JOURNAL OF CELL SCIENCE

Single-color, ratiometric biosensors for detecting signaling activities in live cells

(2018) Brian L Ross et al.   eLife

Quantitative Lipid Imaging Reveals a New Signaling Function of Phosphatidylinositol-3,4-Bisphophate: Isoform- and Site-Specific Activation of Akt

(2018) Shu-Lin Liu et al.   MOLECULAR CELL

Single-fluorophore biosensors for sensitive and multiplexed detection of signalling activities

(2018) Sohum Mehta et al.   NATURE CELL BIOLOGY

Genetically Encoded Fluorescent Biosensors Illuminate the Spatiotemporal Regulation of Signaling Networks

(2018) Eric C. Greenwald et al.   CHEMICAL REVIEWS

A high-avidity biosensor reveals plasma membrane PI(3,4)P2 is predominantly a class I PI3K signaling product

(2018) Brady D. Goulden et al.   JOURNAL OF CELL BIOLOGY

A Chemical Approach for Profiling Intracellular AKT Signaling Dynamics from Single Cells

(2018) Shiqun Shao et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

The lysosome as a cellular centre for signalling, metabolism and quality control

(2018) Rosalie E. Lawrence et al.   NATURE CELL BIOLOGY

Expansion microscopy: principles and uses in biological research

(2018) Asmamaw T. Wassie et al.   NATURE METHODS

Phosphoinositide conversion in endocytosis and the endolysosomal system

(2017) Alexander Wallroth et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

INPP4B and PTEN Loss Leads to PI-3,4-P2 Accumulation and Inhibition of PI3K in TNBC

(2017) Darien E. Reed et al.   MOLECULAR CANCER RESEARCH

PI(3,4,5)P 3 Engagement Restricts Akt Activity to Cellular Membranes

(2017) Michael Ebner et al.   MOLECULAR CELL

mTORC1 activity repression by late endosomal phosphatidylinositol 3,4-bisphosphate

(2017) Andrea L. Marat et al.   SCIENCE

Recent developments of genetically encoded optical sensors for cell biology

(2016) Andrey Bolbat et al.   BIOLOGY OF THE CELL

Multiplexed Fluorescence Imaging of ERK and Akt Activities and Cell-cycle Progression

(2016) Gembu Maryu et al.   CELL STRUCTURE AND FUNCTION

Mapping growth-factor-modulated Akt signaling dynamics

(2016) Sean M. Gross et al.   JOURNAL OF CELL SCIENCE

Expansion microscopy with conventional antibodies and fluorescent proteins

(2016) Tyler J Chozinski et al.   NATURE METHODS

Akt signaling dynamics in individual cells

(2015) Sean M. Gross et al.   JOURNAL OF CELL SCIENCE

Endomembrane PtdIns(3,4,5)P3 activates the PI3K-Akt pathway

(2015) N. Jethwa et al.   JOURNAL OF CELL SCIENCE

Lysosomal mTORC2/PHLPP1/Akt Regulate Chaperone-Mediated Autophagy

(2015) Esperanza Arias et al.   MOLECULAR CELL

Frequency modulation of ERK activation dynamics rewires cell fate

(2015) H. Ryu et al.   Molecular Systems Biology

Glycogen synthase kinase-3 (GSK3): Regulation, actions, and diseases

(2015) Eleonore Beurel et al.   PHARMACOLOGY & THERAPEUTICS

Expansion microscopy

(2015) F. Chen et al.   SCIENCE

Compartmentalized AMPK Signaling Illuminated by Genetically Encoded Molecular Sensors and Actuators

(2015) Takafumi Miyamoto et al.   Cell Reports

Dynamic Visualization of mTORC1 Activity in Living Cells

(2015) Xin Zhou et al.   Cell Reports

Long-Wavelength Fluorescent Reporters for Monitoring Protein Kinase Activity

(2014) Nathan P. Oien et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Spatial Control of the TSC Complex Integrates Insulin and Nutrient Regulation of mTORC1 at the Lysosome

(2014) Suchithra Menon et al.   CELL

Endophilin marks and controls a clathrin-independent endocytic pathway

(2014) Emmanuel Boucrot et al.   NATURE

Synthesis of Dynole 34-2, Dynole 2-24 and Dyngo 4a for investigating dynamin GTPase

(2014) Mark J Robertson et al.   Nature Protocols

Development of a FRET Biosensor with High Specificity for Akt

(2013) Haruko Miura et al.   CELL STRUCTURE AND FUNCTION

Building a Better Dynasore: The Dyngo Compounds Potently Inhibit Dynamin and Endocytosis

(2013) Adam McCluskey et al.   TRAFFIC

The emerging multiple roles of nuclear Akt

(2012) Alberto M. Martelli et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH

Interrogating Signaling Nodes Involved in Cellular Transformations Using Kinase Activity Probes

(2012) Cliff I. Stains et al.   CHEMISTRY & BIOLOGY

Phosphoinositide 3-kinase signaling pathway mediated by p110α regulates invadopodia formation

(2011) Hideki Yamaguchi et al.   JOURNAL OF CELL BIOLOGY

Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators

(2009) Lin Tian et al.   NATURE METHODS

Phosphorylation keeps PTEN phosphatase closed for business

(2009) A. H. Ross et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Spatiotemporal Analysis of Differential Akt Regulation in Plasma Membrane Microdomains

(2008) Xinxin Gao et al.   MOLECULAR BIOLOGY OF THE CELL

A phosphorylation-dependent intramolecular interaction regulates the membrane association and activity of the tumor suppressor PTEN

(2008) M. Rahdar et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Optical measurement of synaptic glutamate spillover and reuptake by linker optimized glutamate-sensitive fluorescent reporters

(2008) S. A. Hires et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA