Transient Expression of Glycosylated SARS-CoV-2 Antigens in Nicotiana benthamiana

Loss of Spike N370 glycosylation as an important evolutionary event for the enhanced infectivity of SARS-CoV-2

(2022) Shuyuan Zhang et al.   CELL RESEARCH

Safety, immunogenicity, and protection provided by unadjuvanted and adjuvanted formulations of a recombinant plant-derived virus-like particle vaccine candidate for COVID-19 in nonhuman primates

(2022) Stéphane Pillet et al.   Cellular & Molecular Immunology

Broadly neutralizing antibodies overcome SARS-CoV-2 Omicron antigenic shift

(2022) Elisabetta Cameroni et al.   NATURE

Glycosite-deleted mRNA of SARS-CoV-2 spike protein as a broad-spectrum vaccine

(2022) Chung-Yi Wu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

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(2022) Katherine G. Nabel et al.   SCIENCE

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(2022) Ki-Beom Moon et al.   Scientific Reports

Investigating Constraints Along the Plant Secretory Pathway to Improve Production of a SARS-CoV-2 Spike Vaccine Candidate

(2022) Emmanuel Margolin et al.   Frontiers in Plant Science

In Planta Production of the Receptor-Binding Domain From SARS-CoV-2 With Human Blood Group A Glycan Structures

(2022) Julia König-Beihammer et al.   Frontiers in Chemistry

Glycosylation as a key parameter in the design of nucleic acid vaccines

(2022) Ahmet Ozdilek et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Plant‐based expression and characterization of SARS‐CoV‐2 virus‐like particles presenting a native spike protein

(2022) Jae‐Wan Jung et al.   PLANT BIOTECHNOLOGY JOURNAL

Plant molecular farming for the production of valuable proteins – Critical evaluation of achievements and future challenges

(2021) Stefan Schillberg et al.   JOURNAL OF PLANT PHYSIOLOGY

ABO blood group and COVID‐19: a review on behalf of the ISBT COVID‐19 working group

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Lewis A Glycans Are Present on Proteins Involved in Cell Wall Biosynthesis and Appear Evolutionarily Conserved Among Natural Arabidopsis thaliana Accessions

(2021) Gernot Beihammer et al.   Frontiers in Plant Science

Cracking the “Sugar Code”: A Snapshot of N- and O-Glycosylation Pathways and Functions in Plants Cells

(2021) Richard Strasser et al.   Frontiers in Plant Science

Prolyl Hydroxylase Paralogs in Nicotiana benthamiana Show High Similarity With Regard to Substrate Specificity

(2021) Réka Mócsai et al.   Frontiers in Plant Science

Phase 1 randomized trial of a plant-derived virus-like particle vaccine for COVID-19

(2021) Brian J. Ward et al.   NATURE MEDICINE

SARS-CoV-2 Antigens Expressed in Plants Detect Antibody Responses in COVID-19 Patients

(2021) Mohau S. Makatsa et al.   Frontiers in Plant Science

Plant-Produced Receptor-Binding Domain of SARS-CoV-2 Elicits Potent Neutralizing Responses in Mice and Non-human Primates

(2021) Konlavat Siriwattananon et al.   Frontiers in Plant Science

A comprehensive antigen production and characterisation study for easy-to-implement, specific and quantitative SARS-CoV-2 serotests

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Site-Specific Steric Control of SARS-CoV-2 Spike Glycosylation

(2021) Joel D. Allen et al.   BIOCHEMISTRY

A selective sweep in the Spike gene has driven SARS-CoV-2 human adaptation

(2021) Lin Kang et al.   CELL

Identification of lectin receptors for conserved SARS‐CoV‐2 glycosylation sites

(2021) David Hoffmann et al.   EMBO JOURNAL

Lectins enhance SARS-CoV-2 infection and influence neutralizing antibodies

(2021) Florian A. Lempp et al.   NATURE

Plant-produced SARS-CoV-2 receptor binding domain (RBD) variants showed differential binding efficiency with anti-spike specific monoclonal antibodies

(2021) Kaewta Rattanapisit et al.   PLoS One

Plant-Produced Glycosylated and In Vivo Deglycosylated Receptor Binding Domain Proteins of SARS-CoV-2 Induce Potent Neutralizing Responses in Mice

(2021) Tarlan Mamedov et al.   Viruses-Basel

N-Glycosylation of the SARS-CoV-2 Receptor Binding Domain Is Important for Functional Expression in Plants

(2021) Yun-Ji Shin et al.   Frontiers in Plant Science

Site-Specific Glycosylation of Recombinant Viral Glycoproteins Produced in Nicotiana benthamiana

(2021) Emmanuel Margolin et al.   Frontiers in Plant Science

The C-Terminal Half of SARS-CoV-2 Nucleocapsid Protein, Industrially Produced in Plants, Is Valid as Antigen in COVID-19 Serological Tests

(2021) Laura Williams et al.   Frontiers in Plant Science

Plant-Expressed Receptor Binding Domain of the SARS-CoV-2 Spike Protein Elicits Humoral Immunity in Mice

(2021) Puna Maya Maharjan et al.   Vaccines

Potential for Developing Plant-Derived Candidate Vaccines and Biologics against Emerging Coronavirus Infections

(2021) Balamurugan Shanmugaraj et al.   Pathogens

Impact of Expressing Cells on Glycosylation and Glycan of the SARS-CoV-2 Spike Glycoprotein

(2021) Yan Wang et al.   ACS Omega

Bioengineering Strategies for Developing Vaccines against Respiratory Viral Diseases

(2021) Shalini Iyer et al.   CLINICAL MICROBIOLOGY REVIEWS

Impact of Specific N-Glycan Modifications on the Use of Plant-Produced SARS-CoV-2 Antigens in Serological Assays

(2021) Jennifer Schwestka et al.   Frontiers in Plant Science

Production and Characterization of Nucleocapsid and RBD Cocktail Antigens of SARS-CoV-2 in Nicotiana benthamiana Plant as a Vaccine Candidate against COVID-19

(2021) Tarlan Mamedov et al.   Vaccines

Development of a SARS-CoV-2 Vaccine Candidate Using Plant-Based Manufacturing and a Tobacco Mosaic Virus-like Nano-Particle

(2021) Joshua M. Royal et al.   Vaccines

A pneumonia outbreak associated with a new coronavirus of probable bat origin

(2020) Peng Zhou et al.   NATURE

Co‐expression of human calreticulin significantly improves the production of HIV gp140 and other viral glycoproteins in plants

(2020) E Margolin et al.   PLANT BIOTECHNOLOGY JOURNAL

Structure, Function, and Antigenicity of the SARS-CoV-2 Spike Glycoprotein

(2020) Alexandra C. Walls et al.   CELL

SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor

(2020) Markus Hoffmann et al.   CELL

A serological assay to detect SARS-CoV-2 seroconversion in humans

(2020) Fatima Amanat et al.   NATURE MEDICINE

Site-specific glycan analysis of the SARS-CoV-2 spike

(2020) Yasunori Watanabe et al.   SCIENCE

Engineering the Plant Secretory Pathway for the Production of Next-Generation Pharmaceuticals

(2020) Emmanuel A. Margolin et al.   TRENDS IN BIOTECHNOLOGY

Potential Applications of Plant Biotechnology against SARS-CoV-2

(2020) Teresa Capell et al.   TRENDS IN PLANT SCIENCE

Vulnerabilities in coronavirus glycan shields despite extensive glycosylation

(2020) Yasunori Watanabe et al.   Nature Communications

The Impact of Mutations in SARS-CoV-2 Spike on Viral Infectivity and Antigenicity

(2020) Qianqian Li et al.   CELL

A vaccine targeting the RBD of the S protein of SARS-CoV-2 induces protective immunity

(2020) Jingyun Yang et al.   NATURE

Mapping Neutralizing and Immunodominant Sites on the SARS-CoV-2 Spike Receptor-Binding Domain by Structure-Guided High-Resolution Serology

(2020) Luca Piccoli et al.   CELL

Efficacy, immunogenicity, and safety of a plant-derived, quadrivalent, virus-like particle influenza vaccine in adults (18–64 years) and older adults (≥65 years): two multicentre, randomised phase 3 trials

(2020) Brian J Ward et al.   LANCET

Prospects for SARS-CoV-2 diagnostics, therapeutics and vaccines in Africa

(2020) Emmanuel Margolin et al.   NATURE REVIEWS MICROBIOLOGY

Characteristics of SARS-CoV-2 and COVID-19

(2020) Ben Hu et al.   NATURE REVIEWS MICROBIOLOGY

Rapid production of SARS-CoV-2 receptor binding domain (RBD) and spike specific monoclonal antibody CR3022 in Nicotiana benthamiana

(2020) Kaewta Rattanapisit et al.   Scientific Reports

Pilot Production of SARS-CoV-2 Related Proteins in Plants: A Proof of Concept for Rapid Repurposing of Indoor Farms Into Biomanufacturing Facilities

(2020) Borja Diego-Martin et al.   Frontiers in Plant Science

Exploitation of glycosylation in enveloped virus pathobiology

(2019) Yasunori Watanabe et al.   BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS

Genome and transcriptome characterization of the glycoengineered Nicotiana benthamiana line ΔXT/FT

(2019) Matteo Schiavinato et al.   BMC GENOMICS

A homology-guided, genome-based proteome for improved proteomics in the alloploid Nicotiana benthamiana

(2019) Jiorgos Kourelis et al.   BMC GENOMICS

A signal motif retains Arabidopsis ER-α-mannosidase I in the cis-Golgi and prevents enhanced glycoprotein ERAD

(2019) Jennifer Schoberer et al.   Nature Communications

Molecular Pharming for low and middle income countries

(2019) Sheeba Murad et al.   CURRENT OPINION IN BIOTECHNOLOGY

CRISPR/Cas9-mediated knockout of six glycosyltransferase genes in Nicotiana benthamiana for the production of recombinant proteins lacking β-1,2-xylose and core α-1,3-fucose

(2018) Julia Jansing et al.   PLANT BIOTECHNOLOGY JOURNAL

An oligosaccharyltransferase from Leishmania major increases the N-glycan occupancy on recombinant glycoproteins produced in Nicotiana benthamiana

(2018) Alexandra Castilho et al.   PLANT BIOTECHNOLOGY JOURNAL

Plant glyco-biotechnology

(2018) Jennifer Schoberer et al.   SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY

Improvement of the transient expression system for production of recombinant proteins in plants

(2018) Tsuyoshi Yamamoto et al.   Scientific Reports

The Rise and Rise of Nicotiana benthamiana: A Plant for All Reasons

(2018) Julia Bally et al.   Annual Review of Phytopathology

Closing and opening holes in the glycan shield of HIV-1 envelope glycoprotein SOSIP trimers can redirect the neutralizing antibody response to the newly unmasked epitopes

(2018) Rajesh P. Ringe et al.   JOURNAL OF VIROLOGY

Exploring Site-Specific N-Glycosylation of HEK293 and Plant-Produced Human IgA Isotypes

(2017) Kathrin Göritzer et al.   JOURNAL OF PROTEOME RESEARCH

Glycan shield and fusion activation of a deltacoronavirus spike glycoprotein fine-tuned for enteric infections

(2017) Xiaoli Xiong et al.   JOURNAL OF VIROLOGY

Immunogenicity and structures of a rationally designed prefusion MERS-CoV spike antigen

(2017) Jesper Pallesen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Plant-made vaccines and reagents for the One Health initiative

(2017) Edward Peter Rybicki   Human Vaccines & Immunotherapeutics

Trimeric HIV-1-Env Structures Define Glycan Shields from Clades A, B, and G

(2016) Guillaume B.E. Stewart-Jones et al.   CELL

Plant protein glycosylation

(2016) Richard Strasser   GLYCOBIOLOGY

Glycan shield and epitope masking of a coronavirus spike protein observed by cryo-electron microscopy

(2016) Alexandra C Walls et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

A Randomized, Controlled Trial of ZMapp for Ebola Virus Infection

(2016)   NEW ENGLAND JOURNAL OF MEDICINE

The production of human glucocerebrosidase in glyco-engineeredNicotiana benthamianaplants

(2016) Juthamard Limkul et al.   PLANT BIOTECHNOLOGY JOURNAL

Engineering of complex protein sialylation in plants

(2016) Somanath Kallolimath et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Plant-produced biopharmaceuticals: A case of technical developments driving clinical deployment

(2016) G. P. Lomonossoff et al.   SCIENCE

Glycan shield and epitope masking of a coronavirus spike protein observed by cryo-electron microscopy

(2016) Alexandra C Walls et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Transient Glyco-Engineering to Produce Recombinant IgA1 with Defined N- and O-Glycans in Plants

(2016) Martina Dicker et al.   Frontiers in Plant Science

Overcoming low yields of plant-made antibodies by a protein engineering approach

(2015) Julia Zischewski et al.   Biotechnology Journal

Bat-to-human: spike features determining ‘host jump’ of coronaviruses SARS-CoV, MERS-CoV, and beyond

(2015) Guangwen Lu et al.   TRENDS IN MICROBIOLOGY

Glycan clustering stabilizes the mannose patch of HIV-1 and preserves vulnerability to broadly neutralizing antibodies

(2015) Laura K. Pritchard et al.   Nature Communications

Overcoming low yields of plant-made antibodies by a protein engineering approach

(2015) Julia Zischewski et al.   Biotechnology Journal

Plant Molecular Pharming for the Treatment of Chronic and Infectious Diseases

(2014) Eva Stoger et al.   Annual Review of Plant Biology

Reversion of advanced Ebola virus disease in nonhuman primates with ZMapp

(2014) Xiangguo Qiu et al.   NATURE

Engineering of Sialylated Mucin-typeO-Glycosylation in Plants

(2012) Alexandra Castilho et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Engineering Mammalian Mucin-typeO-Glycosylation in Plants

(2012) Zhang Yang et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Control of mucin-type O-glycosylation: A classification of the polypeptide GalNAc-transferase gene family

(2011) E. P. Bennett et al.   GLYCOBIOLOGY

Rapid, high-yield production in plants of individualized idiotype vaccines for non-Hodgkin's lymphoma

(2010) M. Bendandi et al.   ANNALS OF ONCOLOGY

The production of hemagglutinin-based virus-like particles in plants: a rapid, efficient and safe response to pandemic influenza

(2010) Marc-André D’Aoust et al.   PLANT BIOTECHNOLOGY JOURNAL

Binding of Herpes Simplex Virus Glycoprotein B (gB) to Paired Immunoglobulin-Like Type 2 Receptor   Depends on Specific Sialylated O-Linked Glycans on gB

(2009) J. Wang et al.   JOURNAL OF VIROLOGY

pEAQ: versatile expression vectors for easy and quick transient expression of heterologous proteins in plants

(2009) Frank Sainsbury et al.   PLANT BIOTECHNOLOGY JOURNAL

Class I  -Mannosidases Are Required for N-Glycan Processing and Root Development in Arabidopsis thaliana

(2009) E. Liebminger et al.   PLANT CELL

Inhibition of the interaction between the SARS-CoV Spike protein and its cellular receptor by anti-histo-blood group antibodies

(2008) Patrice Guillon et al.   GLYCOBIOLOGY

Generation of glyco-engineered Nicotiana benthamiana for the production of monoclonal antibodies with a homogeneous human-like N-glycan structure

(2008) Richard Strasser et al.   PLANT BIOTECHNOLOGY JOURNAL

An efficient plant viral expression system generating orally immunogenic Norwalk virus-like particles

(2008) Luca Santi et al.   VACCINE