Investigating Constraints Along the Plant Secretory Pathway to Improve Production of a SARS-CoV-2 Spike Vaccine Candidate

Challenges in ensuring global access to COVID-19 vaccines: production, affordability, allocation, and deployment

(2021) Olivier J Wouters et al.   LANCET

SARS-CoV-2 variants and ending the COVID-19 pandemic

(2021) Arnaud Fontanet et al.   LANCET

Emergence of a SARS-CoV-2 variant of concern with mutations in spike glycoprotein

(2021) Houriiyah Tegally et al.   NATURE

Sixteen novel lineages of SARS-CoV-2 in South Africa

(2021) Houriiyah Tegally et al.   NATURE MEDICINE

Escape of SARS-CoV-2 501Y.V2 from neutralization by convalescent plasma

(2021) Sandile Cele et al.   NATURE

Phase 1 randomized trial of a plant-derived virus-like particle vaccine for COVID-19

(2021) Brian J. Ward et al.   NATURE MEDICINE

Efficacy of the ChAdOx1 nCoV-19 Covid-19 Vaccine against the B.1.351 Variant

(2021) Shabir A. Madhi et al.   NEW ENGLAND JOURNAL OF MEDICINE

Safety and immunogenicity of a plant-derived rotavirus-like particle vaccine in adults, toddlers and infants

(2021) Natsuki Kurokawa et al.   VACCINE

Plant-Produced Glycosylated and In Vivo Deglycosylated Receptor Binding Domain Proteins of SARS-CoV-2 Induce Potent Neutralizing Responses in Mice

(2021) Tarlan Mamedov et al.   Viruses-Basel

N-Glycosylation of the SARS-CoV-2 Receptor Binding Domain Is Important for Functional Expression in Plants

(2021) Yun-Ji Shin et al.   Frontiers in Plant Science

Site-Specific Glycosylation of Recombinant Viral Glycoproteins Produced in Nicotiana benthamiana

(2021) Emmanuel Margolin et al.   Frontiers in Plant Science

Plant-Expressed Receptor Binding Domain of the SARS-CoV-2 Spike Protein Elicits Humoral Immunity in Mice

(2021) Puna Maya Maharjan et al.   Vaccines

Co‐expression of human calreticulin significantly improves the production of HIV gp140 and other viral glycoproteins in plants

(2020) E Margolin et al.   PLANT BIOTECHNOLOGY JOURNAL

Cryo-EM structure of the 2019-nCoV spike in the prefusion conformation

(2020) Daniel Wrapp et al.   SCIENCE

Engineering the Plant Secretory Pathway for the Production of Next-Generation Pharmaceuticals

(2020) Emmanuel A. Margolin et al.   TRENDS IN BIOTECHNOLOGY

SARS-CoV-2 mRNA vaccine design enabled by prototype pathogen preparedness

(2020) Kizzmekia S. Corbett et al.   NATURE

Isolation of potent SARS-CoV-2 neutralizing antibodies and protection from disease in a small animal model

(2020) Thomas F. Rogers et al.   SCIENCE

Structure-based design of prefusion-stabilized SARS-CoV-2 spikes

(2020) Ching-Lin Hsieh et al.   SCIENCE

Efficacy, immunogenicity, and safety of a plant-derived, quadrivalent, virus-like particle influenza vaccine in adults (18–64 years) and older adults (≥65 years): two multicentre, randomised phase 3 trials

(2020) Brian J Ward et al.   LANCET

Prospects for SARS-CoV-2 diagnostics, therapeutics and vaccines in Africa

(2020) Emmanuel Margolin et al.   NATURE REVIEWS MICROBIOLOGY

Rapid production of SARS-CoV-2 receptor binding domain (RBD) and spike specific monoclonal antibody CR3022 in Nicotiana benthamiana

(2020) Kaewta Rattanapisit et al.   Scientific Reports

Structure, Function, and Antigenicity of the SARS-CoV-2 Spike Glycoprotein

(2020) Alexandra C. Walls et al.   CELL

Identification of two subtilisin‐like serine proteases engaged in the degradation of recombinant proteins in Nicotiana benthamiana

(2020) Alejandro A. Puchol Tarazona et al.   FEBS LETTERS

A Roadmap for the Molecular Farming of Viral Glycoprotein Vaccines: Engineering Glycosylation and Glycosylation-Directed Folding

(2020) Emmanuel Margolin et al.   Frontiers in Plant Science

Prime boost immunisations with DNA, MVA and protein-based vaccines elicit robust HIV-1, Tier 2 neutralizing antibodies against the CAP256 superinfecting virus.

(2019) Michiel T van Diepen et al.   JOURNAL OF VIROLOGY

Production and Immunogenicity of Soluble Plant-Produced HIV-1 Subtype C Envelope gp140 Immunogens

(2019) Emmanuel Margolin et al.   Frontiers in Plant Science

Proteases of Nicotiana benthamiana: an emerging battle for molecular farming

(2019) Philippe V Jutras et al.   CURRENT OPINION IN BIOTECHNOLOGY

Molecular Pharming for low and middle income countries

(2019) Sheeba Murad et al.   CURRENT OPINION IN BIOTECHNOLOGY

Three unrelated protease inhibitors enhance accumulation of pharmaceutical recombinant proteins in Nicotiana benthamiana

(2018) Friederike Grosse-Holz et al.   PLANT BIOTECHNOLOGY JOURNAL

Production of complex viral glycoproteins in plants as vaccine immunogens

(2018) Emmanuel Margolin et al.   PLANT BIOTECHNOLOGY JOURNAL

Immunogenicity and structures of a rationally designed prefusion MERS-CoV spike antigen

(2017) Jesper Pallesen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Commercial-scale biotherapeutics manufacturing facility for plant-made pharmaceuticals

(2015) Barry R. Holtz et al.   PLANT BIOTECHNOLOGY JOURNAL

Site-specific proteolytic degradation of IgG monoclonal antibodies expressed in tobacco plants

(2014) Verena K. Hehle et al.   PLANT BIOTECHNOLOGY JOURNAL

Setting up a platform for plant-based influenza virus vaccine production in South Africa

(2012) Elizabeth Mortimer et al.   BMC BIOTECHNOLOGY

pEAQ: versatile expression vectors for easy and quick transient expression of heterologous proteins in plants

(2009) Frank Sainsbury et al.   PLANT BIOTECHNOLOGY JOURNAL