Overcoming low yields of plant-made antibodies by a protein engineering approach

High-value products from plants: the challenges of process optimization

(2015) Rainer Fischer et al.   CURRENT OPINION IN BIOTECHNOLOGY

Means to optimize protein expression in transgenic plants

(2015) Kristian K Ullrich et al.   CURRENT OPINION IN BIOTECHNOLOGY

The increasing value of plant-made proteins

(2015) Markus Sack et al.   CURRENT OPINION IN BIOTECHNOLOGY

Antibody engineering for increased potency, breadth and half-life

(2015) Stuart A. Sievers et al.   Current Opinion in HIV and AIDS

Engineering Antibodies to Enhance Activity and Increase Half-life

(2014) Stuart A. Sievers et al.   AIDS RESEARCH AND HUMAN RETROVIRUSES

Plant Molecular Pharming for the Treatment of Chronic and Infectious Diseases

(2014) Eva Stoger et al.   Annual Review of Plant Biology

The human anti-HIV antibodies 2F5, 2G12, and PG9 differ in their susceptibility to proteolytic degradation: Down-regulation of endogenous serine and cysteine proteinase activities could improve antibody production in plant-based expression platforms

(2014) Melanie Niemer et al.   Biotechnology Journal

Site-specific proteolytic degradation of IgG monoclonal antibodies expressed in tobacco plants

(2014) Verena K. Hehle et al.   PLANT BIOTECHNOLOGY JOURNAL

Optimal charged mutations in the complementarity-determining regions that prevent domain antibody aggregation are dependent on the antibody scaffold

(2014) J. M. Perchiacca et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Optimizing the Yield of Recombinant Pharmaceutical Proteins in Plants

(2013) Richard Twyman et al.   CURRENT PHARMACEUTICAL DESIGN

Stability engineering of the human antibody repertoire

(2013) Romain Rouet et al.   FEBS LETTERS

Protection of Recombinant Mammalian Antibodies from Development-Dependent Proteolysis in Leaves of Nicotiana benthamiana

(2013) Stéphanie Robert et al.   PLoS One

Predictive models for transient protein expression in tobacco (Nicotiana tabacum L.) can optimize process time, yield, and downstream costs

(2012) J.F. Buyel et al.   BIOTECHNOLOGY AND BIOENGINEERING

Identifying bottlenecks in transient and stable production of recombinant monoclonal-antibody sequence variants in chinese hamster ovary cells

(2012) Megan Mason et al.   BIOTECHNOLOGY PROGRESS

Functional characterization of the recombinant HIV-neutralizing monoclonal antibody 2F5 produced in maize seeds

(2012) M. Sabalza et al.   PLANT MOLECULAR BIOLOGY

Aggregation-resistant domain antibodies engineered with charged mutations near the edges of the complementarity-determining regions

(2012) J. M. Perchiacca et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Engineering Aggregation-Resistant Antibodies

(2012) Joseph M. Perchiacca et al.   Annual Review of Chemical and Biomolecular Engineering

GMP issues for recombinant plant-derived pharmaceutical proteins

(2011) Rainer Fischer et al.   BIOTECHNOLOGY ADVANCES

Plant-made pharmaceuticals: Leading products and production platforms

(2011) Matthew Paul et al.   BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY

Codon usage: Nature's roadmap to expression and folding of proteins

(2011) Evelina Angov   Biotechnology Journal

Bias in the Cq value observed with hydrolysis probe based quantitative PCR can be corrected with the estimated PCR efficiency value

(2010) Jari Michael Tuomi et al.   METHODS

Stable internal reference genes for normalization of real-time RT-PCR in tobacco (Nicotiana tabacum) during development and abiotic stress

(2010) Gregor W. Schmidt et al.   MOLECULAR GENETICS AND GENOMICS

Production of antibodies in plants: status after twenty years

(2010) Benoit De Muynck et al.   PLANT BIOTECHNOLOGY JOURNAL

Monoclonal antibody produced in plants efficiently treats West Nile virus infection in mice

(2010) H. Lai et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Plants as bioreactors: Recent developments and emerging opportunities

(2009) Arun K. Sharma et al.   BIOTECHNOLOGY ADVANCES

An Unfolded CH1 Domain Controls the Assembly and Secretion of IgG Antibodies

(2009) Matthias J. Feige et al.   MOLECULAR CELL

Amplification efficiency: linking baseline and bias in the analysis of quantitative PCR data

(2009) J. M. Ruijter et al.   NUCLEIC ACIDS RESEARCH

Design of therapeutic proteins with enhanced stability

(2009) N. Chennamsetty et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Preventing unintended proteolysis in plant protein biofactories

(2008) Meriem Benchabane et al.   PLANT BIOTECHNOLOGY JOURNAL