N-Glycosylation of the SARS-CoV-2 Receptor Binding Domain Is Important for Functional Expression in Plants

Generation of enzymatically competent SARS‐CoV‐2 decoy receptor ACE2‐Fc in glycoengineered Nicotiana benthamiana

(2021) Alexandra Castilho et al.   Biotechnology Journal

Nanoluciferase complementation-based bioreporter reveals the importance of N-linked glycosylation of SARS-CoV-2 S for viral entry

(2021) Taha Azad et al.   MOLECULAR THERAPY

SARS-CoV-2 Antigens Expressed in Plants Detect Antibody Responses in COVID-19 Patients

(2021) Mohau S. Makatsa et al.   Frontiers in Plant Science

A comprehensive antigen production and characterisation study for easy-to-implement, specific and quantitative SARS-CoV-2 serotests

(2021) Miriam Klausberger et al.   EBioMedicine

Co‐expression of human calreticulin significantly improves the production of HIV gp140 and other viral glycoproteins in plants

(2020) E Margolin et al.   PLANT BIOTECHNOLOGY JOURNAL

Structure, Function, and Antigenicity of the SARS-CoV-2 Spike Glycoprotein

(2020) Alexandra C. Walls et al.   CELL

Calnexin cycle – structural features of the ER chaperone system

(2020) Guennadi Kozlov et al.   FEBS Journal

Cross-neutralization of SARS-CoV-2 by a human monoclonal SARS-CoV antibody

(2020) Dora Pinto et al.   NATURE

Structure of the SARS-CoV-2 spike receptor-binding domain bound to the ACE2 receptor

(2020) Jun Lan et al.   NATURE

A serological assay to detect SARS-CoV-2 seroconversion in humans

(2020) Fatima Amanat et al.   NATURE MEDICINE

Site-specific glycan analysis of the SARS-CoV-2 spike

(2020) Yasunori Watanabe et al.   SCIENCE

A highly conserved cryptic epitope in the receptor binding domains of SARS-CoV-2 and SARS-CoV

(2020) Meng Yuan et al.   SCIENCE

Engineering the Plant Secretory Pathway for the Production of Next-Generation Pharmaceuticals

(2020) Emmanuel A. Margolin et al.   TRENDS IN BIOTECHNOLOGY

Efficient N-Glycosylation of the Heavy Chain Tailpiece Promotes the Formation of Plant-Produced Dimeric IgA

(2020) Kathrin Göritzer et al.   Frontiers in Chemistry

Deep mutational scanning of SARS-CoV-2 receptor binding domain reveals constraints on folding and ACE2 binding

(2020) Tyler N. Starr et al.   CELL

The Impact of Mutations in SARS-CoV-2 Spike on Viral Infectivity and Antigenicity

(2020) Qianqian Li et al.   CELL

A vaccine targeting the RBD of the S protein of SARS-CoV-2 induces protective immunity

(2020) Jingyun Yang et al.   NATURE

Site-specific N-glycosylation Characterization of Recombinant SARS-CoV-2 Spike Proteins

(2020) Yong Zhang et al.   MOLECULAR & CELLULAR PROTEOMICS

Rapid production of SARS-CoV-2 receptor binding domain (RBD) and spike specific monoclonal antibody CR3022 in Nicotiana benthamiana

(2020) Kaewta Rattanapisit et al.   Scientific Reports

Pilot Production of SARS-CoV-2 Related Proteins in Plants: A Proof of Concept for Rapid Repurposing of Indoor Farms Into Biomanufacturing Facilities

(2020) Borja Diego-Martin et al.   Frontiers in Plant Science

Golgi localization of GnTI requires a polar amino acid residue within its transmembrane domain

(2019) Jennifer Schoberer et al.   PLANT PHYSIOLOGY

Distinct Fc alpha receptor N-glycans modulate the binding affinity to immunoglobulin A (IgA) antibodies

(2019) Kathrin Göritzer et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Critical Analysis of the Commercial Potential of Plants for the Production of Recombinant Proteins

(2019) Stefan Schillberg et al.   Frontiers in Plant Science

Production and Immunogenicity of Soluble Plant-Produced HIV-1 Subtype C Envelope gp140 Immunogens

(2019) Emmanuel Margolin et al.   Frontiers in Plant Science

An oligosaccharyltransferase from Leishmania major increases the N-glycan occupancy on recombinant glycoproteins produced in Nicotiana benthamiana

(2018) Alexandra Castilho et al.   PLANT BIOTECHNOLOGY JOURNAL

Production of complex viral glycoproteins in plants as vaccine immunogens

(2018) Emmanuel Margolin et al.   PLANT BIOTECHNOLOGY JOURNAL

The glycan-dependent ERAD machinery degrades topologically diverse misfolded proteins

(2018) Yun-Ji Shin et al.   PLANT JOURNAL

Closing and opening holes in the glycan shield of HIV-1 envelope glycoprotein SOSIP trimers can redirect the neutralizing antibody response to the newly unmasked epitopes

(2018) Rajesh P. Ringe et al.   JOURNAL OF VIROLOGY

Immunogenicity and structures of a rationally designed prefusion MERS-CoV spike antigen

(2017) Jesper Pallesen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Synthetic gene design-The rationale for codon optimization and implications for molecular pharming in plants

(2016) Gina R. Webster et al.   BIOTECHNOLOGY AND BIOENGINEERING

Glycan shield and epitope masking of a coronavirus spike protein observed by cryo-electron microscopy

(2016) Alexandra C Walls et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Reduced paucimannosidicN-glycan formation by suppression of a specific β-hexosaminidase fromNicotiana benthamiana

(2016) Yun-Ji Shin et al.   PLANT BIOTECHNOLOGY JOURNAL

Glycan shield and epitope masking of a coronavirus spike protein observed by cryo-electron microscopy

(2016) Alexandra C Walls et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Using glyco-engineering to produce therapeutic proteins

(2015) Martina Dicker et al.   EXPERT OPINION ON BIOLOGICAL THERAPY

Protein body formation in leaves ofNicotiana benthamiana: a concentration-dependent mechanism influenced by the presence of fusion tags

(2015) Reza Saberianfar et al.   PLANT BIOTECHNOLOGY JOURNAL

Plant Molecular Pharming for the Treatment of Chronic and Infectious Diseases

(2014) Eva Stoger et al.   Annual Review of Plant Biology

A context-independentN-glycan signal targets the misfolded extracellular domain ofArabidopsisSTRUBBELIG to endoplasmic-reticulum-associated degradation

(2014) Silvia Hüttner et al.   BIOCHEMICAL JOURNAL

Biochemical composition of haemagglutinin-based influenza virus-like particle vaccine produced by transient expression in tobacco plants

(2014) François Le Mauff et al.   PLANT BIOTECHNOLOGY JOURNAL

Arabidopsis Class I  -Mannosidases MNS4 and MNS5 Are Involved in Endoplasmic Reticulum-Associated Degradation of Misfolded Glycoproteins

(2014) S. Huttner et al.   PLANT CELL

Monitoring of S Protein Maturation in the Endoplasmic Reticulum by Calnexin Is Important for the Infectivity of Severe Acute Respiratory Syndrome Coronavirus

(2012) M. Fukushi et al.   JOURNAL OF VIROLOGY

Endoplasmic Reticulum-Associated Degradation of Glycoproteins in Plants

(2012) Silvia Hüttner et al.   Frontiers in Plant Science

N-Glycosylation engineering of plants for the biosynthesis of glycoproteins with bisected and branched complex N-glycans

(2011) Alexandra Castilho et al.   GLYCOBIOLOGY

Expression of an immunogenic Ebola immune complex in Nicotiana benthamiana

(2011) Waranyoo Phoolcharoen et al.   PLANT BIOTECHNOLOGY JOURNAL

A dual regulatory role of Arabidopsis calreticulin-2 in plant innate immunity

(2011) Yongjian Qiu et al.   PLANT JOURNAL

Arabidopsis thalianaalpha1,2-glucosyltransferase (ALG10) is required for efficient N-glycosylation and leaf growth

(2011) Akhlaq Farid et al.   PLANT JOURNAL

Mapping of Domains on HIV Envelope Protein Mediating Association with Calnexin and Protein-disulfide Isomerase

(2010) Marie-Jeanne Papandréou et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Production of monoclonal antibodies with a controlled N-glycosylation pattern in seeds of Arabidopsis thaliana

(2010) Andreas Loos et al.   PLANT BIOTECHNOLOGY JOURNAL

Induction of protein body formation in plant leaves by elastin-like polypeptide fusions

(2009) Andrew J Conley et al.   BMC BIOLOGY

Receptor quality control in the endoplasmic reticulum for plant innate immunity

(2009) Yusuke Saijo et al.   EMBO JOURNAL

pEAQ: versatile expression vectors for easy and quick transient expression of heterologous proteins in plants

(2009) Frank Sainsbury et al.   PLANT BIOTECHNOLOGY JOURNAL

A plant-specific calreticulin is a key retention factor for a defective brassinosteroid receptor in the endoplasmic reticulum

(2009) H. Jin et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Specific ER quality control components required for biogenesis of the plant innate immune receptor EFR

(2009) J. Li et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Plant-produced vaccines: promise and reality

(2008) Edward P. Rybicki   DRUG DISCOVERY TODAY

Functional Characterization of Arabidopsis Calreticulin1a: A Key Alleviator of Endoplasmic Reticulum Stress

(2008) A. Christensen et al.   PLANT AND CELL PHYSIOLOGY

Generation of glyco-engineered Nicotiana benthamiana for the production of monoclonal antibodies with a homogeneous human-like N-glycan structure

(2008) Richard Strasser et al.   PLANT BIOTECHNOLOGY JOURNAL

Multiple Mechanism-Mediated Retention of a Defective Brassinosteroid Receptor in the Endoplasmic Reticulum of Arabidopsis

(2008) Z. Hong et al.   PLANT CELL