Membrane Protein Stabilization Strategies for Structural and Functional Studies

Megabodies expand the nanobody toolkit for protein structure determination by single-particle cryo-EM

(2021) Tomasz Uchański et al.   NATURE METHODS

Structure and Functional Characterization of Membrane Integral Proteins in the Lipid Cubic Phase

(2020) Dianfan Li et al.   JOURNAL OF MOLECULAR BIOLOGY

Spin-labeled nanobodies as protein conformational reporters for electron paramagnetic resonance in cellular membranes

(2020) Laura Galazzo et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Nanodisc technology facilitates identification of monoclonal antibodies targeting multi-pass membrane proteins

(2020) Bernd Gardill et al.   Scientific Reports

Consensus Mutagenesis and Ancestral Reconstruction Provide Insight into the Substrate Specificity and Evolution of the Front-End Δ6-Desaturase Family

(2020) Dongdi Li et al.   BIOCHEMISTRY

Cryo-EM structure of the human concentrative nucleoside transporter CNT3

(2020) Yanxia Zhou et al.   PLOS BIOLOGY

Large Nanodiscs: A Potential Game Changer in Structural Biology of Membrane Protein Complexes and Virus Entry

(2020) Krishna M. Padmanabha Das et al.   Frontiers in Bioengineering and Biotechnology

Structure and Dynamics of GPCRs in Lipid Membranes: Physical Principles and Experimental Approaches

(2020) Andrew J. Y. Jones et al.   MOLECULES

Consensus mutagenesis approach improves the thermal stability of system x c ‐ transporter, xCT , and enables cryo‐EM analyses

(2020) Kazumasa Oda et al.   PROTEIN SCIENCE

ATP modulates SLC7A5 (LAT1) synergistically with cholesterol

(2020) Jessica Cosco et al.   Scientific Reports

GFP Fusion to the N-Terminus of MotB Affects the Proton Channel Activity of the Bacterial Flagellar Motor in Salmonella

(2020) Yusuke V. Morimoto et al.   Biomolecules

An improved fluorescent tag and its nanobodies for membrane protein expression, stability assay, and purification

(2020) Hongmin Cai et al.   Communications Biology

Structure of the human LAT1–4F2hc heteromeric amino acid transporter complex

(2019) Renhong Yan et al.   NATURE

Functional characterization of the alanine-serine-cysteine exchanger of Carnobacterium sp AT7

(2019) Paola Bartoccioni et al.   JOURNAL OF GENERAL PHYSIOLOGY

Distribution of Proteins at the Inner Nuclear Membrane Is Regulated by the Asi1 E3 Ligase in Saccharomyces cerevisiae

(2019) Christine J. Smoyer et al.   GENETICS

Split Green Fluorescent Proteins: Scope, Limitations, and Outlook

(2019) Matthew G. Romei et al.   Annual Review of Biophysics

Selection of Biophysical Methods for Characterisation of Membrane Proteins

(2019) Tristan O. C. Kwan et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Serotonin transporter–ibogaine complexes illuminate mechanisms of inhibition and transport

(2019) Jonathan A. Coleman et al.   NATURE

Consensus sequence design as a general strategy to create hyperstable, biologically active proteins

(2019) Matt Sternke et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cryo-EM structure of the human L-type amino acid transporter 1 in complex with glycoprotein CD98hc

(2019) Yongchan Lee et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

L amino acid transporter structure and molecular bases for the asymmetry of substrate interaction

(2019) Ekaitz Errasti-Murugarren et al.   Nature Communications

High-throughput stability screening for detergent-solubilized membrane proteins

(2019) Vadim Kotov et al.   Scientific Reports

Cryo-EM structures and functional characterization of murine Slc26a9 reveal mechanism of uncoupled chloride transport

(2019) Justin D Walter et al.   eLife

Membrane Structure–Function Insights from Asymmetric Lipid Vesicles

(2019) Erwin London   ACCOUNTS OF CHEMICAL RESEARCH

The lipid-dependent structure and function of LacY can be recapitulated and analyzed in phospholipid-containing detergent micelles

(2019) Heidi Vitrac et al.   Scientific Reports

Interactions between amyloid β peptide and lipid membranes

(2018) Zheng Niu et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Modulation of Membrane Proteins by Lipids

(2018) Carmen Domene   BIOPHYSICAL JOURNAL

Perturbations of Native Membrane Protein Structure in Alkyl Phosphocholine Detergents: A Critical Assessment of NMR and Biophysical Studies

(2018) Christophe Chipot et al.   CHEMICAL REVIEWS

Lipid–Protein Interactions Are Unique Fingerprints for Membrane Proteins

(2018) Valentina Corradi et al.   ACS Central Science

Chromobodies to quantify changes of endogenous protein concentration in living cells

(2018) Bettina-Maria Keller et al.   MOLECULAR & CELLULAR PROTEOMICS

Crystal Structure of a ligand-bound LacY–Nanobody Complex

(2018) Hemant Kumar et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Assessment of GFP Tag Position on Protein Localization and Growth Fitness in Yeast

(2018) Uri Weill et al.   JOURNAL OF MOLECULAR BIOLOGY

Consensus designs and thermal stability determinants of a human glutamate transporter

(2018) Erica Cirri et al.   eLife

Structural insights into the extracellular recognition of the human serotonin 2B receptor by an antibody

(2017) Andrii Ishchenko et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Modulation of LAT1 (SLC7A5) transporter activity and stability by membrane cholesterol

(2017) David Dickens et al.   Scientific Reports

The folding, stability and function of lactose permease differ in their dependence on bilayer lipid composition

(2017) Heather E. Findlay et al.   Scientific Reports

Drugging Membrane Protein Interactions

(2016) Hang Yin et al.   Annual Review of Biomedical Engineering

Current strategies for protein production and purification enabling membrane protein structural biology

(2016) Aditya Pandey et al.   Biochemistry and Cell Biology

Chromatibody, a novel non-invasive molecular tool to explore and manipulate chromatin in living cells

(2016) Denis Jullien et al.   JOURNAL OF CELL SCIENCE

AlaScan: A Graphical User Interface for Alanine Scanning Free-Energy Calculations

(2016) Vijayaraj Ramadoss et al.   Journal of Chemical Information and Modeling

Stabilization of a prokaryotic LAT transporter by random mutagenesis

(2016) Arturo Rodríguez-Banqueri et al.   JOURNAL OF GENERAL PHYSIOLOGY

How Do Short Chain Nonionic Detergents Destabilize G-Protein-Coupled Receptors?

(2016) Sangbae Lee et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Membrane Proteins Are Dramatically Less Conserved than Water-Soluble Proteins across the Tree of Life

(2016) Victor Sojo et al.   MOLECULAR BIOLOGY AND EVOLUTION

A mutagenesis and screening strategy to generate optimally thermostabilized membrane proteins for structural studies

(2016) Francesca Magnani et al.   Nature Protocols

Conformational Chaperones for Structural Studies of Membrane Proteins Using Antibody Phage Display with Nanodiscs

(2016) Pawel K. Dominik et al.   STRUCTURE

How Can Mutations Thermostabilize G-Protein-Coupled Receptors?

(2016) Nagarajan Vaidehi et al.   TRENDS IN PHARMACOLOGICAL SCIENCES

Protein stability: a crystallographer's perspective

(2016) Marc C. Deller et al.   Acta Crystallographica Section F-Structural Biology Communications

Cellular growth defects triggered by an overload of protein localization processes

(2016) Reiko Kintaka et al.   Scientific Reports

Controlled Co-reconstitution of Multiple Membrane Proteins in Lipid Bilayer Nanodiscs Using DNA as a Scaffold

(2015) Thomas Raschle et al.   ACS Chemical Biology

Direct Capture of Functional Proteins from Mammalian Plasma Membranes into Nanodiscs

(2015) Jahnabi Roy et al.   BIOCHEMISTRY

Lipid modulation of early G protein-coupled receptor signalling events

(2015) Patricia M. Dijkman et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

The styrene–maleic acid copolymer: a versatile tool in membrane research

(2015) Jonas M. Dörr et al.   EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS

Defining thermostability of membrane proteins by western blotting

(2015) Y. Ashok et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Scintillation proximity assay (SPA) as a new approach to determine a ligand’s kinetic profile. A case in point for the adenosine A1 receptor

(2015) Lizi Xia et al.   Purinergic Signalling

Tissue-based map of the human proteome

(2015) M. Uhlen et al.   SCIENCE

Development of a Thermofluor assay for stability determination of membrane proteins using the Na+/H+antiporter NhaA and cytochromecoxidase

(2015) Martin Kohlstaedt et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Stabilization of G protein-coupled receptors by point mutations

(2015) Franziska M. Heydenreich et al.   Frontiers in Pharmacology

Membrane proteins bind lipids selectively to modulate their structure and function

(2014) Arthur Laganowsky et al.   NATURE

Crystal structure of the human glucose transporter GLUT1

(2014) Dong Deng et al.   NATURE

Structure of class C GPCR metabotropic glutamate receptor 5 transmembrane domain

(2014) Andrew S. Doré et al.   NATURE

A general protocol for the generation of Nanobodies for structural biology

(2014) Els Pardon et al.   Nature Protocols

Detergent-Induced Stabilization and Improved 3D Map of the Human Heteromeric Amino Acid Transporter 4F2hc-LAT2

(2014) Marcel Meury et al.   PLoS One

Membrane protein stability can be compromised by detergent interactions with the extramembranous soluble domains

(2014) Zhengrong Yang et al.   PROTEIN SCIENCE

Nanobodies: Natural Single-Domain Antibodies

(2013) Serge Muyldermans   Annual Review of Biochemistry

Generation of functional antibodies for mammalian membrane protein crystallography

(2013) Tomoya Hino et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Structure of class B GPCR corticotropin-releasing factor receptor 1

(2013) Kaspar Hollenstein et al.   NATURE

X-ray structure of dopamine transporter elucidates antidepressant mechanism

(2013) Aravind Penmatsa et al.   NATURE

Protein Purification-free Method of Binding Affinity Determination by Microscale Thermophoresis

(2013) Lyuba Khavrutskii et al.   Jove-Journal of Visualized Experiments

Efficiency of detergents at maintaining membrane protein structures in their biologically relevant forms

(2012) David V. Tulumello et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

X-ray structures of LeuT in substrate-free outward-open and apo inward-open states

(2012) Harini Krishnamurthy et al.   NATURE

Measuring substrate binding and affinity of purified membrane transport proteins using the scintillation proximity assay

(2012) Daniel Harder et al.   Nature Protocols

Protein engineering methods applied to membrane protein targets

(2012) M. W. Lluis et al.   PROTEIN ENGINEERING DESIGN & SELECTION

A Fluorescence-Detection Size-Exclusion Chromatography-Based Thermostability Assay for Membrane Protein Precrystallization Screening

(2012) Motoyuki Hattori et al.   STRUCTURE

Assembly of Bacterial Inner Membrane Proteins

(2011) Ross E. Dalbey et al.   Annual Review of Biochemistry

Influence of detergents on the activity of the ABC transporter LmrA

(2011) Nacera Infed et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Simple screening method for improving membrane protein thermostability

(2011) Romina Mancusso et al.   METHODS

Crystal structure of the β2 adrenergic receptor–Gs protein complex

(2011) Søren G. F. Rasmussen et al.   NATURE

Overcoming barriers to membrane protein structure determination

(2011) Roslyn M Bill et al.   NATURE BIOTECHNOLOGY

Heterologous Expression of Membrane Proteins: Choosing the Appropriate Host

(2011) Florent Bernaudat et al.   PLoS One

Mechanism for selectivity-inactivation coupling in KcsA potassium channels

(2011) W. W. L. Cheng et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Molecular basis of substrate-induced permeation by an amino acid antiporter

(2011) L. Kowalczyk et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Benchmarking Membrane Protein Detergent Stability for Improving Throughput of High-Resolution X-ray Structures

(2011) Yo Sonoda et al.   STRUCTURE

A Case Of Convergence: Why Did a Simple Alternative to Canonical Antibodies Arise in Sharks and Camels?

(2011) Martin F. Flajnik et al.   PLOS BIOLOGY

A high-throughput differential filtration assay to screen and select detergents for membrane proteins

(2010) James M. Vergis et al.   ANALYTICAL BIOCHEMISTRY

Functional reconstitution of monomeric CYP3A4 with multiple cytochrome P450 reductase molecules in Nanodiscs

(2010) Yelena V. Grinkova et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

In vivo adsorption of autoantibodies in myasthenia gravis using Nanodisc-incorporated acetylcholine receptor

(2010) Jian Rong Sheng et al.   EXPERIMENTAL NEUROLOGY

Membrane protein assembly into Nanodiscs

(2009) Timothy H. Bayburt et al.   FEBS LETTERS

Static light scattering to characterize membrane proteins in detergent solution

(2008) Dirk Jan Slotboom et al.   METHODS

Insights into outer membrane protein crystallization

(2008) Simon Newstead et al.   MOLECULAR MEMBRANE BIOLOGY

Conformational thermostabilization of the  1-adrenergic receptor in a detergent-resistant form

(2008) M. J. Serrano-Vega et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Stable interactions between the transmembrane domains of the adenosine A2Areceptor

(2008) Damien Thévenin et al.   PROTEIN SCIENCE

Microscale Fluorescent Thermal Stability Assay for Membrane Proteins

(2008) Alexander I. Alexandrov et al.   STRUCTURE