Nanobodies: Natural Single-Domain Antibodies

Tetrameric and Homodimeric Camelid IgGs Originate from the Same IgH Locus

(2014) I. Achour et al.   JOURNAL OF IMMUNOLOGY

A bacterial-two-hybrid selection system for one-step isolation of intracellularly functional Nanobodies

(2012) Mireille Pellis et al.   ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS

Nanobodies Targeting Mouse/Human VCAM1 for the Nuclear Imaging of Atherosclerotic Lesions

(2012) Alexis Broisat et al.   CIRCULATION RESEARCH

Cell-penetrating anti-GFAP VHH and corresponding fluorescent fusion protein VHH-GFP spontaneously cross the blood-brain barrier and specifically recognize astrocytes: application to brain imaging

(2012) Tengfei Li et al.   FASEB JOURNAL

A simple, versatile method for GFP-based super-resolution microscopy via nanobodies

(2012) Jonas Ries et al.   NATURE METHODS

Llama Antibody Fragments Recognizing Various Epitopes of the CD4bs Neutralize a Broad Range of HIV-1 Subtypes A, B and C

(2012) Nika Strokappe et al.   PLoS One

The TITAN trial – Assessing the efficacy and safety of an anti-von Willebrand factor Nanobody in patients with acquired thrombotic thrombocytopenic purpura

(2012) Josefin-Beate Holz   TRANSFUSION AND APHERESIS SCIENCE

Specific Cell Targeting with Nanobody Conjugated Branched Gold Nanoparticles for Photothermal Therapy

(2011) Bieke Van de Broek et al.   ACS Nano

Magnetosome Expression of Functional Camelid Antibody Fragments (Nanobodies) in Magnetospirillum gryphiswaldense

(2011) Anna Pollithy et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Immuno-imaging using nanobodies

(2011) Ilse Vaneycken et al.   CURRENT OPINION IN BIOTECHNOLOGY

Engineering antibodies and proteins for molecular in vivo imaging

(2011) Tina Romer et al.   CURRENT OPINION IN BIOTECHNOLOGY

Strategies for extended serum half-life of protein therapeutics

(2011) Roland E Kontermann   CURRENT OPINION IN BIOTECHNOLOGY

Nanobody stabilization of G protein-coupled receptor conformational states

(2011) Jan Steyaert et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Facile labelling of an anti-epidermal growth factor receptor Nanobody with 68Ga via a novel bifunctional desferal chelate for immuno-PET

(2011) Maria J. W. D. Vosjan et al.   EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING

Preclinical screening of anti-HER2 nanobodies for molecular imaging of breast cancer

(2011) Ilse Vaneycken et al.   FASEB JOURNAL

Lactobacilli producing bispecific llama-derived anti-rotavirus proteins in vivo for rotavirus-induced diarrhea

(2011) Neha Pant et al.   Future Microbiology

Dual Beneficial Effect of Interloop Disulfide Bond for Single Domain Antibody Fragments

(2011) Jochen Govaert et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Neutralization ofClostridium difficileToxin A with Single-domain Antibodies Targeting the Cell Receptor Binding Domain

(2011) Greg Hussack et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Isolation of functional single domain antibody by whole cell immunization: Implications for cancer treatment

(2011) Toya Nath Baral et al.   JOURNAL OF IMMUNOLOGICAL METHODS

Analysis and Modeling of the Variable Region of Camelid Single-Domain Antibodies

(2011) A. Sircar et al.   JOURNAL OF IMMUNOLOGY

Nanobodies With In Vitro Neutralizing Activity Protect Mice Against H5N1 Influenza Virus Infection

(2011) Lorena Itatí Ibañez et al.   JOURNAL OF INFECTIOUS DISEASES

Constraining Enzyme Conformational Change by an Antibody Leads to Hyperbolic Inhibition

(2011) David Oyen et al.   JOURNAL OF MOLECULAR BIOLOGY

TAFIa inhibiting nanobodies as profibrinolytic tools and discovery of a new TAFIa conformation

(2011) M. L. V. HENDRICKX et al.   JOURNAL OF THROMBOSIS AND HAEMOSTASIS

Biotechnological applications of recombinant single-domain antibody fragments

(2011) Ario de Marco   Microbial Cell Factories

Structure of a nanobody-stabilized active state of the β2 adrenoceptor

(2011) Søren G. F. Rasmussen et al.   NATURE

Fluorescent fusion protein knockout mediated by anti-GFP nanobody

(2011) Emmanuel Caussinus et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Engineered Single-Domain Antibodies with High Protease Resistance and Thermal Stability

(2011) Greg Hussack et al.   PLoS One

Enhancement of Polymeric Immunoglobulin Receptor Transcytosis by Biparatopic VHH

(2011) Chris D. Emmerson et al.   PLoS One

Development of Cys38 knock-out and humanized version of NbAahII10 nanobody with improved neutralization of AahII Scorpion toxin

(2011) R. Ben Abderrazek et al.   PROTEIN ENGINEERING DESIGN & SELECTION

A Case Of Convergence: Why Did a Simple Alternative to Canonical Antibodies Arise in Sharks and Camels?

(2011) Martin F. Flajnik et al.   PLOS BIOLOGY

Novel antibody derivatives for proteome and high-content analysis

(2010) Katrin Schmidthals et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Intracellular antibodies and cancer: New technologies offer therapeutic opportunities

(2010) David Pérez-Martínez et al.   BIOESSAYS

Kinetic analysis of novel mono- and multivalent VHH-fragments and their application for molecular imaging of brain tumours

(2010) U Iqbal et al.   BRITISH JOURNAL OF PHARMACOLOGY

Contributions of Conventional and Heavy-Chain IgG to Immunity in Fetal, Neonatal, and Adult Alpacas

(2010) L. P. Daley-Bauer et al.   Clinical and Vaccine Immunology

A bispecific nanobody to provide full protection against lethal scorpion envenoming

(2010) Issam Hmila et al.   FASEB JOURNAL

The pre-B cell receptor checkpoint

(2010) Inga-Lill Mårtensson et al.   FEBS LETTERS

Single Domain Intracellular Antibodies from Diverse Libraries

(2010) Tomoyuki Tanaka et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Generation of a Family-specific Phage Library of Llama Single Chain Antibody Fragments That Neutralize HIV-1

(2010) Willie W. L. Koh et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

A Novel Promiscuous Class of Camelid Single-Domain Antibody Contributes to the Antigen-Binding Repertoire

(2010) N. Deschacht et al.   JOURNAL OF IMMUNOLOGY

Structure and Properties of a Complex of α-Synuclein and a Single-Domain Camelid Antibody

(2010) Erwin J. De Genst et al.   JOURNAL OF MOLECULAR BIOLOGY

Crystal Structure of the Neutralizing Llama VHH D7 and Its Mode of HIV-1 gp120 Interaction

(2010) Andreas Hinz et al.   PLoS One

Analysis of camelid IgG for antivenom development: Immunoreactivity and preclinical neutralisation of venom-induced pathology by IgG subclasses, and the effect of heat treatment

(2010) Darren A.N. Cook et al.   TOXICON

Llama VHH antibody fragments against GFAP: better diffusion in fixed tissues than classical monoclonal antibodies

(2009) Claire Perruchini et al.   ACTA NEUROPATHOLOGICA

Substrate-dependent modulation of enzyme activity by allosteric effector antibodies

(2009) John N. Barlow et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

A one-step exclusion-binding procedure for the purification of functional heavy-chain and mammalian-type γ-globulins from camelid sera

(2009) Michel R. Blanc et al.   BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY

Differential tumor-targeting abilities of three single-domain antibody formats

(2009) Andrea Bell et al.   CANCER LETTERS

Effector Functions of Camelid Heavy-Chain Antibodies in Immunity to West Nile Virus

(2009) L. P. Daley et al.   Clinical and Vaccine Immunology

Engineering of recombinant crystallization chaperones

(2009) Shohei Koide   CURRENT OPINION IN STRUCTURAL BIOLOGY

Single domain antibodies: promising experimental and therapeutic tools in infection and immunity

(2009) Janusz Wesolowski et al.   MEDICAL MICROBIOLOGY AND IMMUNOLOGY

An Unfolded CH1 Domain Controls the Assembly and Secretion of IgG Antibodies

(2009) Matthias J. Feige et al.   MOLECULAR CELL

Origin and evolution of the adaptive immune system: genetic events and selective pressures

(2009) Martin F. Flajnik et al.   NATURE REVIEWS GENETICS

Modulation of protein properties in living cells using nanobodies

(2009) Axel Kirchhofer et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Immunological applications of single-domain llama recombinant antibodies isolated from a naïve library

(2009) Ana Monegal et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Crystal Structure of the N-Terminal Domain of the Secretin GspD from ETEC Determined with the Assistance of a Nanobody

(2009) Konstantin V. Korotkov et al.   STRUCTURE

Passive immunization with llama single-domain antibody fragments reduces foot-and-mouth disease transmission between pigs

(2009) M.M. Harmsen et al.   VACCINE

Single-domain antibodies as building blocks for novel therapeutics

(2008) D SAERENS et al.   CURRENT OPINION IN PHARMACOLOGY

Porcine IgG: structure, genetics, and evolution

(2008) J. E. Butler et al.   IMMUNOGENETICS

Nanobodies, a promising tool for species-specific diagnosis of Taenia solium cysticercosis

(2008) N. Deckers et al.   INTERNATIONAL JOURNAL FOR PARASITOLOGY

General Strategy to Humanize a Camelid Single-domain Antibody and Identification of a Universal Humanized Nanobody Scaffold

(2008) Cécile Vincke et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Disulfide Bond Introduction for General Stabilization of Immunoglobulin Heavy-Chain Variable Domains

(2008) Dirk Saerens et al.   JOURNAL OF MOLECULAR BIOLOGY

A Fluorescent Two-hybrid Assay for Direct Visualization of Protein Interactions in Living Cells

(2008) Kourosh Zolghadr et al.   MOLECULAR & CELLULAR PROTEOMICS

VHH, bivalent domains and chimeric Heavy chain-only antibodies with high neutralizing efficacy for scorpion toxin AahI′

(2008) Issam Hmila et al.   MOLECULAR IMMUNOLOGY

Sequence determinants of protein aggregation in human VH domains

(2008) K. Dudgeon et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Single-domain antibody fragments with high conformational stability

(2002) Mireille Dumoulin et al.   PROTEIN SCIENCE