Protein stability: a crystallographer's perspective

Biostructural Science Inspired by Next-Generation X-Ray Sources

(2015) Sol M. Gruner et al.   Annual Review of Biophysics

The art of CHO cell engineering: A comprehensive retrospect and future perspectives

(2015) Simon Fischer et al.   BIOTECHNOLOGY ADVANCES

ATGme: Open-source web application for rare codon identification and custom DNA sequence optimization

(2015) Edward Daniel et al.   BMC BIOINFORMATICS

Post-expression strategies for structural investigations of membrane proteins

(2015) Linda Columbus   CURRENT OPINION IN STRUCTURAL BIOLOGY

Hepatitis B Virus Core Protein Phosphorylation Sites Affect Capsid Stability and Transient Exposure of the C-terminal Domain

(2015) Lisa Selzer et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Adaptations of protein structure and function to temperature: there is more than one way to 'skin a cat'

(2015) P. A. Fields et al.   JOURNAL OF EXPERIMENTAL BIOLOGY

A Native-Like SOSIP.664 Trimer Based on an HIV-1 Subtype BenvGene

(2015) Pavel Pugach et al.   JOURNAL OF VIROLOGY

Architecture of the synaptotagmin–SNARE machinery for neuronal exocytosis

(2015) Qiangjun Zhou et al.   NATURE

Design and structure of two HIV-1 clade C SOSIP.664 trimers that increase the arsenal of native-like Env immunogens

(2015) Jean-Philippe Julien et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The Use of Affinity Tags to Overcome Obstacles in Recombinant Protein Expression and Purification

(2015) Chinthaka Amarasinghe et al.   PROTEIN AND PEPTIDE LETTERS

Can template-based protein models guide the design of sequence fitness for enhanced thermal stability of single domain antibodies?

(2015) Mark A. Olson et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Enabling X-ray free electron laser crystallography for challenging biological systems from a limited number of crystals

(2015) Monarin Uervirojnangkoorn et al.   eLife

Formulation screening by differential scanning fluorimetry: how often does it work?

(2015) Marko Ristic et al.   Acta Crystallographica Section F-Structural Biology Communications

Origin and use of crystallization phase diagrams

(2015) Bernhard Rupp   Acta Crystallographica Section F-Structural Biology Communications

The potential of future light sources to explore the structure and function of matter

(2015) Edgar Weckert   IUCrJ

A Maltose-Binding Protein Fusion Construct Yields a Robust Crystallography Platform for MCL1

(2015) Matthew C. Clifton et al.   PLoS One

Fluorinated Proteins: From Design and Synthesis to Structure and Stability

(2014) E. Neil G. Marsh   ACCOUNTS OF CHEMICAL RESEARCH

Optimization of a multi-stage, multi-subunit malaria vaccine candidate for the production inPichia pastorisby the identification and removal of protease cleavage sites

(2014) Holger Spiegel et al.   BIOTECHNOLOGY AND BIOENGINEERING

High-Resolution Microtubule Structures Reveal the Structural Transitions in αβ-Tubulin upon GTP Hydrolysis

(2014) Gregory M. Alushin et al.   CELL

A disulfide polymerized protein crystal

(2014) Esben M. Quistgaard   CHEMICAL COMMUNICATIONS

New trends and affinity tag designs for recombinant protein purification

(2014) David W Wood   CURRENT OPINION IN STRUCTURAL BIOLOGY

Stabilization of protein–protein interactions by small molecules

(2014) Fabrizio Giordanetto et al.   DRUG DISCOVERY TODAY

Predicting aggregation-prone sequences in proteins

(2014) Greet De Baets et al.   Essays in Biochemistry

Forces stabilizing proteins

(2014) C. Nick Pace et al.   FEBS LETTERS

Protein Splicing: How Inteins Escape from Precursor Proteins

(2014) Kenneth V. Mills et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Enigmatic Distribution, Evolution, and Function of Inteins

(2014) Olga Novikova et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Nanoscale high-content analysis using compositional heterogeneities of single proteoliposomes

(2014) Signe Mathiasen et al.   NATURE METHODS

Atomic Resolution Structure of a Protein Prepared by Non-Enzymatic His-Tag Removal. Crystallographic and NMR Study of GmSPI-2 Inhibitor

(2014) Edyta Kopera et al.   PLoS One

Comparative Studies on Detergent-Assisted Apocytochrome b6 Reconstitution into Liposomal Bilayers Monitored by Zetasizer Instruments

(2014) Michał A. Surma et al.   PLoS One

Nature's recipe for splitting inteins

(2014) A. S. Aranko et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Advantages of proteins being disordered

(2014) Zhirong Liu et al.   PROTEIN SCIENCE

Contribution of hydrogen bonds to protein stability

(2014) C. Nick Pace et al.   PROTEIN SCIENCE

Improving the chances of successful protein structure determination with a random forest classifier

(2014) Samad Jahandideh et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Optimization of protein buffer cocktails using Thermofluor

(2013) Linda Reinhard et al.   Acta Crystallographica Section F-Structural Biology and Crystallization Communications

Computational and Theoretical Methods for Protein Folding

(2013) Mario Compiani et al.   BIOCHEMISTRY

Pupylation as a signal for proteasomal degradation in bacteria

(2013) Frank Striebel et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH

Tertiary and quaternary effects in the allosteric regulation of animal hemoglobins

(2013) Luca Ronda et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Disulfide by Design 2.0: a web-based tool for disulfide engineering in proteins

(2013) Douglas B Craig et al.   BMC BIOINFORMATICS

New concepts and aids to facilitate crystallization

(2013) Magdalena A Bukowska et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Variations on the theme: allosteric control in hemoglobin

(2013) Maurizio Brunori   FEBS Journal

Protein disulfide engineering

(2013) Alan A. Dombkowski et al.   FEBS LETTERS

Structure, function and biosynthesis of O2-tolerant hydrogenases

(2013) Johannes Fritsch et al.   NATURE REVIEWS MICROBIOLOGY

SCMCRYS: Predicting Protein Crystallization Using an Ensemble Scoring Card Method with Estimating Propensity Scores of P-Collocated Amino Acid Pairs

(2013) Phasit Charoenkwan et al.   PLoS One

Optimization of protein purification and characterization using Thermofluor screens

(2013) Stephane Boivin et al.   PROTEIN EXPRESSION AND PURIFICATION

Statistical approaches to maximize recombinant protein expression in Escherichia coli: A general review

(2013) Christos P. Papaneophytou et al.   PROTEIN EXPRESSION AND PURIFICATION

Hepatitis C Virus E2 Envelope Glycoprotein Core Structure

(2013) L. Kong et al.   SCIENCE

High-Throughput Thermal Scanning for Protein Stability: Making a Good Technique More Robust

(2013) Shane A. Seabrook et al.   ACS Combinatorial Science

The Ubiquitin Code

(2012) David Komander et al.   Annual Review of Biochemistry

MoRFpred, a computational tool for sequence-based prediction and characterization of short disorder-to-order transitioning binding regions in proteins

(2012) Fatemeh Miri Disfani et al.   BIOINFORMATICS

Time-resolved structural studies at synchrotrons and X-ray free electron lasers: opportunities and challenges

(2012) Richard Neutze et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

RFCRYS: Sequence-based protein crystallization propensity prediction by means of random forest

(2012) Samad Jahandideh et al.   JOURNAL OF THEORETICAL BIOLOGY

Solution Structure, Conformational Dynamics, and CD4-Induced Activation in Full-Length, Glycosylated, Monomeric HIV gp120

(2012) Miklos Guttman et al.   JOURNAL OF VIROLOGY

X-ray structures of LeuT in substrate-free outward-open and apo inward-open states

(2012) Harini Krishnamurthy et al.   NATURE

Stabilizing Additives Added during Cell Lysis Aid in the Solubilization of Recombinant Proteins

(2012) David J. Leibly et al.   PLoS One

N-Terminal T4 Lysozyme Fusion Facilitates Crystallization of a G Protein Coupled Receptor

(2012) Yaozhong Zou et al.   PLoS One

Structural basis for the enhanced stability of highly fluorinated proteins

(2012) B. C. Buer et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Tafamidis, a potent and selective transthyretin kinetic stabilizer that inhibits the amyloid cascade

(2012) C. E. Bulawa et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Enhanced Crystallizability by Protein Engineering Approaches: A General Overview

(2012) Alessia Ruggiero et al.   PROTEIN AND PEPTIDE LETTERS

X-ray lasers for structural and dynamic biology

(2012) J C H Spence et al.   REPORTS ON PROGRESS IN PHYSICS

The Ebola Virus Interferon Antagonist VP24 Directly Binds STAT1 and Has a Novel, Pyramidal Fold

(2012) Adrianna P. P. Zhang et al.   PLoS Pathogens

A thermal stability assay can help to estimate the crystallization likelihood of biological samples

(2011) Florine Dupeux et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Hydrogenases for biological hydrogen production

(2011) Dong-Hoon Kim et al.   BIORESOURCE TECHNOLOGY

Recent Advances in the Therapeutic Perspectives of Nutlin-3

(2011) Paola Secchiero et al.   CURRENT PHARMACEUTICAL DESIGN

Macromolecular NMR spectroscopy for the non-spectroscopist

(2011) Ann H. Kwan et al.   FEBS Journal

Macromolecular NMR spectroscopy for the non-spectroscopist: beyond macromolecular solution structure determination

(2011) Michael Bieri et al.   FEBS Journal

Novel Strategies for Drug Discovery Based on Intrinsically Disordered Proteins (IDPs)

(2011) Jihua Wang et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Contribution of Hydrophobic Interactions to Protein Stability

(2011) C. Nick Pace et al.   JOURNAL OF MOLECULAR BIOLOGY

Crystallization chaperone strategies for membrane proteins

(2011) Raquel L. Lieberman et al.   METHODS

Conformational changes in the G protein Gs induced by the β2 adrenergic receptor

(2011) Ka Young Chung et al.   NATURE

Quantitation of protein-protein interactions by thermal stability shift analysis

(2011) Curtis J. Layton et al.   PROTEIN SCIENCE

Insights into [FeFe]-Hydrogenase Structure, Mechanism, and Maturation

(2011) David W. Mulder et al.   STRUCTURE

Proteome-wide post-translational modification statistics: frequency analysis and curation of the swiss-prot database

(2011) George A. Khoury et al.   Scientific Reports

Application of protein engineering to enhance crystallizability and improve crystal properties

(2010) Zygmunt S. Derewenda   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Synchrotron radiation circular dichroism (SRCD) spectroscopy: an enhanced method for examining protein conformations and protein interactions

(2010) B.A. Wallace et al.   BIOCHEMICAL SOCIETY TRANSACTIONS

Understanding protein non-folding

(2010) Vladimir N. Uversky et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Hydrogen exchange mass spectrometry for studying protein structure and dynamics

(2010) Lars Konermann et al.   CHEMICAL SOCIETY REVIEWS

Proteins that switch folds

(2010) Philip N Bryan et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Modulation of Integrin Activation by an Entropic Spring in the β-Knee

(2010) Benoit J. Smagghe et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

ESPRIT: An automated, library-based method for mapping and soluble expression of protein domains from challenging targets

(2010) Hayretin Yumerefendi et al.   JOURNAL OF STRUCTURAL BIOLOGY

Sequence-Specific Ni(II)-Dependent Peptide Bond Hydrolysis for Protein Engineering. Combinatorial Library Determination of Optimal Sequences

(2010) Artur Krȩżel et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Mass spectrometry guided in situ proteolysis to obtain crystals for X-ray structure determination

(2010) Tarun Gheyi et al.   JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY

Local Conformational Stability of HIV-1 gp120 in Unliganded and CD4-Bound States as Defined by Amide Hydrogen/Deuterium Exchange

(2010) L. Kong et al.   JOURNAL OF VIROLOGY

Protein trans-splicing and its use in structural biology: opportunities and limitations

(2010) Gerrit Volkmann et al.   Molecular BioSystems

PCDDB: the protein circular dichroism data bank, a repository for circular dichroism spectral and metadata

(2010) Lee Whitmore et al.   NUCLEIC ACIDS RESEARCH

SVMCRYS: An SVM Approach for the Prediction of Protein Crystallization Propensity from Protein Sequence

(2010) Krishna Kandaswamy et al.   PROTEIN AND PEPTIDE LETTERS

Synthetic symmetrization in the crystallization and structure determination of CelA fromThermotoga maritima

(2010) G. Jason Forse et al.   PROTEIN SCIENCE

New surface contacts formed upon reductive lysine methylation: Improving the probability of protein crystallization

(2010) Pawel Sledz et al.   PROTEIN SCIENCE

XANNpred: Neural nets that predict the propensity of a protein to yield diffraction-quality crystals

(2010) Ian M. Overton et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

Functional significance of eIF5A and its hypusine modification in eukaryotes

(2009) M. H. Park et al.   AMINO ACIDS

Meta prediction of protein crystallization propensity

(2009) Marcin J. Mizianty et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Chaperone-fusion expression plasmid vectors for improved solubility of recombinant proteins in Escherichia coli

(2009) Christos A. Kyratsous et al.   GENE

The Three-dimensional Structure of a Mycobacterial DapD Provides Insights into DapD Diversity and Reveals Unexpected Particulars about the Enzymatic Mechanism

(2009) Linda Schuldt et al.   JOURNAL OF MOLECULAR BIOLOGY

Mini-beam collimator enables microcrystallography experiments on standard beamlines

(2009) Robert F. Fischetti et al.   JOURNAL OF SYNCHROTRON RADIATION

In Situ Proteolysis to Generate Crystals for Structure Determination: An Update

(2009) Amy Wernimont et al.   PLoS One

Insights into Protein Aggregation by NMR Characterization of Insoluble SH3 Mutants Solubilized in Salt-Free Water

(2009) Jingxian Liu et al.   PLoS One

Increased levels of recombinant human proteins with the Escherichia coli strain Rosetta(DE3)

(2009) Hanna Tegel et al.   PROTEIN EXPRESSION AND PURIFICATION

CRYSTALP2: sequence-based protein crystallization propensity prediction

(2009) Lukasz Kurgan et al.   BMC STRUCTURAL BIOLOGY

Protein crystallography with a micrometre-sized synchrotron-radiation beam

(2008) Rouslan Moukhametzianov et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

ParCrys: a Parzen window density estimation approach to protein crystallization propensity prediction

(2008) Ian M. Overton et al.   BIOINFORMATICS

Flexible nets: disorder and induced fit in the associations of p53 and 14-3-3 with their partners

(2008) Christopher J Oldfield et al.   BMC GENOMICS

Fiber Formation across the Bacterial Outer Membrane by the Chaperone/Usher Pathway

(2008) Han Remaut et al.   CELL

Function and regulation of protein neddylation. ‘Protein Modifications: Beyond the Usual Suspects’ Review Series

(2008) Gwénaël Rabut et al.   EMBO REPORTS

X-ray beam stabilization at BL-17A, the protein microcrystallography beamline of the Photon Factory

(2008) Noriyuki Igarashi et al.   JOURNAL OF SYNCHROTRON RADIATION

Structure of a β1-adrenergic G-protein-coupled receptor

(2008) Tony Warne et al.   NATURE

Crystal structure of a stable dimer reveals the molecular basis of serpin polymerization

(2008) Masayuki Yamasaki et al.   NATURE

Protein secondary structure analyses from circular dichroism spectroscopy: Methods and reference databases

(2007) Lee Whitmore et al.   BIOPOLYMERS

The use of systematic N- and C-terminal deletions to promote production and structural studies of recombinant proteins

(2007) Susanne Gräslund et al.   PROTEIN EXPRESSION AND PURIFICATION

Combining the polymerase incomplete primer extension method for cloning and mutagenesis with microscreening to accelerate structural genomics efforts

(2007) Heath E. Klock et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

On the use of DXMS to produce more crystallizable proteins: Structures of the T. maritima proteins TM0160 and TM1171

(2004) Glen Spraggon et al.   PROTEIN SCIENCE