Current strategies for protein production and purification enabling membrane protein structural biology

A technique to increase protein yield in a rabbit reticulocyte lysate translation system

(2016) Maria Anastasina et al.   BIOTECHNIQUES

Cryo-Electron Microscopy and the Amazing Race to Atomic Resolution

(2015) Elad Binshtein et al.   BIOCHEMISTRY

Solution structure of the transmembrane 2 domain of the human melanocortin-4 receptor in sodium dodecyl sulfate (SDS) micelles and the functional implication of the D90N mutant

(2015) Ji-Hye Yun et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Membrane proteins — do we catch up with the breathless pace of soluble protein structural biology?

(2015) Bjørn Panyella Pedersen et al.   BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS

Crystal Structure of Antagonist Bound Human Lysophosphatidic Acid Receptor 1

(2015) Jill E. Chrencik et al.   CELL

Structural Insights into the Dynamic Process of β 2 -Adrenergic Receptor Signaling

(2015) Aashish Manglik et al.   CELL

Structure of the Angiotensin Receptor Revealed by Serial Femtosecond Crystallography

(2015) Haitao Zhang et al.   CELL

Membrane protein structural biology using X-ray free electron lasers

(2015) Richard Neutze et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Post-expression strategies for structural investigations of membrane proteins

(2015) Linda Columbus   CURRENT OPINION IN STRUCTURAL BIOLOGY

Affordable uniform isotope labeling with 2H, 13C and 15N in insect cells

(2015) Agnieszka Sitarska et al.   JOURNAL OF BIOMOLECULAR NMR

An economic approach to efficient isotope labeling in insect cells using homemade 15N-, 13C- and 2H-labeled yeast extracts

(2015) Christian Opitz et al.   JOURNAL OF BIOMOLECULAR NMR

Methyl labeling and TROSY NMR spectroscopy of proteins expressed in the eukaryote Pichia pastoris

(2015) Lindsay Clark et al.   JOURNAL OF BIOMOLECULAR NMR

Escherichia coli EDA is a novel fusion expression partner to improve solubility of aggregation-prone heterologous proteins

(2015) Yoon-Sik Kang et al.   JOURNAL OF BIOTECHNOLOGY

Solid-state NMR and membrane proteins

(2015) Stanley J. Opella   JOURNAL OF MAGNETIC RESONANCE

Characterization of fast-tumbling isotropic bicelles by PFG diffusion NMR

(2015) Jobst Liebau et al.   MAGNETIC RESONANCE IN CHEMISTRY

Electron cryomicroscopy observation of rotational states in a eukaryotic V-ATPase

(2015) Jianhua Zhao et al.   NATURE

An atomic structure of human γ-secretase

(2015) Xiao-chen Bai et al.   NATURE

Two disparate ligand-binding sites in the human P2Y1 receptor

(2015) Dandan Zhang et al.   NATURE

The PURE system for the cell-free synthesis of membrane proteins

(2015) Yutetsu Kuruma et al.   Nature Protocols

Use of adenylate kinase as a solubility tag for high level expression of T4 DNA ligase in Escherichia coli

(2015) Xinxin Liu et al.   PROTEIN EXPRESSION AND PURIFICATION

Membrane proteins in their native habitat as seen by solid-state NMR spectroscopy

(2015) Leonid S. Brown et al.   PROTEIN SCIENCE

Escherichia coli as host for membrane protein structure determination: a global analysis

(2015) Georges Hattab et al.   Scientific Reports

A comprehensive review of the lipid cubic phase orin mesomethod for crystallizing membrane and soluble proteins and complexes

(2015) Martin Caffrey   Acta Crystallographica Section F-Structural Biology Communications

Effect of C-Terminal Protein Tags on Pentitol and L-Arabinose Transport by Ambrosiozyma monospora Lat1 and Lat2 Transporters in Saccharomyces cerevisiae

(2014) J. Londesborough et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Small expression tags enhance bacterial expression of the first three transmembrane segments of the apelin receptor

(2014) Aditya Pandey et al.   Biochemistry and Cell Biology

GPCR structures in drug design, emerging opportunities with new structures

(2014) Christofer S. Tautermann   BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

Single particle electron cryo-microscopy of a mammalian ion channel

(2014) Maofu Liao et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Wheat germ systems for cell-free protein expression

(2014) Matthias Harbers   FEBS LETTERS

Conformational Dynamics of a Seven Transmembrane Helical Protein Anabaena Sensory Rhodopsin Probed by Solid-State NMR

(2014) Daryl B. Good et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

High-resolution structure of the human GPR40 receptor bound to allosteric agonist TAK-875

(2014) Ankita Srivastava et al.   NATURE

Crystal structure of the human OX2 orexin receptor bound to the insomnia drug suvorexant

(2014) Jie Yin et al.   NATURE

Structure of class C GPCR metabotropic glutamate receptor 5 transmembrane domain

(2014) Andrew S. Doré et al.   NATURE

Screening and large-scale expression of membrane proteins in mammalian cells for structural studies

(2014) April Goehring et al.   Nature Protocols

Expression and Functional Characterization of Membrane-Integrated Mammalian Corticotropin Releasing Factor Receptors 1 and 2 in Escherichia coli

(2014) Roberto Jappelli et al.   PLoS One

A stress-responsive Escherichia coli protein, CysQ is a highly effective solubility enhancer for aggregation-prone heterologous proteins

(2014) Jong-Hwan Lee et al.   PROTEIN EXPRESSION AND PURIFICATION

Mistic's membrane association and its assistance in overexpression of a human GPCR are independent processes

(2014) Jacopo Marino et al.   PROTEIN SCIENCE

The recombinant expression systems for structure determination of eukaryotic membrane proteins

(2014) Yuan He et al.   Protein & Cell

Membrane proteins, detergents and crystals: what is the state of the art?

(2014) Patrick J. Loll   Acta Crystallographica Section F-Structural Biology Communications

Solid State NMR Strategy for Characterizing Native Membrane Protein Structures

(2013) Dylan T. Murray et al.   ACCOUNTS OF CHEMICAL RESEARCH

Structural features of the apelin receptor N-terminal tail and first transmembrane segment implicated in ligand binding and receptor trafficking

(2013) David N. Langelaan et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Cell-free protein expression based on extracts from CHO cells

(2013) Andreas K. Brödel et al.   BIOTECHNOLOGY AND BIOENGINEERING

Detrimental effect of the 6 His C-terminal tag on YedY enzymatic activity and influence of the TAT signal sequence on YedY synthesis

(2013) Monique Sabaty et al.   BMC BIOCHEMISTRY

The Dynamic Process of β2-Adrenergic Receptor Activation

(2013) Rie Nygaard et al.   CELL

Detergent-mediated protein aggregation

(2013) Chris Neale et al.   CHEMISTRY AND PHYSICS OF LIPIDS

Targeted expression, purification, and cleavage of fusion proteins from inclusion bodies in Escherichia coli

(2013) Peter M. Hwang et al.   FEBS LETTERS

Quantitative characterization of electron detectors for transmission electron microscopy

(2013) Rachel S. Ruskin et al.   JOURNAL OF STRUCTURAL BIOLOGY

Optimized Phospholipid Bilayer Nanodiscs Facilitate High-Resolution Structure Determination of Membrane Proteins

(2013) Franz Hagn et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Activation and allosteric modulation of a muscarinic acetylcholine receptor

(2013) Andrew C. Kruse et al.   NATURE

Solid-state NMR spectroscopy structure determination of a lipid-embedded heptahelical membrane protein

(2013) Shenlin Wang et al.   NATURE METHODS

Lipid bilayer preparations of membrane proteins for oriented and magic-angle spinning solid-state NMR samples

(2013) Nabanita Das et al.   Nature Protocols

Using Haloarcula marismortui Bacteriorhodopsin as a Fusion Tag for Enhancing and Visible Expression of Integral Membrane Proteins in Escherichia coli

(2013) Min-Feng Hsu et al.   PLoS One

Effect of Signal Peptide on Stability and Folding of Escherichia coli Thioredoxin

(2013) Pranveer Singh et al.   PLoS One

The Fh8 tag: A fusion partner for simple and cost-effective protein purification in Escherichia coli

(2013) Sofia J. Costa et al.   PROTEIN EXPRESSION AND PURIFICATION

High-yield membrane protein expression fromE. coliusing an engineered outer membrane protein F fusion

(2013) Pin-Chuan Su et al.   PROTEIN SCIENCE

OptimizedE. coliexpression strain LOBSTR eliminates common contaminants from His-tag purification

(2013) Kasper R. Andersen et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

Noise models and cryo-EM drift correction with a direct-electron camera

(2013) H. Shigematsu et al.   ULTRAMICROSCOPY

CURRENT PROGRESS IN STRUCTURE-BASED RATIONAL DRUG DESIGN MARKS A NEW MINDSET IN DRUG DISCOVERY

(2013) Valère Lounnas et al.   Computational and Structural Biotechnology Journal

The novel Fh8 and H fusion partners for soluble protein expression in Escherichia coli: a comparison with the traditional gene fusion technology

(2012) Sofia J. Costa et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Topogenesis of heterologously expressed fragments of the human Y4 GPCR

(2012) Jacopo Marino et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Cell-free protein synthesis of membrane (1,3)-β-d-glucan (curdlan) synthase: Co-translational insertion in liposomes and reconstitution in nanodiscs

(2012) Agalya Periasamy et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

A novel and simple method of production and biophysical characterization of a mini-membrane protein, Ost4p: A Subunit of yeast oligosaccharyl transferase

(2012) Amit Kumar et al.   BIOPOLYMERS

Overexpression of membrane proteins in mammalian cells for structural studies

(2012) Juni Andréll et al.   MOLECULAR MEMBRANE BIOLOGY

Structure of the δ-opioid receptor bound to naltrindole

(2012) Sébastien Granier et al.   NATURE

Structure and dynamics of the M3 muscarinic acetylcholine receptor

(2012) Andrew C. Kruse et al.   NATURE

The Ability to Enhance the Solubility of Its Fusion Partners Is an Intrinsic Property of Maltose-Binding Protein but Their Folding Is Either Spontaneous or Chaperone-Mediated

(2012) Sreejith Raran-Kurussi et al.   PLoS One

Folding and activity of mutant cystathionine β-synthase depends on the position and nature of the purification tag: Characterization of the R266K CBS mutant

(2012) Tomas Majtan et al.   PROTEIN EXPRESSION AND PURIFICATION

A systematic approach to increase the efficiency of membrane protein production in cell-free expression systems

(2012) Stefan Haberstock et al.   PROTEIN EXPRESSION AND PURIFICATION

A PagP fusion protein system for the expression of intrinsically disordered proteins in Escherichia coli

(2012) Peter M. Hwang et al.   PROTEIN EXPRESSION AND PURIFICATION

Rapid screening of membrane protein expression in transiently transfected insect cells

(2012) Hao Chen et al.   PROTEIN EXPRESSION AND PURIFICATION

Fusion Partner Toolchest for the Stabilization and Crystallization of G Protein-Coupled Receptors

(2012) Eugene Chun et al.   STRUCTURE

Movies of Ice-Embedded Particles Enhance Resolution in Electron Cryo-Microscopy

(2012) Melody G. Campbell et al.   STRUCTURE

Solution NMR Structure of Proteorhodopsin

(2011) Sina Reckel et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Optimization of purification and refolding of the human chemokine receptor CXCR1 improves the stability of proteoliposomes for structure determination

(2011) Sang Ho Park et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Advances in the production of membrane proteins in Pichia pastoris

(2011) Ana Ramón et al.   Biotechnology Journal

Solution NMR studies of polytopic α-helical membrane proteins

(2011) Daniel Nietlispach et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Bilayer Hydrophobic Thickness and Integral Membrane Protein Function

(2011) Larisa E. Cybulski et al.   CURRENT PROTEIN & PEPTIDE SCIENCE

A novel Escherichia coli solubility enhancer protein for fusion expression of aggregation-prone heterologous proteins

(2011) Jong-Am Song et al.   ENZYME AND MICROBIAL TECHNOLOGY

Hydrogen exchange during cell-free incorporation of deuterated amino acids and an approach to its inhibition

(2011) Marco Tonelli et al.   JOURNAL OF BIOMOLECULAR NMR

Surface Display of Heme- and Diflavin-Containing Cytochrome P450 BM3 in Escherichia coli: A Whole-Cell Biocatalyst for Oxidation

(2011) Sung-Kun Yim   JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY

Thermostabilisation of an Agonist-Bound Conformation of the Human Adenosine A2A Receptor

(2011) Guillaume Lebon et al.   JOURNAL OF MOLECULAR BIOLOGY

Efficient expression screening of human membrane proteins in transiently transfected Human Embryonic Kidney 293S cells

(2011) Sarika Chaudhary et al.   METHODS

Structure of a nanobody-stabilized active state of the β2 adrenoceptor

(2011) Søren G. F. Rasmussen et al.   NATURE

Overcoming barriers to membrane protein structure determination

(2011) Roslyn M Bill et al.   NATURE BIOTECHNOLOGY

Heterologous Expression of Membrane Proteins: Choosing the Appropriate Host

(2011) Florent Bernaudat et al.   PLoS One

Stabilized G protein binding site in the structure of constitutively active metarhodopsin-II

(2011) X. Deupi et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

One-step purification of a functional, constitutively activated form of visual arrestin

(2011) Li Huang et al.   PROTEIN EXPRESSION AND PURIFICATION

Expression and purification of the membrane protein p7 from hepatitis C virus

(2010) Gabriel A. Cook et al.   BIOPOLYMERS

Combinatorial Method for Overexpression of Membrane Proteins inEscherichia coli

(2010) Shani Leviatan et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

EspA is a novel fusion partner for expression of foreign proteins in Escherichia coli

(2010) Yan Cheng et al.   JOURNAL OF BIOTECHNOLOGY

Sedimentation velocity to characterize surfactants and solubilized membrane proteins

(2010) Christine Ebel   METHODS

Recent Advances in Lentiviral Vector Development and Applications

(2010) Janka Mátrai et al.   MOLECULAR THERAPY

Structure determination of the seven-helix transmembrane receptor sensory rhodopsin II by solution NMR spectroscopy

(2010) Antoine Gautier et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Advances in cell-free protein synthesis for the functional and structural analysis of membrane proteins

(2010) Friederike Junge et al.   New Biotechnology

Purification of transmembrane proteins from Saccharomyces cerevisiae for X-ray crystallography

(2010) Kathleen M. Clark et al.   PROTEIN EXPRESSION AND PURIFICATION

Ribonuclease S-peptide as a carrier in fusion proteins

(2010) Jin-Soo Kim et al.   PROTEIN SCIENCE

Prediction of the human membrane proteome

(2010) Linn Fagerberg et al.   PROTEOMICS

Structures of membrane proteins

(2010) Kutti R. Vinothkumar et al.   QUARTERLY REVIEWS OF BIOPHYSICS

Decorating microbes: surface display of proteins on Escherichia coli

(2010) Edwin van Bloois et al.   TRENDS IN BIOTECHNOLOGY

Production of heterologous proteins using the fission-yeast (Schizosaccharomyces pombe) expression system

(2009) Kaoru Takegawa et al.   BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY

Computational analysis of membrane proteins: the largest class of drug targets

(2009) Yalini Arinaminpathy et al.   DRUG DISCOVERY TODAY

A Human Embryonic Kidney 293T Cell Line Mutated at the Golgi α-Mannosidase II Locus

(2009) Max Crispin et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Thermostabilization of the Neurotensin Receptor NTS1

(2009) Yoko Shibata et al.   JOURNAL OF MOLECULAR BIOLOGY

G-protein-coupled receptors, cholesterol and palmitoylation: facts about fats

(2009) Bice Chini et al.   JOURNAL OF MOLECULAR ENDOCRINOLOGY

Production and isotope labeling of antimicrobial peptides inEscherichia coliby means of a novel fusion partner that enables high-yield insoluble expression and fast purification

(2009) Verica Vidovic et al.   JOURNAL OF PEPTIDE SCIENCE

Integral Membrane Proteins in Nanodiscs Can Be Studied by Solution NMR Spectroscopy

(2009) Julian M. Glück et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Lipid bilayer regulation of membrane protein function: gramicidin channels as molecular force probes

(2009) J. A. Lundbaek et al.   Journal of the Royal Society Interface

Strategies for successful recombinant expression of disulfide bond-dependent proteins in Escherichia coli

(2009) Ario de Marco   Microbial Cell Factories

Emerging roles for lipids in shaping membrane-protein function

(2009) Rob Phillips et al.   NATURE

HaloTag7: A genetically engineered tag that enhances bacterial expression of soluble proteins and improves protein purification

(2009) Rachel Friedman Ohana et al.   PROTEIN EXPRESSION AND PURIFICATION

Production of recombinant proteins in Escherichia coli using an N-terminal tag derived from sortase

(2009) Jill Caswell et al.   PROTEIN EXPRESSION AND PURIFICATION

The Dock tag, an affinity tool for the purification of recombinant proteins, based on the interaction between dockerin and cohesin domains from Clostridium josui cellulosome

(2009) Yoshiko Kamezaki et al.   PROTEIN EXPRESSION AND PURIFICATION

Design of an expression system for detecting folded protein domains and mapping macromolecular interactions by NMR

(2009) Jeffrey R. Huth et al.   PROTEIN SCIENCE

Advanced method for high-throughput expression of mutated eukaryotic membrane proteins in Saccharomyces cerevisiae

(2008) Keisuke Ito et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Overcoming the challenges of membrane protein crystallography

(2008) Elisabeth P Carpenter et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Hyper-acidic protein fusion partners improve solubility and assist correct folding of recombinant proteins expressed in Escherichia coli

(2008) Zhurong Zou et al.   JOURNAL OF BIOTECHNOLOGY

Selecting Optimum Eukaryotic Integral Membrane Proteins for Structure Determination by Rapid Expression and Solubilization Screening

(2008) Min Li et al.   JOURNAL OF MOLECULAR BIOLOGY

Determination of the molecular mass and dimensions of membrane proteins by size exclusion chromatography

(2008) Edmund R.S. Kunji et al.   METHODS

An integrated cell-free metabolic platform for protein production and synthetic biology

(2008) Michael C Jewett et al.   Molecular Systems Biology

GFP-based optimization scheme for the overexpression and purification of eukaryotic membrane proteins in Saccharomyces cerevisiae

(2008) David Drew et al.   Nature Protocols

Conformational thermostabilization of the  1-adrenergic receptor in a detergent-resistant form

(2008) M. J. Serrano-Vega et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Tuning Escherichia coli for membrane protein overexpression

(2008) S. Wagner et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Effective high-throughput overproduction of membrane proteins in Escherichia coli

(2008) Euan Gordon et al.   PROTEIN EXPRESSION AND PURIFICATION

High-level expression and purification of the second transmembrane domain of wild-type and mutant human melanocortin-4 receptor for solid-state NMR structural studies

(2008) Tae-Joon Park et al.   PROTEIN EXPRESSION AND PURIFICATION

Codon optimization can improve expression of human genes in Escherichia coli: A multi-gene study

(2008) Nicola A. Burgess-Brown et al.   PROTEIN EXPRESSION AND PURIFICATION

BacMam system for high-level expression of recombinant soluble and membrane glycoproteins for structural studies

(2008) Abhiram Dukkipati et al.   PROTEIN EXPRESSION AND PURIFICATION

Mocr: A novel fusion tag for enhancing solubility that is compatible with structural biology applications

(2008) James DelProposto et al.   PROTEIN EXPRESSION AND PURIFICATION

Rationalizing α-helical membrane protein crystallization

(2008) Simon Newstead et al.   PROTEIN SCIENCE

Production of membrane proteins using cell-free expression systems

(2008) Daniel Schwarz et al.   PROTEOMICS

An efficient in vitro translation system from mammalian cells lacking the translational inhibition caused by eIF2 phosphorylation

(2008) V. V. Zeenko et al.   RNA

Solution Structure of the C-terminal Dimerization Domain of SARS Coronavirus Nucleocapsid Protein Solved by the SAIL-NMR Method

(2007) Mitsuhiro Takeda et al.   JOURNAL OF MOLECULAR BIOLOGY