Megabodies expand the nanobody toolkit for protein structure determination by single-particle cryo-EM
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Title
Megabodies expand the nanobody toolkit for protein structure determination by single-particle cryo-EM
Authors
Keywords
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Journal
NATURE METHODS
Volume 18, Issue 1, Pages 60-68
Publisher
Springer Science and Business Media LLC
Online
2021-01-07
DOI
10.1038/s41592-020-01001-6
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Related references
Note: Only part of the references are listed.- Development of imaging scaffolds for cryo-electron microscopy
- (2020) Todd O Yeates et al. CURRENT OPINION IN STRUCTURAL BIOLOGY
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- (2020) Takanori Nakane et al. NATURE
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- (2019) Tomasz Uchański et al. Scientific Reports
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- (2019) Dmitry Lyumkis JOURNAL OF BIOLOGICAL CHEMISTRY
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- (2019) Robert M. Glaeser Annual Review of Biophysics
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- Cryo-Electron Microscopy Methodology: Current Aspects and Future Directions
- (2019) Radostin Danev et al. TRENDS IN BIOCHEMICAL SCIENCES
- A 3.8 Å resolution cryo-EM structure of a small protein bound to an imaging scaffold
- (2019) Yuxi Liu et al. Nature Communications
- Cryo-Electron Microscopy: Moving Beyond X-Ray Crystal Structures for Drug Receptors and Drug Development
- (2019) Javier García-Nafría et al. Annual Review of Pharmacology and Toxicology
- Single particle cryo-EM reconstruction of 52 kDa streptavidin at 3.2 Angstrom resolution
- (2019) Xiao Fan et al. Nature Communications
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- (2019) Dimitry Tegunov et al. NATURE METHODS
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- (2019) Przemyslaw Dutka et al. STRUCTURE
- Nanodiscs: A Controlled Bilayer Surface for the Study of Membrane Proteins
- (2018) Mark A. McLean et al. Annual Review of Biophysics
- Locking the Elbow: Improved Antibody Fab Fragments as Chaperones for Structure Determination
- (2018) Lucas J. Bailey et al. JOURNAL OF MOLECULAR BIOLOGY
- Cryo-EM in drug discovery: achievements, limitations and prospects
- (2018) Jean-Paul Renaud et al. NATURE REVIEWS DRUG DISCOVERY
- Comparative analysis of nanobody sequence and structure data
- (2018) Laura S. Mitchell et al. PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
- Automated map sharpening by maximization of detail and connectivity
- (2018) Thomas C. Terwilliger et al. Acta Crystallographica Section D-Structural Biology
- Approaches to altering particle distributions in cryo-electron microscopy sample preparation
- (2018) Ieva Drulyte et al. Acta Crystallographica Section D-Structural Biology
- Real-space refinement in PHENIX for cryo-EM and crystallography
- (2018) Pavel V. Afonine et al. Acta Crystallographica Section D-Structural Biology
- Structure of a human synaptic GABAA receptor
- (2018) Shaotong Zhu et al. NATURE
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- (2018) Alex J. Noble et al. NATURE METHODS
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- (2018) Katrien Willegems et al. STRUCTURE
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- (2018) Michael Luke Carlson et al. eLife
- Cryo-EM structure of the benzodiazepine-sensitive α1β1γ2S tri-heteromeric GABAA receptor in complex with GABA
- (2018) Swastik Phulera et al. eLife
- Conformational transitions of the serotonin 5-HT3 receptor
- (2018) Lucie Polovinkin et al. NATURE
- Cryo-EM structure of the human α1β3γ2 GABAA receptor in a lipid bilayer
- (2018) Duncan Laverty et al. NATURE
- GABAA receptor signalling mechanisms revealed by structural pharmacology
- (2018) Simonas Masiulis et al. NATURE
- New tools for automated high-resolution cryo-EM structure determination in RELION-3
- (2018) Jasenko Zivanov et al. eLife
- A Bayesian approach to beam-induced motion correction in cryo-EM single-particle analysis
- (2018) Jasenko Zivanov et al. IUCrJ
- Structural basis of MsbA-mediated lipopolysaccharide transport
- (2017) Wei Mi et al. NATURE
- Cryo-EM structures of the TMEM16A calcium-activated chloride channel
- (2017) Shangyu Dang et al. NATURE
- Opening of the human epithelial calcium channel TRPV6
- (2017) Luke L. McGoldrick et al. NATURE
- MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy
- (2017) Shawn Q Zheng et al. NATURE METHODS
- cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination
- (2017) Ali Punjani et al. NATURE METHODS
- Addressing preferred specimen orientation in single-particle cryo-EM through tilting
- (2017) Yong Zi Tan et al. NATURE METHODS
- Structural basis for GABAA receptor potentiation by neurosteroids
- (2017) Paul S Miller et al. NATURE STRUCTURAL & MOLECULAR BIOLOGY
- Measuring the effects of particle orientation to improve the efficiency of electron cryomicroscopy
- (2017) Katerina Naydenova et al. Nature Communications
- Cryo-EM structure of haemoglobin at 3.2 Å determined with the Volta phase plate
- (2017) Maryam Khoshouei et al. Nature Communications
- Structural basis for GABAA receptor potentiation by neurosteroids
- (2017) Paul S Miller et al. NATURE STRUCTURAL & MOLECULAR BIOLOGY
- Recent developments in the CCP-EM software suite
- (2017) Tom Burnley et al. Acta Crystallographica Section D-Structural Biology
- ATSAS 2.8: a comprehensive data analysis suite for small-angle scattering from macromolecular solutions
- (2017) D. Franke et al. JOURNAL OF APPLIED CRYSTALLOGRAPHY
- Gctf: Real-time CTF determination and correction
- (2016) Kai Zhang JOURNAL OF STRUCTURAL BIOLOGY
- Unravelling biological macromolecules with cryo-electron microscopy
- (2016) Rafael Fernandez-Leiro et al. NATURE
- Inhibition of RAS function through targeting an allosteric regulatory site
- (2016) Russell Spencer-Smith et al. Nature Chemical Biology
- A saposin-lipoprotein nanoparticle system for membrane proteins
- (2016) Jens Frauenfeld et al. NATURE METHODS
- Emerging Molecular Mechanisms of Signal Transduction in Pentameric Ligand-Gated Ion Channels
- (2016) Ákos Nemecz et al. NEURON
- Design of a molecular support for cryo-EM structure determination
- (2016) Thomas G. Martin et al. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- Helicobacter pylori adhesin HopQ engages in a virulence-enhancing interaction with human CEACAMs
- (2016) Anahita Javaheri et al. Nature Microbiology
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- (2016) Francesca Coscia et al. Scientific Reports
- The styrene–maleic acid copolymer: a versatile tool in membrane research
- (2015) Jonas M. Dörr et al. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
- CTFFIND4: Fast and accurate defocus estimation from electron micrographs
- (2015) Alexis Rohou et al. JOURNAL OF STRUCTURAL BIOLOGY
- DATASW, a tool for HPLC–SAXS data analysis
- (2015) Alexander V. Shkumatov et al. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
- Crystal structure of a human GABAA receptor
- (2014) Paul S. Miller et al. NATURE
- A general protocol for the generation of Nanobodies for structural biology
- (2014) Els Pardon et al. Nature Protocols
- Volta potential phase plate for in-focus phase contrast transmission electron microscopy
- (2014) R. Danev et al. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- The Resolution Revolution
- (2014) W. Kuhlbrandt SCIENCE
- Nanobodies: Natural Single-Domain Antibodies
- (2013) Serge Muyldermans Annual Review of Biochemistry
- Exploiting structure similarity in refinement: automated NCS and target-structure restraints inBUSTER
- (2012) Oliver S. Smart et al. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
- Fabs Enable Single Particle cryoEM Studies of Small Proteins
- (2012) Shenping Wu et al. STRUCTURE
- A Versatile and Efficient High-Throughput Cloning Tool for Structural Biology
- (2011) Eric R. Geertsma et al. BIOCHEMISTRY
- XDS
- (2010) Wolfgang Kabsch ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
- Features and development ofCoot
- (2010) P. Emsley et al. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
- Structure and Properties of a Complex of α-Synuclein and a Single-Domain Camelid Antibody
- (2010) Erwin J. De Genst et al. JOURNAL OF MOLECULAR BIOLOGY
- Circular permutation: a different way to engineer enzyme structure and function
- (2010) Ying Yu et al. TRENDS IN BIOTECHNOLOGY
- MolProbity: all-atom structure validation for macromolecular crystallography
- (2009) Vincent B. Chen et al. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
- Directed evolution of a secretory leader for the improved expression of heterologous proteins and full-length antibodies inSaccharomyces cerevisiae
- (2009) J. Andy Rakestraw et al. BIOTECHNOLOGY AND BIOENGINEERING
- Histamine Action on Vertebrate GABAAReceptors
- (2008) Arunesh Saras et al. JOURNAL OF BIOLOGICAL CHEMISTRY
- Modular Design of Cys-loop Ligand-gated Ion Channels: Functional 5-HT3and GABA ρ1 Receptors Lacking the Large Cytoplasmic M3M4 Loop
- (2008) Michaela Jansen et al. JOURNAL OF GENERAL PHYSIOLOGY
- Structural Insights into the Substrate Specificity and Function of Escherichia coli K12 YgjK, a Glucosidase Belonging to the Glycoside Hydrolase Family 63
- (2008) Yuma Kurakata et al. JOURNAL OF MOLECULAR BIOLOGY
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