A mutagenesis and screening strategy to generate optimally thermostabilized membrane proteins for structural studies
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Title
A mutagenesis and screening strategy to generate optimally thermostabilized membrane proteins for structural studies
Authors
Keywords
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Journal
Nature Protocols
Volume 11, Issue 8, Pages 1554-1571
Publisher
Springer Nature
Online
2016-07-28
DOI
10.1038/nprot.2016.088
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- (2014) L. Chen et al. SCIENCE
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- (2013) Yoko Shibata et al. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
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- A crystal clear solution for determining G-protein-coupled receptor structures
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- High throughput platforms for structural genomics of integral membrane proteins
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- (2011) Jan Steyaert et al. CURRENT OPINION IN STRUCTURAL BIOLOGY
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