Organoid and Spheroid Tumor Models: Techniques and Applications

The Organoid Cell Atlas

(2021) Christoph Bock et al.   NATURE BIOTECHNOLOGY

Lung-on-a-chip: the future of respiratory disease models and pharmacological studies

(2020) Jesus Shrestha et al.   CRITICAL REVIEWS IN BIOTECHNOLOGY

Issues with cancer spheroid models in therapeutic drug screening

(2020) Eleonore Fröhlich   CURRENT PHARMACEUTICAL DESIGN

Modeling medulloblastoma in vivo and with human cerebellar organoids

(2020) Claudio Ballabio et al.   Nature Communications

On-chip recapitulation of clinical bone marrow toxicities and patient-specific pathophysiology

(2020) David B. Chou et al.   Nature Biomedical Engineering

Genome-Scale CRISPR Screening in Human Intestinal Organoids Identifies Drivers of TGF-β Resistance

(2020) Till Ringel et al.   Cell Stem Cell

Developing Organoids from Ovarian Cancer as Experimental and Preclinical Models

(2020) Nina Maenhoudt et al.   Stem Cell Reports

Patient-derived ovarian cancer organoids capture the genomic profiles of primary tumours applicable for drug sensitivity and resistance testing

(2020) Yoshiko Nanki et al.   Scientific Reports

Establishment of patient-derived cancer organoids for drug-screening applications

(2020) Else Driehuis et al.   Nature Protocols

Promising Applications of Tumor Spheroids and Organoids for Personalized Medicine

(2020) Zarema Gilazieva et al.   Cancers

Establishment of human fetal hepatocyte organoids and CRISPR–Cas9-based gene knockin and knockout in organoid cultures from human liver

(2020) Delilah Hendriks et al.   Nature Protocols

Probing the Tumor Suppressor Function of BAP1 in CRISPR-Engineered Human Liver Organoids

(2019) Benedetta Artegiani et al.   Cell Stem Cell

3D model for CAR‐mediated cytotoxicity using patient‐derived colorectal cancer organoids

(2019) Theresa E Schnalzger et al.   EMBO JOURNAL

An organoid platform for ovarian cancer captures intra- and interpatient heterogeneity

(2019) Oded Kopper et al.   NATURE MEDICINE

Oral mucosal organoids as a potential platform for personalized cancer therapy

(2019) Else Driehuis et al.   Cancer Discovery

CEA expression heterogeneity and plasticity confer resistance to the CEA-targeting bispecific immunotherapy antibody cibisatamab (CEA-TCB) in patient-derived colorectal cancer organoids

(2019) Reyes Gonzalez-Exposito et al.   Journal for ImmunoTherapy of Cancer

The Tumor Microenvironment Innately Modulates Cancer Progression

(2019) Dominique C. Hinshaw et al.   CANCER RESEARCH

Antitumor activity of dual blockade of PD-L1 and MEK in NSCLC patients derived three-dimensional spheroid cultures

(2019) Carminia Maria Della Corte et al.   JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH

Establishment of an organoid bank of biliary tract and pancreatic cancers and its application for personalized therapy and future treatment

(2019) Yoshimasa Saito   JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY

Organoids-on-a-chip

(2019) Sunghee Estelle Park et al.   SCIENCE

Human Organoids: Tools for Understanding Biology and Treating Diseases

(2019) Frans Schutgens et al.   Annual Review of Pathology-Mechanisms of Disease

Patient-derived organoids as a potential model to predict response to PD-1/PD-L1 checkpoint inhibitors

(2019) Giosue Scognamiglio et al.   BRITISH JOURNAL OF CANCER

Patient-Derived Organoids Predict Chemoradiation Responses of Locally Advanced Rectal Cancer

(2019) Ye Yao et al.   Cell Stem Cell

Modeling breast cancer using CRISPR/Cas9-mediated engineering of human breast organoids

(2019) Johanna F Dekkers et al.   JNCI-Journal of the National Cancer Institute

A rectal cancer organoid platform to study individual responses to chemoradiation

(2019) Karuna Ganesh et al.   NATURE MEDICINE

Multispecies liver-on-a-chip for improved drug toxicity testing

(2019) Iain Dickson   Nature Reviews Gastroenterology & Hepatology

Reproducing human and cross-species drug toxicities using a Liver-Chip

(2019) Kyung-Jin Jang et al.   Science Translational Medicine

The Tumor-on-Chip: Recent Advances in the Development of Microfluidic Systems to Recapitulate the Physiology of Solid Tumors

(2019) Grissel Trujillo-de Santiago et al.   Materials

Model of Patient-Specific Immune-Enhanced Organoids for Immunotherapy Screening: Feasibility Study

(2019) Konstantinos I. Votanopoulos et al.   ANNALS OF SURGICAL ONCOLOGY

A Patient-Derived Glioblastoma Organoid Model and Biobank Recapitulates Inter- and Intra-tumoral Heterogeneity

(2019) Fadi Jacob et al.   CELL

Spheroid Culture System Methods and Applications for Mesenchymal Stem Cells

(2019) Na-Eun Ryu et al.   Cells

A Living Biobank of Breast Cancer Organoids Captures Disease Heterogeneity

(2018) Norman Sachs et al.   CELL

Human Pancreatic Tumor Organoids Reveal Loss of Stem Cell Niche Factor Dependence during Disease Progression

(2018) Takashi Seino et al.   Cell Stem Cell

Generation of Intestinal Organoids Suitable for Pharmacokinetic Studies from Human Induced Pluripotent Stem Cells

(2018) Daichi Onozato et al.   DRUG METABOLISM AND DISPOSITION

Generation of human brain region–specific organoids using a miniaturized spinning bioreactor

(2018) Xuyu Qian et al.   Nature Protocols

Patient-derived organoids model treatment response of metastatic gastrointestinal cancers

(2018) Georgios Vlachogiannis et al.   SCIENCE

Current Status of 3-D Organoids as Pre-Clinical Models

(2018) Garima Kaushik et al.   STEM CELLS

Prediction of Drug-Induced Hepatotoxicity Using Long-Term Stable Primary Hepatic 3D Spheroid Cultures in Chemically Defined Conditions

(2018) Sabine U Vorrink et al.   TOXICOLOGICAL SCIENCES

Personalized Cancer Medicine: An Organoid Approach

(2018) Hamidreza Aboulkheyr Es et al.   TRENDS IN BIOTECHNOLOGY

Organoid profiling identifies common responders to chemotherapy in pancreatic cancer

(2018) Hervé Tiriac et al.   Cancer Discovery

A Cytotoxic Three-Dimensional-Spheroid, High-Throughput Assay Using Patient-Derived Glioma Stem Cells

(2018) Victor Quereda et al.   SLAS Discovery

Generation of Tumor-Reactive T Cells by Co-culture of Peripheral Blood Lymphocytes and Tumor Organoids

(2018) Krijn K. Dijkstra et al.   CELL

3D microfluidic ex vivo culture of organotypic tumor spheroids to model immune checkpoint blockade

(2018) Amir R. Aref et al.   LAB ON A CHIP

Intra-tumour diversification in colorectal cancer at the single-cell level

(2018) Sophie F. Roerink et al.   NATURE

Organoid cultures recapitulate esophageal adenocarcinoma heterogeneity providing a model for clonality studies and precision therapeutics

(2018) Xiaodun Li et al.   Nature Communications

Drug Screening of Human GBM Spheroids in Brain Cancer Chip

(2018) Metin Akay et al.   Scientific Reports

Organoid Models of Human Liver Cancers Derived from Tumor Needle Biopsies

(2018) Sandro Nuciforo et al.   Cell Reports

3D tumor spheroids as in vitro models to mimic in vivo human solid tumors resistance to therapeutic drugs

(2018) Ana S. Nunes et al.   BIOTECHNOLOGY AND BIOENGINEERING

Organoid Modeling of the Tumor Immune Microenvironment

(2018) James T. Neal et al.   CELL

Comparative Analysis and Refinement of Human PSC-Derived Kidney Organoid Differentiation with Single-Cell Transcriptomics

(2018) Haojia Wu et al.   Cell Stem Cell

Applicability of tumor spheroids for in vitro chemosensitivity assays

(2018) Gerhard Hamilton et al.   Expert Opinion on Drug Metabolism & Toxicology

Patient-derived, three-dimensional spheroid cultures provide a versatile translational model for the study of organ-confined prostate cancer

(2018) Johannes Linxweiler et al.   JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY

A Microfluidic Spheroid Culture Device with a Concentration Gradient Generator for High-Throughput Screening of Drug Efficacy

(2018) Wanyoung Lim et al.   MOLECULES

Mouse Models for Cancer Immunotherapy Research

(2018) Brian Olson et al.   Cancer Discovery

Characterization of genetic intratumor heterogeneity in colorectal cancer and matching patient-derived spheroid cultures

(2017) Sigrid S. Árnadóttir et al.   Molecular Oncology

Nanoparticles Penetrate into the Multicellular Spheroid-on-Chip: Effect of Surface Charge, Protein Corona, and Exterior Flow

(2017) Ke Huang et al.   MOLECULAR PHARMACEUTICS

Organoid cystogenesis reveals a critical role of microenvironment in human polycystic kidney disease

(2017) Nelly M. Cruz et al.   NATURE MATERIALS

Human primary liver cancer–derived organoid cultures for disease modeling and drug screening

(2017) Laura Broutier et al.   NATURE MEDICINE

Genetic dissection of colorectal cancer progression by orthotopic transplantation of engineered cancer organoids

(2017) Arianna Fumagalli et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Disease Modeling in Stem Cell-Derived 3D Organoid Systems

(2017) Devanjali Dutta et al.   TRENDS IN MOLECULAR MEDICINE

Personalized In Vitro and In Vivo Cancer Models to Guide Precision Medicine

(2017) Chantal Pauli et al.   Cancer Discovery

Ex Vivo Profiling of PD-1 Blockade Using Organotypic Tumor Spheroids

(2017) Russell W. Jenkins et al.   Cancer Discovery

Application of 3D tumoroid systems to define immune and cytotoxic therapeutic responses based on tumoroid and tissue slice culture molecular signatures

(2017) Niklas K. Finnberg et al.   Oncotarget

Spheroids Formation on Non-Adhesive Surfaces by Liquid Overlay Technique: Considerations and Practical Approaches

(2017) Elisabete C. Costa et al.   Biotechnology Journal

Short-term spheroid culture of primary colorectal cancer cells as an in vitro model for personalizing cancer medicine

(2017) Maria Jeppesen et al.   PLoS One

Human engineered heart tissue as a model system for drug testing

(2016) Alexandra Eder et al.   ADVANCED DRUG DELIVERY REVIEWS

Organoids as Models for Neoplastic Transformation

(2016) James T. Neal et al.   Annual Review of Pathology-Mechanisms of Disease

Modeling Development and Disease with Organoids

(2016) Hans Clevers   CELL

A Colorectal Tumor Organoid Library Demonstrates Progressive Loss of Niche Factor Requirements during Tumorigenesis

(2016) Masayuki Fujii et al.   Cell Stem Cell

Organoids: A historical perspective of thinking in three dimensions

(2016) Marina Simian et al.   JOURNAL OF CELL BIOLOGY

Testing chemotherapy efficacy in HER2 negative breast cancer using patient-derived spheroids

(2016) Kathrin Halfter et al.   Journal of Translational Medicine

Three-dimensional culture systems in cancer research: Focus on tumor spheroid model

(2016) Sritama Nath et al.   PHARMACOLOGY & THERAPEUTICS

Zika virus impairs growth in human neurospheres and brain organoids

(2016) P. P. Garcez et al.   SCIENCE

Human Salivary Gland Stem Cells Functionally Restore Radiation Damaged Salivary Glands

(2016) Sarah Pringle et al.   STEM CELLS

Kidney-on-a-Chip Technology for Drug-Induced Nephrotoxicity Screening

(2016) Martijn J. Wilmer et al.   TRENDS IN BIOTECHNOLOGY

Drug testing and flow cytometry analysis on a large number of uniform sized tumor spheroids using a microfluidic device

(2016) Bishnubrata Patra et al.   Scientific Reports

Long-Term Culture of Genome-Stable Bipotent Stem Cells from Adult Human Liver

(2015) Meritxell Huch et al.   CELL

Prospective Derivation of a Living Organoid Biobank of Colorectal Cancer Patients

(2015) Marc van de Wetering et al.   CELL

In Vitro Expansion of Human Gastric Epithelial Stem Cells and Their Responses to Bacterial Infection

(2015) Sina Bartfeld et al.   GASTROENTEROLOGY

Sequential cancer mutations in cultured human intestinal stem cells

(2015) Jarno Drost et al.   NATURE

Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis

(2015) Minoru Takasato et al.   NATURE

Modeling colorectal cancer using CRISPR-Cas9–mediated engineering of human intestinal organoids

(2015) Mami Matano et al.   NATURE MEDICINE

The Notch and Wnt pathways regulate stemness and differentiation in human fallopian tube organoids

(2015) Mirjana Kessler et al.   Nature Communications

Spherical Cancer Models in Tumor Biology

(2015) Louis-Bastien Weiswald et al.   NEOPLASIA

Organoid Cultures Derived from Patients with Advanced Prostate Cancer

(2014) Dong Gao et al.   CELL

Microfluidic organs-on-chips

(2014) Sangeeta N Bhatia et al.   NATURE BIOTECHNOLOGY

Oncogenic transformation of diverse gastrointestinal tissues in primary organoid culture

(2014) Xingnan Li et al.   NATURE MEDICINE

Generation of cerebral organoids from human pluripotent stem cells

(2014) Madeline A Lancaster et al.   Nature Protocols

Organogenesis in a dish: Modeling development and disease using organoid technologies

(2014) M. A. Lancaster et al.   SCIENCE

The Clinical Relevance of Cancer Cell Lines

(2013) J.-P. Gillet et al.   JNCI-Journal of the National Cancer Institute

Vascularized and functional human liver from an iPSC-derived organ bud transplant

(2013) Takanori Takebe et al.   NATURE

Cerebral organoids model human brain development and microcephaly

(2013) Madeline A. Lancaster et al.   NATURE

Advances in the formation, use and understanding of multi-cellular spheroids

(2012) Toni-Marie Achilli et al.   EXPERT OPINION ON BIOLOGICAL THERAPY

Chondrogenic differentiation of human mesenchymal stem cells: a comparison between micromass and pellet culture systems

(2010) Liangming Zhang et al.   BIOTECHNOLOGY LETTERS

Three-dimensional culture of hepatocytes for prediction of drug-induced hepatotoxicity

(2010) Qin Meng   Expert Opinion on Drug Metabolism & Toxicology

Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro

(2010) Jason R. Spence et al.   NATURE

Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche

(2009) Toshiro Sato et al.   NATURE

Sustained in vitro intestinal epithelial culture within a Wnt-dependent stem cell niche

(2009) Akifumi Ootani et al.   NATURE MEDICINE