Promising Applications of Tumor Spheroids and Organoids for Personalized Medicine

Pancreatic cancer-derived organoids – a disease modeling tool to predict drug response

(2020) Pierre-Olivier Frappart et al.   United European Gastroenterology Journal

Patient-derived organoids from multiple colorectal cancer liver metastases reveal moderate intra-patient pharmacotranscriptomic heterogeneity

(2020) Jarle Bruun et al.   CLINICAL CANCER RESEARCH

Tumor microenvironment is critical for the maintenance of cellular states found in primary glioblastomas.

(2020) Allison R Pine et al.   Cancer Discovery

Developing Organoids from Ovarian Cancer as Experimental and Preclinical Models

(2020) Nina Maenhoudt et al.   Stem Cell Reports

Metabolic Heterogeneity in Patient Tumor-Derived Organoids by Primary Site and Drug Treatment

(2020) Joe T. Sharick et al.   Frontiers in Oncology

Modeling Cell Communication in Cancer With Organoids: Making the Complex Simple

(2020) Elena Fiorini et al.   Frontiers in Cell and Developmental Biology

Cell Culture Based in vitro Test Systems for Anticancer Drug Screening

(2020) Kristina V. Kitaeva et al.   Frontiers in Bioengineering and Biotechnology

Three-dimensional bioprinted glioblastoma microenvironments model cellular dependencies and immune interactions

(2020) Min Tang et al.   CELL RESEARCH

Mouse Tumor Models for Advanced Cancer Immunotherapy

(2020) Daria S. Chulpanova et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Patient-derived ovarian cancer organoids capture the genomic profiles of primary tumours applicable for drug sensitivity and resistance testing

(2020) Yoshiko Nanki et al.   Scientific Reports

Long‐term expanding human airway organoids for disease modeling

(2019) Norman Sachs et al.   EMBO JOURNAL

Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography

(2019) Yongyang Huang et al.   Jove-Journal of Visualized Experiments

Modeling Patient-Derived Glioblastoma with Cerebral Organoids

(2019) Amanda Linkous et al.   Cell Reports

Mouse and human urothelial cancer organoids: A tool for bladder cancer research

(2019) Jasper Mullenders et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Tubuloids derived from human adult kidney and urine for personalized disease modeling

(2019) Frans Schutgens et al.   NATURE BIOTECHNOLOGY

Efficient use of patient-derived organoids as a preclinical model for gynecologic tumors

(2019) Yoshiaki Maru et al.   GYNECOLOGIC ONCOLOGY

Establishment and Morphological Characterization of Patient-Derived Organoids from Breast Cancer

(2019) S. Mazzucchelli et al.   BIOLOGICAL PROCEDURES ONLINE

Establishment and characterization of patient‐derived organoids from a young patient with cervical clear cell carcinoma

(2019) Yoshiaki Maru et al.   CANCER SCIENCE

Extracellular vesicle release from intestinal organoids is modulated by Apc mutation and other colorectal cancer progression factors

(2019) Zsuzsanna Szvicsek et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

SREBP1 drives Keratin-80-dependent cytoskeletal changes and invasive behavior in endocrine-resistant ERα breast cancer

(2019) Ylenia Perone et al.   Nature Communications

Assessing Cell Viability and Death in 3D Spheroid Cultures of Cancer Cells

(2019) Michala G. Rolver et al.   Jove-Journal of Visualized Experiments

Cancer Stem Cells: From Historical Roots to a New Perspective

(2019) Jean-Pascal Capp   Journal of Oncology

Utilizing gastric cancer organoids to assess tumor biology and personalize medicine

(2019) Miranda Lin et al.   World Journal of Gastrointestinal Oncology

Generation of complex human organoid models including vascular networks by incorporation of mesodermal progenitor cells

(2019) Philipp Wörsdörfer et al.   Scientific Reports

Emerging organoid models: leaping forward in cancer research

(2019) Han Fan et al.   Journal of Hematology & Oncology

A Living Biobank of Breast Cancer Organoids Captures Disease Heterogeneity

(2018) Norman Sachs et al.   CELL

Tumor Evolution and Drug Response in Patient-Derived Organoid Models of Bladder Cancer

(2018) Suk Hyung Lee et al.   CELL

Human gastric cancer modelling using organoids

(2018) Therese Seidlitz et al.   GUT

Three-dimensional liver-derived extracellular matrix hydrogel promotes liver organoids function

(2018) Mona Saheli et al.   JOURNAL OF CELLULAR BIOCHEMISTRY

Application of 3D cultured multicellular spheroid tumor models in tumor-targeted drug delivery system research

(2018) Bu-Wei Huang et al.   JOURNAL OF CONTROLLED RELEASE

Patient-derived organoids model treatment response of metastatic gastrointestinal cancers

(2018) Georgios Vlachogiannis et al.   SCIENCE

Current Status of 3-D Organoids as Pre-Clinical Models

(2018) Garima Kaushik et al.   STEM CELLS

Patient derived organoids to model rare prostate cancer phenotypes

(2018) Loredana Puca et al.   Nature Communications

Organoid profiling identifies common responders to chemotherapy in pancreatic cancer

(2018) Hervé Tiriac et al.   Cancer Discovery

Therapeutic Prospects of Extracellular Vesicles in Cancer Treatment

(2018) Daria S. Chulpanova et al.   Frontiers in Immunology

Organoids Provide an Important Window on Inflammation in Cancer

(2018) Kristi Baker   Cancers

Growth of Epithelial Organoids in a Defined Hydrogel

(2018) Nicolas Broguiere et al.   ADVANCED MATERIALS

Divergent Routes toward Wnt and R-spondin Niche Independency during Human Gastric Carcinogenesis

(2018) Kosaku Nanki et al.   CELL

Organoid technology and applications in cancer research

(2018) Hanxiao Xu et al.   Journal of Hematology & Oncology

Organoid cultures recapitulate esophageal adenocarcinoma heterogeneity providing a model for clonality studies and precision therapeutics

(2018) Xiaodun Li et al.   Nature Communications

Microfluidic-Based 3D Engineered Microvascular Networks and Their Applications in Vascularized Microtumor Models

(2018) Xiaolin Wang et al.   Micromachines

3D tumor spheroids as in vitro models to mimic in vivo human solid tumors resistance to therapeutic drugs

(2018) Ana S. Nunes et al.   BIOTECHNOLOGY AND BIOENGINEERING

Organoid Modeling of the Tumor Immune Microenvironment

(2018) James T. Neal et al.   CELL

A Comprehensive Human Gastric Cancer Organoid Biobank Captures Tumor Subtype Heterogeneity and Enables Therapeutic Screening

(2018) Helen H.N. Yan et al.   Cell Stem Cell

Development of extracellular matrix supported 3D culture of renal cancer cells and renal cancer stem cells

(2018) Kamila Maliszewska-Olejniczak et al.   CYTOTECHNOLOGY

MALDI Mass Spectrometry Imaging for Evaluation of Therapeutics in Colorectal Tumor Organoids

(2017) Xin Liu et al.   JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY

Advances in multicellular spheroids formation

(2017) X. Cui et al.   Journal of the Royal Society Interface

Synthetic hydrogels for human intestinal organoid generation and colonic wound repair

(2017) Ricardo Cruz-Acuña et al.   NATURE CELL BIOLOGY

Human primary liver cancer–derived organoid cultures for disease modeling and drug screening

(2017) Laura Broutier et al.   NATURE MEDICINE

Hypoxia inducible factor down-regulation, cancer and cancer stem cells (CSCs): ongoing success stories

(2017) Anthony R. Martin et al.   MedChemComm

Personalized In Vitro and In Vivo Cancer Models to Guide Precision Medicine

(2017) Chantal Pauli et al.   Cancer Discovery

Microenvironment of a tumor-organoid system enhances hepatocellular carcinoma malignancy-related hallmarks

(2017) Yang Wang et al.   Organogenesis

Spheroids Formation on Non-Adhesive Surfaces by Liquid Overlay Technique: Considerations and Practical Approaches

(2017) Elisabete C. Costa et al.   Biotechnology Journal

Three-Dimensional Cell Cultures in Drug Discovery and Development

(2017) Ye Fang et al.   SLAS Discovery

Short-term spheroid culture of primary colorectal cancer cells as an in vitro model for personalizing cancer medicine

(2017) Maria Jeppesen et al.   PLoS One

Three-dimensional spheroid culture targeting versatile tissue bioassays using a PDMS-based hanging drop array

(2017) Ching-Te Kuo et al.   Scientific Reports

Defining the optimal murine models to investigate immune checkpoint blockers and their combination with other immunotherapies

(2016) M. F. Sanmamed et al.   ANNALS OF ONCOLOGY

Organoids as a model system for studying human lung development and disease

(2016) Rohan R. Nadkarni et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

A Three-Dimensional Organoid Culture System Derived from Human Glioblastomas Recapitulates the Hypoxic Gradients and Cancer Stem Cell Heterogeneity of Tumors Found In Vivo

(2016) Christopher G. Hubert et al.   CANCER RESEARCH

A Colorectal Tumor Organoid Library Demonstrates Progressive Loss of Niche Factor Requirements during Tumorigenesis

(2016) Masayuki Fujii et al.   Cell Stem Cell

Liquid-based three-dimensional tumor models for cancer research and drug discovery

(2016) Stephanie L Ham et al.   EXPERIMENTAL BIOLOGY AND MEDICINE

Generation of Multicellular Breast Cancer Tumor Spheroids: Comparison of Different Protocols

(2016) Karolin Froehlich et al.   JOURNAL OF MAMMARY GLAND BIOLOGY AND NEOPLASIA

Testing chemotherapy efficacy in HER2 negative breast cancer using patient-derived spheroids

(2016) Kathrin Halfter et al.   Journal of Translational Medicine

Designer matrices for intestinal stem cell and organoid culture

(2016) Nikolce Gjorevski et al.   NATURE

Organoids as an in vitro model of human development and disease

(2016) Aliya Fatehullah et al.   NATURE CELL BIOLOGY

Patient-derived induced pluripotent stem cells in cancer research and precision oncology

(2016) Eirini P Papapetrou   NATURE MEDICINE

Targeting of Micelles and Liposomes Loaded with the Pro-Apoptotic Drug, NCL-240, into NCI/ADR-RES Cells in a 3D Spheroid Model

(2016) Bhushan S. Pattni et al.   PHARMACEUTICAL RESEARCH

Drug penetration and metabolism in 3D cell cultures treated in a 3D printed fluidic device: assessment of irinotecan via MALDI imaging mass spectrometry

(2016) Gabriel J. LaBonia et al.   PROTEOMICS

Targeting mutant RAS in patient-derived colorectal cancer organoids by combinatorial drug screening

(2016) Carla S Verissimo et al.   eLife

Establishment of a Novel Model for Anticancer Drug Resistance in Three-Dimensional Primary Culture of Tumor Microenvironment

(2016) Tatsuya Usui et al.   Stem Cells International

Establishment and Characterization of an In Vitro Model of Ovarian Cancer Stem-like Cells with an Enhanced Proliferative Capacity

(2015) T. Ishiguro et al.   CANCER RESEARCH

Modeling Familial Cancer with Induced Pluripotent Stem Cells

(2015) Dung-Fang Lee et al.   CELL

Prospective Derivation of a Living Organoid Biobank of Colorectal Cancer Patients

(2015) Marc van de Wetering et al.   CELL

Organoid Models of Human and Mouse Ductal Pancreatic Cancer

(2015) Sylvia F. Boj et al.   CELL

Three-dimensional imaging and uptake of the anticancer drug combretastatin in cell spheroids and photoisomerization in gels with multiphoton excitation

(2015) Kathrin M. Scherer et al.   JOURNAL OF BIOMEDICAL OPTICS

The consensus molecular subtypes of colorectal cancer

(2015) Justin Guinney et al.   NATURE MEDICINE

Modeling colorectal cancer using CRISPR-Cas9–mediated engineering of human intestinal organoids

(2015) Mami Matano et al.   NATURE MEDICINE

Preserved genetic diversity in organoids cultured from biopsies of human colorectal cancer metastases

(2015) Fleur Weeber et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Spherical Cancer Models in Tumor Biology

(2015) Louis-Bastien Weiswald et al.   NEOPLASIA

Optimization of liquid overlay technique to formulate heterogenic 3D co-cultures models

(2014) Elisabete C. Costa et al.   BIOTECHNOLOGY AND BIOENGINEERING

Organoid Cultures Derived from Patients with Advanced Prostate Cancer

(2014) Dong Gao et al.   CELL

Optimization of Aqueous Biphasic Tumor Spheroid Microtechnology for Anti-cancer Drug Testing in 3D Culture

(2014) Stephanie Lemmo et al.   Cellular and Molecular Bioengineering

A three-dimensional in vitro model of tumor cell intravasation

(2014) Seema M. Ehsan et al.   Integrative Biology

Development of an Innovative 3D Cell Culture System to Study Tumour - Stroma Interactions in Non-Small Cell Lung Cancer Cells

(2014) Arno Amann et al.   PLoS One

Three-Dimensional Lung Tumor Microenvironment Modulates Therapeutic Compound Responsiveness In Vitro – Implication for Drug Development

(2014) Jason E. Ekert et al.   PLoS One

Molecular and Cellular Heterogeneity in Breast Cancer

(2013) Ashley G. Rivenbark et al.   AMERICAN JOURNAL OF PATHOLOGY

Generation of multicellular tumor spheroids of breast cancer cells: How to go three-dimensional

(2013) Anika Nagelkerke et al.   ANALYTICAL BIOCHEMISTRY

Advances in establishment and analysis of three-dimensional tumor spheroid-based functional assays for target validation and drug evaluation

(2012) Maria Vinci et al.   BMC BIOLOGY

HIF-1 activation induces doxorubicin resistance in MCF7 3-D spheroids via P-glycoprotein expression: a potential model of the chemo-resistance of invasive micropapillary carcinoma of the breast

(2012) Sophie Doublier et al.   BMC CANCER

Concise Review: The Relevance of Human Stem Cell-Derived Organoid Models for Epithelial Translational Medicine

(2012) Robert E. Hynds et al.   STEM CELLS

The liquid overlay technique is the key to formation of co-culture spheroids consisting of primary osteoblasts, fibroblasts and endothelial cells

(2011) Wolfgang Metzger et al.   CYTOTHERAPY

Long-term Expansion of Epithelial Organoids From Human Colon, Adenoma, Adenocarcinoma, and Barrett's Epithelium

(2011) Toshiro Sato et al.   GASTROENTEROLOGY

Personalized medicine: new genomics, old lessons

(2011) Kenneth Offit   HUMAN GENETICS

Breast cancer cells produce tenascin C as a metastatic niche component to colonize the lungs

(2011) Thordur Oskarsson et al.   NATURE MEDICINE

Retaining cell-cell contact enables preparation and culture of spheroids composed of pure primary cancer cells from colorectal cancer

(2011) J. Kondo et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Simple Hanging Drop Cell Culture Protocol for Generation of 3D Spheroids

(2011) Ramsey Foty   Jove-Journal of Visualized Experiments

Multicellular tumor spheroids: An underestimated tool is catching up again

(2010) Franziska Hirschhaeuser et al.   JOURNAL OF BIOTECHNOLOGY

Microarrays for the scalable production of metabolically relevant tumour spheroids: a tool for modulating chemosensitivity traits

(2010) Heike Hardelauf et al.   LAB ON A CHIP

A Comprehensive Panel of Three-Dimensional Models for Studies of Prostate Cancer Growth, Invasion and Drug Responses

(2010) Ville Härmä et al.   PLoS One

Matrigel: A complex protein mixture required for optimal growth of cell culture

(2010) Chris S. Hughes et al.   PROTEOMICS

Engineering a scaffold-free 3D tumor model for in vitro drug penetration studies

(2009) Siew-Min Ong et al.   BIOMATERIALS

Chemoresistant Colorectal Cancer Cells, the Cancer Stem Cell Phenotype, and Increased Sensitivity to Insulin-like Growth Factor-I Receptor Inhibition

(2009) N. A. Dallas et al.   CANCER RESEARCH

Identification of Selective Inhibitors of Cancer Stem Cells by High-Throughput Screening

(2009) Piyush B. Gupta et al.   CELL

Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche

(2009) Toshiro Sato et al.   NATURE

Spheroid-based drug screen: considerations and practical approach

(2009) Juergen Friedrich et al.   Nature Protocols

Extracellular Matrix Molecules: Potential Targets in Pharmacotherapy

(2009) H. Jarvelainen et al.   PHARMACOLOGICAL REVIEWS

Modelling tissues in 3D: the next future of pharmaco-toxicology and food research?

(2008) Giovanna Mazzoleni et al.   Genes and Nutrition