Spheroids Formation on Non-Adhesive Surfaces by Liquid Overlay Technique: Considerations and Practical Approaches

Thermo- and pH-responsive nano-in-micro particles for combinatorial drug delivery to cancer cells

(2017) André F. Moreira et al.   EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES

Real-time and label-free impedimetric analysis of the formation and drug testing of tumor spheroids formed via the liquid overlay technique

(2017) Kin Fong Lei et al.   RSC Advances

3D tumor spheroids: an overview on the tools and techniques used for their analysis

(2016) Elisabete C. Costa et al.   BIOTECHNOLOGY ADVANCES

Tumor spheroid assembly on hyaluronic acid-based structures: A review

(2016) Marco P. Carvalho et al.   CARBOHYDRATE POLYMERS

Liquid-based three-dimensional tumor models for cancer research and drug discovery

(2016) Stephanie L Ham et al.   EXPERIMENTAL BIOLOGY AND MEDICINE

Evaluation of the radiosensitizing potency of chemotherapeutic agents in prostate cancer cells

(2016) Colin Rae et al.   INTERNATIONAL JOURNAL OF RADIATION BIOLOGY

Generation of Multicellular Breast Cancer Tumor Spheroids: Comparison of Different Protocols

(2016) Karolin Froehlich et al.   JOURNAL OF MAMMARY GLAND BIOLOGY AND NEOPLASIA

Co-culture of Hepatocytes and Kupffer Cells as an In Vitro Model of Inflammation and Drug-Induced Hepatotoxicity

(2016) Kelly A. Rose et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Single cell dual adherent-suspension co-culture micro-environment for studying tumor–stromal interactions with functionally selected cancer stem-like cells

(2016) Yu-Chih Chen et al.   LAB ON A CHIP

Agarose multi-wells for tumour spheroid formation and anti-cancer drug test

(2016) Yadong Tang et al.   MICROELECTRONIC ENGINEERING

Comparative analysis of tumor spheroid generation techniques for differential in vitro drug toxicity

(2016) Shreya Raghavan et al.   Oncotarget

Alginate–marine collagen–agarose composite hydrogels as matrices for biomimetic 3D cell spheroid formation

(2016) Suil Shin et al.   RSC Advances

Characterization of a functional C3A liver spheroid model

(2016) Harriet Gaskell et al.   Toxicology Research

3D tumor spheroid models for in vitro therapeutic screening: a systematic approach to enhance the biological relevance of data obtained

(2016) Michele Zanoni et al.   Scientific Reports

Characterization of primary human hepatocyte spheroids as a model system for drug-induced liver injury, liver function and disease

(2016) Catherine C. Bell et al.   Scientific Reports

Advantages and challenges of microfluidic cell culture in polydimethylsiloxane devices

(2015) Skarphedinn Halldorsson et al.   BIOSENSORS & BIOELECTRONICS

Three-dimensional imaging and uptake of the anticancer drug combretastatin in cell spheroids and photoisomerization in gels with multiphoton excitation

(2015) Kathrin M. Scherer et al.   JOURNAL OF BIOMEDICAL OPTICS

Bioreducible poly(2-ethyl-2-oxazoline)–PLA–PEI-SS triblock copolymer micelles for co-delivery of DNA minicircles and Doxorubicin

(2015) Vítor M. Gaspar et al.   JOURNAL OF CONTROLLED RELEASE

Treatment Efficiency of Free and Nanoparticle-Loaded Mitoxantrone for Magnetic Drug Targeting in Multicellular Tumor Spheroids

(2015) Annkathrin Hornung et al.   MOLECULES

Three-Dimensional Neural Spheroid Culture: AnIn VitroModel for Cortical Studies

(2015) Yu-Ting L. Dingle et al.   TISSUE ENGINEERING PART C-METHODS

Evaluation of spheroid head and neck squamous cell carcinoma cell models in comparison to monolayer cultures

(2015) LORENZ KADLETZ et al.   Oncology Letters

Characterization of macrophage - cancer cell crosstalk in estrogen receptor positive and triple-negative breast cancer

(2015) Maija Hollmén et al.   Scientific Reports

Three-Dimensional Cell Culture Systems and Their Applications in Drug Discovery and Cell-Based Biosensors

(2014) Rasheena Edmondson et al.   ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES

Optimization of liquid overlay technique to formulate heterogenic 3D co-cultures models

(2014) Elisabete C. Costa et al.   BIOTECHNOLOGY AND BIOENGINEERING

Three-dimensional models of cancer for pharmacology and cancer cell biology: Capturing tumor complexity in vitro/ex vivo

(2014) John A. Hickman et al.   Biotechnology Journal

Mesenchymal stem cell spheroids exhibit enhanced in-vitro and in-vivo osteoregenerative potential

(2014) Yuichiro Yamaguchi et al.   BMC BIOTECHNOLOGY

The effect of co-delivery of paclitaxel and curcumin by transferrin-targeted PEG-PE-based mixed micelles on resistant ovarian cancer in 3-D spheroids and in vivo tumors

(2014) Can Sarisozen et al.   EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS

Multilayer Spheroids To Quantify Drug Uptake and Diffusion in 3D

(2014) Toni-Marie Achilli et al.   MOLECULAR PHARMACEUTICS

Folate-Targeted Multifunctional Amino Acid-Chitosan Nanoparticles for Improved Cancer Therapy

(2014) Vítor M. Gaspar et al.   PHARMACEUTICAL RESEARCH

3D pancreatic carcinoma spheroids induce a matrix-rich, chemoresistant phenotype offering a better model for drug testing

(2013) Paola Longati et al.   BMC CANCER

A spheroid-based 3-D culture model for pancreatic cancer drug testing, using the acid phosphatase assay

(2013) Z. Wen et al.   BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH

Isolation, culture and evaluation of multilineage-differentiating stress-enduring (Muse) cells

(2013) Yasumasa Kuroda et al.   Nature Protocols

Multicellular Tumor Spheroids for Evaluation of Cytotoxicity and Tumor Growth Inhibitory Effects of Nanomedicines In Vitro: A Comparison of Docetaxel-Loaded Block Copolymer Micelles and Taxotere®

(2013) Andrew S. Mikhail et al.   PLoS One

Application of Concave Microwells to Pancreatic Tumor Spheroids Enabling Anticancer Drug Evaluation in a Clinically Relevant Drug Resistance Model

(2013) Sang-Eun Yeon et al.   PLoS One

Evaluation of Nanoparticle Uptake in Co-culture Cancer Models

(2013) Elisabete C. Costa et al.   PLoS One

Tumor Spheroids as an In Vitro Model for Determining the Therapeutic Response to Proton Beam Radiotherapy and Thermally Sensitive Nanocarriers

(2013) Lakmini K. Senavirathna et al.   Theranostics

Recreating the tumor microenvironment in a bilayer, hyaluronic acid hydrogel construct for the growth of prostate cancer spheroids

(2012) Xian Xu et al.   BIOMATERIALS

Synthetic 3D multicellular systems for drug development

(2012) Markus Rimann et al.   CURRENT OPINION IN BIOTECHNOLOGY

Three-dimensional cell culture: the missing link in drug discovery

(2012) Susan Breslin et al.   DRUG DISCOVERY TODAY

Advances in the formation, use and understanding of multi-cellular spheroids

(2012) Toni-Marie Achilli et al.   EXPERT OPINION ON BIOLOGICAL THERAPY

Opportunities and challenges for use of tumor spheroids as models to test drug delivery and efficacy

(2012) Geeta Mehta et al.   JOURNAL OF CONTROLLED RELEASE

The resistance of intracellular mediators to doxorubicin and cisplatin are distinct in 3D and 2D endometrial cancer

(2012) Kenny Chitcholtan et al.   Journal of Translational Medicine

Development of Multicellular Tumor Spheroid (MCTS) Culture from Breast Cancer Cell and a High Throughput Screening Method Using the MTT Assay

(2012) Wan Yong Ho et al.   PLoS One

Optimizing a 3D Culture System to Study the Interaction between Epithelial Breast Cancer and Its Surrounding Fibroblasts

(2012) Liyuan Li et al.   Journal of Cancer

Rapid Generation of In Vitro Multicellular Spheroids for the Study of Monoclonal Antibody Therapy

(2012) Yen T. Phung et al.   Journal of Cancer

Adhesion, phenotypic expression, and biosynthetic capacity of corneal keratocytes on surfaces coated with hyaluronic acid of different molecular weights

(2011) Jui-Yang Lai et al.   Acta Biomaterialia

Spheroid confrontation assay: A simple method to monitor the three-dimensional migration of different cell types in vitro

(2011) Kirsten Hattermann et al.   ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER

The liquid overlay technique is the key to formation of co-culture spheroids consisting of primary osteoblasts, fibroblasts and endothelial cells

(2011) Wolfgang Metzger et al.   CYTOTHERAPY

Induced albumin secretion from HepG2 spheroids prepared using poly(ethylene glycol) derivative with oleyl groups

(2011) Tetsushi Taguchi et al.   JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE

The Development and Characterization of a Human Mesothelioma In Vitro 3D Model to Investigate Immunotoxin Therapy

(2011) Xinran Xiang et al.   PLoS One

MCF-7 Cells as a Three-Dimensional Model for the Study of Human Breast Cancer

(2011) Jônatas Bussador do Amaral et al.   TISSUE ENGINEERING PART C-METHODS

Spinner-flask culture induces redifferentiation of de-differentiated chondrocytes

(2010) Tae-Jin Lee et al.   BIOTECHNOLOGY LETTERS

Hyaluronic acid-based hydrogels as 3D matrices for in vitro evaluation of chemotherapeutic drugs using poorly adherent prostate cancer cells

(2009) Lisa A. Gurski et al.   BIOMATERIALS

Spheroid-based drug screen: considerations and practical approach

(2009) Juergen Friedrich et al.   Nature Protocols

Recent advances in three-dimensional multicellular spheroid culture for biomedical research

(2008) Ruei-Zhen Lin et al.   Biotechnology Journal

Cellular Imaging Predictions of Clinical Drug-Induced Liver Injury

(2008) Jinghai J. Xu et al.   TOXICOLOGICAL SCIENCES

In vitro biocompatibility of three-dimensional chitosan scaffolds immobilized with chondroitin-6-sulfate

(2007) Chi-Hsiung Jou et al.   POLYMERS FOR ADVANCED TECHNOLOGIES